FastQCFastQC Report
Fri 27 May 2016
SRR939841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298731
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86180.26125197841230463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66940.20292651931909572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.12350203760173231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.037.1307451
GTATCAA116950.036.4304961
ATCAACG143200.030.6166383
TATCAAC144550.030.5253332
TCAACGC145800.030.0385724
CAACGCA148250.029.2865455
TACATGG155150.028.92912
AACGCAG153400.028.488926
GTACATG159700.027.9856221
ACATGGG157900.027.3155733
ACGCAGA177400.024.367257
CGCAGAG180900.023.7897938
CATGGGG80300.023.0453994
CATGGGA88150.022.0697124
GCAGAGT188800.021.9144139
GAGTACT87550.021.81332212-13
AGTACAT116650.020.8247992
GTACTTT92500.020.5967514-15
GAGTACA125250.020.531021
TAGCGCG2902.4756446E-919.6344344
Produced by FastQC (version 0.11.5)