##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3470308 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.063076821999662 31.0 30.0 33.0 26.0 34.0 2 30.12280523803651 31.0 30.0 33.0 26.0 34.0 3 30.074351613747254 31.0 30.0 34.0 25.0 34.0 4 33.71995915059989 35.0 33.0 37.0 28.0 37.0 5 33.59136191946075 35.0 33.0 37.0 28.0 37.0 6 33.56288721346924 35.0 33.0 37.0 28.0 37.0 7 33.551791656533084 35.0 33.0 37.0 28.0 37.0 8 33.391357769973155 35.0 33.0 37.0 27.0 37.0 9 34.687461746911225 37.0 34.0 39.0 27.0 39.0 10-11 34.492408310732074 37.0 33.5 39.0 27.0 39.0 12-13 34.38744961542318 37.0 33.0 39.0 27.0 39.0 14-15 35.00822852035036 38.0 33.0 40.0 26.0 41.0 16-17 34.79066973306116 38.0 33.0 40.0 25.0 41.0 18-19 34.80422976865454 38.0 33.0 40.0 25.0 41.0 20-21 34.926256401449095 38.0 33.5 40.0 25.0 41.0 22-23 34.83963368669294 38.0 33.0 40.0 25.0 41.0 24-25 34.694568897054666 38.0 33.0 40.0 25.0 41.0 26-27 34.58505095224977 38.0 33.0 40.0 24.0 41.0 28-29 34.462764400162754 38.0 33.0 40.0 24.0 41.0 30-31 34.280026729615926 38.0 33.0 40.0 23.5 41.0 32-33 34.03094336295222 38.0 33.0 40.0 23.0 41.0 34-35 33.75305909446654 38.0 32.0 40.0 21.0 41.0 36-37 33.55097602287751 38.0 32.0 40.0 19.5 41.0 38-39 33.290325239143044 37.0 31.0 40.0 18.0 41.0 40-41 32.99675806873626 37.0 31.0 40.0 17.0 41.0 42-43 32.691068631372204 37.0 31.0 40.0 15.5 41.0 44-45 32.295126830241 37.0 30.0 40.0 13.5 41.0 46-47 31.919068854983475 36.5 30.0 39.0 9.0 41.0 48-49 31.58629104390734 36.0 30.0 39.0 8.5 40.0 50-51 30.838137421808092 35.5 28.5 38.5 8.0 40.0 52-53 30.94498427805255 35.5 29.0 39.0 7.0 40.0 54-55 31.029815365091515 36.0 29.0 39.0 4.0 40.0 56-57 30.816225533871922 35.5 28.5 39.0 2.0 40.0 58-59 30.288336222606176 35.0 28.0 39.0 2.0 40.0 60-61 29.798879234926698 34.5 27.0 38.0 2.0 40.0 62-63 29.36803145426861 34.0 26.0 38.0 2.0 40.0 64-65 28.86425484423861 34.0 26.0 38.0 2.0 40.0 66-67 28.32326381404763 34.0 26.0 37.0 2.0 39.0 68-69 27.80304932588116 33.0 25.0 36.5 2.0 39.0 70-71 27.27410549726422 32.5 23.5 36.0 2.0 39.0 72-73 26.80295149594791 32.0 22.5 36.0 2.0 38.5 74-75 26.208279063414544 32.0 21.5 35.0 2.0 38.0 76-77 25.56545139509231 31.0 19.5 35.0 2.0 37.0 78-79 24.91264377686361 31.0 18.0 34.5 2.0 36.0 80-81 24.238409097982082 30.5 12.5 34.0 2.0 36.0 82-83 23.502835915428832 30.0 7.0 34.0 2.0 35.0 84-85 22.774207793659812 29.5 2.0 34.0 2.0 35.0 86-87 21.9939021262666 29.0 2.0 34.0 2.0 35.0 88-89 21.161109330929705 28.5 2.0 33.5 2.0 35.0 90-91 20.215382467492798 26.5 2.0 33.0 2.0 34.0 92-93 19.237170303039385 25.5 2.0 32.5 2.0 34.0 94-95 17.868436173388645 23.5 2.0 32.0 2.0 34.0 96-97 15.284837974035733 5.0 2.0 31.0 2.0 34.0 98-99 13.884607360499414 2.0 2.0 30.5 2.0 34.0 100-101 12.605921434062914 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6467512063230885 1101 2 0.6862802280030316 1101 3 0.7495738104256624 1101 4 0.8591925260649091 1101 5 0.7940446138929502 1101 6 0.7685555785600116 1101 7 0.7988586656166632 1101 8 0.5361767427026365 1101 9 0.7664690935821312 1101 10-11 0.7876488099636205 1101 12-13 0.5202710444136613 1101 14-15 0.8333611707787654 1101 16-17 0.8902506073055108 1101 18-19 0.7994011054072203 1101 20-21 0.9433911720817179 1101 22-23 0.8834218447742117 1101 24-25 0.8380432565596578 1101 26-27 0.9757229440354749 1101 28-29 1.0133786934437623 1101 30-31 0.9727228828200083 1101 32-33 0.8514491142854581 1101 34-35 0.6717489399514207 1101 36-37 0.7098067753885999 1101 38-39 0.5881259506666652 1101 40-41 0.7891532555748952 1101 42-43 0.7045887909617363 1101 44-45 0.6958685647395342 1101 46-47 0.7261074672876333 1101 48-49 0.5975144411011364 1101 50-51 0.5413166050992437 1101 52-53 0.68126153360625 1101 54-55 0.6359463147819646 1101 56-57 0.2950033783192332 1101 58-59 0.32950554675065646 1101 60-61 0.5804875751061083 1101 62-63 0.4640673396610495 1101 64-65 0.5795989909514141 1101 66-67 0.24385644709224863 1101 68-69 0.16994811031007728 1101 70-71 0.12588289832685007 1101 72-73 -0.06145679356456313 1101 74-75 0.01111172579000197 1101 76-77 -0.2772420769120103 1101 78-79 -0.22094410611887483 1101 80-81 -0.41703512266652965 1101 82-83 -0.3652612965216022 1101 84-85 -0.32975302900671366 1101 86-87 -0.5277029252503205 1101 88-89 -0.586171770922661 1101 90-91 -0.8301205265355023 1101 92-93 -0.9594914215052235 1101 94-95 -0.7536815116145767 1101 96-97 0.9460597676219606 1101 98-99 0.8655759231002129 1101 100-101 0.2730416615901188 1102 1 0.8031991624902872 1102 2 