##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5004231 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.796810738752868 31.0 28.0 33.0 16.0 34.0 2 29.624007964460475 31.0 30.0 33.0 25.0 34.0 3 29.899345973437278 31.0 30.0 33.0 25.0 34.0 4 33.67912692279793 35.0 33.0 37.0 28.0 37.0 5 33.62416283341037 35.0 33.0 37.0 28.0 37.0 6 33.63935757561951 35.0 33.0 37.0 28.0 37.0 7 33.53639450297158 35.0 33.0 37.0 28.0 37.0 8 33.396863174381835 35.0 33.0 37.0 27.0 37.0 9 34.665531027644406 37.0 34.0 39.0 27.0 39.0 10-11 34.48972799217302 37.0 33.5 39.0 27.0 39.0 12-13 34.36206961669036 37.0 33.0 39.0 27.0 39.0 14-15 35.03203709021426 38.0 33.0 40.0 26.0 41.0 16-17 34.838213703564044 38.0 33.0 40.0 25.5 41.0 18-19 34.62356843638913 38.0 33.0 40.0 25.0 41.0 20-21 34.773815597241615 38.0 33.0 40.0 25.0 41.0 22-23 34.658798924350215 38.0 33.0 40.0 24.5 41.0 24-25 34.42471041005102 38.0 33.0 40.0 24.0 41.0 26-27 34.31219591981265 38.0 32.5 40.0 23.5 41.0 28-29 34.393696454060574 38.0 33.0 40.0 24.0 41.0 30-31 34.186601297981646 38.0 33.0 40.0 23.5 41.0 32-33 33.85542703764075 38.0 32.5 40.0 21.0 41.0 34-35 33.684364390852465 38.0 32.0 40.0 20.5 41.0 36-37 33.49134872071254 38.0 31.5 40.0 19.0 41.0 38-39 33.26117059744044 37.0 31.0 40.0 18.5 41.0 40-41 33.024359686833 37.0 31.0 40.0 17.0 41.0 42-43 32.63556518474067 37.0 31.0 40.0 15.0 41.0 44-45 32.31180784819885 37.0 30.0 40.0 14.0 41.0 46-47 31.941588727618687 36.5 30.0 39.0 10.5 41.0 48-49 31.5898325836677 36.0 30.0 39.0 9.0 40.0 50-51 30.94139479172724 35.5 28.5 38.5 8.0 40.0 52-53 31.08545988384629 35.5 29.0 39.0 8.0 40.0 54-55 31.17551427981642 36.0 29.0 39.0 7.0 40.0 56-57 30.842637859843002 35.0 28.5 39.0 4.0 40.0 58-59 30.418544327789824 35.0 28.0 39.0 2.0 40.0 60-61 29.986752510026015 34.5 28.0 38.0 2.0 40.0 62-63 29.50622283423767 34.0 27.0 38.0 2.0 40.0 64-65 28.93591812608171 34.0 26.0 37.5 2.0 40.0 66-67 28.328096564686962 33.5 26.0 37.0 2.0 39.0 68-69 27.785733612217342 33.0 25.0 36.0 2.0 39.0 70-71 27.19852340949089 32.5 24.0 36.0 2.0 38.5 72-73 26.523953830268827 32.0 22.0 35.0 2.0 38.0 74-75 26.156094812569606 32.0 21.5 35.0 2.0 37.5 76-77 25.592449069597308 31.0 20.0 35.0 2.0 37.0 78-79 24.93243667208808 31.0 18.0 34.5 2.0 36.0 80-81 24.199586909557134 30.5 12.5 34.0 2.0 36.0 82-83 23.466730952268193 30.0 7.0 34.0 2.0 35.0 84-85 22.7113749345304 29.5 2.0 34.0 2.0 35.0 86-87 21.854876503502737 29.0 2.0 34.0 2.0 35.0 88-89 20.912267739039223 28.0 2.0 33.5 2.0 35.0 90-91 19.511071831016594 26.0 2.0 32.5 2.0 34.5 92-93 17.952861388692888 22.0 2.0 32.0 2.0 34.0 94-95 17.386264742774664 20.0 2.0 32.0 2.0 34.0 96-97 16.96068476854885 19.5 2.0 32.0 2.0 34.0 98-99 15.997037906523499 9.0 2.0 31.5 2.0 34.0 100-101 14.350274098058224 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4621882619372606 1101 2 0.8656842254340944 1101 3 0.8433000463652647 1101 4 0.6927282954044927 1101 5 0.8435883271552029 1101 6 0.8820123817623369 1101 7 0.7462755509226184 1101 8 0.9668724083393698 1101 9 0.8346964316558712 1101 10-11 0.927475565014312 1101 12-13 0.9000460419736385 1101 14-15 0.7607919528747544 1101 16-17 0.7880234585286132 1101 18-19 1.0886077397393947 1101 20-21 0.9455456550392327 1101 22-23 0.9930173119184218 1101 24-25 1.3703437828482308 1101 26-27 1.3633828907118826 1101 28-29 1.0052387349065057 1101 30-31 1.1330015703157414 1101 32-33 1.2369419258369803 1101 34-35 1.1314133384481195 1101 36-37 1.040317901555369 1101 38-39 1.104296330912895 1101 40-41 1.1501238379609333 1101 42-43 1.1288582308383397 1101 44-45 1.2554548433581232 1101 46-47 0.8100223346595286 1101 48-49 0.9775261219288573 1101 50-51 0.7943979426405363 1101 52-53 0.6479089682396477 1101 54-55 0.8593126814371068 1101 56-57 0.9608876557849229 1101 58-59 0.7385761562992776 1101 60-61 0.7584533382350145 1101 62-63 0.4276914488580026 1101 64-65 0.48001498884597993 1101 66-67 0.3567018320383255 1101 68-69 -0.031999767543521074 1101 70-71 0.16578310022653753 1101 72-73 -0.007307024676851626 1101 74-75 -0.16435694597391404 1101 76-77 -0.4352223540313638 1101 78-79 -0.8580660147884913 1101 80-81 -0.860347180215765 1101 82-83 -0.9092997142278918 1101 84-85 -0.8700870215892493 1101 86-87 -1.7795665383653052 1101 88-89 -5.440856123922824 1101 90-91 -8.703073459677679 1101 92-93 -10.67449706323942 1101 94-95 -8.862666151124838 1101 96-97 -5.905408881275367 1101 98-99 -4.519082159879995 1101 100-101 -3.3561679791229526 1102 1 1.8499908240829548 1102 2 1.1691515334290692 1102 3 