FastQCFastQC Report
Fri 27 May 2016
SRR939839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004231
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86130.1721143568312494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62720.12533394241792595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.048.4176751
GTATCAA110700.043.581581
ATCAACG145550.033.7997553
TCAACGC145900.033.75224
TATCAAC148750.033.070652
CAACGCA150400.032.553725
AACGCAG156200.031.3145646
TACATGG220900.031.181132
GTACATG216850.030.8453221
ACATGGG231700.028.6648063
ACGCAGA186400.026.1647137
CATGGGA121400.025.6357024
CGCAGAG190250.025.5100358
GAGTACA152650.023.4938721
CATGGGG117050.022.737964
AGTACAT154000.022.3633482
GCAGAGT212800.021.7364549
ATGGGAT52500.020.6036575
ATGGGAG54900.020.3942815
GTGGTAT36700.019.0011941
Produced by FastQC (version 0.11.5)