##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1712729 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.030386593559168 31.0 30.0 33.0 16.0 34.0 2 29.860500989940615 31.0 30.0 33.0 25.0 34.0 3 30.103326328917184 31.0 30.0 34.0 26.0 34.0 4 33.9275051686519 35.0 35.0 37.0 28.0 37.0 5 33.94424336833206 35.0 35.0 37.0 28.0 37.0 6 34.01673586422604 36.0 35.0 37.0 29.0 37.0 7 33.95733242094926 36.0 35.0 37.0 29.0 37.0 8 33.84883364501915 35.0 35.0 37.0 28.0 37.0 9 35.091789185562924 37.0 35.0 39.0 28.0 39.0 10-11 34.95431209490819 37.0 34.0 39.0 28.0 39.0 12-13 34.86100223678118 37.0 34.0 39.0 27.0 39.0 14-15 35.43905720052618 38.0 34.0 40.0 27.0 41.0 16-17 35.31468667839454 38.0 34.0 40.0 27.0 41.0 18-19 35.32121427266077 38.0 34.0 40.0 27.0 41.0 20-21 35.55827600280021 38.0 34.0 40.0 27.0 41.0 22-23 35.44253585943836 38.0 34.0 40.0 27.0 41.0 24-25 35.213551297373954 38.0 34.0 40.0 25.5 41.0 26-27 35.070526043524694 38.0 34.0 40.0 25.5 41.0 28-29 35.078634448298594 38.0 34.0 40.0 25.0 41.0 30-31 34.767249810098384 38.0 34.0 40.0 24.5 41.0 32-33 34.32055509073531 38.0 33.0 40.0 24.0 41.0 34-35 34.0748226952425 38.0 33.0 40.0 23.5 41.0 36-37 33.79693197230852 38.0 33.0 40.0 21.5 41.0 38-39 33.51528788267146 37.0 33.0 40.0 20.0 41.0 40-41 33.21237072531615 37.0 32.0 40.0 18.0 41.0 42-43 32.69449194822999 37.0 31.0 40.0 15.0 41.0 44-45 32.15731005897605 37.0 30.5 40.0 9.0 41.0 46-47 31.56506166474673 36.5 30.0 40.0 5.0 41.0 48-49 31.052525238960747 36.0 29.5 39.0 2.0 41.0 50-51 30.38244608458197 35.5 28.5 38.5 2.0 40.0 52-53 30.509495956453122 36.0 29.0 39.0 2.0 40.0 54-55 30.582561806333636 36.0 28.5 39.0 2.0 40.0 56-57 30.29236966268452 36.0 28.0 39.0 2.0 40.0 58-59 29.92655697427906 35.0 27.5 39.0 2.0 40.0 60-61 29.53327817769186 35.0 27.0 39.0 2.0 40.0 62-63 29.116817955438368 35.0 26.0 38.0 2.0 40.0 64-65 28.594150913542073 34.0 26.0 38.0 2.0 40.0 66-67 28.02159973936332 34.0 24.5 38.0 2.0 40.0 68-69 27.495949738691877 33.5 23.0 37.0 2.0 39.0 70-71 26.943890714759895 33.0 22.0 36.5 2.0 39.0 72-73 26.292984179049924 32.0 20.5 36.0 2.0 39.0 74-75 25.931886480581575 32.0 19.5 35.5 2.0 38.0 76-77 25.369522849207318 32.0 17.5 35.0 2.0 37.5 78-79 24.69904987887751 31.0 10.5 35.0 2.0 37.0 80-81 23.957522468528296 31.0 6.0 34.5 2.0 36.0 82-83 23.20664594340377 30.0 2.0 34.0 2.0 36.0 84-85 22.454811882089928 29.5 2.0 34.0 2.0 35.0 86-87 21.618755798494682 29.0 2.0 34.0 2.0 35.0 88-89 20.684721283985965 28.5 2.0 34.0 2.0 35.0 90-91 19.36234775028624 26.0 2.0 33.0 2.0 35.0 92-93 17.896205996395224 22.0 2.0 32.0 2.0 34.0 94-95 17.334490453539352 20.0 2.0 32.0 2.0 34.0 96-97 16.951706311973467 19.5 2.0 32.0 2.0 34.0 98-99 16.06411872514566 8.0 2.0 32.0 2.0 34.0 100-101 14.445413138914564 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4282388840183877 1101 2 0.8367265930632399 1101 3 0.7927545603679 1101 4 0.6983793714638225 1101 5 0.9370593060316708 1101 6 0.862300401630435 1101 7 0.6986111084436217 1101 8 0.63491557425899 1101 9 0.8049485907615619 1101 10-11 0.930728085738096 1101 12-13 0.9816388233061062 1101 14-15 1.34377735670585 1101 16-17 1.0504504597554316 1101 18-19 1.1242897339487925 1101 20-21 0.8937269916895971 1101 22-23 1.00041269788629 1101 24-25 1.165665979603908 1101 26-27 0.9980605350424625 1101 28-29 1.3380747841066025 1101 30-31 1.2917209896375041 1101 32-33 1.763905434417822 1101 34-35 1.3789383692624497 1101 36-37 1.2648251758272622 1101 38-39 1.454373619316236 1101 40-41 1.3057080188552348 1101 42-43 1.2806846912417313 1101 44-45 1.5377312045850005 1101 46-47 1.3991254569852707 1101 48-49 1.5406948267377523 1101 50-51 1.0783535930603598 1101 52-53 1.0377098743124904 1101 54-55 1.2403421117421978 1101 56-57 1.341808495882752 1101 58-59 1.0599284865341154 1101 60-61 1.0656733293778125 1101 62-63 0.6649448720846287 1101 64-65 0.8888260978983098 1101 66-67 0.8449148587714959 1101 68-69 0.5291977701998256 1101 70-71 0.5075927852306172 1101 72-73 0.48157694829302855 1101 74-75 0.3076929117639011 1101 76-77 0.16281999225474308 1101 78-79 -0.007907960358178201 1101 80-81 -0.4086431461209443 1101 82-83 -0.42659432052384005 1101 84-85 -0.5814594697794959 1101 86-87 -0.8531751474184261 1101 88-89 -4.681601231467017 1101 90-91 -7.798181118535819 1101 92-93 -9.983056868502569 1101 94-95 -8.02958794257386 1101 96-97 -4.997389876790248 1101 98-99 -3.560783660044624 1101 100-101 -2.9604471765607574 1102 1 1.8948924885045706 