1.0279841508466454 1102 4 1.0938070662397479 1102 5 1.005801318136612 1102 6 1.0546374303890573 1102 7 0.998674592939075 1102 8 1.2555065245505546 1102 9 1.449136081068346 1102 10-11 1.2750189133241818 1102 12-13 1.2557992327774699 1102 14-15 1.5285123098765538 1102 16-17 1.5427148236106163 1102 18-19 1.4706469217414124 1102 20-21 1.5920515652275284 1102 22-23 1.508120177851211 1102 24-25 1.6205718830059155 1102 26-27 1.629041437582103 1102 28-29 1.3573661725529362 1102 30-31 1.678471360129521 1102 32-33 1.796872968690863 1102 34-35 1.79782149778935 1102 36-37 1.7394710542002159 1102 38-39 1.6076881375700793 1102 40-41 1.627270763415794 1102 42-43 1.5652794414432663 1102 44-45 1.5777876490977079 1102 46-47 1.631531084499919 1102 48-49 1.5633139602000945 1102 50-51 1.612019988288779 1102 52-53 1.68552851202449 1102 54-55 1.536901831664725 1102 56-57 1.6210540556851498 1102 58-59 1.4718120010976286 1102 60-61 1.5221938207605774 1102 62-63 1.539305180450377 1102 64-65 1.3707870290005175 1102 66-67 1.3469890155191955 1102 68-69 1.356270038008983 1102 70-71 1.4086811860411963 1102 72-73 1.2913879046325114 1102 74-75 1.1740106141376216 1102 76-77 1.025830240037216 1102 78-79 0.957862911503792 1102 80-81 0.9415442136710759 1102 82-83 0.646905166855646 1102 84-85 0.7369418782738499 1102 86-87 0.7202783943590703 1102 88-89 0.13821640116262301 1102 90-91 -5.710335266691212 1102 92-93 -10.347034231130046 1102 94-95 -8.188543882016392 1102 96-97 -4.851532607700687 1102 98-99 -3.3028473863543137 1102 100-101 -2.5014454149876464 1103 1 2.104365741410465 1103 2 1.298145048726525 1103 3 0.9098558401297723 1103 4 0.8563881982293395 1103 5 0.911178391286569 1103 6 0.8150600626175617 1103 7 0.7799954083849627 1103 8 0.8758262157760726 1103 9 0.9896390544333471 1103 10-11 0.914575933518563 1103 12-13 0.791439557726271 1103 14-15 0.9693203546659888 1103 16-17 0.7565535521614777 1103 18-19 0.76156517141807 1103 20-21 0.7851347820489494 1103 22-23 0.859846245582375 1103 24-25 0.7851658354750839 1103 26-27 0.8398504311442565 1103 28-29 0.6616861715020903 1103 30-31 0.8308847526169671 1103 32-33 0.8128073094356338 1103 34-35 0.9036126466435022 1103 36-37 0.9838784615549443 1103 38-39 0.7904322836047726 1103 40-41 0.6536066525509554 1103 42-43 0.6942503878306638 1103 44-45 0.6334849581390003 1103 46-47 0.7726075215287267 1103 48-49 0.7655127577017957 1103 50-51 0.6385368679510179 1103 52-53 0.6109807247942136 1103 54-55 0.5743478994996316 1103 56-57 0.5350940763238796 1103 58-59 0.5392497868327872 1103 60-61 0.6191501721120289 1103 62-63 0.6288643048452727 1103 64-65 0.4624622972316992 1103 66-67 0.3564488818894951 1103 68-69 0.4411628990632295 1103 70-71 0.3167789737190816 1103 72-73 0.30367279614654663 1103 74-75 0.2254304767697235 1103 76-77 -0.052797416285851995 1103 78-79 -0.1942521279409739 1103 80-81 -0.35364127515980215 1103 82-83 -0.5051408899520808 1103 84-85 -0.6839943562513859 1103 86-87 -0.607378861581072 1103 88-89 -1.3280762100481383 1103 90-91 -6.91126365836333 1103 92-93 -10.041418684711005 1103 94-95 -8.787332937630286 1103 96-97 -5.6748632425258485 1103 98-99 -4.188053354954622 1103 100-101 -3.334392622646634 1104 1 1.9166453463549722 1104 2 1.2450672518744383 1104 3 1.084284930413105 1104 4 0.8937574981705296 1104 5 0.8549738142419656 1104 6 0.8446640442462439 1104 7 0.9146296131816172 1104 8 1.0631490685663962 1104 9 1.1789801036934904 1104 10-11 1.0122804930133142 1104 12-13 0.8870711455967779 1104 14-15 1.081566327847348 1104 16-17 1.0294187727253075 1104 18-19 1.006695651104529 1104 20-21 0.9540744934800003 1104 22-23 0.9260733496586582 1104 24-25 1.0334730016058842 1104 26-27 0.913323824880564 1104 28-29 0.876586615887561 1104 30-31 1.2235392209142617 1104 32-33 1.0811710296870984 1104 34-35 1.2089658560969312 1104 36-37 1.0840817491397345 1104 38-39 1.0916489242639855 1104 40-41 0.998478764283405 1104 42-43 1.0953640839011243 1104 44-45 1.0008675690785793 1104 46-47 1.0115561118033796 1104 48-49 0.8833390928112088 1104 50-51 0.8911774845837286 1104 52-53 0.9143630097353252 1104 54-55 0.8575533611584412 1104 56-57 0.9930479047383756 1104 58-59 0.9271839630482575 1104 60-61 0.9012727934623399 1104 62-63 0.9451621351823292 1104 64-65 0.8386407442566934 1104 66-67 0.9446677653687345 1104 68-69 0.761871846165942 1104 70-71 0.6556350294094457 1104 72-73 0.6214323873215086 1104 74-75 0.3648982119691482 1104 76-77 0.42009086252873473 1104 78-79 0.28638024949112406 1104 80-81 0.060736419859551916 1104 82-83 -0.25436072097790685 1104 84-85 -0.1803667331210157 1104 86-87 -0.13963121738506956 1104 88-89 -0.442067666650118 1104 90-91 -3.261959304641479 1104 92-93 -7.8374159889377 1104 94-95 -5.715559989965394 1104 96-97 -3.242050991988979 1104 98-99 -2.508123948955655 1104 100-101 -1.8270492819501154 1105 1 1.8702162432369285 1105 2 1.2192642000795892 1105 3 1.030447823555619 1105 4 0.8591072096448116 1105 5 0.8437324340316437 1105 6 0.8289680764232159 1105 7 0.8183950741693238 1105 8 0.8221420490859401 1105 9 0.7833229356957077 1105 10-11 0.7603582976543635 1105 12-13 0.79355720169319 1105 14-15 0.9943442202485429 1105 16-17 0.7719154928956016 1105 18-19 0.9120404866344316 1105 20-21 0.7698625054061239 1105 22-23 0.8425299435002884 1105 24-25 0.9446124534251013 1105 26-27 0.6759858241950596 1105 28-29 0.7036822076434888 1105 30-31 0.9344874281201427 1105 32-33 1.0073596904608024 1105 34-35 1.0153840347559324 1105 36-37 1.0127037024782126 1105 38-39 0.909844947348482 1105 40-41 0.7656965148819666 1105 42-43 0.8304193771332891 1105 44-45 0.8342648700135697 1105 46-47 0.9481303514997919 1105 48-49 0.7248957821895452 1105 50-51 0.8205673535081033 1105 52-53 0.6997828593259925 1105 54-55 0.6889184098670889 1105 56-57 0.6297480454582214 1105 58-59 0.5412481711244652 1105 60-61 0.5775321717893256 1105 62-63 0.5569809206483463 1105 64-65 0.5370033765650994 1105 66-67 0.536055122588234 1105 68-69 0.5003229896483283 1105 70-71 0.3897115194173537 1105 72-73 0.5107254510965831 1105 74-75 0.42904351267692675 1105 76-77 0.3970448536790627 1105 78-79 0.1493099045199635 1105 80-81 0.17069444435485437 1105 82-83 -0.07330700641643517 1105 84-85 -0.05131396102907004 1105 86-87 0.20888908225267144 1105 88-89 -0.32552192398759416 1105 90-91 -3.253430682113649 1105 92-93 -7.941035533028529 1105 94-95 -5.609958395026332 1105 96-97 -2.9332869879635872 1105 98-99 -1.9800792791264463 1105 100-101 -1.5545656940342258 1106 1 1.721521922415814 1106 2 1.178180769674725 1106 3 0.8929905831138107 1106 4 0.7542107470584227 1106 5 0.9175192879945371 1106 6 0.8597702505264166 1106 7 0.9076785201110695 1106 8 -0.1663187780919415 1106 9 -0.327331511614382 1106 10-11 -0.5275674246322382 1106 12-13 -0.375061518536711 1106 14-15 0.596091670884789 1106 16-17 0.7635772439674327 1106 18-19 0.9722003126213608 1106 20-21 0.7834823766294292 1106 22-23 0.8282429038900077 1106 24-25 0.9114201482187525 1106 26-27 0.8400212662285256 1106 28-29 0.7837368273273313 1106 30-31 0.9054096643953358 1106 32-33 0.9333957119669449 1106 34-35 0.9947054848893728 1106 36-37 0.9198138444938735 1106 38-39 0.963568997238589 1106 40-41 0.8677184912100415 1106 42-43 0.8237453484585657 1106 44-45 0.7900297158406033 1106 46-47 0.8146233326828352 1106 48-49 0.6467671853351327 1106 50-51 0.609000858610294 1106 52-53 0.7276434449685674 1106 54-55 0.7461504715481091 1106 56-57 0.8446846368335414 1106 58-59 0.7914024490988574 1106 60-61 0.6344417718084117 1106 62-63 0.7747372929617846 1106 64-65 0.6506449219424795 1106 66-67 0.5041128635133809 1106 68-69 0.5036872546977449 1106 70-71 0.4448614146453487 1106 72-73 0.4556991576766585 1106 74-75 0.2790019484595483 1106 76-77 0.3377411669727408 1106 78-79 0.2081705250287662 1106 80-81 0.005520630076869537 1106 82-83 -0.15398251007545127 1106 84-85 -0.18908505871793224 1106 86-87 0.009388077146912366 1106 88-89 -0.483757562209874 1106 90-91 -3.7291038807274024 1106 92-93 -8.689776444105572 1106 94-95 -6.228480397147258 1106 96-97 -3.3017824937879805 1106 98-99 -2.172086165105105 1106 100-101 -1.5715004058954118 1107 1 1.865465559583626 1107 2 1.1076472721083626 1107 3 0.833335735624825 1107 4 0.7669162702880499 1107 5 0.704852427675938 1107 6 0.6961192571831205 1107 7 0.8040313525741496 1107 8 0.658061729728189 1107 9 0.720783975108148 1107 10-11 0.6714853340474392 1107 12-13 0.6353757468416674 1107 14-15 0.7412753551363309 1107 16-17 0.6908931978836677 1107 18-19 0.8088412141351782 1107 20-21 0.7131762007860587 1107 22-23 0.6475943993450883 1107 24-25 0.7019229381222658 1107 26-27 0.6723295938349523 1107 28-29 0.5967662283457216 1107 30-31 0.7674900528867425 1107 32-33 0.8380619812085754 1107 34-35 0.7776160733651949 1107 36-37 0.4628309887621995 1107 38-39 0.5683637042658276 1107 40-41 0.5692297859418645 1107 42-43 0.44146875075424674 1107 44-45 0.36764277702212667 1107 46-47 0.4302439584349642 1107 48-49 0.49264639713804215 1107 50-51 0.4779651682900905 1107 52-53 0.5134830019847207 1107 54-55 0.4533134336938751 1107 56-57 