##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939841_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3298731 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.430186335290752 31.0 31.0 34.0 30.0 34.0 2 31.63811295919552 31.0 31.0 34.0 30.0 34.0 3 31.69912369332328 31.0 31.0 34.0 30.0 34.0 4 35.50976996911843 37.0 35.0 37.0 33.0 37.0 5 35.24059585337513 37.0 35.0 37.0 33.0 37.0 6 35.26145629940726 37.0 35.0 37.0 32.0 37.0 7 35.175333181153604 37.0 35.0 37.0 32.0 37.0 8 35.12323102429389 37.0 35.0 37.0 32.0 37.0 9 36.72832856028576 39.0 35.0 39.0 32.0 39.0 10-11 36.58288914737213 38.5 35.0 39.0 32.0 39.0 12-13 36.51478750464952 38.0 35.0 39.0 32.0 39.0 14-15 37.57276555742193 39.0 36.0 41.0 32.0 41.0 16-17 37.49712828963622 39.0 36.0 41.0 32.0 41.0 18-19 37.46359584943423 39.0 36.0 41.0 32.0 41.0 20-21 37.38744656657363 39.0 36.0 41.0 32.0 41.0 22-23 37.244353662059744 39.0 36.0 40.0 31.0 41.0 24-25 37.07961546424974 39.0 36.0 40.0 31.0 41.0 26-27 36.79958959975821 39.0 36.0 40.0 30.5 41.0 28-29 36.57331986148613 38.0 35.0 40.0 30.0 41.0 30-31 36.32484476606307 38.0 35.0 40.0 30.0 41.0 32-33 36.29668681683957 38.0 35.0 40.0 30.0 41.0 34-35 36.39178626568823 38.0 35.0 40.0 30.0 41.0 36-37 36.36628661142724 38.0 35.0 40.0 30.0 41.0 38-39 36.24780589869256 38.0 35.0 40.0 30.0 41.0 40-41 36.073998001049496 38.0 35.0 40.0 29.5 41.0 42-43 35.86841000372567 38.0 35.0 40.0 29.0 41.0 44-45 35.621284972918374 38.0 34.0 40.0 28.0 41.0 46-47 35.31682910792059 38.0 34.0 40.0 27.0 41.0 48-49 35.02008893723071 38.0 34.0 40.0 26.0 41.0 50-51 34.761231667571565 38.0 33.5 40.0 26.0 41.0 52-53 34.480038384457536 38.0 33.0 40.0 24.5 41.0 54-55 34.172018876349725 37.5 33.0 40.0 24.0 41.0 56-57 32.896274052052135 36.5 31.5 39.5 16.0 41.0 58-59 29.560637257175564 34.5 24.5 39.0 8.0 40.0 60-61 29.529508013839262 34.0 25.0 38.0 9.5 40.0 62-63 31.39253913095672 35.0 29.5 38.5 12.5 40.0 64-65 30.964294602985206 35.0 29.5 38.0 6.5 40.0 66-67 25.526295414812544 29.0 18.0 36.0 2.0 39.0 68-69 17.80805209639707 13.5 7.0 30.0 2.0 35.5 70-71 16.642569369857682 15.0 7.5 26.5 2.0 33.5 72-73 18.824031119845785 19.5 7.5 30.0 2.0 35.0 74-75 19.550899573199512 20.0 8.5 31.5 2.0 35.0 76-77 20.501218347297794 23.5 9.0 31.5 2.0 34.5 78-79 23.602123968277496 29.0 12.0 34.0 2.0 36.0 80-81 26.261225301487144 31.5 23.0 35.0 2.0 36.0 82-83 27.21774039774689 33.0 26.0 35.0 2.0 36.0 84-85 27.201774258040437 33.0 26.0 35.0 2.0 36.0 86-87 26.906313821890905 33.0 26.0 35.0 2.0 35.5 88-89 26.48669260997638 33.0 25.0 35.0 2.0 35.0 90-91 24.88715660658599 31.5 12.5 34.5 2.0 35.0 92-93 22.810320999196357 31.0 2.0 34.0 2.0 35.0 94-95 18.38855911561143 24.0 2.0 33.0 2.0 35.0 96-97 17.083595328021595 15.5 2.0 32.5 2.0 34.5 98-99 15.998671610385934 6.0 2.0 32.0 2.0 34.0 100-101 15.365946480631491 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3628764410370273 1101 2 0.4515086728150557 1101 3 0.4636886802935791 1101 4 0.39715104603867246 1101 5 0.5092812347791451 1101 6 0.5292786630739741 1101 7 0.4016068671939408 1101 8 0.5410829202993455 1101 9 0.6548829902758584 1101 10-11 0.5281231603836076 1101 12-13 0.5370344863394365 1101 14-15 0.5223324972260599 1101 16-17 0.4520262204740533 1101 18-19 0.4640443170811537 1101 20-21 0.5418095013166067 1101 22-23 0.5308290205948438 1101 24-25 0.5704600841402225 1101 26-27 0.5547094437614817 1101 28-29 0.48755730542331577 1101 30-31 0.6068187546547179 1101 32-33 0.5437880871721745 1101 34-35 0.8155204269084138 1101 36-37 0.7248451888948964 1101 38-39 0.6430419324568319 1101 40-41 0.6558924862616138 1101 42-43 0.6345257725009148 1101 44-45 0.45808603567783024 1101 46-47 0.483671504354966 1101 48-49 0.36501355108099887 1101 50-51 0.3575087268943591 1101 52-53 0.373444288198975 1101 54-55 0.24115605563906684 1101 56-57 1.172507158636769 1101 58-59 -8.240044693999447 1101 60-61 -9.457346373954703 1101 62-63 -2.527375363369547 1101 64-65 0.07761542479181571 1101 66-67 -0.9199968650317949 1101 68-69 -3.6840653706358246 1101 70-71 -4.365768690893548 1101 72-73 -7.123693371207647 1101 74-75 -7.752119779348828 1101 76-77 -8.515806511083312 1101 78-79 -11.531835353025716 1101 80-81 -7.537515314141874 1101 82-83 -3.728520081399793 1101 84-85 -2.9086563529643534 1101 86-87 -2.695166382647084 1101 88-89 -2.73934300320202 1101 90-91 -8.687358688126817 1101 92-93 -14.740057725515145 1101 94-95 -12.756072154788143 1101 96-97 -12.745251876819259 1101 98-99 -12.172465126159466 1101 100-101 -11.907809377854825 1102 1 0.47888653013041704 1102 2 0.5358634118644012 1102 3 0.5065751413996082 1102 4 0.40971285926998746 1102 5 0.4591288958728157 1102 6 0.44595177804915664 1102 7 0.46955615642516335 1102 8 0.5397678465427731 1102 9 0.7281285961188999 1102 10-11 0.6413953230929224 1102 12-13 0.6578353510099646 1102 14-15 0.7526719539748115 1102 16-17 0.9078589918833586 1102 18-19 0.7986429165466689 1102 20-21 0.8550088966113876 1102 22-23 0.8712940878522915 1102 24-25 0.827869571806545 1102 26-27 0.8202128292187538 1102 28-29 0.858712382351321 1102 30-31 0.8472866617091128 1102 32-33 0.6982971510798066 1102 34-35 0.8624656058670794 1102 36-37 0.8818304032728861 1102 38-39 0.7542113112884152 1102 40-41 0.761343009565671 1102 42-43 0.9045075558705307 1102 44-45 0.8986282158109233 1102 46-47 1.007611087960683 1102 48-49 0.9980474605436953 1102 50-51 0.8900505128864964 1102 52-53 0.8734305656902066 1102 54-55 0.8720660256896977 1102 56-57 1.4263447916259793 1102 58-59 -8.651140085637941 1102 60-61 -9.840373422163015 1102 62-63 -2.7413223304473426 1102 64-65 0.032624369199265146 1102 66-67 -1.1449004929962818 1102 68-69 -3.76761562760886 1102 70-71 -4.3259070900180525 1102 72-73 -6.827317771657897 1102 74-75 -7.829375026016642 1102 76-77 -8.711713525076842 1102 78-79 -12.002069897383155 1102 80-81 -7.89965155807014 1102 82-83 -3.5859961041745834 1102 84-85 -2.5657370531324943 1102 86-87 -2.300720777718869 1102 88-89 -2.396108657439779 1102 90-91 -8.042340549638496 1102 92-93 -14.400269349471138 1102 94-95 -12.45426310883413 1102 96-97 -12.334796106561502 1102 98-99 -11.961627069678382 1102 100-101 -11.75401874445813 1103 1 0.23767087295321687 1103 2 0.22062000409929539 1103 3 0.21705476269100998 1103 4 0.1466931399661462 1103 5 0.23087535575144358 1103 6 0.20572361675319684 1103 7 0.181805536294668 1103 8 0.30977214052852986 1103 9 0.23397562710569275 1103 10-11 0.2554389122849656 1103 12-13 0.22118634366184864 1103 14-15 0.3923929536598081 1103 16-17 0.36690077244537633 1103 18-19 0.3826491488682109 1103 20-21 0.2264471240411794 1103 22-23 0.3181306368411896 1103 24-25 0.3472480959161075 1103 26-27 0.2548439452280675 1103 28-29 0.25230451493155925 1103 30-31 0.33897451633554 1103 32-33 0.36273596017145593 1103 34-35 0.21543274600005446 1103 36-37 0.10704834520498707 1103 38-39 0.14566712553043004 1103 40-41 0.0723829637738973 1103 42-43 0.1510712595258994 1103 44-45 0.0712264034750163 1103 46-47 0.02123694001071641 1103 48-49 -0.08423966692483731 1103 50-51 -0.023596265294898444 1103 52-53 0.05570854930538616 1103 54-55 -0.22445852454954007 1103 56-57 0.496257304687191 1103 58-59 -6.7198237171736785 1103 60-61 -8.742221104901116 1103 62-63 -2.620784994120303 1103 64-65 -0.3110746497901431 1103 66-67 -1.9319720712414856 1103 68-69 -4.372943352175049 1103 70-71 -5.019486594413145 1103 72-73 -7.793406160092012 1103 74-75 -8.482193689306126 1103 76-77 -9.039214932908006 1103 78-79 -12.33816058587906 1103 80-81 -8.68264123155804 1103 82-83 -4.927844298485091 1103 84-85 -4.022597803121819 1103 86-87 -3.7794444005216263 1103 88-89 -3.787326308540951 1103 90-91 -9.442904467590338 1103 92-93 -14.894182544091814 1103 94-95 -12.803480966981141 1103 96-97 -12.730747212323255 1103 98-99 -12.287650959707232 1103 100-101 -11.97247214826761 1104 1 0.16493899781739074 1104 2 0.1841949086655461 1104 3 0.23109422326957585 1104 4 0.22565072219645543 1104 5 0.1014307480523513 1104 6 0.16097297330168914 1104 7 0.14580191871218062 1104 8 0.2923274162691385 1104 9 0.20636742350713888 1104 10-11 0.2891114115310174 1104 12-13 0.18800492491930498 1104 14-15 0.30817536822057434 1104 16-17 0.42469739296890907 1104 18-19 0.36186823695233983 1104 20-21 0.29983649409241053 1104 22-23 0.30314228058374937 1104 24-25 0.24580481953231015 1104 26-27 0.2106504740797206 1104 28-29 0.2989550562523604 1104 30-31 0.16676162420402818 1104 32-33 0.2108164980761984 1104 34-35 