FastQCFastQC Report
Fri 27 May 2016
SRR939841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298731
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96650.2929914564115716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82700.2507024671002273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51190.15518088622564252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74900.041.005681
GTATCAA114650.033.290421
GTACATG155550.027.236491
TACATGG150200.026.6932582
ATCAACG142200.026.5020833
TATCAAC145650.026.1022032
TCAACGC144700.026.0442034
ACATGGG149800.025.818733
CAACGCA147550.025.5091825
AACGCAG152100.024.9143836
CATGGGG73600.021.3402374
CATGGGA83450.020.8560894
ACGCAGA182050.020.6343577
CGCAGAG184350.020.3257858
GAGTACT96900.020.16155812-13
AGTACAT114850.019.590252
GAGTACA121700.019.3443811
GTACTTT102350.018.92680414-15
GCAGAGT195900.018.766519
AGTACTT101800.018.47346912-13
Produced by FastQC (version 0.11.5)