FastQCFastQC Report
Fri 27 May 2016
SRR939842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874820
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161910.8636029058789643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112910.6022444821369518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80050.4269743228683287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26190.13969341056741447No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.12545204339616603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.10721029218805005No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19140.10208980062085961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93100.044.0522161
GTATCAA162400.038.9237061
TATCACG959.403411E-734.6557582
TATCAAC204300.030.6875862
TCAACGC207200.030.3967134
ATCAACG208400.030.1765423
CAACGCA209700.030.0575625
AACGCAG214600.029.5278386
GTACATG146300.028.354711
TACATGG148250.027.632772
ACGCAGA240900.026.0112517
CGCAGAG240950.025.8287568
ACATGGG152700.025.8131353
GAGTACT128600.024.48820912-13
GCAGAGT242200.024.2394439
CATGGGA100950.023.6231274
CATGGGG43150.022.5644784
AGTACTT131700.022.50101912-13
GTACTTT139550.022.4902514-15
GTATAGG4650.022.2520851
Produced by FastQC (version 0.11.5)