1102 2 1.422427559214178 1102 3 1.1840794708469211 1102 4 1.1190644603091684 1102 5 1.1421386925596053 1102 6 1.1000194746660412 1102 7 1.0070308098151486 1102 8 1.1484079058582068 1102 9 1.5258728526357643 1102 10-11 1.56315494878789 1102 12-13 1.5666925358930897 1102 14-15 1.700535008215887 1102 16-17 1.6463270897128695 1102 18-19 1.6500906933434223 1102 20-21 1.6235216001620003 1102 22-23 1.73183219656881 1102 24-25 1.8314507238779427 1102 26-27 1.739839468898282 1102 28-29 1.459982763297198 1102 30-31 1.645290343059564 1102 32-33 1.8656316691316945 1102 34-35 1.6901659890178706 1102 36-37 1.7020442826443727 1102 38-39 1.725366119822958 1102 40-41 1.7117650420293486 1102 42-43 1.7614028859582973 1102 44-45 1.535078681106853 1102 46-47 1.261180723521317 1102 48-49 1.2964890298321414 1102 50-51 1.527255694269737 1102 52-53 1.6039797209444693 1102 54-55 1.7306096612320978 1102 56-57 1.781874707625203 1102 58-59 1.5488082919526711 1102 60-61 1.4385886729356798 1102 62-63 1.564176680746204 1102 64-65 1.3395963161268085 1102 66-67 1.5820022177337272 1102 68-69 1.5250554825165636 1102 70-71 1.7221451803390586 1102 72-73 1.411876252394105 1102 74-75 1.4487029786512764 1102 76-77 1.3875575099900281 1102 78-79 1.4101367663494742 1102 80-81 1.0877537189557387 1102 82-83 0.8664061524171771 1102 84-85 0.973396283210171 1102 86-87 0.9270917264414464 1102 88-89 0.44120398439677544 1102 90-91 -5.2855576471487495 1102 92-93 -9.89879904808503 1102 94-95 -7.575652397678246 1102 96-97 -3.758944501477771 1102 98-99 -2.2743009899547655 1102 100-101 -1.5881074697841893 1103 1 1.9984133465461298 1103 2 1.242902472842804 1103 3 1.1068866693205983 1103 4 0.8829442107345855 1103 5 0.8500827961021216 1103 6 0.7166909540658253 1103 7 0.7093429843110997 1103 8 0.8463349929514905 1103 9 0.9664596623093544 1103 10-11 0.9155424081804568 1103 12-13 0.6804918449221233 1103 14-15 0.9380480283777999 1103 16-17 0.8967584309184531 1103 18-19 0.6997818190073204 1103 20-21 0.8494964364431397 1103 22-23 0.968801979480169 1103 24-25 0.8138456601951631 1103 26-27 0.7055869035396469 1103 28-29 0.5172853982685766 1103 30-31 0.7941718982598971 1103 32-33 0.6417970380792539 1103 34-35 0.6899782924263178 1103 36-37 0.775805949225095 1103 38-39 0.6147060878526602 1103 40-41 0.45558239880757156 1103 42-43 0.4603996889284474 1103 44-45 0.2876404301915798 1103 46-47 0.5344795942478662 1103 48-49 0.41468605968827177 1103 50-51 0.40304334960929467 1103 52-53 0.28023635993792695 1103 54-55 0.19969128862342345 1103 56-57 0.31228851673158076 1103 58-59 0.36408932119002557 1103 60-61 0.6336662782571878 1103 62-63 0.4748318140417247 1103 64-65 0.29781964413175643 1103 66-67 0.3281882126803559 1103 68-69 0.1625190002807031 1103 70-71 -0.01843750413472023 1103 72-73 0.19413763536734407 1103 74-75 -0.08735358815118133 1103 76-77 -0.11613557514378314 1103 78-79 -0.22632432785977485 1103 80-81 -0.3594016979472663 1103 82-83 -0.8313185420602132 1103 84-85 -0.7949059392412323 1103 86-87 -0.630810093802971 1103 88-89 -1.4016398252252529 1103 90-91 -6.487350562118069 1103 92-93 -9.70995771358289 1103 94-95 -8.548972667878832 1103 96-97 -5.671007617034929 1103 98-99 -4.242782658598092 1103 100-101 -3.384586869567615 1104 1 1.8816778217207428 1104 2 1.1972479902800401 1104 3 0.9723124020696972 1104 4 0.7620570666634379 1104 5 0.7009998953764693 1104 6 0.787324246015352 1104 7 0.7486051918996779 1104 8 0.8580020712800476 1104 9 0.897724276759881 1104 10-11 0.9726311171471096 1104 12-13 0.8022606164323562 1104 14-15 1.0444768632803445 1104 16-17 0.9743882975508882 1104 18-19 0.9796986669321726 1104 20-21 0.8087051860174199 1104 22-23 1.0038264724559909 1104 24-25 0.9668066607620673 1104 26-27 0.9697748215714128 1104 28-29 0.9886073443448566 1104 30-31 1.1525353627048176 1104 32-33 1.1309154269615576 1104 34-35 1.2215640031524089 1104 36-37 0.8389316707668328 1104 38-39 0.8587971982408007 1104 40-41 0.8914733131213239 1104 42-43 0.8790964199703311 1104 44-45 0.8024409446419938 1104 46-47 0.6642707402166472 1104 48-49 0.5521675757066369 1104 50-51 0.4591174117546437 1104 52-53 0.27507947867390925 1104 54-55 0.26790928590101615 1104 56-57 0.2576481532649595 1104 58-59 0.1661892592775267 1104 60-61 0.19435031886917642 1104 62-63 0.27572859317732323 1104 64-65 0.21965606783086145 1104 66-67 0.3879605672811941 1104 68-69 0.13566241421634828 1104 70-71 -0.08881373408796733 1104 72-73 -0.05041088216020384 