##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939843_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1712729 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38901659281766 31.0 31.0 34.0 30.0 34.0 2 31.574657753795258 31.0 31.0 34.0 30.0 34.0 3 31.61010352484252 31.0 31.0 34.0 30.0 34.0 4 35.45083839883601 37.0 35.0 37.0 33.0 37.0 5 35.20811465211367 37.0 35.0 37.0 33.0 37.0 6 35.25046869644877 37.0 35.0 37.0 32.0 37.0 7 35.16137696039478 37.0 35.0 37.0 32.0 37.0 8 35.11082955914216 37.0 35.0 37.0 32.0 37.0 9 36.66234004328764 39.0 35.0 39.0 32.0 39.0 10-11 36.53320052384236 38.0 35.0 39.0 32.0 39.0 12-13 36.480273586772924 38.0 35.0 39.0 32.0 39.0 14-15 37.40663117165646 39.0 36.0 41.0 32.0 41.0 16-17 37.361710755174926 39.0 36.0 41.0 32.0 41.0 18-19 37.45707172588308 39.0 36.0 41.0 32.0 41.0 20-21 37.43631800477483 39.0 36.0 41.0 32.0 41.0 22-23 37.28087368170913 39.0 36.0 40.5 31.0 41.0 24-25 37.10904235287661 39.0 36.0 40.0 31.0 41.0 26-27 36.79577008388367 39.0 35.5 40.0 30.5 41.0 28-29 36.551962686449514 38.0 35.0 40.0 30.0 41.0 30-31 36.215795960715326 38.0 35.0 40.0 30.0 41.0 32-33 36.01102042413015 38.0 35.0 40.0 30.0 41.0 34-35 35.93704403907448 38.0 35.0 40.0 30.0 41.0 36-37 35.801334011393514 38.0 35.0 40.0 29.0 41.0 38-39 35.59435730929995 38.0 34.5 40.0 28.5 41.0 40-41 35.352643938416406 38.0 34.0 40.0 27.0 41.0 42-43 35.01048910831778 38.0 34.0 40.0 26.5 41.0 44-45 34.571927607928636 38.0 33.5 40.0 24.5 41.0 46-47 34.02593317448353 38.0 33.0 40.0 22.5 41.0 48-49 33.55253925168547 38.0 33.0 40.0 19.5 41.0 50-51 33.2262509130166 38.0 33.0 40.0 16.0 41.0 52-53 32.94112699674029 37.0 32.5 40.0 13.0 41.0 54-55 32.610968226730556 37.0 32.0 40.0 8.5 41.0 56-57 31.437442525933754 36.5 29.5 39.5 4.0 41.0 58-59 28.443275030667433 34.0 22.0 38.5 2.0 40.0 60-61 28.41249286956664 34.0 22.0 38.0 2.0 40.0 62-63 30.033803654868926 35.0 28.5 38.5 2.0 40.0 64-65 29.561834067152482 35.0 28.0 38.0 2.0 40.0 66-67 24.368410589182524 28.0 16.5 36.0 2.0 39.0 68-69 16.93650250565034 13.0 7.0 29.0 2.0 35.5 70-71 15.893331344304908 12.5 6.5 25.5 2.0 33.5 72-73 17.97193397204111 16.5 7.0 30.0 2.0 35.0 74-75 18.660979933194334 17.5 7.5 30.5 2.0 35.0 76-77 19.588867240526668 22.5 7.5 31.0 2.0 34.5 78-79 22.610755408473846 27.5 9.0 34.0 2.0 36.0 80-81 25.03082653472908 31.5 16.5 35.0 2.0 36.0 82-83 25.81133384207309 32.5 21.0 35.0 2.0 36.0 84-85 25.733968421157115 33.0 20.5 35.0 2.0 36.0 86-87 25.432913496530976 33.0 19.5 35.0 2.0 35.5 88-89 24.99478317935879 32.0 15.0 35.0 2.0 35.0 90-91 23.544644540963574 31.5 2.0 34.5 2.0 35.0 92-93 21.68284941750855 30.5 2.0 34.0 2.0 35.0 94-95 17.46690661511541 19.5 2.0 33.0 2.0 35.0 96-97 16.233551250664874 12.0 2.0 32.5 2.0 34.5 98-99 15.17902715490892 2.0 2.0 32.0 2.0 34.0 100-101 14.579855598871742 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4789151775723539 1101 2 0.4609278670721473 1101 3 0.3205735387454922 1101 4 0.26612190084461673 1101 5 0.34075047081811505 1101 6 0.4650191301645279 1101 7 0.3941701746742652 1101 8 0.43294999218279173 1101 9 0.6035381441838155 1101 10-11 0.5837292514035326 1101 12-13 0.5256058833428909 1101 14-15 0.5037315542274428 1101 16-17 0.3996971006105312 1101 18-19 0.6140468372485373 1101 20-21 0.6433207302215678 1101 22-23 0.53543177020358 1101 24-25 0.47697484101702514 1101 26-27 0.6722240519646405 1101 28-29 0.49605508641916884 1101 30-31 0.675305096925527 1101 32-33 0.7069779370044458 1101 34-35 0.9600836772105552 1101 36-37 0.8543952762865885 1101 38-39 0.8805041148011057 1101 40-41 0.9544967651514327 1101 42-43 1.0313933476880877 1101 44-45 0.5696539573354258 1101 46-47 0.6617702255887465 1101 48-49 0.7336470688987973 1101 50-51 0.4618524667243662 1101 52-53 0.5324065702398357 1101 54-55 0.5449797579792914 1101 56-57 1.0626025571017585 1101 58-59 -8.02372350241087 1101 60-61 -9.103412143342204 1101 62-63 -2.055825769839153 1101 64-65 0.13157411266544372 1101 66-67 -1.029410582609568 1101 68-69 -3.3946269584339994 1101 70-71 -4.2200493042885 1101 72-73 -6.439482311781223 1101 74-75 -7.149544208422958 1101 76-77 -8.055249271004033 1101 78-79 -10.944488648167221 1101 80-81 -7.142059375867408 1101 82-83 -3.251062183409065 1101 84-85 -2.447206346866608 1101 86-87 -2.220338200768051 1101 88-89 -2.4611315311274566 1101 90-91 -7.640268673748235 