0.27720338379085874 1107 58-59 0.284294647388986 1107 60-61 0.09135813138158255 1107 62-63 0.05856506285066487 1107 64-65 0.12788866123300124 1107 66-67 0.22479266274963905 1107 68-69 0.3253018462018247 1107 70-71 0.25161851743707686 1107 72-73 0.0704181886343136 1107 74-75 -0.030883408924410105 1107 76-77 0.08930190367325608 1107 78-79 0.08048869042303508 1107 80-81 -0.03354176396701902 1107 82-83 -0.18584115361454678 1107 84-85 0.032751287859642986 1107 86-87 0.29260726042833696 1107 88-89 0.07039672709598221 1107 90-91 -3.318077461408901 1107 92-93 -4.728310816141963 1107 94-95 -2.983109673472704 1107 96-97 -1.5761899745552057 1107 98-99 -1.0988084105498128 1107 100-101 -0.658872410911469 1108 1 1.6315824341386005 1108 2 0.8836348988541474 1108 3 -0.5179271872321642 1108 4 -0.19445007207114173 1108 5 0.3215754868791265 1108 6 0.5167042126340533 1108 7 0.3864356352064817 1108 8 0.5218959067202604 1108 9 0.6119457322099251 1108 10-11 0.5522753658161079 1108 12-13 0.586806399779654 1108 14-15 0.5023601257067227 1108 16-17 0.47365972911784837 1108 18-19 0.59774464345859 1108 20-21 0.39386970622015127 1108 22-23 0.32575526117793885 1108 24-25 0.407402218491697 1108 26-27 0.5307294004891148 1108 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2.9390970128905813 2311 92-93 3.775069712529625 2311 94-95 3.394192080774591 2311 96-97 2.7537486000646787 2311 98-99 2.472358797026745 2311 100-101 2.280007708008041 2312 1 1.2031354178760019 2312 2 0.5635762175557701 2312 3 -0.13603533343648522 2312 4 -0.6792474170748619 2312 5 -0.05040937938194645 2312 6 -0.23400603406315668 2312 7 0.02535107863420194 2312 8 0.20136912530525564 2312 9 0.24918232668004947 2312 10-11 0.18313303233336597 2312 12-13 0.191175321451027 2312 14-15 0.29199556672205773 2312 16-17 0.11460931220113935 2312 18-19 -0.3562112631360961 2312 20-21 0.3425203665935541 2312 22-23 0.4566006713455266 2312 24-25 0.5824919352900082 2312 26-27 0.5268312597120115 2312 28-29 0.5814941339260145 2312 30-31 0.5086510511493714 2312 32-33 0.7414560230444351 2312 34-35 0.6325115922372859 2312 36-37 0.5707636508209575 2312 38-39 0.47654318950220187 2312 40-41 0.5172458109843632 2312 42-43 0.5689003563370036 2312 44-45 0.41171447912897463 2312 46-47 0.6845541407704943 2312 48-49 0.7638224274099876 2312 50-51 0.6064985436736166 2312 52-53 0.48944946357504193 2312 54-55 0.5092047159860513 2312 56-57 0.5498740742779553 2312 58-59 0.5510573329329667 2312 60-61 0.5833742950356893 2312 62-63 0.576249489363498 2312 64-65 0.6019250436309385 2312 66-67 0.7019893724039896 2312 68-69 0.7819459715634274 2312 70-71 0.917727733236628 2312 72-73 0.773959557812514 2312 74-75 0.7751275746457367 2312 76-77 1.0605265530182528 2312 78-79 1.2204644718941253 2312 80-81 1.309496651576243 2312 82-83 1.2670568529159532 2312 84-85 1.5489644361161403 2312 86-87 1.9771817131531186 2312 88-89 2.3699927232505615 2312 90-91 3.1122918002759974 2312 92-93 3.96666615805405 2312 94-95 3.615184423615279 2312 96-97 2.8911743938947616 2312 98-99 2.7531202247716084 2312 100-101 2.626560611239965 2313 1 -7.296567353176414 2313 2 -2.6922246281719957 2313 3 -1.5948537196344077 2313 4 -0.8008703567316715 2313 5 -0.6534508018191403 2313 6 -0.29133185457038024 2313 7 -0.10164298350610323 2313 8 -0.04366413513976397 2313 9 -0.020525742336367614 2313 10-11 -0.19747239391109161 2313 12-13 -0.2970675855731173 2313 14-15 -0.3006953159859975 2313 16-17 -0.46417187646214586 2313 18-19 -0.5327758491077361 2313 20-21 -0.4644205507879562 2313 22-23 -1.172438709825279 2313 24-25 -0.4235435494080093 2313 26-27 -0.35058103063040136 2313 28-29 -0.633488133427214 2313 30-31 -0.13739505659469842 2313 32-33 -0.438298926761405 2313 34-35 -0.30828443596801947 2313 36-37 -0.12646930722159055 2313 38-39 -0.22816674357917321 2313 40-41 -0.23657978575371175 2313 42-43 -0.15178812226508143 2313 44-45 -0.2906683331344482 2313 46-47 -1.2863417683534095 2313 48-49 -0.7805753575752377 2313 50-51 -0.7564397560321758 2313 52-53 -1.0654439879299389 2313 54-55 -0.7157768972194312 2313 56-57 -0.6343945251145797 2313 58-59 -0.620405985332873 2313 60-61 -0.45124491519555576 2313 62-63 -0.2986992010167455 2313 64-65 -0.40238452752685205 2313 66-67 -0.32869530936909896 2313 68-69 -0.26842495672465816 2313 70-71 -0.14590612348621335 2313 72-73 -0.10772700501952315 2313 74-75 -0.12425501051903964 2313 76-77 0.027491398768798803 2313 78-79 -0.060873039387359995 2313 80-81 0.16691495067700046 2313 82-83 0.48966209590260945 2313 84-85 