1104 74-75 0.17191927730937095 1104 76-77 0.059281898978643 1104 78-79 0.1343407255275153 1104 80-81 0.18507669197959942 1104 82-83 -0.07000849428204248 1104 84-85 -0.11496920641487662 1104 86-87 0.19865769809704403 1104 88-89 -0.19437740219423816 1104 90-91 -2.9000640051819637 1104 92-93 -7.32786021826441 1104 94-95 -5.449949122632113 1104 96-97 -3.003690996086309 1104 98-99 -2.086632185114375 1104 100-101 -1.629626117085948 1105 1 1.8199138390148093 1105 2 1.0395427072202565 1105 3 0.6497377179338457 1105 4 0.5276719727937902 1105 5 0.3447362173479078 1105 6 0.36958522700012253 1105 7 0.4434757689234985 1105 8 0.3885179152669025 1105 9 0.6439303180768832 1105 10-11 0.5353377670552462 1105 12-13 0.5076196546598268 1105 14-15 0.4110155756328382 1105 16-17 0.3734610551178008 1105 18-19 0.5659761967088457 1105 20-21 0.49891206118827114 1105 22-23 0.4221444930152174 1105 24-25 0.3513077840212375 1105 26-27 0.12592617368223813 1105 28-29 0.1324156358455042 1105 30-31 0.4915985141531465 1105 32-33 0.6005198503577205 1105 34-35 0.559445861638558 1105 36-37 0.4683368931965859 1105 38-39 0.3571371265538019 1105 40-41 0.3783189249272212 1105 42-43 0.43124836616384954 1105 44-45 0.15306160069866337 1105 46-47 0.27758189599001426 1105 48-49 0.24256668778972923 1105 50-51 0.0708324475446851 1105 52-53 0.08417657988635696 1105 54-55 0.15703001297270802 1105 56-57 0.10823788342313279 1105 58-59 0.12797019395744869 1105 60-61 0.15120067264618342 1105 62-63 0.2232101178164818 1105 64-65 0.17539098935543862 1105 66-67 0.28728664995490405 1105 68-69 0.45179573003773044 1105 70-71 0.1207099918031993 1105 72-73 0.21986677132006704 1105 74-75 -0.24785054237354842 1105 76-77 -0.06056111199737657 1105 78-79 -0.5814703118874789 1105 80-81 -0.5809616316992496 1105 82-83 -0.6530825209562039 1105 84-85 -0.5863862310838144 1105 86-87 -0.3469301306910708 1105 88-89 -0.3955192550908109 1105 90-91 -2.894528842053578 1105 92-93 -7.9736307771144865 1105 94-95 -5.681981557409012 1105 96-97 -3.178321717950231 1105 98-99 -2.118354330043063 1105 100-101 -1.6482960056458076 1106 1 1.69006831227491 1106 2 1.0835301015557874 1106 3 0.7858838323096009 1106 4 0.8152173697959029 1106 5 0.6738762668188585 1106 6 0.710163749785039 1106 7 0.8079267177152261 1106 8 -0.34549268821581336 1106 9 -0.7566407077494617 1106 10-11 -0.601296547466859 1106 12-13 -0.22724052930745842 1106 14-15 0.7946230492475834 1106 16-17 0.8440478380247356 1106 18-19 0.8879368982805147 1106 20-21 0.9011519459057027 1106 22-23 0.6384873015464905 1106 24-25 0.6729222562937167 1106 26-27 0.7298926034689615 1106 28-29 0.626882139703973 1106 30-31 0.5595211832499629 1106 32-33 0.7087038804892103 1106 34-35 0.84506876013333 1106 36-37 0.49216162148795917 1106 38-39 0.28093750095280967 1106 40-41 0.4432860191150638 1106 42-43 0.6960599096843652 1106 44-45 0.5794039827562472 1106 46-47 0.44063793912221527 1106 48-49 0.16804321602453243 1106 50-51 0.3187965968194568 1106 52-53 0.6194178966575485 1106 54-55 0.7527509106324892 1106 56-57 0.6760236320191346 1106 58-59 0.6132557293166023 1106 60-61 0.35646919394966403 1106 62-63 0.3725774777647679 1106 64-65 0.4416368312369485 1106 66-67 0.6755825284233694 1106 68-69 0.5625655374960843 1106 70-71 0.5132565784424514 1106 72-73 0.5103464195466074 1106 74-75 0.30066083998480764 1106 76-77 0.12470410974649937 1106 78-79 0.16485135252710847 1106 80-81 0.05772087883913812 1106 82-83 -0.15141422321471865 1106 84-85 -0.0824935363536845 1106 86-87 0.053508463831214215 1106 88-89 -0.01697615655742979 1106 90-91 -3.329682956177937 1106 92-93 -8.361774584043113 1106 94-95 -5.797616531843037 1106 96-97 -3.0282327196247003 1106 98-99 -2.0519652827852894 1106 100-101 -1.3692963558031543 1107 1 1.960813712100368 1107 2 1.30582140411153 1107 3 1.068643453473051 1107 4 0.9052797032835187 1107 5 0.7548810172431288 1107 6 0.6623590076504087 1107 7 0.5046905343576 1107 8 0.5901831592271805 1107 9 0.7084173082173066 1107 10-11 0.6223170678063425 1107 12-13 0.7595662783291459 1107 14-15 0.8212540934889319 1107 16-17 0.7305016451570481 1107 18-19 0.5616877531496414 1107 20-21 0.834846914785011 1107 22-23 0.4385411842066347 1107 24-25 0.49080922705201857 1107 26-27 0.4090921696444596 1107 28-29 0.39184896963048743 1107 30-31 0.6844034588482586 1107 32-33 1.0016044946133746 1107 34-35 1.000705024545212 1107 36-37 0.7127132509778917 1107 38-39 0.683035125280135 1107 40-41 