FastQCFastQC Report
Fri 27 May 2016
SRR939840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470308
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90780.261590613859058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70050.20185528200955075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.12373541483925922No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35110.10117257603647861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78200.040.2395061
GTATCAA126350.036.5941431
ATCAACG154350.029.5930523
TATCAAC157100.029.193122
TCAACGC156350.029.124664
TACATGG158350.028.6062092
CAACGCA159750.028.5354775
GTACATG165950.028.4570391
AACGCAG163750.028.011616
ACATGGG160900.027.2481483
ACGCAGA192200.023.693917
CGCAGAG194700.023.1960378
CATGGGG79200.023.1644974
CATGGGA89700.021.4493584
GCAGAGT200400.021.221969
GAGTACT92450.020.81147812-13
GTACTTT98550.019.36112214-15
AGTACTT96600.019.08954212-13
AGAGTAC171400.018.73202310-11
CAGAGTA193700.018.45766610-11
Produced by FastQC (version 0.11.5)