0.4616609316589404 2313 86-87 0.9873056363365826 2313 88-89 1.33341756304538 2313 90-91 2.0984685676296913 2313 92-93 3.0396113755756495 2313 94-95 2.7423172548688726 2313 96-97 1.970449131157718 2313 98-99 1.7077164654077084 2313 100-101 1.5612035829135564 2314 1 0.4624263886501794 2314 2 -0.10876761100383803 2314 3 -1.3102936014645081 2314 4 -0.3915310862568546 2314 5 -0.13403086013337884 2314 6 -3.5679658120863813 2314 7 -2.8404264737741016 2314 8 -2.4960606753986134 2314 9 -3.3777443169885473 2314 10-11 -2.610939555648361 2314 12-13 -1.2278661414978274 2314 14-15 -1.140113253218459 2314 16-17 -2.2671701529477346 2314 18-19 -3.3733718043792607 2314 20-21 -3.010123752494426 2314 22-23 -4.3146763349671 2314 24-25 -3.2670758383207925 2314 26-27 -2.0523025483265016 2314 28-29 -1.9866869400514204 2314 30-31 -1.9860592042245884 2314 32-33 -2.28572872573908 2314 34-35 -1.5760839202914596 2314 36-37 -2.0787988637455257 2314 38-39 -3.3617077612076223 2314 40-41 -2.7276076849405406 2314 42-43 -1.6543476835564377 2314 44-45 -2.233951321143472 2314 46-47 -1.8506603778722486 2314 48-49 -2.366942536818744 2314 50-51 -3.070191243042821 2314 52-53 -2.149317652261317 2314 54-55 -2.65554493531285 2314 56-57 -1.723108753724567 2314 58-59 -1.027097568203164 2314 60-61 -1.5409710118295479 2314 62-63 -1.1762222389133186 2314 64-65 -1.8658585289849015 2314 66-67 -1.1687212928687245 2314 68-69 -1.0757548133511925 2314 70-71 -1.0316612719569527 2314 72-73 -2.0518747110884625 2314 74-75 -2.201848034664458 2314 76-77 -1.2972139077624298 2314 78-79 -2.0770145274850442 2314 80-81 -1.0274374783374256 2314 82-83 -0.6456558207764687 2314 84-85 -1.6653367034968127 2314 86-87 -0.31984514071788794 2314 88-89 0.7431155729321333 2314 90-91 1.9044923158186968 2314 92-93 2.863359403892602 2314 94-95 2.5478005654294265 2314 96-97 1.8387697763155337 2314 98-99 1.8418640116798262 2314 100-101 1.7402984948784503 2315 1 -3.029706226082247 2315 2 -2.0964114314080042 2315 3 -2.9957858372709723 2315 4 -4.240482322562453 2315 5 -3.929336731481591 2315 6 -3.297040618802633 2315 7 -6.014775863575281 2315 8 -3.235373154463751 2315 9 -2.8076101341970947 2315 10-11 -4.012364250772009 2315 12-13 -3.1200521150335483 2315 14-15 -2.170613856508602 2315 16-17 -2.572310159734272 2315 18-19 -2.168065940107404 2315 20-21 -1.4354825768322783 2315 22-23 -1.0029609625090856 2315 24-25 -2.3647981499768775 2315 26-27 -4.040054729282588 2315 28-29 -3.2284650985576775 2315 30-31 -3.356394204778237 2315 32-33 -4.05969579846861 2315 34-35 -3.8686951151548357 2315 36-37 -3.002042134446029 2315 38-39 -1.846861027239882 2315 40-41 -1.6088104766061804 2315 42-43 -3.061371299480367 2315 44-45 -6.354652084710004 2315 46-47 -3.3727880979197806 2315 48-49 -2.3581231047258733 2315 50-51 -3.3201638375454827 2315 52-53 -3.3974890679476246 2315 54-55 -1.8454108054402347 2315 56-57 -1.4712443876613293 2315 58-59 -2.6553708944964463 2315 60-61 -2.883559050086049 2315 62-63 -4.5350621139842495 2315 64-65 -4.043452643523974 2315 66-67 -3.5518630676982674 2315 68-69 -1.8911905339917823 2315 70-71 -3.3982858466102535 2315 72-73 -2.3417486636454328 2315 74-75 -1.4014117588375647 2315 76-77 -3.191512204425006 2315 78-79 -1.7865749894578862 2315 80-81 -1.3718406635223168 2315 82-83 -0.9129226301455482 2315 84-85 -2.359817634762532 2315 86-87 -1.7689100356126168 2315 88-89 -1.1955911669557473 2315 90-91 -0.2751344205383859 2315 92-93 0.19720431163737473 2315 94-95 0.04708822248261413 2315 96-97 -0.09360630137891945 2315 98-99 -0.33772274326407903 2315 100-101 -0.7447786387898905 2316 1 -7.434972865972753 2316 2 -6.21298878536032 2316 3 -3.413545760457936 2316 4 -4.122778575875241 2316 5 -2.11150231980184 2316 6 -3.046058675152995 2316 7 -3.5188205810996678 2316 8 -2.861233944574245 2316 9 -7.311706762738744 2316 10-11 -7.0482757485947545 2316 12-13 -6.224461202095291 2316 14-15 -5.14365915621886 2316 16-17 -5.02563304215839 2316 18-19 -4.246251375230994 2316 20-21 -3.1728000425707705 2316 22-23 -3.761902468239672 2316 24-25 -3.4582816378361443 2316 26-27 -4.366496254813789 2316 28-29 -4.49168849981524 2316 30-31 -6.992180782173222 2316 32-33 -5.051443234956576 2316 34-35 -5.7340639873049675 2316 36-37 -4.451955111923613 2316 38-39 -6.733822401530627 2316 40-41 -4.1512954455516535 2316 42-43 -4.807039950121226 2316 44-45 -3.353334317811278 2316 46-47 -4.617613242242424 2316 48-49 -5.694798352932583 2316 50-51 -5.036792234150617 2316 52-53 -6.527853281942779 2316 54-55 -5.505738316246841 