1101 92-93 -13.354616432801997 1101 94-95 -11.545427010862902 1101 96-97 -11.663923159518527 1101 98-99 -11.128985501402148 1101 100-101 -10.991216554484325 1102 1 0.5770355748428102 1102 2 0.6006481562417996 1102 3 0.5414194275367876 1102 4 0.38873118400115914 1102 5 0.625000183675354 1102 6 0.5903633636622629 1102 7 0.5557106111312606 1102 8 0.6923040898788955 1102 9 0.8176966469876774 1102 10-11 0.7839184278105407 1102 12-13 0.8137499276642188 1102 14-15 0.9891724028187667 1102 16-17 0.9890734940974539 1102 18-19 1.0456521341879252 1102 20-21 1.0675001775562052 1102 22-23 1.1339886244701844 1102 24-25 1.0966809419561514 1102 26-27 1.031923396603517 1102 28-29 0.9847862532322225 1102 30-31 0.9497183122488124 1102 32-33 0.8743166303359757 1102 34-35 0.9375149318555103 1102 36-37 0.9533291321071431 1102 38-39 1.0475205326107115 1102 40-41 0.8786978326468713 1102 42-43 1.0217750786522117 1102 44-45 0.8650995353369169 1102 46-47 0.7872583682818117 1102 48-49 0.9764725536633492 1102 50-51 0.898372972095622 1102 52-53 0.9521538846104889 1102 54-55 0.7005820821465747 1102 56-57 1.281718833029064 1102 58-59 -9.082234413835774 1102 60-61 -9.508894880995065 1102 62-63 -2.502339518910162 1102 64-65 0.4330821188812415 1102 66-67 -0.76049767131979 1102 68-69 -3.522125776081456 1102 70-71 -4.086714394485108 1102 72-73 -6.726015654122957 1102 74-75 -7.195841080255942 1102 76-77 -8.330176640776346 1102 78-79 -11.29436703476272 1102 80-81 -7.080432458767213 1102 82-83 -3.010450738236443 1102 84-85 -2.184762593066189 1102 86-87 -2.0932724619666168 1102 88-89 -2.157708113790793 1102 90-91 -7.599803840291035 1102 92-93 -13.120380908175283 1102 94-95 -11.57426830537003 1102 96-97 -11.51704862401417 1102 98-99 -11.11502090441546 1102 100-101 -10.85111183183045 1103 1 0.230839009071655 1103 2 0.17666785407091368 1103 3 0.3190933304914658 1103 4 0.22766430419916617 1103 5 0.19445875119971845 1103 6 0.1891381793173963 1103 7 0.23647016354708228 1103 8 0.2540351268509937 1103 9 0.31798386402475387 1103 10-11 0.2318313345294598 1103 12-13 0.25458502280164197 1103 14-15 0.3734512663262137 1103 16-17 0.35468325019501634 1103 18-19 0.4013258308826195 1103 20-21 0.39032078292927963 1103 22-23 0.4824290411153598 1103 24-25 0.6439023620561954 1103 26-27 0.4079995581050895 1103 28-29 0.3891316355866792 1103 30-31 0.4085052456784055 1103 32-33 0.47493076945834645 1103 34-35 0.5259924752990273 1103 36-37 0.3345164161787153 1103 38-39 0.36680816216777146 1103 40-41 0.24085556997477653 1103 42-43 0.20702130151613574 1103 44-45 0.21242743715716017 1103 46-47 0.16213332316243623 1103 48-49 0.2550032090544718 1103 50-51 0.06576352538804997 1103 52-53 -0.009274981475208222 1103 54-55 0.002107890427915038 1103 56-57 0.6724644628955119 1103 58-59 -6.454637600552633 1103 60-61 -8.088716208278107 1103 62-63 -2.251312086331396 1103 64-65 -0.3236682549649892 1103 66-67 -1.653461111736437 1103 68-69 -3.664233495361609 1103 70-71 -4.438932720685283 1103 72-73 -7.149157251606585 1103 74-75 -7.802460994658045 1103 76-77 -8.364339740864006 1103 78-79 -11.208065276396301 1103 80-81 -7.691742983183239 1103 82-83 -3.998718154314407 1103 84-85 -3.0887931711098133 1103 86-87 -2.829013450983272 1103 88-89 -3.101796702456806 1103 90-91 -8.301322945122674 1103 92-93 -13.306063113094584 1103 94-95 -11.73895582148409 1103 96-97 -11.776737429253174 1103 98-99 -11.402291826913267 1103 100-101 -11.164597110023612 1104 1 0.25775761644305106 1104 2 0.2500964572096258 1104 3 0.30356596984626805 1104 4 0.15297405710410317 1104 5 0.13453610373861835 1104 6 0.229940582697445 1104 7 0.11630360913535043 1104 8 0.25239346637739146 1104 9 0.06948380037130875 1104 10-11 0.19670102449909166 1104 12-13 0.29302939240945847 1104 14-15 0.30882669653102823 1104 16-17 0.36098793367417414 1104 18-19 0.31020129753854775 1104 20-21 0.2543543005493092 1104 22-23 0.3198136624426411 1104 24-25 0.3062166109618403 1104 26-27 0.2706279394904314 1104 28-29 0.18548821022605466 1104 30-31 0.3169475152665271 1104 32-33 0.1888398092270549 1104 34-35 0.2683463090318696 1104 36-37 0.09184186700288421 1104 38-39 0.2002152591462405 1104 40-41 -0.0017585900336669624 1104 42-43 0.12571517631361218 1104 44-45 0.13360787672501573 1104 46-47 0.24999765753779712 1104 48-49 0.41595788562423053 1104 50-51 0.161145903067343 