0.6961137074338097 1107 42-43 0.8296428532894851 1107 44-45 0.8251210020780064 1107 46-47 0.7291110795372013 1107 48-49 0.8182111963688747 1107 50-51 0.8523420067766985 1107 52-53 0.7880176613238774 1107 54-55 0.9835275808222512 1107 56-57 0.7158023907376467 1107 58-59 0.6766651431239232 1107 60-61 0.38622612328278905 1107 62-63 0.5540192764324345 1107 64-65 0.5552123832463813 1107 66-67 0.4549813913610805 1107 68-69 0.5233002759234004 1107 70-71 0.5974159974307902 1107 72-73 0.605696276895717 1107 74-75 0.4244211358328478 1107 76-77 0.6621675259532047 1107 78-79 0.6323122127614766 1107 80-81 0.5503315858302962 1107 82-83 0.47444899311955524 1107 84-85 0.4663877076449623 1107 86-87 0.5407268751216989 1107 88-89 0.3766021356385636 1107 90-91 -2.5705125419031774 1107 92-93 -4.430816347710081 1107 94-95 -2.9576975400474765 1107 96-97 -1.51595196333321 1107 98-99 -1.0285798628804805 1107 100-101 -0.5779448681581716 1108 1 1.5097262144119235 1108 2 0.7980403545944661 1108 3 -0.4548917319492176 1108 4 -0.1280920374240324 1108 5 0.3915614821146036 1108 6 0.37322971704883656 1108 7 0.3290336971337453 1108 8 0.2634928791378428 1108 9 0.588109764724507 1108 10-11 0.4071219725702804 1108 12-13 0.4416883568793395 1108 14-15 0.36044028390416116 1108 16-17 0.11174106534794959 1108 18-19 0.26446802029426664 1108 20-21 0.19641322440534736 1108 22-23 0.18186968105580803 1108 24-25 0.2333517952250972 1108 26-27 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0.5982543462014469 2311 80-81 0.8195283451541364 2311 82-83 0.9387907830862936 2311 84-85 1.2260839291293983 2311 86-87 1.5336125152745907 2311 88-89 1.7471579856883181 2311 90-91 2.321408474752797 2311 92-93 3.2971996603745595 2311 94-95 2.9995078030072655 2311 96-97 2.3236703711068003 2311 98-99 2.2766587239193576 2311 100-101 2.192336869791589 2312 1 1.110801849255349 2312 2 0.45644051850482725 2312 3 -0.08902558024501772 2312 4 -0.4902086321110346 2312 5 -0.15115657969917606 2312 6 -0.20324914641996372 2312 7 0.10132897454718659 2312 8 0.1719114614530568 2312 9 0.03210114329774427 2312 10-11 -0.012154192185441559 2312 12-13 0.05982158567142193 2312 14-15 0.10493156756211164 2312 16-17 -0.26757724394465754 2312 18-19 -0.8012106670031685 2312 20-21 -0.048128877917086754 2312 22-23 0.12153246560671249 2312 24-25 0.154057739072897 2312 26-27 0.14317250751090427 2312 28-29 0.29859715493049066 2312 30-31 0.16535728676370098 2312 32-33 0.08812601680950394 2312 34-35 0.009252775846384509 2312 36-37 0.24331722349392493 2312 38-39 0.16781777510077944 2312 40-41 0.058719103491249314 2312 42-43 0.29623180058613485 2312 44-45 0.06874580669577313 2312 46-47 0.2502438905900668 2312 48-49 0.2202621285677857 2312 50-51 0.290190354688324 2312 52-53 0.36786978169904216 2312 54-55 0.32800390912589705 2312 56-57 0.2995349620112755 2312 58-59 0.23732605729604117 2312 60-61 0.39267009163697253 2312 62-63 0.4165673288370435 2312 64-65 0.5259448528384816 2312 66-67 0.6964762044207724 2312 68-69 0.7780680755114702 2312 70-71 0.7201729935534935 2312 72-73 0.7154904545640939 2312 74-75 0.7608041267989556 2312 76-77 0.8537175938591233 2312 78-79 0.9668105484161416 2312 80-81 1.0662076983168696 2312 82-83 1.0447264969877885 2312 84-85 1.4093473258899394 2312 86-87 1.7314382674728854 2312 88-89 2.0335428458485687 2312 90-91 2.90766790261063 2312 92-93 3.734840794786731 2312 94-95 3.557120966306041 2312 96-97 2.5145637275454824 2312 98-99 1.9824649975911797 2312 100-101 1.8547756566131035 2313 1 -7.193284661687986 2313 2 -2.4688689748037937 2313 3 -1.451068154313539 2313 4 -0.7017314318602743 2313 5 -0.4635224114198522 2313 6 -0.11606524933909412 2313 7 0.007890886836975142 2313 8 0.12106515874652501 2313 9 0.3012643802352457 2313 10-11 0.08424609127826699 2313 12-13 -0.054206052040157715 2313 14-15 -0.10913123055176044 2313 16-17 -0.20493735187891815 2313 18-19 -0.31064101397364396 2313 20-21 -0.2512730083103989 2313 22-23 -0.7727313464350445 2313 24-25 -0.08001269075620598 2313 26-27 -0.07455719348939738 2313 28-29 -0.49786704414824356 2313 30-31 0.0641863635987221 2313 32-33 0.0795148527003704 2313 34-35 0.006943909428656525 2313 36-37 0.3399775303978956 2313 38-39 0.2875869157151314 2313 40-41 -0.03195126092315803 2313 42-43 0.27848247517524527 2313 44-45 0.31840987494510387 2313 46-47 -0.290680637197557 2313 48-49 -0.076258081849506 2313 50-51 0.273436695553432 2313 52-53 -0.17888292347144485 2313 54-55 0.07873546354275263 