0.8475799923240821 1102 3 0.8050428064536597 1102 4 0.9895431928302543 1102 5 1.0127905776732717 1102 6 0.9509150673224553 1102 7 0.811627269233405 1102 8 0.7454678845700471 1102 9 0.9813513520656301 1102 10-11 0.8538053528843292 1102 12-13 0.7240079398514467 1102 14-15 0.9168424837165716 1102 16-17 0.9768887428876596 1102 18-19 1.0663383529847579 1102 20-21 0.915505474557655 1102 22-23 0.7917119043905387 1102 24-25 0.957797304751935 1102 26-27 1.2142196726114065 1102 28-29 1.2598706289861425 1102 30-31 1.188054386158548 1102 32-33 1.2006016169956197 1102 34-35 1.0822516189128706 1102 36-37 0.9350794877862754 1102 38-39 1.0388297857465503 1102 40-41 1.0803165739025573 1102 42-43 0.9238869005234207 1102 44-45 0.8532246703335673 1102 46-47 0.8573045157135653 1102 48-49 0.6769588087515821 1102 50-51 0.9493535166456262 1102 52-53 1.047631869187537 1102 54-55 0.9163451712891835 1102 56-57 0.8382016316247594 1102 58-59 0.5268200003716821 1102 60-61 0.7635195252768447 1102 62-63 0.42517364661255996 1102 64-65 0.3251413718022107 1102 66-67 0.28488276070488894 1102 68-69 0.04644001501393191 1102 70-71 0.38329707106326794 1102 72-73 0.150371679253265 1102 74-75 0.23620648171571546 1102 76-77 0.15257577257563426 1102 78-79 -0.16861737513390906 1102 80-81 -0.47486571536135 1102 82-83 -0.4859648540546928 1102 84-85 -0.7747790821244536 1102 86-87 -0.8558433218411032 1102 88-89 -0.6665895477764892 1102 90-91 -0.7723413616284454 1102 92-93 -0.9666659607049866 1102 94-95 -0.7229530002667239 1102 96-97 0.6824502697893653 1102 98-99 0.5524818278447032 1102 100-101 0.13934950379261224 1103 1 0.7923194576394863 1103 2 0.7243861563929741 1103 3 0.8771780159378757 1103 4 0.8286804360874314 1103 5 0.8956434344799717 1103 6 0.9673099106154837 1103 7 0.8632993891755874 1103 8 0.8717158816118271 1103 9 0.9131716348889327 1103 10-11 0.971577194933559 1103 12-13 0.8642545838459341 1103 14-15 1.0361722210629267 1103 16-17 1.0052004872261548 1103 18-19 0.9482870772544558 1103 20-21 0.8544653229491246 1103 22-23 0.870063637859829 1103 24-25 0.9446096457758841 1103 26-27 1.1549261565933406 1103 28-29 1.1964440857278547 1103 30-31 1.0559866049320092 1103 32-33 1.0721741773084688 1103 34-35 1.1811340242555843 1103 36-37 1.1241156726971298 1103 38-39 1.071201722481085 1103 40-41 1.1630811184606742 1103 42-43 1.0996048139732153 1103 44-45 1.0698169380744602 1103 46-47 1.0749696967361615 1103 48-49 0.9668824582254949 1103 50-51 1.1776560865950962 1103 52-53 1.2815371194592622 1103 54-55 1.1338989990403796 1103 56-57 0.937889422263801 1103 58-59 0.7976717758610725 1103 60-61 1.1625949429566624 1103 62-63 0.8674649887906583 1103 64-65 0.7886069937557529 1103 66-67 0.5247489126719884 1103 68-69 0.3209065698137472 1103 70-71 0.5396234075826598 1103 72-73 0.2787798597268072 1103 74-75 0.24689394459796077 1103 76-77 0.287173163967676 1103 78-79 0.20311906357836662 1103 80-81 -0.0028819183128412362 1103 82-83 -0.08191565691878466 1103 84-85 -0.08754731044488295 1103 86-87 -0.3213889388642883 1103 88-89 -0.07891635492102722 1103 90-91 -0.07883178664777546 1103 92-93 -0.12442868439200083 1103 94-95 -0.10140074967003798 1103 96-97 1.3298686947638743 1103 98-99 1.484609557750689 1103 100-101 1.0090560213906326 1104 1 0.40776249442362555 1104 2 0.5163667250375745 1104 3 0.5499381430975703 1104 4 0.7843015152518618 1104 5 0.7293600338987218 1104 6 0.8657596815963657 1104 7 0.5694789081884082 1104 8 0.7187633219586971 1104 9 0.884296179907416 1104 10-11 0.745068114113792 1104 12-13 0.6429683851871317 1104 14-15 0.8608268113209121 1104 16-17 0.9601361549556202 1104 18-19 0.9142563407251956 1104 20-21 0.6156717411276986 1104 22-23 0.6520669438237263 1104 24-25 0.721363617164954 1104 26-27 0.9304033581395785 1104 28-29 0.871533788802445 1104 30-31 0.6670561759561551 1104 32-33 0.9427625025062198 1104 34-35 0.9333348650896482 1104 36-37 0.7637121146711152 1104 38-39 0.5401195483513064 1104 40-41 0.5686260146731641 1104 42-43 0.4231372340516515 1104 44-45 0.5946739774732706 1104 46-47 0.4662573297036374 1104 48-49 0.3841998542733869 1104 50-51 0.6368223770496506 1104 52-53 0.4764020593123526 1104 54-55 0.35879859743395315 1104 56-57 0.4962734266654856 1104 58-59 0.4564656163283267 1104 60-61 0.5649367457650847 1104 62-63 0.35961038536524725 1104 64-65 0.34332998407769466 1104 66-67 0.3652373300417011 1104 68-69 0.06636909908775479 1104 70-71 0.3462621405002757 1104 72-73 0.4041654771379015 1104 74-75 0.570385216477046 1104 76-77 