1104 52-53 0.33242025670292463 1104 54-55 0.14991037759113368 1104 56-57 0.9703971403988625 1104 58-59 -5.714218396362167 1104 60-61 -8.079022794832106 1104 62-63 -2.0493713906699504 1104 64-65 0.15381746653322637 1104 66-67 -1.2993782055703207 1104 68-69 -3.571870576051328 1104 70-71 -4.2984139287732965 1104 72-73 -7.3220923773194215 1104 74-75 -8.0583353373138 1104 76-77 -8.596773429749897 1104 78-79 -11.1086296158857 1104 80-81 -7.58486136553698 1104 82-83 -4.060568358712693 1104 84-85 -3.159912160619509 1104 86-87 -2.9508424756492566 1104 88-89 -3.1009547904160755 1104 90-91 -8.097273933056812 1104 92-93 -13.789543003501848 1104 94-95 -11.710976187322755 1104 96-97 -11.508843058067619 1104 98-99 -10.960886864773467 1104 100-101 -10.698226803649236 1105 1 0.08971850167207762 1105 2 0.11324133855344343 1105 3 0.06598481799781553 1105 4 0.013010694748786023 1105 5 0.0532002271001204 1105 6 0.0719447489123155 1105 7 0.14163230180216857 1105 8 0.18530908372704857 1105 9 0.14729641538892224 1105 10-11 0.10677252059479514 1105 12-13 0.14100512054255887 1105 14-15 0.23759499817231244 1105 16-17 0.20334793862231493 1105 18-19 0.30816534255443884 1105 20-21 0.0769805278493223 1105 22-23 0.14619605010526726 1105 24-25 -0.0022526181509618937 1105 26-27 0.10563006230518823 1105 28-29 0.06970374617569775 1105 30-31 0.2442355735935351 1105 32-33 0.21095736276704002 1105 34-35 0.23331394756080215 1105 36-37 0.019366260282581038 1105 38-39 0.3272632408686533 1105 40-41 0.19332293216196916 1105 42-43 0.0510592937495602 1105 44-45 0.5223149221792127 1105 46-47 0.391101460125185 1105 48-49 0.24807492755059002 1105 50-51 0.17691715679563202 1105 52-53 0.43811791442883674 1105 54-55 0.5197222717680603 1105 56-57 0.9644372234177148 1105 58-59 -6.37904522660266 1105 60-61 -8.146381310755508 1105 62-63 -2.4858236326830507 1105 64-65 -0.0785278543401553 1105 66-67 -1.5714776235525463 1105 68-69 -3.900202058921437 1105 70-71 -4.828496619280649 1105 72-73 -6.008970709489535 1105 74-75 -6.278414721422621 1105 76-77 -8.233084290772727 1105 78-79 -11.304012761041943 1105 80-81 -7.464362038270721 1105 82-83 -3.7245840522698757 1105 84-85 -2.893839093030003 1105 86-87 -2.6558330757033204 1105 88-89 -2.676931896087961 1105 90-91 -7.635757013917727 1105 92-93 -10.719182384617348 1105 94-95 -11.306874153238597 1105 96-97 -11.756238028371122 1105 98-99 -11.066682263610652 1105 100-101 -10.617005658276131 1106 1 0.017101255076010347 1106 2 0.021259941784769865 1106 3 0.11891693685378968 1106 4 -0.01926999676707908 1106 5 0.02615955048838714 1106 6 -0.06266493787642702 1106 7 0.03534383499754057 1106 8 0.05180373933832527 1106 9 0.1397231404750059 1106 10-11 0.1890510797422067 1106 12-13 0.16772874763396572 1106 14-15 0.16850120987385253 1106 16-17 -0.012563345871548393 1106 18-19 0.13517134012129617 1106 20-21 0.1540364677299948 1106 22-23 0.13721765660921648 1106 24-25 0.21458175742422014 1106 26-27 0.20167137638286192 1106 28-29 0.31972001951390894 1106 30-31 0.3959070137727494 1106 32-33 0.3507739566339012 1106 34-35 0.18505339907074614 1106 36-37 0.25076022321078995 1106 38-39 0.22689546342507327 1106 40-41 0.25121729687328553 1106 42-43 0.2400275416609574 1106 44-45 0.20823668894748693 1106 46-47 0.053324196111034894 1106 48-49 0.05527739481312466 1106 50-51 -0.06976252105960867 1106 52-53 0.07401543953466216 1106 54-55 -0.06693830455501626 1106 56-57 0.6462205119339011 1106 58-59 -6.216832030159896 1106 60-61 -8.198710754948056 1106 62-63 -2.3338272868892 1106 64-65 -0.2525150915537999 1106 66-67 -1.6034160138163749 1106 68-69 -3.9511926001838873 1106 70-71 -4.428601778714473 1106 72-73 -5.683665556340586 1106 74-75 -6.022665182687588 1106 76-77 -8.322734110980399 1106 78-79 -11.233168186011753 1106 80-81 -7.508152469098935 1106 82-83 -3.7259700917658307 1106 84-85 -2.8334537852731216 1106 86-87 -2.654117080246383 1106 88-89 -2.61696127788068 1106 90-91 -8.108979516465345 1106 92-93 -11.296395446384206 1106 94-95 -11.624990418945174 1106 96-97 -11.915433923449616 1106 98-99 -11.139787149203475 1106 100-101 -10.744886600289178 1107 1 0.05166675129155962 1107 2 0.10035116697053681 1107 3 0.02146991571290613 1107 4 -0.01658046911537525 1107 5 -0.03851691176957672 1107 6 -0.06897657768235632 1107 7 0.10061093410708821 1107 8 0.13213035115520455 1107 