FastQCFastQC Report
Fri 27 May 2016
SRR939839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004231
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98580.19699330426593015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80240.16034431663925985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.10219352384012649No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.053.250661
GTATCAA116550.042.780911
TCAACGC149850.032.8536344
ATCAACG151650.032.5261963
CAACGCA152050.032.471325
TATCAAC157250.031.4881382
AACGCAG158750.031.1700466
GTACATG221300.028.9684561
TACATGG220750.028.9031682
ACATGGG224700.027.5133613
ACGCAGA195300.025.1918547
GAGTACA157700.024.5882421
CGCAGAG200800.024.4551438
CATGGGA118650.023.7271864
AGTACAT161300.022.6576922
CATGGGG110650.021.7775574
GCAGAGT225650.021.114569
ATGGGAG49950.019.542255
AGAGTAC201750.018.932410-11
ATGGGAT52500.018.6828755
Produced by FastQC (version 0.11.5)