0.3043845405899006 1104 36-37 0.2610488212765958 1104 38-39 0.11471790685311589 1104 40-41 0.06337793412649972 1104 42-43 0.1611520144013312 1104 44-45 0.18199788348545098 1104 46-47 0.11461065341490695 1104 48-49 0.16776349389572687 1104 50-51 0.17466412480997207 1104 52-53 0.09804069807706384 1104 54-55 0.01974773487929582 1104 56-57 0.734930548233983 1104 58-59 -6.492121844028507 1104 60-61 -8.486631959263576 1104 62-63 -2.501684850210456 1104 64-65 -0.28786339406701345 1104 66-67 -1.8067145502053314 1104 68-69 -4.304128271939781 1104 70-71 -4.846118719556781 1104 72-73 -7.935680915201267 1104 74-75 -8.733144735382204 1104 76-77 -9.287362782633686 1104 78-79 -12.363083908858886 1104 80-81 -8.558737319730998 1104 82-83 -4.67985645586538 1104 84-85 -3.7645795424754205 1104 86-87 -3.5768295877431058 1104 88-89 -3.5318867252229786 1104 90-91 -9.380283014034177 1104 92-93 -15.401360964917348 1104 94-95 -12.895233939181157 1104 96-97 -12.539971213845561 1104 98-99 -11.900610095840891 1104 100-101 -11.50057393385212 1105 1 0.13416464327278987 1105 2 0.13791466055916146 1105 3 0.045881616701493044 1105 4 0.05833841722639477 1105 5 0.08890378030542223 1105 6 0.08668545542204953 1105 7 0.20631186026839998 1105 8 0.12921816555996912 1105 9 0.2527126279066394 1105 10-11 0.15008339581958552 1105 12-13 0.17707369603954248 1105 14-15 0.22481338510405635 1105 16-17 0.2213035110697632 1105 18-19 0.20505294892449655 1105 20-21 0.2863283397672731 1105 22-23 0.15343437508298052 1105 24-25 0.27510179606123586 1105 26-27 0.24600322540526776 1105 28-29 0.20240032559927812 1105 30-31 0.24057805345440642 1105 32-33 0.1954201238458424 1105 34-35 0.2695778308802872 1105 36-37 0.1136095876222285 1105 38-39 0.27093379313641464 1105 40-41 0.2093838640762513 1105 42-43 0.24462489417150124 1105 44-45 0.18056928933423677 1105 46-47 0.1321653804247589 1105 48-49 0.24024703067436093 1105 50-51 0.11861510777819007 1105 52-53 0.17328114521450289 1105 54-55 0.24548462670379223 1105 56-57 0.841225790492075 1105 58-59 -6.938690849959968 1105 60-61 -8.919525799443196 1105 62-63 -2.7210170604189763 1105 64-65 -0.3352547857675887 1105 66-67 -1.801820080189593 1105 68-69 -4.172458355829367 1105 70-71 -4.795726009447833 1105 72-73 -6.354029077555744 1105 74-75 -6.854390721487597 1105 76-77 -8.714178055555417 1105 78-79 -12.159903023176721 1105 80-81 -8.165693410076582 1105 82-83 -4.192671017377968 1105 84-85 -3.300380367820214 1105 86-87 -3.032760652677098 1105 88-89 -3.1764798780121737 1105 90-91 -8.536434077313604 1105 92-93 -11.686685747715856 1105 94-95 -11.957692969398808 1105 96-97 -12.226514535827686 1105 98-99 -11.698111428955512 1105 100-101 -11.248334119127728 1106 1 0.08684292712979413 1106 2 0.14482639318358537 1106 3 0.1421181287402078 1106 4 0.12529663040261596 1106 5 0.08089042961336901 1106 6 0.04304035767258796 1106 7 0.08326155969823645 1106 8 0.16183614302967442 1106 9 0.08413306521911323 1106 10-11 0.1953807383437649 1106 12-13 0.22598206491822737 1106 14-15 0.2489246813477024 1106 16-17 0.1443686986169581 1106 18-19 0.28745491936587086 1106 20-21 0.18648184975271676 1106 22-23 0.18854567050615145 1106 24-25 0.24596849252615982 1106 26-27 0.30703021349814463 1106 28-29 0.16312587291903213 1106 30-31 0.1920057583101027 1106 32-33 0.2400919489609663 1106 34-35 0.298477163646524 1106 36-37 0.1684073376010531 1106 38-39 0.27136080232450155 1106 40-41 0.23027189732608377 1106 42-43 0.2533627656838533 1106 44-45 0.24612367789746514 1106 46-47 0.11890858663473125 1106 48-49 0.2197038228416872 1106 50-51 0.1828905199528137 1106 52-53 0.19891163330588313 1106 54-55 0.0395448140317356 1106 56-57 0.9491947537431216 1106 58-59 -6.77829634415701 1106 60-61 -9.270594858516048 1106 62-63 -2.692606363273601 1106 64-65 -0.22662333836415627 1106 66-67 -1.603675920100894 1106 68-69 -3.8099374007910463 1106 70-71 -4.457486962885323 1106 72-73 -6.179902771264533 1106 74-75 -6.593490432235367 1106 76-77 -8.408717160489825 1106 78-79 -11.764302849638145 1106 80-81 -7.814008542492733 1106 82-83 -3.8162921373399286 1106 84-85 -2.8370748517713125 1106 86-87 -2.6053245465706 1106 88-89 -2.7114559325876684 1106 90-91 -8.339460090140832 1106 92-93 -11.894175565452034 