9 0.1767893447639679 1107 10-11 0.14336825518552843 1107 12-13 0.15242572675097676 1107 14-15 0.15927783401005513 1107 16-17 -0.07758463412766758 1107 18-19 -0.18635328586626798 1107 20-21 0.005165470647490622 1107 22-23 -0.027921759017210945 1107 24-25 -0.025631861993801408 1107 26-27 -0.058757451300849084 1107 28-29 0.12012909022223539 1107 30-31 0.1220990524945762 1107 32-33 0.03549419279653421 1107 34-35 0.18457213941389483 1107 36-37 0.23610598179344322 1107 38-39 0.354621546086598 1107 40-41 0.18481209926898856 1107 42-43 0.35789528505975454 1107 44-45 0.301462901710309 1107 46-47 0.47986704779326317 1107 48-49 0.22469173174554413 1107 50-51 0.22554266434049453 1107 52-53 0.23373089764089627 1107 54-55 0.14996991301118356 1107 56-57 0.7866124737462918 1107 58-59 -6.190450250788665 1107 60-61 -8.39626726841757 1107 62-63 -2.4313238156024433 1107 64-65 -0.28603805040264163 1107 66-67 -1.6379676939652192 1107 68-69 -3.8632896027882175 1107 70-71 -4.509696909241761 1107 72-73 -5.944961342209975 1107 74-75 -6.599880876818293 1107 76-77 -8.516791705557132 1107 78-79 -11.537699625622876 1107 80-81 -7.847580990526502 1107 82-83 -4.092084921701563 1107 84-85 -3.1163774722286774 1107 86-87 -2.8954089285367637 1107 88-89 -3.1571286134752405 1107 90-91 -7.96872168563387 1107 92-93 -11.159779876137566 1107 94-95 -11.35003917814725 1107 96-97 -11.827381251997984 1107 98-99 -11.223979957019683 1107 100-101 -10.859892690871916 1108 1 -0.09875501857714397 1108 2 -0.10849248656649735 1108 3 -0.12073694355963127 1108 4 -0.12717236942690135 1108 5 -0.26986444160641554 1108 6 -0.13371434066618093 1108 7 -0.12039505459829769 1108 8 -0.12990040319407115 1108 9 -0.05561794410215981 1108 10-11 0.019557816914705484 1108 12-13 -0.1674650256068091 1108 14-15 -0.026969481029979647 1108 16-17 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54-55 0.1723584469570696 2311 56-57 1.1739079940245425 2311 58-59 4.341763152107301 2311 60-61 4.05710035024034 2311 62-63 1.7863930328756403 2311 64-65 1.5702042191246441 2311 66-67 -0.2898096776781429 2311 68-69 -3.1701916943170136 2311 70-71 -0.4339557138433108 2311 72-73 -0.7468663321816713 2311 74-75 1.495043128178807 2311 76-77 4.336230889897738 2311 78-79 5.107658448323068 2311 80-81 3.169662419778316 2311 82-83 1.746603556135426 2311 84-85 1.4067465222286302 2311 86-87 1.4011850910610484 2311 88-89 1.4394085222431876 2311 90-91 2.6768642338235153 2311 92-93 4.397898362601129 2311 94-95 8.234380296289103 2311 96-97 8.960382204061931 2311 98-99 9.446264426665955 2311 100-101 8.61024056000304 2312 1 -0.30722608662414075 2312 2 -0.3075758146566372 2312 3 -0.32401160150162056 2312 4 -0.23419943453340863 2312 5 -0.2915005552315719 2312 6 -0.30549648400923246 2312 7 -0.4882468985629487 2312 8 -0.4199365032675857 2312 9 -0.43480692123806364 2312 10-11 -0.3830240132347953 2312 12-13 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5.4026952441310065 2312 78-79 5.421542831223565 2312 80-81 3.065354003447485 2312 82-83 1.6689098862346405 2312 84-85 1.3052302073278952 2312 86-87 0.9913393277038445 2312 88-89 1.2521121486790499 2312 90-91 2.519162447870034 2312 92-93 4.352275493790085 2312 94-95 8.325221154154292 2312 96-97 8.742156893157523 2312 98-99 8.87375983600717 2312 100-101 8.323462949339424 2313 1 -0.2565972878016076 2313 2 -0.2960527423461343 2313 3 -0.3875151039137883 2313 4 -0.39628807145455625 2313 5 -0.2599420499575089 2313 6 -0.25229388922605267 2313 7 -0.20530350953626453 2313 8 -0.3118422515845225 2313 9 -0.3947167034616186 2313 10-11 -0.47701867103413775 2313 12-13 -0.39967112496736235 2313 14-15 -0.497348778182527 2313 16-17 -0.6482114866470923 2313 18-19 -0.5329614730007748 2313 20-21 -0.46587594567871093 2313 22-23 -0.3000806951703865 2313 24-25 -0.463620726850003 2313 26-27 -0.5732884134093226 2313 28-29 -0.5057593180129629 2313 30-31 -0.5698195568140889 2313 32-33 -0.4551827278154974 2313 34-35 -0.5535312812382003 2313 36-37 -0.6222806239904202 2313 38-39 -0.9015872979412691 2313 40-41 -1.0639658387266806 2313 42-43 -0.7993545747495858 2313 44-45 -0.827063494188117 2313 46-47 -0.6688391926938024 2313 48-49 -0.7135274463366486 2313 50-51 -0.6477181703947466 2313 52-53 -0.8117485544139029 2313 54-55 -0.5597709345414827 2313 56-57 0.3231565737222617 2313 58-59 3.6312764975891305 2313 