FastQCFastQC Report
Fri 27 May 2016
SRR939843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712729
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149660.8738101591086506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106740.6232159320009178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72310.42219171859646215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22360.13055188532453177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.1278661130861917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.10684702600353004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77150.041.6182251
GTATCAA136300.038.7980421
TATCAAC174450.031.0755482
ATCAACG176800.030.7179453
TCAACGC177550.030.6692284
CAACGCA178500.030.3731375
CATTCCG4850.030.3171869
AACGCAG182950.029.7648586
GTACATG128800.029.237181
TACATGG132100.029.0820982
ACATGGG137300.027.0495573
ACGCAGA203900.026.5205177
CGCAGAG204800.026.2395368
CATGGGA89800.024.7201524
GCAGAGT207250.024.6713649
CATGGGG41600.023.374494
GAGTACT114650.023.33045412-13
TAGGCAT6850.022.8509885
TTAGGCA7550.022.6171154
AGTACTT116400.022.61299712-13
Produced by FastQC (version 0.11.5)