1106 94-95 -11.874992484528233 1106 96-97 -12.349011429208215 1106 98-99 -11.810292458607398 1106 100-101 -11.544889493258063 1107 1 0.06978031430284304 1107 2 0.10433155280307105 1107 3 0.058797720184063706 1107 4 0.011450016936393581 1107 5 0.029670536474334597 1107 6 -0.015995877712121853 1107 7 0.017033185241714932 1107 8 -0.022784249907765286 1107 9 0.005353612164199717 1107 10-11 0.08936853759625052 1107 12-13 0.01488929165454067 1107 14-15 0.06590384309568975 1107 16-17 0.029285256618635458 1107 18-19 -0.01883996169591029 1107 20-21 0.11498856579384409 1107 22-23 0.1879534765872748 1107 24-25 0.10254886890051296 1107 26-27 0.26154674207855066 1107 28-29 0.2341005161406926 1107 30-31 0.230201970956891 1107 32-33 0.15237174344792948 1107 34-35 0.06000458173007672 1107 36-37 0.12345699053901171 1107 38-39 0.14043418660627793 1107 40-41 -0.08253743466573837 1107 42-43 0.11827807963788928 1107 44-45 0.11727925286540852 1107 46-47 0.04091097665909871 1107 48-49 0.030432462452886 1107 50-51 0.1205464345366849 1107 52-53 0.03711284030450912 1107 54-55 0.052718642901631085 1107 56-57 0.5355549972495552 1107 58-59 -6.808574238974142 1107 60-61 -8.862355585475743 1107 62-63 -2.8404117088645826 1107 64-65 -0.48126994710844784 1107 66-67 -1.9002937450334265 1107 68-69 -4.143534678052035 1107 70-71 -4.926834264664041 1107 72-73 -6.511393521724923 1107 74-75 -7.13906598134165 1107 76-77 -9.229395547840857 1107 78-79 -12.554536303804525 1107 80-81 -8.559262591549981 1107 82-83 -4.343429038880366 1107 84-85 -3.3895611763279057 1107 86-87 -3.168619919803053 1107 88-89 -3.2034889879323494 1107 90-91 -8.739620363431767 1107 92-93 -12.169642628155668 1107 94-95 -12.221875872769168 1107 96-97 -12.642619612317498 1107 98-99 -11.940034868100662 1107 100-101 -11.52749870611189 1108 1 -0.031331203905775595 1108 2 -0.03438583442618537 1108 3 -0.03656917881220423 1108 4 -0.04845395538855257 1108 5 -0.1672858889444555 1108 6 -0.013731516164092739 1108 7 -0.0719905191941379 1108 8 -0.042770702081384115 1108 9 -0.02877753761170254 1108 10-11 0.04833779510720149 1108 12-13 -0.07689446288269863 1108 14-15 -0.003990160073989557 1108 16-17 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4.305337083396996 2311 62-63 1.7607115008352956 2311 64-65 1.4607072405392785 2311 66-67 -0.16897163794121894 2311 68-69 -3.31484611680812 2311 70-71 -0.7765956644519516 2311 72-73 -1.0187086784280872 2311 74-75 1.1673858067225922 2311 76-77 4.33790073165677 2311 78-79 5.179921623344157 2311 80-81 2.930091271409811 2311 82-83 1.4487305325974873 2311 84-85 1.0264951486538898 2311 86-87 1.0906377364848758 2311 88-89 1.1700275064651784 2311 90-91 2.599068644987913 2311 92-93 4.621152737825756 2311 94-95 8.558548848384802 2311 96-97 9.363959461134314 2311 98-99 9.840963835089616 2311 100-101 9.178999077173957 2312 1 -0.3490519768872318 2312 2 -0.2807505477510439 2312 3 -0.3147458210695575 2312 4 -0.2847868291822593 2312 5 -0.23009510838357983 2312 6 -0.21892722334782633 2312 7 -0.2988715730318887 2312 8 -0.36869828579816044 2312 9 -0.3727232522555042 2312 10-11 -0.39667627456378085 2312 12-13 -0.3426100093469344 2312 14-15 -0.5683979209224077 2312 16-17 -0.4497406571630762 2312 18-19 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1.575199271830833 2312 84-85 1.2377375756419973 2312 86-87 1.097400173227296 2312 88-89 1.3429016378619671 2312 90-91 2.903265737935726 2312 92-93 4.933350991894152 2312 94-95 9.00852516174865 2312 96-97 9.721698419302651 2312 98-99 10.272928377285949 2312 100-101 9.616790475932744 2313 1 -0.2692459869807209 2313 2 -0.24488846452112867 2313 3 -0.3282843335209513 2313 4 -0.2620797885601007 2313 5 -0.38441876189561697 2313 6 -0.29509935042651136 2313 7 -0.36528983231872303 2313 8 -0.285777792706412 2313 9 -0.3175466162002323 2313 10-11 -0.3042652130927834 2313 12-13 -0.3570791704290954 2313 14-15 -0.5210644221220448 2313 16-17 -0.46901953115992967 2313 18-19 -0.43116770403782567 2313 20-21 -0.5383733130942545 2313 22-23 -0.5092992598486674 2313 24-25 -0.4650816531878377 2313 26-27 -0.5222598568662491 2313 28-29 -0.6632362820323934 2313 30-31 -0.555950285126265 2313 32-33 -0.4492230671670825 2313 34-35 -0.46542852323882045 