0.31194908263061905 1104 78-79 0.20165712683843395 1104 80-81 0.1603024350255673 1104 82-83 0.37349262727184396 1104 84-85 0.2308780302622111 1104 86-87 0.23730250136971875 1104 88-89 0.0871548385178329 1104 90-91 -0.19803988367287673 1104 92-93 0.09794852938112797 1104 94-95 0.4591388325464152 1104 96-97 1.6763247767028702 1104 98-99 2.1268430114718964 1104 100-101 1.9775247440564705 1105 1 0.5689884832981811 1105 2 0.7667793382852253 1105 3 0.6664034362497304 1105 4 0.6545658728811858 1105 5 0.731060673434996 1105 6 0.927688738351641 1105 7 0.7031496319810486 1105 8 0.6495378258247726 1105 9 0.8651768169115499 1105 10-11 0.93560276050966 1105 12-13 0.6625773521541163 1105 14-15 0.8788643120765656 1105 16-17 0.9376368991992834 1105 18-19 0.8885890592733432 1105 20-21 0.8037974333313258 1105 22-23 0.7122520181169278 1105 24-25 0.8181957055297744 1105 26-27 1.007658416430111 1105 28-29 0.9014409779110579 1105 30-31 1.0816278394918655 1105 32-33 0.96270424048911 1105 34-35 1.068952313582848 1105 36-37 1.0369102751061021 1105 38-39 0.8980893428944299 1105 40-41 0.8690153025831151 1105 42-43 0.756236743706296 1105 44-45 0.8120712575906168 1105 46-47 0.7765581752328643 1105 48-49 0.8202345354576543 1105 50-51 0.7448189757705812 1105 52-53 0.6608182243987137 1105 54-55 0.8337711944394108 1105 56-57 0.8084631112772627 1105 58-59 0.7027649288867117 1105 60-61 0.932005703219442 1105 62-63 0.8521790696451106 1105 64-65 0.6206565581958117 1105 66-67 0.6671555966896605 1105 68-69 0.37650826409769067 1105 70-71 0.43224309926860727 1105 72-73 0.45362831000872106 1105 74-75 0.4036777732420198 1105 76-77 0.3513036038110542 1105 78-79 0.15990847930880747 1105 80-81 0.11932512048481314 1105 82-83 0.15369352173296136 1105 84-85 0.12689350368672336 1105 86-87 -0.007870750570162954 1105 88-89 0.23537966055520343 1105 90-91 0.07607047425984703 1105 92-93 0.06062114543465924 1105 94-95 0.373197794893958 1105 96-97 1.816908982312583 1105 98-99 1.9025274019734084 1105 100-101 1.2226666294053068 1106 1 0.9983910532940854 1106 2 1.0219259686948412 1106 3 1.0169791731869111 1106 4 1.0412050593234952 1106 5 0.8483197967934473 1106 6 1.0190775299662675 1106 7 1.0413155676493488 1106 8 1.0342312205357302 1106 9 1.2389532396177003 1106 10-11 1.128685929214818 1106 12-13 1.093203697891738 1106 14-15 1.3225098820225796 1106 16-17 1.4742254208579695 1106 18-19 1.325714175595131 1106 20-21 1.2471727799570758 1106 22-23 1.0242914362962097 1106 24-25 1.1352812977220026 1106 26-27 1.307625144034894 1106 28-29 1.247906003167408 1106 30-31 1.2311843314985111 1106 32-33 1.2733581165852854 1106 34-35 1.478597874003377 1106 36-37 1.4673688801772187 1106 38-39 1.4838566705888425 1106 40-41 1.542590210885571 1106 42-43 1.3865224860671574 1106 44-45 1.4953451275887986 1106 46-47 1.4378201666015968 1106 48-49 1.4303252206993768 1106 50-51 1.661411339379871 1106 52-53 1.70821267081649 1106 54-55 1.5026103847420522 1106 56-57 1.4633971225586215 1106 58-59 1.361154982796851 1106 60-61 1.6527303612812396 1106 62-63 1.63109286046803 1106 64-65 1.680545825124394 1106 66-67 1.5524208136926063 1106 68-69 1.4047879617784602 1106 70-71 1.7562584490227309 1106 72-73 1.9197557666783602 1106 74-75 1.7457434391799858 1106 76-77 1.7017330324508286 1106 78-79 1.507383499736406 1106 80-81 1.615873046813281 1106 82-83 1.5780603623402207 1106 84-85 1.531498112406446 1106 86-87 1.528788961647848 1106 88-89 1.5127525768422068 1106 90-91 1.388343476017301 1106 92-93 1.4933285616777567 1106 94-95 2.022267149622561 1106 96-97 3.5460701812184254 1106 98-99 3.7268045775698564 1106 100-101 2.957102112919136 1107 1 0.5827548225400783 1107 2 0.7198917887475638 1107 3 0.6962709827618738 1107 4 0.3301260092411127 1107 5 0.7464916834203947 1107 6 0.832083448424477 1107 7 0.694150351436484 1107 8 0.4776260152568099 1107 9 0.6411108138585035 1107 10-11 0.8230444713700606 1107 12-13 0.77199278844801 1107 14-15 0.9265181480965836 1107 16-17 0.8778702141158519 1107 18-19 0.6901272662879094 1107 20-21 0.7219873690880405 1107 22-23 0.7382388740696371 1107 24-25 0.8090432393080107 1107 26-27 1.0322826360485564 1107 28-29 1.06762046107292 1107 30-31 1.0048592153562836 1107 32-33 1.2170556885393893 1107 34-35 1.1586580848791002 1107 36-37 0.9561529744557689 1107 38-39 0.8676355925947306 1107 40-41 1.0362773509151424 1107 42-43 1.1259151230161493 1107 44-45 0.9650725151455575 1107 46-47 0.7964718755948397 1107 48-49 0.9715959502853373 1107 50-51 