60-61 3.3563939508406193 2313 62-63 0.7378722911026756 2313 64-65 0.8029730046322037 2313 66-67 1.5469606085261667 2313 68-69 0.28639797231392095 2313 70-71 2.767983936708731 2313 72-73 7.762719904285259 2313 74-75 9.261328367754327 2313 76-77 8.202936324563833 2313 78-79 5.926647085904801 2313 80-81 3.1850874385370247 2313 82-83 1.6324696725466126 2313 84-85 1.132946007704028 2313 86-87 1.235174264605913 2313 88-89 1.3751288566841744 2313 90-91 2.8677507168334806 2313 92-93 4.498707666920996 2313 94-95 8.06637354315372 2313 96-97 8.877309527462083 2313 98-99 9.264407850398406 2313 100-101 8.5038111463467 2314 1 -0.4289880723525066 2314 2 -0.4416143615196688 2314 3 -0.4201720000290088 2314 4 -0.283579697098034 2314 5 -0.5432088085161197 2314 6 -0.4769401521307799 2314 7 -0.5204265791022138 2314 8 -0.44542322432636894 2314 9 -0.44185361307932425 2314 10-11 -0.4092285784858234 2314 12-13 -0.4495958767184618 2314 14-15 -0.7654502718954248 2314 16-17 -0.6617518112694398 2314 18-19 -0.8206100707109769 2314 20-21 -0.6685882582613445 2314 22-23 -0.742074000185994 2314 24-25 -0.7815271377195074 2314 26-27 -0.8971253708483502 2314 28-29 -0.929170806381137 2314 30-31 -0.8448579545186661 2314 32-33 -0.7989516102031828 2314 34-35 -1.0877169123389336 2314 36-37 -1.0146483417883871 2314 38-39 -0.8131104068894857 2314 40-41 -0.8879275535428519 2314 42-43 -0.8894246189139778 2314 44-45 -0.8644573730595297 2314 46-47 -0.9181176534099933 2314 48-49 -1.0348903538727328 2314 50-51 -0.8928869515137663 2314 52-53 -0.945024877235916 2314 54-55 -0.9472196672765811 2314 56-57 0.4856916661299202 2314 58-59 3.4371333430700126 2314 60-61 3.094849040997868 2314 62-63 0.7774521667672509 2314 64-65 0.6491747261996181 2314 66-67 1.2769352913583134 2314 68-69 -0.25340342856936715 2314 70-71 2.0927365317184368 2314 72-73 7.372963371049444 2314 74-75 8.685533689985615 2314 76-77 7.655147497873912 2314 78-79 5.5424008342521525 2314 80-81 2.6371564137543295 2314 82-83 1.351636315771458 2314 84-85 0.8837219788987198 2314 86-87 0.9877563344250326 2314 88-89 1.025519584974706 2314 90-91 2.207660278283875 2314 92-93 4.129275334084628 2314 94-95 7.76009938635606 2314 96-97 8.499219707776577 2314 98-99 8.741527178649552 2314 100-101 7.913637161143177 2315 1 -0.7072174314755557 2315 2 -0.5231646027771539 2315 3 -0.5284739076082587 2315 4 -0.41367115311413727 2315 5 -0.8586006044216674 2315 6 -0.7829418880532089 2315 7 -0.7797632661701854 2315 8 -0.8833464298575038 2315 9 -1.126813170255339 2315 10-11 -1.0313611428124645 2315 12-13 -0.7892584288817446 2315 14-15 -0.8772139005985906 2315 16-17 -0.9345925158216986 2315 18-19 -0.9710174765193216 2315 20-21 -0.8962351305489662 2315 22-23 -0.8434731596184051 2315 24-25 -0.8924134258677441 2315 26-27 -1.0022460442552372 2315 28-29 -0.9821843290084189 2315 30-31 -0.9597565162042017 2315 32-33 -0.919434303830748 2315 34-35 -1.0170974738785716 2315 36-37 -1.2965981727369353 2315 38-39 -1.2555258699981593 2315 40-41 -0.9323627013568014 2315 42-43 -1.009616999613968 2315 44-45 -0.8406590080423868 2315 46-47 -1.0398698333623244 2315 48-49 -0.921689006225229 2315 50-51 -0.8722812843076682 2315 52-53 -1.0530856017676626 2315 54-55 -1.0243706999717723 2315 56-57 0.052161118510415605 2315 58-59 3.1987072262212912 2315 60-61 2.9811791729488135 2315 62-63 0.7361753258483148 2315 64-65 0.5033871667785164 2315 66-67 1.6959314339183358 2315 68-69 5.827696902089617 2315 70-71 4.664915471674277 2315 72-73 8.329675629252275 2315 74-75 9.242324555996522 2315 76-77 8.115675658972279 2315 78-79 5.3203826085554375 2315 80-81 2.5415894177189635 2315 82-83 1.090319694537527 2315 84-85 0.6653481481526455 2315 86-87 0.8901163552084626 2315 88-89 0.8935806965903481 2315 90-91 1.9570492067909662 2315 92-93 3.890123881552281 2315 94-95 7.47206772289276 2315 96-97 8.084971893684044 2315 98-99 8.241806325695283 2315 100-101 7.482677148399185 2316 1 -0.4918217021784308 2316 2 -0.5378486534148585 2316 3 -0.5897360015712962 2316 4 -0.4983975069913029 2316 5 -0.6362637999724114 2316 6 -0.5840188698542974 2316 7 -0.5715269106210812 2316 8 -0.5874821156841818 2316 9 -0.5398505176119386 2316 10-11 -0.6713615213649433 2316 12-13 -0.7027393135588866 2316 14-15 -0.7818764052910652 2316 16-17 -0.8168909287404276 2316 18-19 -0.8953629750571608 2316 20-21 -0.8117250247750931 