2316 56-57 -4.695236276183181 2316 58-59 -3.3322124545332095 2316 60-61 -3.380885394135287 2316 62-63 -4.500219754097955 2316 64-65 -3.218910832205445 2316 66-67 -4.1430785975661415 2316 68-69 -3.245450639296717 2316 70-71 -2.7999442413899125 2316 72-73 -2.3223438673537125 2316 74-75 -2.438637206572473 2316 76-77 -3.1840539558168466 2316 78-79 -5.13246143735206 2316 80-81 -5.984144614742757 2316 82-83 -3.6001520714026505 2316 84-85 -2.227465868151416 2316 86-87 -2.1935174418452377 2316 88-89 -1.2611843156938818 2316 90-91 -1.0578105212690474 2316 92-93 -1.2646131312469073 2316 94-95 -1.9344613232912131 2316 96-97 -3.307193800213547 2316 98-99 -4.510446156497437 2316 100-101 -3.9235672436727125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41585.0 3 14859.0 4 14816.0 5 16775.0 6 19045.0 7 21653.0 8 25477.0 9 30065.0 10 34954.0 11 40515.0 12 45753.0 13 50061.0 14 53746.0 15 59014.0 16 64478.0 17 69552.0 18 68440.0 19 71029.0 20 76557.0 21 84579.0 22 94324.0 23 105268.0 24 119402.0 25 133594.0 26 151337.0 27 170834.0 28 194165.0 29 218890.0 30 246856.0 31 278565.0 32 308962.0 33 345245.0 34 391729.0 35 446098.0 36 463269.0 37 341441.0 38 90013.0 39 1286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.40874132492998 18.688204946365747 11.809961487711726 24.093092240992554 2 16.787637396331885 19.679710510578044 37.86493548172174 25.667716611368334 3 20.07096404736379 23.597816774055165 28.41405178578646 27.917167392794585 4 13.225435987496246 14.804334170665726 35.4192201702587 36.55100967157933 5 14.308003856856132 37.22232326561025 33.50318626818356 14.966486609350055 6 34.49279271833518 34.193058812723145 16.762365524477303 14.551782944464378 7 30.612675894221752 31.390208675930253 19.91464394775745 18.082471482090543 8 28.237565223656848 33.57696841844016 19.399251841874037 18.786214516028956 9 27.808907964009755 15.040874258135068 18.96089566860374 38.18932210925144 10-11 26.279633125950447 25.464850077336987 26.645015980308216 21.61050081640435 12-13 26.571686064465954 22.610446171732416 27.168800581363584 23.64906718243805 14-15 23.516045344154186 24.174695643436976 24.61598413859277 27.69327487381607 16-17 23.668147589540094 27.36723254752057 25.43421741396783 23.530402448971504 18-19 23.704051181499942 26.122647881781248 27.130036203486956 23.043264733231855 20-21 24.162401664056652 25.485051255098835 27.142441912255883 23.21010516858863 22-23 24.00456522847018 25.362929956343176 27.110043973214577 23.522460841972066 24-25 23.67008198978708 25.59769823712444 27.327405339200574 23.40481443388791 26-27 23.3065321851658 25.88219680940302 27.216548436126715 23.594722569304462 28-29 23.417982681663954 25.9804386011124 27.257420005003723 23.344158712219926 30-31 23.472939330972377 25.870774513502422 27.05303796792692 23.603248187598286 32-33 23.244639764601 25.919837795652047 27.120156780444788 23.715365659302172 34-35 23.711598906800575 25.937371044234165 27.03457589155404 23.316454157411222 36-37 23.771830278422925 25.677600866521548 26.958706434579465 23.59186242047606 38-39 23.420161042901785 25.82934611166784 27.116081219965665 23.634411625464708 40-41 23.535038834349667 25.714264542616043 27.30477907384159 23.445917549192696 42-43 23.36228249827128 25.674510937141104 27.100481598275866 23.862724966311752 44-45 23.2427077812035 25.750577852784556 27.2978196082075 23.70889475780444 46-47 23.532554877641516 25.929566078827776 26.953893979113296 23.58398506441741 48-49 23.345386279476415 26.072240540674564 26.7963790071371 23.785994172711924 50-51 23.3450701473994 26.075181727275147 26.814322519556182 23.765425605769273 52-53 23.44197071023449 25.90249334175517 26.85027076277915 23.805265185231196 54-55 23.119561092676154 26.059401072544407 26.905743838760106 23.915293996019333 56-57 23.024544760133324 26.242082921002424 26.78406125408755 23.94931106477671 58-59 23.073623457335398 25.851526424976118 26.856330310548977 24.218519807139508 60-61 22.891577966164483 26.111909312726716 26.985721480256274 24.010791240852527 62-63 22.982134611926785 25.925256265320158 27.1272914907139 23.965317632039163 64-65 22.835664339514988 25.88336435790647 27.053631346776168 24.227339955802375 66-67 22.759686221753995 26.12515185135753 26.800121310874335 24.315040616014137 68-69 23.03356161640434 25.72776242540751 27.033010933557613 24.205665024630544 70-71 22.948090876612298 25.70655909206696 26.87327675840734 24.472073272913406 72-73 22.79225242287069 25.967700933650832 26.754424008931256 