2313 36-37 -0.6459244568190528 2313 38-39 -0.8361854291115023 2313 40-41 -0.9115326311379022 2313 42-43 -0.6721211643970761 2313 44-45 -0.6440596320861616 2313 46-47 -0.5303418778963689 2313 48-49 -0.5741964453351258 2313 50-51 -0.45737446474672794 2313 52-53 -0.4543670006278475 2313 54-55 -0.4679538509955279 2313 56-57 0.5600210969424424 2313 58-59 4.191290907887815 2313 60-61 3.890801946613397 2313 62-63 1.3269941892376274 2313 64-65 1.1669466447491779 2313 66-67 1.9367808277675813 2313 68-69 0.40842216894379035 2313 70-71 3.0482310948130866 2313 72-73 8.475431425121286 2313 74-75 9.948914962039716 2313 76-77 8.861449203714383 2313 78-79 6.515511140876161 2313 80-81 3.518830762257405 2313 82-83 2.0898505500636446 2313 84-85 1.5571442729200662 2313 86-87 1.5020441088606198 2313 88-89 1.467256936943624 2313 90-91 2.9136283175667366 2313 92-93 4.832732558866933 2313 94-95 8.854972894850327 2313 96-97 9.634664622661635 2313 98-99 10.007715545317717 2313 100-101 9.165882268768021 2314 1 -0.4541325930437985 2314 2 -0.393476101932297 2314 3 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1.1125305174210176 2314 64-65 0.9411175237729985 2314 66-67 1.6860853472191586 2314 68-69 -0.14000601036673999 2314 70-71 2.602869477539702 2314 72-73 8.27754925562147 2314 74-75 9.667550192292595 2314 76-77 8.665490858491331 2314 78-79 6.365040443934873 2314 80-81 3.1736241529331473 2314 82-83 1.5741109756475993 2314 84-85 1.1317235467640465 2314 86-87 1.0428321253839314 2314 88-89 1.1952996176333635 2314 90-91 2.547966366022429 2314 92-93 4.677948985701004 2314 94-95 8.644743932856663 2314 96-97 9.452856366441612 2314 98-99 9.777719621792384 2314 100-101 8.901581961673598 2315 1 -0.784549954503877 2315 2 -0.5508645300158683 2315 3 -0.5501908741508252 2315 4 -0.41408446378298436 2315 5 -0.7228258912636818 2315 6 -0.7133173205543315 2315 7 -0.7104014671380341 2315 8 -0.896689235730598 2315 9 -1.0535337281229218 2315 10-11 -0.9592269321042579 2315 12-13 -0.7691766217736458 2315 14-15 -0.8557795474044951 2315 16-17 -1.0140125165914782 2315 18-19 -0.912717568168496 2315 20-21 -1.0995075055171313 2315 22-23 -1.0100189250916074 2315 24-25 -0.9765683428491059 2315 26-27 -1.007935431774733 2315 28-29 -1.0042139516002564 2315 30-31 -1.0210247054489159 2315 32-33 -0.9449216887046674 2315 34-35 -0.9709130660433019 2315 36-37 -1.0620064605058204 2315 38-39 -1.0416060864519991 2315 40-41 -0.9949156820676635 2315 42-43 -0.9240037566486237 2315 44-45 -0.8546632664823406 2315 46-47 -0.8233248469750478 2315 48-49 -0.9660814653730441 2315 50-51 -0.9424325842180892 2315 52-53 -0.8190631371526749 2315 54-55 -0.8979563308058829 2315 56-57 0.42762721288311667 2315 58-59 3.948977019389101 2315 60-61 3.5325521031189133 2315 62-63 0.8896777255512411 2315 64-65 0.8106644527355549 2315 66-67 1.7359302701570805 2315 68-69 6.1496081772641595 2315 70-71 4.978054299941956 2315 72-73 8.902101317275545 2315 74-75 9.92331402422053 2315 76-77 8.665943209683267 2315 78-79 6.113964536915745 2315 80-81 3.2072140472951283 2315 82-83 1.36233880564712 2315 84-85 0.9220497539946031 2315 86-87 0.9297803541502816 2315 88-89 1.0945948171739879 2315 90-91 2.330389338416218 2315 92-93 4.392868480220528 2315 94-95 8.534790151902616 2315 96-97 9.11917784282544 2315 98-99 9.457493038697665 2315 100-101 8.62140667929282 2316 1 -0.5702788149110845 2316 2 -0.5518786028736855 2316 3 -0.6322827895691603 2316 4 -0.42089197957588453 2316 5 -0.6221433629408821 2316 6 -0.4831169059855114 2316 7 -0.6144293376682768 2316 8 -0.6950317159120445 2316 9 -0.7321728713072488 2316 10-11 -0.7916744585380329 2316 12-13 -0.6283224824124645 2316 14-15 -0.7992334262559098 2316 16-17 -0.9273568536097798 2316 18-19 -0.9840035954028963 2316 20-21 -0.8321723822429661 2316 22-23 -0.9832493812453222 2316 24-25 -2.4082541639532806 2316 26-27 -1.5851782014604368 2316 28-29 -1.2240656047097005 2316 30-31 -0.8442242469651475 2316 32-33 -0.8692318208223355 2316 34-35 -0.7181754995229639 2316 36-37 -0.6562198237574606 2316 38-39 -0.9506226190503142 2316 40-41 -0.8146655781386514 2316 42-43 -0.9896753793337751 2316 44-45 -0.7582195655032464 2316 46-47 -0.7543828270820896 2316 48-49 -0.944749666008228 