2313 56-57 0.38262567039798867 2313 58-59 0.43470687988591905 2313 60-61 0.4045514457213031 2313 62-63 0.46523573081038805 2313 64-65 0.41514188737199476 2313 66-67 0.44116139616761885 2313 68-69 0.383463698177664 2313 70-71 0.4171772727652474 2313 72-73 0.2595270867971884 2313 74-75 0.3097427732597495 2313 76-77 0.5344958925317478 2313 78-79 0.4967734800850323 2313 80-81 0.5685175186990001 2313 82-83 0.9726577570384869 2313 84-85 0.7508674000265998 2313 86-87 1.361437040947223 2313 88-89 1.8954209291978046 2313 90-91 2.4930238676137684 2313 92-93 3.587150887730118 2313 94-95 3.3685838025784953 2313 96-97 2.4311004279188957 2313 98-99 2.018690024165899 2313 100-101 1.7968796932453373 2314 1 0.6313469735197579 2314 2 -0.12396813371121951 2314 3 -1.4957056968604476 2314 4 -0.47244340688067155 2314 5 -0.2851491801053285 2314 6 -3.5065203957567164 2314 7 -2.725655767077342 2314 8 -2.638548332490924 2314 9 -3.2503834860901044 2314 10-11 -2.5635648176080608 2314 12-13 -1.3668460700434721 2314 14-15 -1.2794524772816303 2314 16-17 -1.8959552426775304 2314 18-19 -2.959967577308767 2314 20-21 -2.955206874241931 2314 22-23 -4.101602904003464 2314 24-25 -3.2183932359192227 2314 26-27 -1.7643322926483052 2314 28-29 -1.8376663352675493 2314 30-31 -1.9519249194190849 2314 32-33 -2.264198472568978 2314 34-35 -1.636713858722345 2314 36-37 -2.233376713040734 2314 38-39 -3.5330721745546363 2314 40-41 -2.720386800600785 2314 42-43 -1.819230400011115 2314 44-45 -2.5319213580009645 2314 46-47 -2.185229569656979 2314 48-49 -2.575815594015811 2314 50-51 -3.216558100362061 2314 52-53 -2.301580889658961 2314 54-55 -2.803305944091779 2314 56-57 -1.7165364719970135 2314 58-59 -1.2837354110364707 2314 60-61 -1.4893841080214187 2314 62-63 -1.0942566599741603 2314 64-65 -1.4846935510369619 2314 66-67 -1.0925513664979967 2314 68-69 -0.9934599846263517 2314 70-71 -1.0837788722739923 2314 72-73 -2.106138895368275 2314 74-75 -2.2041791373425212 2314 76-77 -1.4857036911414916 2314 78-79 -2.374747800401053 2314 80-81 -1.2736171405037915 2314 82-83 -1.028950445723897 2314 84-85 -2.0074163772948417 2314 86-87 -0.769650331412052 2314 88-89 0.34634092694738783 2314 90-91 1.3965056814481933 2314 92-93 2.281415002051194 2314 94-95 2.2352000224440296 2314 96-97 1.2571316805560961 2314 98-99 1.0932502086999278 2314 100-101 0.9961242570867945 2315 1 -2.6423287901149024 2315 2 -1.6960614685730384 2315 3 -2.4197794869627103 2315 4 -4.172506977967885 2315 5 -3.576627527971681 2315 6 -2.591041059337627 2315 7 -5.4361569067416795 2315 8 -2.8378411599750706 2315 9 -2.4204142592604256 2315 10-11 -3.825689402051161 2315 12-13 -2.6773199649561477 2315 14-15 -1.668000897755988 2315 16-17 -2.674583298221094 2315 18-19 -1.9162398997675254 2315 20-21 -1.1529890323537941 2315 22-23 -0.8769088863851948 2315 24-25 -2.1482299609511912 2315 26-27 -3.6734481185736954 2315 28-29 -2.78876647238458 2315 30-31 -2.9627686283379475 2315 32-33 -3.559274914195978 2315 34-35 -3.5152976973738674 2315 36-37 -2.8762708202838176 2315 38-39 -1.7510276569281729 2315 40-41 -1.6343929850105354 2315 42-43 -2.8051057083755495 2315 44-45 -6.534108084298403 2315 46-47 -3.310748075989519 2315 48-49 -2.451574017929257 2315 50-51 -3.3039896348966487 2315 52-53 -3.191199299369405 2315 54-55 -1.798835273885775 2315 56-57 -1.364601275797586 2315 58-59 -2.5741987058053297 2315 60-61 -2.708091128681275 2315 62-63 -4.724084750057518 2315 64-65 -4.098001346757897 2315 66-67 -3.3929091213518063 2315 68-69 -1.9042712380486932 2315 70-71 -3.5691898288327586 2315 72-73 -2.6414317168972836 2315 74-75 -1.6731028505490784 2315 76-77 -2.956586793291443 2315 78-79 -1.5038261387391856 2315 80-81 -1.2393119784421849 2315 82-83 -0.7955477846709407 2315 84-85 -2.321404994050514 2315 86-87 -1.8467325822751768 2315 88-89 -1.386166081504438 2315 90-91 -0.3649624980217716 2315 92-93 0.1264200564737017 2315 94-95 0.015814413925806292 2315 96-97 -0.11766588200633876 2315 98-99 -0.2798937612028123 2315 100-101 -0.7713060566447094 2316 1 -7.144671083575091 2316 2 -5.7551096614581425 2316 3 -3.3640657104916407 2316 4 -3.9316545408785757 2316 5 -1.8526998567318884 2316 6 -2.7276865165501576 2316 7 -3.2794995882181617 2316 8 -2.4867908345776684 2316 9 -6.935171812171024 2316 10-11 -6.66031133853636 2316 12-13 -5.842393451456026 2316 14-15 -4.7772184928751855 2316 16-17 -4.860604932495235 2316 18-19 -4.240108737642956 2316 20-21 -3.0157919926341705 2316 22-23 -3.444768361395994 2316 24-25 -3.112209746248844 2316 26-27 -4.162936820359516 2316 28-29 -4.30586758059696 2316 30-31 -6.781809344811606 2316 32-33 -4.826043053957552 2316 34-35 -5.703252788431513 2316 36-37 -4.733585085087583 2316 38-39 -6.703786989035422 2316 40-41 -3.900267110002254 2316 42-43 -4.7367279319297175 2316 44-45 -3.3575467919202424 2316 46-47 -4.377159745500588 2316 48-49 -5.4830328329969085 2316 50-51 -5.0418297406389705 2316 52-53 -6.2582576626977655 2316 54-55 -5.43968976146768 2316 56-57 -4.625191281381234 2316 58-59 -3.3323888464060296 2316 60-61 -3.744512451280542 2316 62-63 -4.620064680466957 2316 64-65 -3.3386430563008958 2316 66-67 -4.30104311000154 2316 68-69 -3.4801877293719556 2316 70-71 -2.8202620787278683 2316 72-73 -2.3859327689577157 2316 74-75 -2.5338335514705363 2316 76-77 -3.0330504506762104 2316 78-79 -4.883007171998173 2316 80-81 -5.883174380925485 2316 82-83 -3.6697193067989105 2316 84-85 -2.2935708189636834 2316 86-87 -2.368137674436934 2316 88-89 -1.627669313396222 2316 90-91 -1.3383300693237068 2316 92-93 -1.2155352301693867 2316 94-95 -1.9362192015342785 2316 96-97 -3.66319801637985 2316 98-99 -4.945894228084203 2316 100-101 -4.392025653360136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12625.0 3 3690.0 4 3637.0 5 3894.0 6 4669.0 7 5773.0 8 7071.0 9 8980.0 10 10916.0 11 13890.0 12 18098.0 13 21412.0 14 24440.0 15 28706.0 16 33220.0 17 36143.0 18 28253.0 19 23531.0 20 24538.0 21 26316.0 22 29174.0 23 32692.0 24 36647.0 25 41466.0 26 46653.0 27 53085.0 28 60604.0 29 68456.0 30 77600.0 31 87503.0 32 98737.0 33 111455.0 34 126677.0 35 147264.0 36 163757.0 37 144137.0 38 46289.0 39 731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.635214713022165 21.16739243300888 13.786266879329457 20.411125974639493 2 15.726327850069563 21.865361907100183 40.65278898203033 21.755521260799927 3 18.44007868883127 28.556328867868825 31.17206020338666 21.831532239913244 4 11.543824916667495 18.339465724774996 39.560677932804275 30.55603142575324 5 11.771442601962521 39.79240655810541 34.19393120946126 14.242219630470812 6 27.323149461552042 38.94980137824901 19.259878172422624 14.467170987776312 7 25.992445528965046 36.01685894031523 21.69173125829085 16.29896427242887 8 26.194017063527102 33.88196118863391 20.760039837119283 19.163981910719706 9 28.246643299260903 15.878625959109574 21.561666381056376 34.31306436057315 10-11 23.90862926350276 27.91923578754435 27.871279514308224 20.300855434644667 12-13 25.70558441531861 26.687989137410646 28.149725496823415 19.45670095044733 14-15 23.10413188342656 26.83466910721961 28.089818976078877 21.97138003327495 16-17 20.07455737043464 30.199097417244975 28.46606250831461 21.26028270400578 18-19 19.724910955018395 27.898099788865427 32.89821676092665 19.478772495189524 20-21 21.511482533102924 26.671435519158127 32.744118797067685 19.07296315067127 22-23 20.9700719677023 26.990784623486046 32.40260956058744 19.63653384822421 24-25 19.984391560557174 27.535786166135363 33.068881383468394 19.410940889839065 26-27 20.052286518179123 27.557447565688854 33.085958146202074 19.304307769929956 28-29 19.524534724604024 27.62153816259091 33.28903671985382 19.56489039295125 30-31 19.926447824364335 27.602461258989923 33.032728026082694 19.438362890563045 32-33 19.709315663677245 27.617733330524167 32.77764512270204 19.895305883096544 34-35 20.112558938370544 27.624982993271917 32.812554403243055 19.449903665114483 36-37 20.215545796532165 27.240842917438197 32.48449727634293 20.059114009686716 38-39 19.64430749209455 27.980693385638418 32.35244723654552 20.02255188572151 40-41 19.803424332675874 27.72467702855444 32.71704474068508 19.754853898084598 42-43 19.76325301821682 27.703873177111145 32.225149539422965 20.30772426524907 44-45 19.889972778428696 27.77284834761698 31.993230444277692 20.34394842967663 46-47 20.11541690208348 28.01538579926775 31.26421095843195 20.60498634021682 48-49 19.76506443589794 28.22215346770126 31.118000223045648 20.894781873355146 50-51 19.72463623454862 28.448303871959013 30.657899628279996 21.169160265212376 52-53 19.882167389399104 28.901452548766144 30.214665292040692 21.00171476979406 54-55 19.94414985869153 28.538134259875857 30.21937572538591 21.2983401560467 56-57 20.028192606361685 28.7985396265021 29.85091922996786 21.322348537168356 58-59 20.008439638441676 28.288152199105234 30.046137080329906 