0.9935549552027965 1107 52-53 1.2364455555564682 1107 54-55 1.094742372534352 1107 56-57 0.8749008933428222 1107 58-59 0.8912512705732176 1107 60-61 0.9858696468044599 1107 62-63 0.9090339934187242 1107 64-65 0.7648466153869862 1107 66-67 0.8707810998051393 1107 68-69 0.627991001981723 1107 70-71 0.6704492544043958 1107 72-73 0.7630628606920524 1107 74-75 0.6614905014041277 1107 76-77 0.6646455829174798 1107 78-79 0.5199435616140811 1107 80-81 0.5569855128518242 1107 82-83 0.5842859667426872 1107 84-85 0.32651306208384767 1107 86-87 0.2948082719603846 1107 88-89 0.2433744011537584 1107 90-91 0.22549478656955557 1107 92-93 0.4224158338148065 1107 94-95 0.7134984637623099 1107 96-97 2.272620257629903 1107 98-99 2.3558654133718573 1107 100-101 2.0069047224417726 1108 1 0.4903850837433943 1108 2 0.47998256108727944 1108 3 0.5048941202383759 1108 4 0.5543643684203232 1108 5 0.7223916976647189 1108 6 0.5818164065007707 1108 7 0.4892996115926067 1108 8 0.5359633270869537 1108 9 0.5373846711429096 1108 10-11 0.5616763667572471 1108 12-13 0.4235714038058944 1108 14-15 0.48477688135363906 1108 16-17 0.5013000123004048 1108 18-19 0.568301011026513 1108 20-21 0.42637176388432607 1108 22-23 0.38024886544477 1108 24-25 0.532643773704919 1108 26-27 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1.7080089765431836 2311 90-91 1.9642836791982106 2311 92-93 1.9292428585685784 2311 94-95 2.4270412905075567 2311 96-97 3.895718884467705 2311 98-99 4.17656355337766 2311 100-101 3.6847481362459984 2312 1 0.0528757077495392 2312 2 -0.4605991405730947 2312 3 -0.6202269425942859 2312 4 -0.26527811368277554 2312 5 -1.8615319982470488 2312 6 -0.4611052431940976 2312 7 -0.10510449524272047 2312 8 0.3395871112080684 2312 9 0.37683885328621614 2312 10-11 0.11234333745614578 2312 12-13 0.2875423847298748 2312 14-15 0.38460991365506203 2312 16-17 -0.29423956489477376 2312 18-19 0.03183244535855323 2312 20-21 0.3993602006586201 2312 22-23 0.2693599612209283 2312 24-25 0.40338062181081824 2312 26-27 0.5037391287192108 2312 28-29 0.5538543708082315 2312 30-31 0.3559945287227535 2312 32-33 -0.3180428024946593 2312 34-35 0.26667574741594535 2312 36-37 0.5437145087803188 2312 38-39 0.7001085915272327 2312 40-41 0.8757314503794689 2312 42-43 0.791487110463855 2312 44-45 0.794297929780889 2312 46-47 0.4351808217383706 2312 48-49 0.7909643990259703 2312 50-51 0.6653510998894525 2312 52-53 0.49502165045889157 2312 54-55 0.568646582284206 2312 56-57 0.3646394794911778 2312 58-59 0.7300411213559563 2312 60-61 0.7843670987559968 2312 62-63 0.9022724406756417 2312 64-65 0.7765133081365541 2312 66-67 0.8220495412927207 2312 68-69 0.7501768327075133 2312 70-71 0.8878101801346467 2312 72-73 0.986517441385871 2312 74-75 1.1722265237038023 2312 76-77 1.132802312952549 2312 78-79 1.1745681047996896 2312 80-81 0.8877697694545859 2312 82-83 1.0856284962040696 2312 84-85 1.2277414236855293 2312 86-87 1.2039958051972732 2312 88-89 1.2350649537370337 2312 90-91 1.4500185153366942 2312 92-93 1.526514026600939 2312 94-95 1.9826970827329866 2312 96-97 3.53330101678673 2312 98-99 3.9768849623042275 2312 100-101 3.629050826905589 2313 1 0.03648896729484363 2313 2 -0.3814585774652599 2313 3 -0.8123863974693535 2313 4 -0.07923384601143368 2313 5 -0.19790917831650034 2313 6 0.2143824322285397 2313 7 0.14703243955025158 2313 8 0.4082850118972772 2313 9 0.44356821233157007 2313 10-11 -0.48804002855995066 2313 12-13 0.1826558242471492 2313 14-15 0.37598115850927627 2313 16-17 -0.2934901224647959 2313 18-19 0.17152095948771517 2313 20-21 -2.1370424885428037 2313 22-23 -0.1699106505722554 2313 24-25 0.2930150657288948 2313 26-27 0.4170308445481936 2313 28-29 0.23287962463445666 2313 30-31 0.5207582193859679 2313 32-33 0.7400873202379259 2313 34-35 0.4801499439533359 2313 36-37 -0.5095284959710042 2313 38-39 0.35873665337354765 2313 40-41 0.44368784227177827 2313 42-43 0.48554306101745226 2313 44-45 0.6297477682063786 2313 46-47 0.5352696426918442 2313 48-49 0.29228281160176905 2313 50-51 0.5173454580042183 2313 52-53 0.6847274327479944 2313 54-55 0.728988165928854 2313 56-57 0.6830720680541127 2313 58-59 0.7031144421346163 2313 60-61 0.4467489483760545 2313 62-63 0.5784788246343204 2313 64-65 0.4570340875366661 2313 66-67 0.616963196204555 2313 68-69 0.724435945735916 2313 70-71 0.9193961534141692 2313 72-73 0.7175233231384297 2313 74-75 0.8628735851004024 2313 76-77 1.0656753073515475 2313 78-79 0.8827690798789298 2313 80-81 0.9880055055685126 