2316 50-51 -0.9191927092252854 2316 52-53 -0.8194439828224276 2316 54-55 -0.7618116109902857 2316 56-57 0.42584648219804677 2316 58-59 3.9332545181847287 2316 60-61 3.7492828083314897 2316 62-63 1.1688275286020193 2316 64-65 0.9302772037941338 2316 66-67 6.0734414806041634 2316 68-69 8.864115749654044 2316 70-71 5.542826953369179 2316 72-73 9.292066402866666 2316 74-75 10.106707145439135 2316 76-77 8.722228520274474 2316 78-79 6.241389013600081 2316 80-81 3.049039281907465 2316 82-83 1.738678576924272 2316 84-85 1.3424937901766576 2316 86-87 1.2368018066492859 2316 88-89 1.1968244064307605 2316 90-91 2.805387164703447 2316 92-93 4.537135750849412 2316 94-95 8.697970332510693 2316 96-97 9.403129889777357 2316 98-99 9.813442090088008 2316 100-101 8.91125856393852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 33.0 4 81.0 5 148.0 6 361.0 7 877.0 8 1821.0 9 3304.0 10 5238.0 11 8048.0 12 11326.0 13 15608.0 14 20465.0 15 28612.0 16 37868.0 17 49060.0 18 52339.0 19 53750.0 20 56326.0 21 56501.0 22 54236.0 23 52526.0 24 55042.0 25 60228.0 26 79570.0 27 114159.0 28 159327.0 29 208014.0 30 248065.0 31 267707.0 32 279167.0 33 289868.0 34 294974.0 35 287039.0 36 242782.0 37 153289.0 38 48854.0 39 2111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.12278805873121 19.177697567743923 13.599663797055678 24.09985057646919 2 16.17500917064061 20.809623564150527 38.71310901390603 24.30225825130284 3 19.4984125117057 24.454019530062236 29.73242947704895 26.31513848118312 4 13.302145582649812 16.3852402636044 35.85390866972784 34.45870548401794 5 13.580294475058682 37.91486201752241 33.95794062813014 14.546902879288764 6 33.078447207529685 35.704338157010824 16.85864975833109 14.358564877128396 7 29.19301391959514 32.41852700326277 20.230597766231924 18.157861310910164 8 26.83195669117208 35.46358943919882 19.152479123007147 18.551974746621955 9 26.908044335837022 15.994635512868433 19.509775122615334 37.58754502867921 10-11 25.59634295733723 26.49755314998404 26.943800509953675 20.962303382725054 12-13 25.9522980200568 23.67531635650194 27.67932577709428 22.693059846346976 14-15 22.662016788610266 25.027385584943513 25.671740698563944 26.638856927882276 16-17 22.640933740883995 28.496518812840456 26.167865764137787 22.69468168213777 18-19 22.513479212432934 26.977599141234982 28.320904916663142 22.188016729668938 20-21 23.223430464624123 26.164925239432986 28.43808725234037 22.173557043602525 22-23 22.907460474952337 26.13233695017872 28.301686315131487 22.658516259737457 24-25 22.65771292051398 26.344130515643744 28.390781182218255 22.607375381624024 26-27 22.57319115718952 26.714185026032784 28.294416782241623 22.41820703453607 28-29 22.31259069035854 26.749352880287137 28.54934381598806 22.388712613366263 30-31 22.66334024266645 26.349571024094136 28.474622980520774 22.512465752718636 32-33 22.461894310520446 26.52187818305282 28.421645365475097 22.59458214095164 34-35 22.68298664593929 26.42525215259237 28.31781761101429 22.57394359045405 36-37 22.780555089778417 26.366521900099848 28.268519897039162 22.58440311308257 38-39 22.377979133293977 26.694074344273055 28.281727517532694 22.64621900490027 40-41 22.646170164089366 26.402861675139413 28.367701119050942 22.58326704172028 42-43 22.588727513761764 26.40939432476244 28.23553794438046 22.766340217095333 44-45 22.39899729584018 26.44824769351966 28.16551653455157 22.987238476088592 46-47 22.457660011377182 26.721981862001293 27.892418889038485 22.92793923758304 48-49 22.613832618394703 26.743313909987325 27.755750703757055 22.88710276786092 50-51 22.311427666119364 26.791696798519105 27.7224109726941 23.17446456266743 52-53 22.69774473386671 26.749255556906498 27.57156159161797 22.981438117608818 54-55 22.280503670563967 26.499037221668225 27.803540465341342 23.416918642426463 56-57 22.23031875229233 26.7405009485085 27.8303900422396 23.198790256959576 58-59 20.947651145696728 26.318441976457624 29.452054899123187 23.28185197872246 60-61 22.440866046663103 26.402545575461616 28.17172538668119 22.98486299119409 62-63 22.23085856609274 26.574678489026503 27.76028951827087 23.43417342660989 64-65 21.466990965315688 26.57724471423458 28.32366397568003 