24.485622634547223 74-75 22.791837643945698 26.10199649643278 26.718711671282097 24.387454188339426 76-77 22.960325322100296 25.793729983939063 26.853980130603293 24.391964563357345 78-79 22.8987021521604 25.870413567220762 26.800019434408622 24.430864846210216 80-81 22.973792246632918 25.95607076569322 26.68238347030431 24.387753517369557 82-83 23.044797126674247 25.789787862988018 26.736525374244508 24.428889636093228 84-85 22.86924883442657 25.81942721879646 26.679175976195918 24.63214797058105 86-87 22.8801674163722 25.693398734861272 26.942009783784904 24.484424064981624 88-89 22.94336757360222 25.63474386412345 26.675414972725818 24.74647358954851 90-91 22.653940358955822 25.640639134771114 27.092555798443772 24.612864707829292 92-93 22.911589019770222 25.322407441559424 27.193279641891788 24.572723896778566 94-95 22.83954268719398 25.074412788652843 27.266391767791998 24.81965275636118 96-97 23.358777784513972 24.909364476606722 26.710380684730474 25.021477054148832 98-99 23.313191902262602 25.049037100596223 26.47231589034615 25.165455106795026 100-101 23.48319786393065 24.908764685877816 26.67809815075042 24.929939299441116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6324.0 1 3676.5 2 1008.5 3 987.0 4 875.5 5 780.0 6 981.5 7 1554.5 8 2270.0 9 2597.5 10 2416.5 11 2214.0 12 2246.0 13 2390.0 14 2597.5 15 2844.5 16 3124.5 17 3436.0 18 3812.0 19 4218.5 20 4772.5 21 5609.5 22 6791.5 23 8486.5 24 10223.5 25 12441.0 26 15805.0 27 19955.5 28 24119.5 29 29117.5 30 35435.5 31 43944.5 32 55906.5 33 64007.5 34 69816.5 35 79384.0 36 92395.5 37 105298.5 38 115473.5 39 123462.5 40 128883.5 41 133999.0 42 138510.5 43 147014.0 44 158176.0 45 167168.5 46 174376.5 47 182837.5 48 191627.0 49 196441.5 50 199627.0 51 203032.0 52 206042.5 53 208062.0 54 207288.5 55 199450.5 56 186290.5 57 171380.5 58 156799.5 59 140397.0 60 119091.5 61 97632.0 62 78758.5 63 61077.5 64 45775.5 65 34460.5 66 25886.0 67 19609.0 68 14975.0 69 11079.0 70 8116.5 71 5840.0 72 4163.0 73 3022.5 74 2263.5 75 1635.0 76 1132.0 77 799.5 78 547.0 79 360.0 80 244.5 81 193.5 82 146.0 83 89.0 84 56.0 85 41.0 86 45.0 87 38.5 88 24.5 89 21.0 90 13.0 91 10.5 92 9.0 93 7.0 94 5.0 95 4.5 96 4.5 97 5.5 98 3.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13718391497115143 2 0.12369532901258953 3 0.12967027301497475 4 0.14012142924657156 5 0.1274521499906779 6 0.14251940008364922 7 0.17357312242380496 8 0.15908538194979407 9 0.17073552359992974 10-11 0.1646306895105362 12-13 0.15390976155976813 14-15 0.15892551722732223 16-17 0.15356005747936097 18-19 0.16868725684325922 20-21 0.17441241221678216 22-23 0.2077342153070072 24-25 0.2305249298043995 26-27 0.24416338894027875 28-29 0.23879792919231746 30-31 0.22935591902132416 32-33 0.2301352595433744 34-35 0.21976403567301347 36-37 0.22770731407083328 38-39 0.21539773044050126 40-41 0.22052339310475477 42-43 0.21281991179064275 44-45 0.2060656272662073 46-47 0.22090307182062538 48-49 0.2609491847998224 50-51 0.2617385168670271 52-53 0.2645461410554389 54-55 0.2619583308604259 56-57 0.23541079538494528 58-59 0.23608022891029612 60-61 0.2071447141428923 62-63 0.19969102145764253 64-65 0.2012596940468975 66-67 0.20881330218369215 68-69 0.208443615012976 70-71 0.21451847446690608 72-73 0.2462316387872582 74-75 0.249019279885361 76-77 0.2645561326005934 78-79 0.2614487620575469 80-81 0.2761363334346476 82-83 0.2753070351868249 84-85 0.2494289332366951 86-87 0.2518768617995452 88-89 0.2555537504163976 90-91 0.4407870060354928 92-93 0.5834762623867683 94-95 0.3434094069598306 96-97 0.2918230593271973 98-99 0.3231465533865243 100-101 0.34225038772190974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5004231.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.02217341725531 #Duplication Level Percentage of deduplicated Percentage of total 1 71.27399571243312 29.950882079685766 2 12.561679026328864 10.557381089125807 3 5.351908766165256 6.746965148553759 4 2.9408228810203036 4.943190763826703 5 1.8795955072637698 3.9492344180266046 6 1.20854397289013 3.0471386646700647 7 0.8220297059543848 2.4180432400425387 8 0.6181064997244087 2.077934281740142 9 0.4688843423510473 1.7733185232220277 >10 2.5985825929131567 19.583468616324957 >50 0.1757466492910208 5.089498277823112 >100 0.09343746604252064 6.951231639344398 >500 0.0048530954019677264 1.4160684875650629 >1k 0.0017157399378248885 1.1890175074340654 >5k 9.804228216129938E-5 0.3066272626150767 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8613 