2313 82-83 0.9329562698951115 2313 84-85 0.8611027582169584 2313 86-87 0.7490701697847371 2313 88-89 1.1382046211305479 2313 90-91 1.394086330602832 2313 92-93 1.545714955364918 2313 94-95 2.1452777377623207 2313 96-97 3.45483106009722 2313 98-99 3.7097059663905494 2313 100-101 3.1964888166287473 2314 1 -0.30128892461058143 2314 2 -0.2406534042471229 2314 3 -1.235959223203043 2314 4 -0.234610070662427 2314 5 -0.4693409454759703 2314 6 -0.47110008186777463 2314 7 -0.2418203572539639 2314 8 -1.934587425318778 2314 9 -1.6235273218919914 2314 10-11 -0.8518429864240602 2314 12-13 -0.6819046558772044 2314 14-15 -0.6277546015290056 2314 16-17 -0.7284413727539416 2314 18-19 -1.8661494063021422 2314 20-21 -1.2998059661498615 2314 22-23 -1.9106482690021238 2314 24-25 -1.905231818380571 2314 26-27 -2.027289858405375 2314 28-29 -3.1477913250641016 2314 30-31 -1.6463559671830623 2314 32-33 -1.3836457012071222 2314 34-35 -1.6451771687334258 2314 36-37 -3.2728020841863312 2314 38-39 -3.546846923419686 2314 40-41 -4.158408282068535 2314 42-43 -2.9812052884369216 2314 44-45 -3.2940252668935415 2314 46-47 -3.216152404648568 2314 48-49 -1.5991342241553568 2314 50-51 -1.0713410479197734 2314 52-53 -2.4729484835960953 2314 54-55 -1.8709380006945793 2314 56-57 -1.849206824580687 2314 58-59 -3.9171089895428075 2314 60-61 -2.5138895586574783 2314 62-63 -2.276980771203778 2314 64-65 -1.6739431902522774 2314 66-67 -2.8222409158333974 2314 68-69 -2.652289793040527 2314 70-71 -2.0993091720491535 2314 72-73 -1.4941380470569428 2314 74-75 -1.9089178957864839 2314 76-77 -0.9855193492735168 2314 78-79 -0.6853228098710353 2314 80-81 -0.6001813234911388 2314 82-83 -0.49005991313095265 2314 84-85 -0.3969412244933608 2314 86-87 -0.1848525913041854 2314 88-89 0.008351951379566458 2314 90-91 0.492993531727393 2314 92-93 0.8170252071497117 2314 94-95 1.3639342666017455 2314 96-97 2.878306038617744 2314 98-99 3.0243290595603565 2314 100-101 2.626712536839051 2315 1 -2.1868665619222902 2315 2 -1.6678890792126673 2315 3 -1.484674856906846 2315 4 -1.5752343426169375 2315 5 -1.5816539496403337 2315 6 -2.9554223287860673 2315 7 -2.040578651390774 2315 8 -1.2824136559754038 2315 9 -1.037027061738243 2315 10-11 -3.423658606436298 2315 12-13 -1.8708039843577637 2315 14-15 -1.2160382808561607 2315 16-17 -2.0977626882662577 2315 18-19 -1.3715866864644894 2315 20-21 -1.0162425285424206 2315 22-23 -1.1725246796919038 2315 24-25 -2.909522509306594 2315 26-27 -4.261714581077399 2315 28-29 -5.7146405716800786 2315 30-31 -8.617877060381531 2315 32-33 -6.873605573748311 2315 34-35 -6.2398406098077075 2315 36-37 -3.9938408961440786 2315 38-39 -6.391150618165497 2315 40-41 -3.97410374909251 2315 42-43 -3.710007785856657 2315 44-45 -3.0690905831000705 2315 46-47 -2.282709009062337 2315 48-49 -2.8700911223153796 2315 50-51 -2.75987280682423 2315 52-53 -2.0193209684923055 2315 54-55 -3.469775715738077 2315 56-57 -3.3721060153720828 2315 58-59 -2.4172960794332248 2315 60-61 -2.5488047303923587 2315 62-63 -3.0316596182743716 2315 64-65 -2.331671922498689 2315 66-67 -2.8850500264113634 2315 68-69 -2.160159347260276 2315 70-71 -2.711853884985892 2315 72-73 -1.93223755834482 2315 74-75 -2.3043065536737153 2315 76-77 -1.5320941486274293 2315 78-79 -1.2796236038621025 2315 80-81 -0.9933348180004842 2315 82-83 -0.7939354902317142 2315 84-85 -0.7139701948407549 2315 86-87 -1.204801661786334 2315 88-89 -1.764840110158346 2315 90-91 -1.5042268420206142 2315 92-93 -0.5494388825262959 2315 94-95 0.0434253399070208 2315 96-97 1.4616838912111625 2315 98-99 1.387884448188709 2315 100-101 1.2831299873013666 2316 1 -3.3433535828609884 2316 2 -2.0869058223920547 2316 3 -2.2982731724321965 2316 4 -2.89296546824448 2316 5 -2.417693670485427 2316 6 -5.7770728002965654 2316 7 -3.5193170535899476 2316 8 -4.494968832385656 2316 9 -6.287497268851222 2316 10-11 -5.603126898194951 2316 12-13 -3.5568769103629094 2316 14-15 -4.898395827114424 2316 16-17 -4.495524593229547 2316 18-19 -2.530401569725072 2316 20-21 -3.172848310185099 2316 22-23 -4.022442209291775 2316 24-25 -2.784362523801917 2316 26-27 -3.747205843083549 2316 28-29 -3.6980552545935055 2316 30-31 -3.3944091410586665 2316 32-33 -3.241591022684915 2316 34-35 -5.079755721902767 2316 36-37 -5.309766727345952 2316 38-39 -5.0888671888472174 2316 40-41 -4.583072795948773 2316 42-43 -3.942254603184182 2316 44-45 -3.358896997606262 2316 46-47 -3.631752125714815 2316 48-49 -4.11926920880936 2316 50-51 -5.342629605354563 2316 52-53 -3.2015866883918918 2316 54-55 -3.485972963332234 2316 56-57 -2.78626060658949 2316 58-59 -3.1339186539642334 2316 60-61 -3.20698171684192 2316 62-63 -2.4226061286058247 2316 64-65 -2.661472719963296 2316 66-67 -3.187717330813072 2316 68-69 -2.5521683603172285 2316 70-71 -1.9980696080850144 2316 72-73 -2.1803386523368076 2316 74-75 -3.303817818365676 2316 76-77 -3.3666806627498005 2316 78-79 -3.257855903820168 2316 80-81 -3.6035809757312016 2316 82-83 -2.3615270737653127 2316 84-85 -2.223003530547455 2316 86-87 -1.8753964560186844 2316 88-89 -2.097822106853709 2316 90-91 -1.7320343107447087 2316 92-93 -1.4820360473970027 2316 94-95 -1.260843639429659 2316 96-97 0.3338481847551762 2316 98-99 0.8378922927734447 2316 100-101 0.6102493788305896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29412.0 3 9389.0 4 9322.0 5 10328.0 6 11653.0 7 13878.0 8 16871.0 9 20273.0 10 24214.0 11 28021.0 12 32689.0 13 36577.0 14 39516.0 15 43182.0 16 47983.0 17 51788.0 18 48839.0 19 49117.0 20 53451.0 21 58154.0 22 64609.0 23 72500.0 24 81511.0 25 91673.0 26 103252.0 27 116731.0 28 130965.0 29 148248.0 30 167762.0 31 190543.0 32 217657.0 33 249005.0 34 284933.0 35 314849.0 36 313914.0 37 232451.0 38 54595.0 39 453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.102892911052336 18.349466609427004 12.772811621498109 23.774828858022552 2 16.147889107690673 20.042124953192893 39.48131091054174 24.328675028574693 3 19.779722629283047 24.196118587277255 29.122800032199404 26.901358751240295 4 13.00709216992928 15.74423056001796 36.03657443892732 35.21210283112544 5 13.873976922393267 37.48890499676239 33.750538158154434 14.886579922689902 6 32.966790389818826 35.21559309924332 17.338458470890338 14.479158040047519 7 29.53210636401834 32.32401178333803 20.176657724504448 17.967224128139183 8 27.040024056046896 34.86337602492872 19.426325614870656 18.670274304153736 9 27.483688161045066 16.031576626393086 19.518015750794813 36.966719461767035 10-11 25.76435861203607 26.254261507314276 26.94862729214515 21.03275258850451 12-13 26.23813857409366 23.571531740925376 27.560309785532983 22.63001989944798 14-15 23.116049430451582 24.79343743566223 25.62704573368538 26.463467400200802 16-17 22.99684893045428 28.324464938580114 25.973628548920928 22.705057582044674 18-19 22.797586510038794 26.841951387329583 28.23166201904955 22.128800083582068 20-21 23.523003504801057 26.05314363910365 28.34239365786906 22.081459198226234 22-23 23.179719982796513 26.05316131386935 28.145689101701105 22.621429601633032 24-25 22.902140137126924 26.192308375439723 28.311796031749353 22.593755455683997 26-27 22.759979581271665 26.573978540118688 28.248444227196618 22.41759765141303 28-29 22.494726640936342 26.619390914212083 28.49664464614161 22.38923779870996 30-31 22.818225757097245 26.269364350663103 28.42263638927506 22.489773502964592 32-33 22.615963647291387 26.50495947837496 28.327057118155114 22.552019756178534 34-35 22.8689322273522 26.36287266554167 28.20422239870209 22.56397270840404 36-37 22.91866620609596 26.331099623147114 28.136982350547523 22.613251820209403 38-39 22.550746079469974 26.579797544069063 28.19491472325662 22.67454165320435 40-41 22.856809200573707 26.290467303965837 28.307278975825284 22.545444519635176 42-43 22.685903688531393 26.39724326967203 28.18950485590639 22.727348185890193 44-45 22.53516633225105 26.387103005063224 28.139562111150713 22.938168551535014 46-47 22.62543886184344 26.62014833403965 27.83439954891897 22.920013255197937 48-49 22.75944705864853 26.671351582263807 27.663459001428436 22.90574235765922 50-51 22.45229878275394 26.73213783524532 27.675249676777568 23.140313705223168 52-53 22.732465132379144 26.783883378699514 27.54421775881874 22.939433730102603 54-55 22.34501295531399 26.531162672978287 27.72333181552373 23.400492556184 56-57 22.341511262048066 26.908645898425736 27.5628927116388 23.186950127887396 58-59 22.649294881968167 26.46122258774859 27.509993329837716 23.379489200445526 60-61 22.569980891825498 26.47828475439102 27.910004612815833 23.04172974096765 62-63 22.35929413235502 26.539518214251117 27.70648450466658 23.39470314872728 64-65 22.143184301326126 26.63237589491451 27.755128495123216 23.469311308636147 66-67 22.258927416672826 26.689797386556798 27.430948493274705 23.620326703495675 68-69 21.968802643374026 26.49068713000775 27.740183702369247 23.800326524248973 70-71 21.948052810444988 26.574784337717073 