23.632100344769697 66-67 21.46286204801117 26.440809388187226 28.647197565114567 23.449130998687032 68-69 21.825434342600502 25.828200553397913 29.017364358713372 23.329000745288216 70-71 20.97400397154875 26.16744615967163 29.27002301837395 23.58852685040567 72-73 20.99986274316962 26.12423611324476 29.098911224474517 23.77698991911111 74-75 21.360230918419795 26.268417263340872 28.433285145794883 23.93806667244445 76-77 19.945556042635502 26.557109442777133 29.023641430134855 24.47369308445251 78-79 21.09865901855567 26.66806892858007 27.895829392008352 24.337442660855903 80-81 21.671218171987938 26.894674928241223 27.189258396917232 24.244848502853603 82-83 21.833946296875645 26.676327966416146 26.97479149538853 24.514934241319686 84-85 21.85417665156692 26.384676410413583 26.84564155125107 24.915505386768427 86-87 21.62158598060818 26.320146107835622 27.186786026263093 24.8714818852931 88-89 21.6438902244197 26.305093172220822 27.157572762851157 24.89344384050832 90-91 21.556588138321935 26.383315058562818 27.274160750532523 24.785936052582727 92-93 21.196708475491892 26.04957692914434 27.44554601564563 25.30816857971814 94-95 19.9681142422028 25.9772055978925 28.423796569786365 25.63088359011833 96-97 19.56289887867734 25.942563465671437 28.7351343519846 25.759403303666627 98-99 19.95100276857013 25.810035615145786 28.12284638278031 26.116115233503773 100-101 19.892352033502654 25.929220777244495 28.142014967845608 26.036412221407247 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 307.0 1 249.5 2 202.5 3 246.5 4 292.0 5 342.5 6 491.5 7 746.5 8 1069.5 9 1365.5 10 1618.5 11 1852.5 12 2113.0 13 2517.5 14 2928.0 15 3285.5 16 3641.5 17 3939.5 18 4375.5 19 4863.5 20 5428.5 21 6247.5 22 7256.0 23 8689.5 24 10414.0 25 12595.0 26 15652.0 27 19099.0 28 22921.0 29 27217.5 30 32139.5 31 37800.5 32 44234.0 33 50847.5 34 57366.0 35 64441.0 36 71650.5 37 78371.0 38 84477.0 39 89682.0 40 94955.5 41 101361.0 42 108221.0 43 115407.0 44 121883.5 45 127045.5 46 131243.0 47 134559.0 48 137737.0 49 139234.5 50 137205.0 51 133112.0 52 128488.0 53 122872.0 54 115767.0 55 106061.5 56 95410.5 57 86129.0 58 76756.5 59 66577.5 60 55748.0 61 44444.5 62 34053.5 63 25938.5 64 20047.5 65 15427.0 66 11555.5 67 8552.5 68 5930.5 69 3872.5 70 2798.0 71 2188.0 72 1702.5 73 1291.0 74 937.5 75 621.5 76 352.5 77 190.0 78 101.0 79 52.5 80 30.0 81 19.0 82 12.5 83 7.0 84 3.0 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0219175191914709 2 0.0048806647162196615 3 0.003759021272119491 4 0.0 5 0.06526752257155857 6 9.094406303514897E-5 7 0.0 8 1.2125875071353196E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9704546990948946E-4 16-17 0.0 18-19 0.0012277448509745111 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.8188812607029795E-4 28-29 0.0022887589197179157 30-31 0.0089125181774446 32-33 3.334615644622129E-4 34-35 0.0010306993810650217 36-37 0.023584827013781968 38-39 0.022736015758787243 40-41 0.024327536861902352 42-43 0.025009617334665966 44-45 0.02573716983894716 46-47 0.027374162973579842 48-49 0.027146802815991966 50-51 0.02200846325450605 52-53 0.019037623862024518 54-55 0.014066015082769707 56-57 0.15578717997921018 58-59 0.7506674536359589 60-61 0.30925528635102406 62-63 3.031468767838299E-4 64-65 0.14090266832912413 66-67 1.3997958608931738 68-69 2.684774842204472 70-71 2.168303508227861 72-73 1.8272632718460524 74-75 1.5356662910676864 76-77 1.1113667649771988 78-79 0.4111429516380693 80-81 1.6673078223110644E-4 82-83 9.094406303514897E-5 84-85 0.0 86-87 0.002167500169004384 88-89 3.031468767838299E-5 90-91 0.22153973755362288 92-93 0.16995929646885424 94-95 1.2668811127673036 96-97 1.2406740652693415 98-99 0.9829234332838901 100-101 0.3947275482602249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3298731.