0.1721143568312494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6272 0.12533394241792595 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 1.998309030898054E-5 0.0 0.0 0.0 8 0.0 3.996618061796108E-5 0.0 0.0 1.998309030898054E-5 9 0.0 3.996618061796108E-5 0.0 0.0 7.993236123592216E-5 10-11 0.0 3.996618061796108E-5 0.0 2.9974635463470813E-5 7.993236123592216E-5 12-13 0.0 3.996618061796108E-5 0.0 5.994927092694162E-5 7.993236123592216E-5 14-15 0.0 3.996618061796108E-5 0.0 1.0990699669939297E-4 7.993236123592216E-5 16-17 0.0 3.996618061796108E-5 0.0 1.998309030898054E-4 1.5986472247184433E-4 18-19 0.0 4.995772577245135E-5 0.0 3.0973789978919837E-4 1.5986472247184433E-4 20-21 0.0 5.994927092694162E-5 0.0 4.696026222610427E-4 1.5986472247184433E-4 22-23 0.0 5.994927092694162E-5 0.0 8.193067026682022E-4 1.5986472247184433E-4 24-25 0.0 5.994927092694162E-5 0.0 0.0014287909570921088 1.6985626762633458E-4 26-27 0.0 5.994927092694162E-5 0.0 0.002697717191712373 1.7984781278082486E-4 28-29 0.0 5.994927092694162E-5 0.0 0.009002382184195733 1.7984781278082486E-4 30-31 0.0 5.994927092694162E-5 0.0 0.0329621074646634 1.7984781278082486E-4 32-33 1.998309030898054E-5 5.994927092694162E-5 0.0 0.0878056988176605 1.7984781278082486E-4 34-35 1.998309030898054E-5 5.994927092694162E-5 0.0 0.1736530547850409 1.998309030898054E-4 36-37 1.998309030898054E-5 5.994927092694162E-5 0.0 0.3228168323964261 1.998309030898054E-4 38-39 1.998309030898054E-5 5.994927092694162E-5 0.0 0.5564890989244902 1.998309030898054E-4 40-41 1.998309030898054E-5 5.994927092694162E-5 0.0 0.8145307440843559 1.998309030898054E-4 42-43 1.998309030898054E-5 5.994927092694162E-5 0.0 1.0673667942187322 2.0982244824429566E-4 44-45 3.996618061796108E-5 5.994927092694162E-5 0.0 1.3621073847310408 2.1981399339878594E-4 46-47 3.996618061796108E-5 5.994927092694162E-5 0.0 1.7136299263563173 2.1981399339878594E-4 48-49 3.996618061796108E-5 5.994927092694162E-5 0.0 2.0922495384405715 2.1981399339878594E-4 50-51 5.994927092694162E-5 5.994927092694162E-5 0.0 2.504820420959784 2.1981399339878594E-4 52-53 5.994927092694162E-5 5.994927092694162E-5 0.0 2.8923524913218435 2.497886288622568E-4 54-55 5.994927092694162E-5 8.992390639041243E-5 0.0 3.2998576604477288 2.797632643257276E-4 56-57 5.994927092694162E-5 9.99154515449027E-5 0.0 3.7585694984903775 2.797632643257276E-4 58-59 5.994927092694162E-5 9.99154515449027E-5 0.0 4.26003515824909 2.797632643257276E-4 60-61 5.994927092694162E-5 1.1989854185388324E-4 0.0 4.796900862490161 2.797632643257276E-4 62-63 5.994927092694162E-5 1.1989854185388324E-4 0.0 5.304151626893323 2.997463546347081E-4 64-65 5.994927092694162E-5 1.1989854185388324E-4 0.0 5.815898586616005 2.997463546347081E-4 66-67 6.99408160814319E-5 1.1989854185388324E-4 0.0 6.382938757223638 2.997463546347081E-4 68-69 8.992390639041243E-5 1.1989854185388324E-4 0.0 7.000506171677527 2.997463546347081E-4 70-71 9.99154515449027E-5 1.298900870083735E-4 0.0 7.650885820418762 2.997463546347081E-4 72-73 9.99154515449027E-5 1.398816321628638E-4 0.0 8.293222275310633 2.997463546347081E-4 74-75 9.99154515449027E-5 1.398816321628638E-4 0.0 8.896251991564737 2.997463546347081E-4 76-77 1.1989854185388324E-4 1.5986472247184433E-4 0.0 9.540956442658223 2.997463546347081E-4 78-79 1.1989854185388324E-4 1.5986472247184433E-4 0.0 10.240844197639957 2.997463546347081E-4 80-81 1.1989854185388324E-4 1.5986472247184433E-4 0.0 10.962173808523227 2.997463546347081E-4 82-83 1.1989854185388324E-4 1.998309030898054E-4 0.0 11.644396511671824 3.1972944494368865E-4 84-85 1.1989854185388324E-4 1.998309030898054E-4 0.0 12.276321776512715 3.1972944494368865E-4 86-87 1.1989854185388324E-4 1.998309030898054E-4 0.0 12.917079567270175 3.1972944494368865E-4 88-89 1.398816321628638E-4 1.998309030898054E-4 0.0 13.598832667796511 3.397125352526692E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6055 0.0 48.417675 1 GTATCAA 11070 0.0 43.58158 1 ATCAACG 14555 0.0 33.799755 3 TCAACGC 14590 0.0 33.7522 4 TATCAAC 14875 0.0 33.07065 2 CAACGCA 15040 0.0 32.55372 5 AACGCAG 15620 0.0 31.314564 6 TACATGG 22090 0.0 31.18113 2 GTACATG 21685 0.0 30.845322 1 ACATGGG 23170 0.0 28.664806 3 ACGCAGA 18640 0.0 26.164713 7 CATGGGA 12140 0.0 25.635702 4 CGCAGAG 19025 0.0 25.510035 8 GAGTACA 15265 0.0 23.493872 1 CATGGGG 11705 0.0 22.73796 4 AGTACAT 15400 0.0 22.363348 2 GCAGAGT 21280 0.0 21.736454 9 ATGGGAT 5250 0.0 20.603657 5 ATGGGAG 5490 0.0 20.394281 5 GTGGTAT 3670 0.0 19.001194 1 >>END_MODULE