27.703671260783114 23.773491591054828 72-73 22.26557603561524 26.418073567629737 27.47833978080707 23.83801061594795 74-75 21.9235150947244 26.758783663947767 27.330529893949574 23.98717134737826 76-77 22.125990018667512 26.540813492031965 27.305743708075564 24.02745278122496 78-79 22.272015210685147 26.58131936285902 27.363027716690013 23.783637709765824 80-81 22.240853810466337 26.797189536696486 27.325404452235396 23.63655220060178 82-83 22.289180815645604 26.56485660843378 27.30904836531448 23.836914210606135 84-85 22.34098369273477 26.398426304207895 27.10689846551374 24.153691537543594 86-87 22.238111411277675 26.248714087592802 27.442439354897964 24.070735146231563 88-89 22.310315408360363 26.262522989621168 27.36533165127864 24.061829950739828 90-91 22.480535300008736 26.34319246351655 27.31757191451201 23.858700321962708 92-93 22.534624426536773 25.920783436773103 27.297672872282806 24.24691926440731 94-95 22.459204813040596 25.837380211258004 27.266771579304944 24.43664339639646 96-97 22.443210542745515 25.79971515557525 27.215924156741096 24.541150144938143 98-99 22.644124328730065 25.596743798882038 26.98388085067026 24.775251021717644 100-101 22.661738500902388 25.76697145154793 27.11315967971066 24.458130367839022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4227.0 1 2461.0 2 671.0 3 641.0 4 572.5 5 570.0 6 787.5 7 1305.5 8 2037.0 9 2440.0 10 2298.5 11 2142.5 12 2287.5 13 2569.5 14 2872.0 15 3224.5 16 3543.0 17 3818.0 18 4222.5 19 4762.0 20 5460.0 21 6227.0 22 7266.0 23 8743.0 24 10695.0 25 13218.0 26 16534.5 27 20471.5 28 24246.5 29 28178.0 30 33137.5 31 38795.0 32 45406.5 33 51983.0 34 58925.0 35 65844.5 36 72545.5 37 79724.5 38 85236.5 39 89554.0 40 94302.0 41 100819.5 42 107482.5 43 115463.5 44 123509.0 45 129455.5 46 133004.0 47 135568.5 48 141034.5 49 143751.0 50 141297.0 51 137117.5 52 133239.0 53 129104.5 54 122610.0 55 112523.0 56 100332.0 57 90830.5 58 83949.0 59 76760.5 60 67450.5 61 55190.0 62 42881.5 63 33153.0 64 26298.5 65 21101.5 66 16328.0 67 12883.0 68 9591.5 69 6345.5 70 4591.0 71 3608.0 72 2937.5 73 2304.5 74 1768.5 75 1404.5 76 939.5 77 591.5 78 411.0 79 268.5 80 175.5 81 123.5 82 82.5 83 54.5 84 36.5 85 25.0 86 17.5 87 12.5 88 9.5 89 6.5 90 5.5 91 4.5 92 3.5 93 2.5 94 3.0 95 4.5 96 3.5 97 3.5 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12045040382582756 2 0.11393801357112972 3 0.1269339781944427 4 0.1422640295904571 5 0.1378551990197988 6 0.13759585604505423 7 0.1462694377559571 8 0.1460100947812125 9 0.15387683168179886 10-11 0.14786871943354885 12-13 0.1480127988639625 14-15 0.13576604727880062 16-17 0.13883493914661177 18-19 0.1570465791508996 20-21 0.18695746890477732 22-23 0.2033393001428115 24-25 0.2233087091981461 26-27 0.22477831938836554 28-29 0.2263920090089986 30-31 0.2263920090089986 32-33 0.22757346033839074 34-35 0.19875757425565685 36-37 0.1870006927339014 38-39 0.19989580175592483 40-41 0.18799484080375575 42-43 0.1913230756463115 44-45 0.19604888096387985 46-47 0.21656579185478636 48-49 0.23434519356783315 50-51 0.2501939309133368 52-53 0.27076847357640876 54-55 0.2645298342394969 56-57 0.23290439926369647 58-59 0.2268818790724051 60-61 0.20547167571293382 62-63 0.19587598564738345 64-65 0.18728885159472874 66-67 0.1836148261191802 68-69 0.188830501500155 70-71 0.19234603960224855 72-73 0.22966261207938893 74-75 0.24118896651248245 76-77 0.25780132483917856 78-79 0.24582832417180261 80-81 0.25195169996438355 82-83 0.24876754455224148 84-85 0.2345469047704123 86-87 0.2243604890401659 88-89 0.22695391878761192 90-91 0.22910070230077562 92-93 0.23712592657481696 94-95 0.2414771253733098 96-97 8.659389887007148 98-99 0.3010683777924034 100-101 0.3133295373206067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3470308.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.45439591130341 #Duplication Level Percentage of deduplicated Percentage of total 1 75.59940031774434 38.143220742885894 2 12.297594968450367 12.409354505900952 3 4.666152726211844 7.062837510927003 4 2.3270401056090178 4.696376111595146 5 1.325647641648515 3.344237547530992 6 0.830517197885345 2.514194610795211 7 0.5383086509469093 1.9012026458148497 8 0.3971011984273767 1.6028400865846333 9 0.29081176673433706 1.3205458813031872 >10 1.5357907734194194 14.251797002586923 >50 0.12275143742945038 4.278834655272722 >100 0.06468751942824903 6.0871805468941025 >500 0.003088498494171224 1.0629549922768142 >1k 9.906504578857302E-4 0.8515576959642933 >5k 1.1654711269243884E-4 0.47286546366722126 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9078 0.261590613859058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7005 0.20185528200955075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4294 0.12373541483925922 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3511 0.10117257603647861 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.8815886082733867E-5 0.0 4 0.0 0.0 0.0 2.8815886082733867E-5 0.0 5 0.0 0.0 0.0 2.8815886082733867E-5 0.0 6 0.0 0.0 0.0 2.8815886082733867E-5 0.0 7 0.0 0.0 0.0 2.8815886082733867E-5 0.0 8 0.0 0.0 0.0 2.8815886082733867E-5 0.0 9 0.0 0.0 0.0 5.763177216546773E-5 0.0 10-11 0.0 0.0 0.0 5.763177216546773E-5 2.8815886082733867E-5 12-13 0.0 0.0 0.0 5.763177216546773E-5 2.8815886082733867E-5 14-15 0.0 0.0 0.0 7.203971520683467E-5 2.8815886082733867E-5 16-17 0.0 0.0 0.0 8.64476582482016E-5 2.8815886082733867E-5 18-19 0.0 0.0 0.0 8.64476582482016E-5 2.8815886082733867E-5 20-21 0.0 0.0 0.0 1.8730325953777012E-4 2.8815886082733867E-5 22-23 0.0 0.0 0.0 3.1697474691007256E-4 2.8815886082733867E-5 24-25 0.0 0.0 0.0 8.932924685647499E-4 2.8815886082733867E-5 26-27 0.0 0.0 0.0 0.0022332311714118747 2.8815886082733867E-5 28-29 0.0 0.0 0.0 0.0071895635776421 2.8815886082733867E-5 30-31 0.0 0.0 0.0 0.026438575480908326 2.8815886082733867E-5 32-33 0.0 0.0 0.0 0.07077181621919437 2.8815886082733867E-5 34-35 0.0 0.0 0.0 0.14382008743892472 2.8815886082733867E-5 36-37 0.0 0.0 0.0 0.27207959639317314 2.8815886082733867E-5 38-39 0.0 1.4407943041366933E-5 0.0 0.46099654555157643 2.8815886082733867E-5 40-41 0.0 2.8815886082733867E-5 0.0 0.6696379687336109 2.8815886082733867E-5 42-43 0.0 2.8815886082733867E-5 0.0 0.8736688501424081 2.8815886082733867E-5 44-45 0.0 2.8815886082733867E-5 0.0 1.118877632763432 2.8815886082733867E-5 46-47 0.0 2.8815886082733867E-5 0.0 1.4106961111232779 2.8815886082733867E-5 48-49 0.0 2.8815886082733867E-5 0.0 1.7209999804051974 2.8815886082733867E-5 50-51 0.0 2.8815886082733867E-5 0.0 2.053751424945567 2.8815886082733867E-5 52-53 0.0 2.8815886082733867E-5 0.0 2.3735645366347886 2.8815886082733867E-5 54-55 0.0 2.8815886082733867E-5 0.0 2.7028004430730643 2.8815886082733867E-5 56-57 0.0 2.8815886082733867E-5 0.0 3.0868585727837416 2.8815886082733867E-5 58-59 1.4407943041366933E-5 2.8815886082733867E-5 0.0 3.508478210003262 2.8815886082733867E-5 60-61 2.8815886082733867E-5 2.8815886082733867E-5 0.0 3.948885804948725 2.8815886082733867E-5 62-63 2.8815886082733867E-5 2.8815886082733867E-5 0.0 4.3769746085938195 2.8815886082733867E-5 64-65 2.8815886082733867E-5 2.8815886082733867E-5 0.0 4.805423610814948 2.8815886082733867E-5 66-67 2.8815886082733867E-5 2.8815886082733867E-5 0.0 5.28113066621176 2.8815886082733867E-5 68-69 4.32238291241008E-5 2.8815886082733867E-5 0.0 5.807640128772432 2.8815886082733867E-5 70-71 5.763177216546773E-5 2.8815886082733867E-5 0.0 6.365558907163283 2.8815886082733867E-5 72-73 5.763177216546773E-5 2.8815886082733867E-5 0.0 6.909963034981333 2.8815886082733867E-5 74-75 5.763177216546773E-5 2.8815886082733867E-5 0.0 7.434354529914923 2.8815886082733867E-5 76-77 5.763177216546773E-5 2.8815886082733867E-5 0.0 7.994362460046774 2.8815886082733867E-5 78-79 5.763177216546773E-5 2.8815886082733867E-5 0.0 8.607492476172144 2.8815886082733867E-5 80-81 5.763177216546773E-5 2.8815886082733867E-5 0.0 9.250129959646234 2.8815886082733867E-5 82-83 5.763177216546773E-5 2.8815886082733867E-5 0.0 9.888041637802754 2.8815886082733867E-5 84-85 5.763177216546773E-5 2.8815886082733867E-5 0.0 10.495451700540702 2.8815886082733867E-5 86-87 5.763177216546773E-5 2.8815886082733867E-5 0.0 11.037795492503836 2.8815886082733867E-5 88-89 5.763177216546773E-5 2.8815886082733867E-5 0.0 11.590527411399796 2.8815886082733867E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7820 0.0 40.239506 1 GTATCAA 12635 0.0 36.594143 1 ATCAACG 15435 0.0 29.593052 3 TATCAAC 15710 0.0 29.19312 2 TCAACGC 15635 0.0 29.12466 4 TACATGG 15835 0.0 28.606209 2 CAACGCA 15975 0.0 28.535477 5 GTACATG 16595 0.0 28.457039 1 AACGCAG 16375 0.0 28.01161 6 ACATGGG 16090 0.0 27.248148 3 ACGCAGA 19220 0.0 23.69391 7 CGCAGAG 19470 0.0 23.196037 8 CATGGGG 7920 0.0 23.164497 4 CATGGGA 8970 0.0 21.449358 4 GCAGAGT 20040 0.0 21.22196 9 GAGTACT 9245 0.0 20.811478 12-13 GTACTTT 9855 0.0 19.361122 14-15 AGTACTT 9660 0.0 19.089542 12-13 AGAGTAC 17140 0.0 18.732023 10-11 CAGAGTA 19370 0.0 18.457666 10-11 >>END_MODULE