FastQCFastQC Report
Fri 27 May 2016
SRR939844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811259
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166400.4366011336411406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139110.3649974982020377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93240.2446435679128603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100300.043.2928241
GTATCAA165350.035.703591
ATCAACG206750.028.28953
TCAACGC209400.027.9767974
TATCAAC210450.027.9128882
CAACGCA211700.027.6280295
AACGCAG217800.026.854246
GTACATG185750.026.23841
TACATGG185450.025.7397422
ACATGGG184850.024.5840683
ACGCAGA249750.023.3618537
CGCAGAG251600.023.0769528
GAGTACT141950.021.53777912-13
CATGGGA125800.021.3424114
GCAGAGT270050.021.1665739
AGTACTT148700.019.81043612-13
GTACTTT155400.019.70419114-15
CATGGGG63800.019.7030244
ATGGGAG53100.019.474655
AGAGTAC240050.019.25709210-11
Produced by FastQC (version 0.11.5)