21.657271082123184 60-61 20.04281689283812 28.502634514405944 30.085156937252304 21.369391655503637 62-63 19.84288568478264 28.116939152208698 30.210880115137208 21.82929504787145 64-65 19.86121345495829 28.13566352599849 30.14906887169675 21.85405414734647 66-67 19.81798650052893 28.259661202595044 29.625406060719534 22.29694623615649 68-69 20.03752851280542 27.978225973253185 29.58758130373258 22.39666421020882 70-71 19.891241987017732 28.150519872898176 29.9283772282827 22.02986091180139 72-73 20.181861815457378 27.68914916532041 29.8226744866662 22.306314532556016 74-75 19.862431759520742 28.27372111203043 29.39515806855408 22.468689059894746 76-77 19.887472151245483 28.107490703909377 29.567115242251603 22.437921902593537 78-79 19.979639225576364 28.053464888055256 29.29454717034703 22.672348716021347 80-81 19.850627158666487 27.871191100863555 29.421888695218396 22.85629304525156 82-83 20.232206503362924 28.02224873204183 29.130791806026185 22.614752958569056 84-85 20.094703449543445 27.760362049432054 29.053103061340664 23.09183143968384 86-87 20.005332671481096 27.51310192309831 29.406492981185796 23.075072424234797 88-89 20.50654481944927 27.23614298647249 29.167524020794268 23.089788173283978 90-91 20.29038805507924 27.201980379787855 29.502198189891583 23.00543337524132 92-93 20.888120167282125 26.97490327588774 29.41211887850841 22.724857678321726 94-95 20.707767038646224 26.61940016465149 29.463066209236306 23.209766587465978 96-97 21.079655086594347 26.50220328809639 28.865299310465986 23.552842314843282 98-99 20.97570933680421 26.48009147366559 28.670544847049896 23.87365434248031 100-101 21.35591532193688 26.448551177850003 28.911966276417957 23.283567223795163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3294.0 1 2108.0 2 961.5 3 997.5 4 913.0 5 926.5 6 1291.0 7 2212.5 8 3445.0 9 3895.0 10 3410.5 11 3040.5 12 3004.0 13 3016.0 14 3223.5 15 3581.5 16 3867.5 17 4170.0 18 4579.5 19 5062.0 20 5698.0 21 6476.5 22 7302.0 23 8293.0 24 9471.0 25 11454.0 26 15171.0 27 19123.0 28 21287.5 29 23413.0 30 27051.5 31 30897.5 32 35733.0 33 42046.0 34 49769.0 35 55018.0 36 58612.5 37 63112.0 38 63433.0 39 61087.5 40 59305.0 41 58273.0 42 58174.5 43 58587.5 44 59119.0 45 59459.0 46 58933.5 47 57714.5 48 57972.0 49 56992.5 50 52638.5 51 47631.5 52 43536.5 53 40399.0 54 37547.0 55 34791.5 56 32054.0 57 29187.0 58 26399.0 59 23688.5 60 20588.0 61 17051.5 62 13427.0 63 9699.0 64 6768.5 65 5220.0 66 4113.0 67 3175.0 68 2351.5 69 1686.5 70 1199.5 71 902.5 72 778.0 73 745.5 74 697.0 75 593.0 76 435.0 77 311.5 78 231.0 79 166.0 80 118.5 81 85.5 82 60.0 83 45.5 84 31.0 85 15.5 86 7.5 87 6.0 88 2.0 89 1.5 90 3.5 91 3.5 92 2.0 93 1.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1373830886263968 2 0.12080136437229708 3 0.12809965849822125 4 0.14082788345383304 5 0.1269319314380734 6 0.14263786039706222 7 0.1759180816112765 8 0.16149665241845032 9 0.17165587784173678 10-11 0.16570046983498266 12-13 0.15411077876301504 14-15 0.15962828912221375 16-17 0.15393561970399286 18-19 0.17042976442858151 20-21 0.17653113831785414 22-23 0.2118840750638309 24-25 0.2357640934438548 26-27 0.24860909110548138 28-29 0.24352947839383815 30-31 0.23412927555964777 32-33 0.2357057070908474 34-35 0.22458310684293895 36-37 0.2333702529705517 38-39 0.22075880072095466 40-41 0.22598437931511642 42-43 0.21786867624708872 44-45 0.21057038212116452 46-47 0.22656824284519034 48-49 0.2661541901842031 50-51 0.2679057807744249 52-53 0.27021204171821694 54-55 0.26778900806841016 56-57 0.24084370615549805 58-59 0.24084370615549805 60-61 0.2096362004730462 62-63 0.20225032681761096 64-65 0.2035056334072699 66-67 0.2116505296518013 68-69 0.21185488188732718 70-71 0.2163506310688965 72-73 0.25041906804871056 74-75 0.25342596522859134 76-77 0.26965737136464674 78-79 0.2652200085360848 80-81 0.27955385819939993 82-83 0.27829855160974093 84-85 0.2536011242876135 86-87 0.2564328624084721 88-89 0.2609578047665451 90-91 0.45561790569319494 92-93 0.6034521515079151 94-95 0.3558064352270558 96-97 0.2950846280993666 98-99 0.325270372604189 100-101 0.34515092580320644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1712729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.57863485450733 #Duplication Level Percentage of deduplicated Percentage of total 1 76.30887873053508 35.543633985464744 2 11.395666462369212 10.615891741489015 3 4.667036074023098 6.521525074342059 4 