FastQCFastQC Report
Fri 27 May 2016
SRR939843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712729
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156830.9156731742149516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124050.7242827090567159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87920.5133328156410034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26930.1572344486489106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.1463745869895354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.13382152109294582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.047.0061381
GTATCAA134550.038.0620541
CATTCCG5250.031.40229
TTAGGCA7250.029.9089344
ATCAACG177850.028.7587283
TCAACGC177800.028.712954
GTACATG133950.028.6216011
TATCAAC180100.028.4542872
CAACGCA181250.028.1404085
TACATGG135850.028.0750642
AACGCAG184600.027.6342756
ACATGGG138200.026.6401583
CATGGGG41100.024.429744
ACGCAGA208300.024.422267
CGAAACG500.001415865124.3933966-67
CGCAGAG208800.024.29618
CATGGGT13050.023.8404564
ATGGGAT34950.023.0637575
GCAGAGT213750.023.0503189
GAGTACT119050.023.02740112-13
Produced by FastQC (version 0.11.5)