2316 22-23 -0.8336927831479741 2316 24-25 -2.181053223720724 2316 26-27 -1.316141128435845 2316 28-29 -1.143010614430679 2316 30-31 -0.6502463315652349 2316 32-33 -0.6729195103153458 2316 34-35 -0.6574738812262737 2316 36-37 -0.5399023680447783 2316 38-39 -0.9572381890421866 2316 40-41 -0.9319753159872164 2316 42-43 -0.9496615426222661 2316 44-45 -1.1231381797695619 2316 46-47 -1.076766712512601 2316 48-49 -1.2777516818972359 2316 50-51 -1.1376964144193025 2316 52-53 -0.94456848646373 2316 54-55 -0.9790082832927496 2316 56-57 -0.006590699330132566 2316 58-59 3.463267616382712 2316 60-61 3.2058497534274935 2316 62-63 0.9298359914066623 2316 64-65 0.3847888835140054 2316 66-67 5.261674800574806 2316 68-69 8.021484698188633 2316 70-71 4.984634112544697 2316 72-73 8.514957193431112 2316 74-75 9.424735115086982 2316 76-77 7.859478912630831 2316 78-79 5.450899932063233 2316 80-81 2.669935742763048 2316 82-83 1.364293896186119 2316 84-85 0.9196751607111224 2316 86-87 0.8689240410856165 2316 88-89 1.0750167985092922 2316 90-91 2.0765624829418456 2316 92-93 3.541677208555374 2316 94-95 7.320440420426177 2316 96-97 7.847495198320997 2316 98-99 8.171893992140074 2316 100-101 7.101120856068343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 43.0 5 119.0 6 293.0 7 695.0 8 1562.0 9 2701.0 10 4952.0 11 7700.0 12 11011.0 13 14384.0 14 17804.0 15 24184.0 16 31647.0 17 39053.0 18 36749.0 19 31138.0 20 30968.0 21 30094.0 22 28935.0 23 28245.0 24 29748.0 25 31644.0 26 40497.0 27 56841.0 28 77049.0 29 98775.0 30 117923.0 31 129479.0 32 136498.0 33 142225.0 34 146119.0 35 142780.0 36 120015.0 37 75228.0 38 24504.0 39 1104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50463988749806 21.439842646785717 14.932086530251834 21.12343093546439 2 15.529103603069677 22.821952058333913 39.75288198278314 21.896062355813275 3 18.057927603632955 28.680031880326162 31.7631396866269 21.49890082941398 4 11.718491366701912 19.080426617404154 39.10028965469727 30.100792361196664 5 11.450922655823232 40.42479851136486 34.338863133787285 13.785415699024616 6 27.265290965810664 39.576651737258764 18.706016779089722 14.452040517840848 7 25.56335532358009 36.17180534690544 21.60610347579798 16.658735853716497 8 26.065785110070017 34.68384939114675 20.419004068363453 18.831361430419776 9 27.53202637428338 16.00790317674308 21.45494120786184 35.005129241111696 10-11 23.770865093076605 28.250090936744808 27.822264935083137 20.156779035095454 12-13 25.34802645368882 26.787746339321632 28.23785899578976 19.62636821119979 14-15 22.617702646130994 27.178499550716488 28.01179055389446 22.192007249258054 16-17 19.798987463866148 30.4453886166463 28.532213794476537 21.223410125011018 18-19 19.56257665111725 28.078196473836954 32.76938351847348 19.589843356572313 20-21 21.184524813908094 26.985442530604665 32.60845702968771 19.221575625799527 22-23 20.73617600916432 27.270076001515708 32.26006566129259 19.733682328027378 24-25 19.78882823844286 27.880680481267028 32.86953160716027 19.460959673129842 26-27 19.92131850474798 27.878186145143886 32.964691416266916 19.23580393384122 28-29 19.36357139437309 27.904832025260728 33.12463726821658 19.606959312149602 30-31 19.822275656371964 27.88874317118573 32.82338625104811 19.46559492139419 32-33 19.56889193017447 27.919468729966145 32.56571687473653 19.94592246512285 34-35 19.962340604418703 27.87434606941006 32.76163062263534 19.401682703535897 36-37 20.135164572678697 27.503017900662446 32.32570200136309 20.036115525295763 38-39 19.462854429731056 28.217283021975277 32.24847362671665 20.071388921577014 40-41 19.661478028835454 27.96371710720349 32.56518989152977 19.809614972431287 42-43 19.663578048707127 27.850007110508397 32.1213347665315 20.36508007425298 44-45 19.70725525151957 27.96624893962158 31.917529205121355 20.408966603737497 46-47 20.005484114756417 28.160082419411548 31.114057906177834 20.7203755596542 48-49 19.603749160083364 28.353570664110144 31.000158565747988 21.0425216100585 50-51 19.483976880314103 28.545377668840928 30.645380764080272 21.325264686764697 52-53 19.852608606912195 28.898646590898803 30.125827831972995 21.122916970216007 54-55 19.814168084946726 28.46928237510741 30.147918653937623 21.56863088600824 56-57 20.037740030600183 28.668973911487523 29.718666023826977 21.574620034085317 