FastQCFastQC Report
Fri 27 May 2016
SRR939840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470308
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99960.28804359728300777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87730.25280176860382425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53730.15482775592252906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79650.044.3297651
GTATCAA123700.035.334441
ATCAACG155600.027.80313
TCAACGC156100.027.7140464
TATCAAC157950.027.4858762
GTACATG169000.027.3795411
CAACGCA158600.027.2771915
TACATGG163450.027.2576752
AACGCAG164750.026.3453316
ACATGGG166550.025.8042663
TGCACCG11000.024.1492315
CATGGGG81250.022.7692784
ACGCAGA194050.022.09857
CGCAGAG196500.021.678138
GAGTACT98400.021.4525612-13
GTACTTT103600.020.33000414-15
GCAGAGT206850.020.2265159
GAGTACA134450.020.0491431
GCACCGT13500.020.0285326
AGTACAT124850.020.0276582
Produced by FastQC (version 0.11.5)