FastQCFastQC Report
Fri 27 May 2016
SRR939842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874820
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171640.9155012214505925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138820.7404444159972691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95030.5068753266980297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28730.1532413778389392No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.14481390213460493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.12971911970215808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.044.8829961
GTATCAA153600.037.9085771
CATTCCG6250.029.5986449
ATCAACG200600.028.9426123
TCAACGC202100.028.7512684
TATCAAC202650.028.6824172
GTACATG152050.028.4325711
CAACGCA204450.028.3975945
AACGCAG208500.027.8687366
TACATGG154350.027.6977442
CGCATAC700.00843157927.1172811
ACATGGG154350.026.4288983
TTAGGCA8800.025.8729424
CCTTTAG8750.024.9478971
ACGCAGA236350.024.4845127
CGCAGAG236850.024.3727448
CATGGGG43750.024.1776474
CATGGGA103150.023.2685094
AGTACTT134750.023.09204112-13
GCAGAGT245350.023.0257059
Produced by FastQC (version 0.11.5)