58-59 18.608535545550783 28.181646464569194 31.417061558386994 21.79275643149303 60-61 20.176710172124178 28.56392748621859 29.77455996701363 21.484802374643603 62-63 19.547433586672902 28.324081972487054 30.06563824464113 22.06284619619891 64-65 19.073560480209288 28.344183125547097 30.393247224985103 22.189009169258515 66-67 19.916002384188516 28.235392401643338 29.458829496156504 22.38977571801164 68-69 20.903850623971785 27.55662655812755 29.09323628640536 22.446286531495304 70-71 20.029029353395046 27.893475413899743 29.81443015707807 22.26306507562714 72-73 19.869460983387974 27.675342977426514 29.873493823504464 22.58170221568105 74-75 20.04357259691313 28.077948842104078 29.114125727977758 22.76435283300504 76-77 18.198318883004013 28.408142508066845 30.312053872292523 23.08148473663662 78-79 18.867281691858775 28.405068680158752 29.223694889332997 23.503954738649476 80-81 19.12574304192797 28.244322053245035 28.981277182591697 23.648657722235296 82-83 19.501128755817113 28.430809005407458 28.46624957341409 23.60181266536134 84-85 19.22522477286249 28.12540687989752 28.423469212000263 24.225899135239725 86-87 19.18518070878704 27.940052076080473 28.686598510585565 24.188168704546918 88-89 19.479181901860102 27.729146797055108 28.51383041737205 24.27784088371274 90-91 19.227307520347566 27.680149391036068 28.743524710850394 24.34901837776597 92-93 19.273615905722377 27.5653721118763 28.94531355100446 24.215698431396863 94-95 18.465944899945853 27.41158160412111 29.346237946308367 24.776235549624666 96-97 18.21255055459186 27.253125569441718 29.560680892553297 24.973642983413125 98-99 18.333668128852914 27.185750158769768 29.127180706102923 25.35340100627439 100-101 18.060815992457805 27.257939123032077 29.59801923541455 25.08322564909557 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1086.0 1 799.0 2 528.5 3 568.5 4 627.0 5 722.0 6 966.5 7 1445.5 8 1982.5 9 2351.5 10 2600.5 11 2765.5 12 2963.5 13 3337.0 14 3658.5 15 3914.5 16 4204.5 17 4532.0 18 4864.5 19 5293.0 20 5908.0 21 6669.0 22 7561.0 23 8596.0 24 10021.0 25 12143.0 26 15175.5 27 18513.0 28 21322.0 29 24234.5 30 27702.5 31 31807.5 32 37441.0 33 43835.0 34 50097.0 35 55312.0 36 59499.5 37 62428.0 38 63415.5 39 63207.5 40 62298.5 41 61617.5 42 61129.5 43 61196.0 44 61695.0 45 61903.5 46 61688.5 47 60270.0 48 58624.0 49 56187.5 50 52118.0 51 47936.0 52 44180.0 53 40901.0 54 37730.5 55 34429.0 56 31307.0 57 28124.5 58 24701.5 59 21118.0 60 17363.5 61 13656.5 62 10248.5 63 7357.0 64 5332.5 65 4072.5 66 2949.0 67 2115.0 68 1523.5 69 1084.5 70 870.5 71 735.0 72 640.0 73 558.5 74 453.5 75 351.5 76 246.0 77 160.5 78 106.0 79 68.0 80 47.5 81 30.0 82 21.0 83 15.0 84 7.0 85 2.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023529700261979567 2 0.006188953418783708 3 0.004320590122547116 4 0.0 5 0.06387467019008844 6 1.1677270601478693E-4 7 0.0 8 5.8386353007393465E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1677270601478693E-4 16-17 0.0 18-19 0.0010217611776293857 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.8386353007393465E-5 28-29 0.0018099769432291973 30-31 0.007765384949983331 32-33 1.7515905902218038E-4 34-35 5.254771770665412E-4 36-37 0.026040313441297487 38-39 0.024434688733594164 40-41 0.026974495089415778 42-43 0.0277043245020082 44-45 0.028404960738096924 46-47 0.029835426386778063 48-49 0.029514301445237395 50-51 0.024639040969120042 52-53 0.020406030376084017 54-55 0.01561834942947775 56-57 0.15230080181978586 58-59 0.7775894493524661 60-61 0.31277569306060676 62-63 4.670908240591477E-4 64-65 0.14935229099291247 66-67 1.4563308030634152 68-69 2.7926192643436294 70-71 2.251465351494603 72-73 1.913408367581795 74-75 1.605361969114787 76-77 1.1495105180095624 78-79 0.43398576190395566 80-81 3.5031811804436076E-4 82-83 1.4596588251848365E-4 84-85 0.0 86-87 0.002481420002814222 88-89 2.9193176503696732E-5 90-91 0.22916643555401933 92-93 0.17837030843758706 94-95 1.3366971657512658 96-97 1.3123500565471828 98-99 1.0313657326991017 100-101 0.4160027651776785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1712729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.56516709256607 #Duplication Level Percentage of deduplicated Percentage of total 1 71.10448896476666 28.132609869225135 2 13.497933286203168 10.680959717458755 3 