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.42913350390036 #Duplication Level Percentage of deduplicated Percentage of total 1 69.87258912570644 30.345060014034818 2 14.763480032802544 12.82330290653498 3 5.814275433005673 7.575268320253545 4 2.866356021288805 4.979334332730407 5 1.6538579335660972 3.591280849666341 6 1.080315070501949 2.8150288453862804 7 0.7078823522510028 2.1519902026674678 8 0.5202270142989104 1.8074406765058288 9 0.3769263640559377 1.4732626847152563 >10 2.0736233533306367 16.47570061927445 >50 0.1667162914096078 5.007849522485535 >100 0.0969613040851067 7.820762822151554 >500 0.0050397800645143745 1.4624383074529048 >1k 0.001539932796919065 0.970459809458178 >5k 2.0999083594350885E-4 0.7008200866823968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9665 0.2929914564115716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8270 0.2507024671002273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5119 0.15518088622564252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5157343839191495E-5 0.0 12-13 0.0 0.0 0.0 4.547203151757448E-5 0.0 14-15 0.0 0.0 0.0 9.094406303514896E-5 0.0 16-17 0.0 0.0 0.0 1.2125875071353196E-4 0.0 18-19 0.0 0.0 0.0 1.2125875071353196E-4 0.0 20-21 0.0 0.0 0.0 1.8188812607029792E-4 0.0 22-23 0.0 0.0 0.0 4.092482836581704E-4 0.0 24-25 0.0 0.0 0.0 8.033392234771492E-4 0.0 26-27 0.0 0.0 0.0 0.002046241418290852 0.0 28-29 0.0 0.0 0.0 0.007123951604420003 0.0 30-31 0.0 0.0 0.0 0.027237746879027115 0.0 32-33 0.0 0.0 0.0 0.07563514575756555 0.0 34-35 0.0 0.0 0.0 0.15368030918556258 0.0 36-37 0.0 0.0 0.0 0.28394252213957427 0.0 38-39 0.0 0.0 0.0 0.48012402345022975 0.0 40-41 3.031468767838299E-5 0.0 0.0 0.6981624145769995 0.0 42-43 3.031468767838299E-5 0.0 0.0 0.9127600886522726 0.0 44-45 3.031468767838299E-5 0.0 0.0 1.1680855456234533 0.0 46-47 3.031468767838299E-5 0.0 0.0 1.46717025425838 0.0 48-49 3.031468767838299E-5 0.0 0.0 1.7578426370625553 0.0 50-51 3.031468767838299E-5 1.5157343839191495E-5 0.0 2.0476358939240575 0.0 52-53 3.031468767838299E-5 3.031468767838299E-5 0.0 2.3235601811726996 0.0 54-55 3.031468767838299E-5 3.031468767838299E-5 0.0 2.621750606521114 3.031468767838299E-5 56-57 3.031468767838299E-5 3.031468767838299E-5 0.0 2.8901871659131952 3.031468767838299E-5 58-59 3.031468767838299E-5 3.031468767838299E-5 0.0 3.0787596806165762 4.547203151757448E-5 60-61 3.031468767838299E-5 3.031468767838299E-5 0.0 3.2212690273926547 6.062937535676598E-5 62-63 4.547203151757448E-5 3.031468767838299E-5 0.0 3.3394053652753133 6.062937535676598E-5 64-65 6.062937535676598E-5 3.031468767838299E-5 0.0 3.4638168435073973 6.062937535676598E-5 66-67 6.062937535676598E-5 3.031468767838299E-5 1.5157343839191495E-5 3.566674578800151 6.062937535676598E-5 68-69 6.062937535676598E-5 3.031468767838299E-5 3.031468767838299E-5 3.6852959516856636 6.062937535676598E-5 70-71 6.062937535676598E-5 3.031468767838299E-5 3.031468767838299E-5 3.9017579790531567 6.062937535676598E-5 72-73 6.062937535676598E-5 3.031468767838299E-5 3.031468767838299E-5 4.164525691849381 6.062937535676598E-5 74-75 6.062937535676598E-5 3.031468767838299E-5 3.031468767838299E-5 4.467657411289371 6.062937535676598E-5 76-77 6.062937535676598E-5 4.547203151757448E-5 3.031468767838299E-5 4.893305940981547 6.062937535676598E-5 78-79 6.062937535676598E-5 6.062937535676598E-5 3.031468767838299E-5 5.4804559692803085 6.062937535676598E-5 80-81 6.062937535676598E-5 6.062937535676598E-5 3.031468767838299E-5 6.141513206138967 6.062937535676598E-5 82-83 6.062937535676598E-5 7.578671919595748E-5 3.031468767838299E-5 6.760251138998603 6.062937535676598E-5 84-85 6.062937535676598E-5 9.094406303514897E-5 3.031468767838299E-5 7.25709371270346 6.062937535676598E-5 86-87 6.062937535676598E-5 9.094406303514897E-5 3.031468767838299E-5 7.699900355621601 6.062937535676598E-5 88-89 6.062937535676598E-5 9.094406303514897E-5 3.031468767838299E-5 8.170429780421623 6.062937535676598E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7490 0.0 41.00568 1 GTATCAA 11465 0.0 33.29042 1 GTACATG 15555 0.0 27.23649 1 TACATGG 15020 0.0 26.693258 2 ATCAACG 14220 0.0 26.502083 3 TATCAAC 14565 0.0 26.102203 2 TCAACGC 14470 0.0 26.044203 4 ACATGGG 14980 0.0 25.81873 3 CAACGCA 14755 0.0 25.509182 5 AACGCAG 15210 0.0 24.914383 6 CATGGGG 7360 0.0 21.340237 4 CATGGGA 8345 0.0 20.856089 4 ACGCAGA 18205 0.0 20.634357 7 CGCAGAG 18435 0.0 20.325785 8 GAGTACT 9690 0.0 20.161558 12-13 AGTACAT 11485 0.0 19.59025 2 GAGTACA 12170 0.0 19.344381 1 GTACTTT 10235 0.0 18.926804 14-15 GCAGAGT 19590 0.0 18.76651 9 AGTACTT 10180 0.0 18.473469 12-13 >>END_MODULE