2.331899250278195 4.344667347848297 5 1.3202851707450878 3.074854043597815 6 0.8639496760832878 2.4144957896972 7 0.5833704034133186 1.9020817903860923 8 0.40699967949933524 1.516599156584084 9 0.3024744538934741 1.2679962426648577 >10 1.5506522866659922 13.176190557802464 >50 0.14839142545552073 4.795069046611918 >100 0.11259545618847655 9.97775769620045 >500 0.005850698137573341 1.8262913818608215 >1k 0.0015601861700195578 1.0322964119507763 >5k 1.300155141682965E-4 0.4379053945099181 >10k+ 2.60031028336593E-4 1.5527443389896696 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14966 0.8738101591086506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10674 0.6232159320009178 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7231 0.42219171859646215 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2236 0.13055188532453177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2190 0.1278661130861917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1830 0.10684702600353004 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 5.8386353007393465E-5 0.0 5.8386353007393465E-5 0.0 5 0.0 5.8386353007393465E-5 0.0 5.8386353007393465E-5 0.0 6 0.0 5.8386353007393465E-5 0.0 5.8386353007393465E-5 0.0 7 0.0 5.8386353007393465E-5 0.0 5.8386353007393465E-5 0.0 8 0.0 5.8386353007393465E-5 0.0 5.8386353007393465E-5 0.0 9 0.0 5.8386353007393465E-5 0.0 1.1677270601478693E-4 0.0 10-11 0.0 5.8386353007393465E-5 0.0 1.1677270601478693E-4 0.0 12-13 0.0 5.8386353007393465E-5 0.0 1.1677270601478693E-4 0.0 14-15 0.0 5.8386353007393465E-5 0.0 1.1677270601478693E-4 0.0 16-17 0.0 5.8386353007393465E-5 0.0 1.1677270601478693E-4 0.0 18-19 0.0 5.8386353007393465E-5 0.0 3.21124941540664E-4 0.0 20-21 0.0 5.8386353007393465E-5 0.0 6.422498830813281E-4 0.0 22-23 0.0 5.8386353007393465E-5 0.0 0.002306260943792042 0.0 24-25 0.0 5.8386353007393465E-5 0.0 0.004992033182132141 0.0 26-27 0.0 5.8386353007393465E-5 0.0 0.010567929894338217 0.0 28-29 0.0 5.8386353007393465E-5 0.0 0.02849254026760801 0.0 30-31 0.0 5.8386353007393465E-5 0.0 0.07937624691355141 0.0 32-33 0.0 5.8386353007393465E-5 0.0 0.18234058044208978 0.0 34-35 0.0 5.8386353007393465E-5 0.0 0.3358383024985272 0.0 36-37 0.0 5.8386353007393465E-5 0.0 0.5785211787737581 0.0 38-39 0.0 5.8386353007393465E-5 0.0 0.9420346125977899 0.0 40-41 0.0 5.8386353007393465E-5 0.0 1.3449004483488047 0.0 42-43 0.0 5.8386353007393465E-5 0.0 1.733490820789512 0.0 44-45 0.0 5.8386353007393465E-5 0.0 2.1786575692943835 0.0 46-47 0.0 5.8386353007393465E-5 0.0 2.696690486352482 0.0 48-49 0.0 5.8386353007393465E-5 0.0 3.2493173175674612 0.0 50-51 0.0 5.8386353007393465E-5 0.0 3.826699962457575 0.0 52-53 0.0 5.8386353007393465E-5 0.0 4.374947817197 0.0 54-55 0.0 5.8386353007393465E-5 0.0 4.929968488885282 0.0 56-57 0.0 5.8386353007393465E-5 0.0 5.553388773121726 0.0 58-59 0.0 5.8386353007393465E-5 0.0 6.236742648720258 0.0 60-61 0.0 5.8386353007393465E-5 0.0 6.947830041997304 0.0 62-63 0.0 5.8386353007393465E-5 0.0 7.624381907470475 0.0 64-65 0.0 5.8386353007393465E-5 0.0 8.292059047286523 0.0 66-67 0.0 5.8386353007393465E-5 0.0 9.008663950922767 0.0 68-69 0.0 5.8386353007393465E-5 0.0 9.782662639565277 0.0 70-71 0.0 5.8386353007393465E-5 0.0 10.62789267887681 0.0 72-73 0.0 5.8386353007393465E-5 0.0 11.428690703549716 0.0 74-75 0.0 5.8386353007393465E-5 0.0 12.214921333147275 0.0 76-77 0.0 5.8386353007393465E-5 0.0 13.013091971934848 0.0 78-79 0.0 5.8386353007393465E-5 0.0 13.867109157373992 0.0 80-81 0.0 5.8386353007393465E-5 0.0 14.759427790385987 0.0 82-83 0.0 5.8386353007393465E-5 0.0 15.575756584958858 0.0 84-85 0.0 5.8386353007393465E-5 0.0 16.334574821819448 0.0 86-87 0.0 5.8386353007393465E-5 0.0 17.113098452820033 0.0 88-89 0.0 5.8386353007393465E-5 0.0 17.929923531393467 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7715 0.0 41.618225 1 GTATCAA 13630 0.0 38.798042 1 TATCAAC 17445 0.0 31.075548 2 ATCAACG 17680 0.0 30.717945 3 TCAACGC 17755 0.0 30.669228 4 CAACGCA 17850 0.0 30.373137 5 CATTCCG 485 0.0 30.317186 9 AACGCAG 18295 0.0 29.764858 6 GTACATG 12880 0.0 29.23718 1 TACATGG 13210 0.0 29.082098 2 ACATGGG 13730 0.0 27.049557 3 ACGCAGA 20390 0.0 26.520517 7 CGCAGAG 20480 0.0 26.239536 8 CATGGGA 8980 0.0 24.720152 4 GCAGAGT 20725 0.0 24.671364 9 CATGGGG 4160 0.0 23.37449 4 GAGTACT 11465 0.0 23.330454 12-13 TAGGCAT 685 0.0 22.850988 5 TTAGGCA 755 0.0 22.617115 4 AGTACTT 11640 0.0 22.612997 12-13 >>END_MODULE