5.489462331774783 6.515744832150501 4 2.9374450195043433 4.648820120876614 5 1.7046015136092516 3.372142185609554 6 1.12691224861085 2.6751882848968584 7 0.7533364376008631 2.0864117421417636 8 0.5495560746967152 1.7394622337688206 9 0.39202737050846326 1.3959565577123993 >10 2.0807794400170785 15.033052321621277 >50 0.19566098335800747 5.4508270085455015 >100 0.15302566334186038 11.53579127418985 >500 0.011521119486281785 2.9479668751548918 >1k 0.0028064265415301787 1.6297855089667672 >5k 1.4770666008053572E-4 0.5138078813409287 >10k+ 2.9541332016107144E-4 1.6414735863403098 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15683 0.9156731742149516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12405 0.7242827090567159 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8792 0.5133328156410034 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2693 0.1572344486489106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2507 0.1463745869895354 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2292 0.13382152109294582 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.8386353007393465E-5 0.0 10-11 0.0 0.0 0.0 5.8386353007393465E-5 0.0 12-13 0.0 0.0 0.0 5.8386353007393465E-5 0.0 14-15 0.0 0.0 0.0 5.8386353007393465E-5 0.0 16-17 0.0 0.0 0.0 5.8386353007393465E-5 0.0 18-19 0.0 0.0 0.0 2.627385885332706E-4 0.0 20-21 0.0 0.0 0.0 6.130567065776313E-4 0.0 22-23 0.0 0.0 0.0 0.002277067767288345 0.0 24-25 0.0 0.0 0.0 0.005050419535139535 0.0 26-27 0.0 0.0 0.0 0.011035020718397365 0.0 28-29 0.0 0.0 0.0 0.029485108268733698 0.0 30-31 0.0 0.0 0.0 0.08249991679944696 0.0 32-33 0.0 0.0 0.0 0.1895221018619992 0.0 34-35 0.0 0.0 0.0 0.3494423227492499 0.0 36-37 0.0 0.0 0.0 0.6024595835067894 0.0 38-39 0.0 0.0 0.0 0.9831094119384911 0.0 40-41 0.0 0.0 0.0 1.4033743808857093 0.0 42-43 0.0 0.0 0.0 1.806181830283717 0.0 44-45 0.0 0.0 0.0 2.2684265870432507 0.0 46-47 0.0 0.0 0.0 2.7892328558692006 0.0 48-49 0.0 0.0 0.0 3.3007265013904714 0.0 50-51 0.0 0.0 0.0 3.810906453969075 0.0 52-53 2.9193176503696732E-5 0.0 0.0 4.287864571686472 0.0 54-55 5.8386353007393465E-5 5.8386353007393465E-5 0.0 4.777609300712489 0.0 56-57 5.8386353007393465E-5 5.8386353007393465E-5 0.0 5.1990419967198545 0.0 58-59 5.8386353007393465E-5 8.75795295110902E-5 0.0 5.4906234436387775 0.0 60-61 5.8386353007393465E-5 1.1677270601478693E-4 0.0 5.703295734468208 0.0 62-63 5.8386353007393465E-5 1.1677270601478693E-4 0.0 5.8748056464274265 0.0 64-65 5.8386353007393465E-5 1.1677270601478693E-4 0.0 6.052183386863888 0.0 66-67 5.8386353007393465E-5 1.4596588251848365E-4 0.0 6.197536212675794 2.9193176503696732E-5 68-69 5.8386353007393465E-5 1.7515905902218038E-4 0.0 6.363995705099873 5.8386353007393465E-5 70-71 5.8386353007393465E-5 1.7515905902218038E-4 0.0 6.668392956503919 5.8386353007393465E-5 72-73 5.8386353007393465E-5 1.7515905902218038E-4 0.0 7.042853831516837 5.8386353007393465E-5 74-75 5.8386353007393465E-5 1.7515905902218038E-4 0.0 7.478649570364022 5.8386353007393465E-5 76-77 5.8386353007393465E-5 1.7515905902218038E-4 0.0 8.084612335051254 5.8386353007393465E-5 78-79 5.8386353007393465E-5 1.7515905902218038E-4 0.0 8.9220477962363 5.8386353007393465E-5 80-81 5.8386353007393465E-5 1.7515905902218038E-4 0.0 9.85856489847489 5.8386353007393465E-5 82-83 5.8386353007393465E-5 1.7515905902218038E-4 0.0 10.702072540372704 5.8386353007393465E-5 84-85 5.8386353007393465E-5 1.7515905902218038E-4 0.0 11.37731071290321 5.8386353007393465E-5 86-87 5.8386353007393465E-5 1.7515905902218038E-4 0.0 11.98744810183047 5.8386353007393465E-5 88-89 5.8386353007393465E-5 1.7515905902218038E-4 0.0 12.599483047230471 5.8386353007393465E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7815 0.0 47.006138 1 GTATCAA 13455 0.0 38.062054 1 CATTCCG 525 0.0 31.4022 9 TTAGGCA 725 0.0 29.908934 4 ATCAACG 17785 0.0 28.758728 3 TCAACGC 17780 0.0 28.71295 4 GTACATG 13395 0.0 28.621601 1 TATCAAC 18010 0.0 28.454287 2 CAACGCA 18125 0.0 28.140408 5 TACATGG 13585 0.0 28.075064 2 AACGCAG 18460 0.0 27.634275 6 ACATGGG 13820 0.0 26.640158 3 CATGGGG 4110 0.0 24.42974 4 ACGCAGA 20830 0.0 24.42226 7 CGAAACG 50 0.0014158651 24.39339 66-67 CGCAGAG 20880 0.0 24.2961 8 CATGGGT 1305 0.0 23.840456 4 ATGGGAT 3495 0.0 23.063757 5 GCAGAGT 21375 0.0 23.050318 9 GAGTACT 11905 0.0 23.027401 12-13 >>END_MODULE