##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939839_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5004231 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40842898739087 31.0 31.0 34.0 30.0 34.0 2 31.61314875352477 31.0 31.0 34.0 30.0 34.0 3 31.68007831772754 31.0 31.0 34.0 30.0 34.0 4 35.490037330411006 37.0 35.0 37.0 33.0 37.0 5 35.21519390291935 37.0 35.0 37.0 32.0 37.0 6 35.233903071221135 37.0 35.0 37.0 32.0 37.0 7 35.14359309152595 37.0 35.0 37.0 32.0 37.0 8 35.081672288909125 37.0 35.0 37.0 32.0 37.0 9 36.69183137229277 39.0 35.0 39.0 32.0 39.0 10-11 36.543397876716725 38.5 35.0 39.0 32.0 39.0 12-13 36.4863855205725 38.0 35.0 39.0 32.0 39.0 14-15 37.550666525985704 39.0 36.0 41.0 32.0 41.0 16-17 37.46873875326699 39.0 36.0 41.0 32.0 41.0 18-19 37.415527880307685 39.0 36.0 41.0 32.0 41.0 20-21 37.31993077457855 39.0 36.0 40.5 31.0 41.0 22-23 37.171104211616125 39.0 36.0 40.0 31.0 41.0 24-25 37.005069110518676 39.0 36.0 40.0 31.0 41.0 26-27 36.709570461475494 38.5 35.5 40.0 30.0 41.0 28-29 36.489452325442215 38.0 35.0 40.0 30.0 41.0 30-31 36.25006559449394 38.0 35.0 40.0 30.0 41.0 32-33 36.22870836697986 38.0 35.0 40.0 30.0 41.0 34-35 36.34430305075845 38.0 35.0 40.0 30.0 41.0 36-37 36.33506376903864 38.0 35.0 40.0 30.0 41.0 38-39 36.21294920238494 38.0 35.0 40.0 30.0 41.0 40-41 36.04468019162185 38.0 35.0 40.0 29.5 41.0 42-43 35.84599032298868 38.0 34.5 40.0 29.0 41.0 44-45 35.62464402622501 38.0 34.0 40.0 28.0 41.0 46-47 35.35246044796893 38.0 34.0 40.0 27.0 41.0 48-49 35.06449162718508 38.0 34.0 40.0 26.5 41.0 50-51 34.79357617584 38.0 33.0 40.0 26.0 41.0 52-53 34.489912436096574 37.5 33.0 40.0 26.0 41.0 54-55 34.159403013170255 37.0 33.0 40.0 24.5 41.0 56-57 32.84952383293257 36.5 31.5 39.5 18.0 41.0 58-59 29.509675512581254 34.5 24.5 38.5 8.0 40.0 60-61 29.466345878117934 34.0 25.0 38.0 9.5 40.0 62-63 31.27230857248596 35.0 29.5 38.0 13.5 40.0 64-65 30.80710962383631 35.0 29.0 38.0 7.5 40.0 66-67 25.38059124368959 29.0 18.0 35.5 2.0 39.0 68-69 17.64473532496801 13.0 7.0 29.0 2.0 35.5 70-71 16.48183307285375 15.0 7.5 26.0 2.0 33.5 72-73 18.688242409273272 19.0 7.5 30.0 2.0 34.0 74-75 19.416519840910624 20.0 8.5 30.5 2.0 35.0 76-77 20.37734339202167 23.5 8.5 31.5 2.0 34.5 78-79 23.536225645858472 28.5 12.0 34.0 2.0 35.5 80-81 26.234228795593168 31.5 23.0 34.5 2.0 36.0 82-83 27.18280431099204 33.0 26.0 35.0 2.0 36.0 84-85 27.176573683349147 33.0 26.0 35.0 2.0 35.5 86-87 26.899883018989332 33.0 25.5 35.0 2.0 35.0 88-89 26.48055695270662 33.0 25.0 35.0 2.0 35.0 90-91 24.92888857448827 31.5 15.0 34.5 2.0 35.0 92-93 22.882672182798917 31.0 2.0 34.0 2.0 35.0 94-95 18.41757504799439 24.0 2.0 33.0 2.0 35.0 96-97 17.089983555914984 15.5 2.0 32.5 2.0 34.5 98-99 15.984578249884947 6.0 2.0 32.0 2.0 34.0 100-101 15.338050141969866 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2934914117904981 1101 2 0.3503148465649524 1101 3 0.3120937895702838 1101 4 0.23891452409637992 1101 5 0.3285848210181612 1101 6 0.24985733121941678 1101 7 0.14982630614255754 1101 8 0.2924454639956551 1101 9 0.37999858289695965 1101 10-11 0.44519129007675673 1101 12-13 0.4611737126380362 1101 14-15 0.6256291120653898 1101 16-17 0.5162653244022763 1101 18-19 0.5124813186272945 1101 20-21 0.46291971841350943 1101 22-23 0.5916183514105029 1101 24-25 0.3853173790368274 1101 26-27 0.5910828222737408 1101 28-29 0.5412004594327442 1101 30-31 0.426356361154518 1101 32-33 0.5181825210419504 1101 34-35 0.7610739013802217 1101 36-37 0.5529751170165582 1101 38-39 0.4369882283611446 1101 40-41 0.47693519521763506 1101 42-43 0.6186733470112884 1101 44-45 0.251979547749265 1101 46-47 0.09107932847265232 1101 48-49 0.0968979388466309 1101 50-51 0.011630849370177998 1101 52-53 0.24922589586631716 1101 54-55 0.3082824123911294 1101 56-57 0.9746249256278858 1101 58-59 -8.045901413050874 1101 60-61 -9.884192974348817 1101 62-63 -2.9350777190379773 1101 64-65 -0.13947263915078878 1101 66-67 -1.3869612234091662 1101 68-69 -3.6017176017193915 1101 70-71 -4.73949486763658 1101 72-73 -6.8248053558452995 1101 74-75 -7.721912633805665 1101 76-77 -8.681875025792383 1101 78-79 -11.63130930793219 1101 80-81 -7.708266914090817 1101 82-83 -4.08124186267543 1101 84-85 -3.1594740422258276 1101 86-87 -3.206239143370855 1101 88-89 -3.487723207007477 1101 90-91 -9.02709225830839 1101 92-93 -14.165448521233497 1101 94-95 -12.42479116103574 1101 96-97 -12.319766894832988 1101 98-99 -11.880611479041495 1101 100-101 -11.784585203837885 1102 1 0.5083819905301539 1102 2 0.5095924168027288 1102 3 0.5018430538591119 1102 4 0.38908906062061277 1102 5 0.48403609926991464 1102 6 0.5615555471012783 1102 7 0.49675829406234584 1102 8 0.48700413380610996 1102 9 0.6430958749453453 1102 10-11 0.6740056295402752 1102 12-13 0.6648033076776656 1102 14-15 0.8024038574989234 1102 16-17 0.823185686452355 1102 18-19 0.8539208069405504 1102 20-21 0.8869931752017948 1102 22-23 0.8274173070124533 1102 24-25 0.8566400481464669 1102 26-27 0.8335045703709909 1102 28-29 0.8357273177159144 1102 30-31 0.7662417431452582 1102 32-33 0.6360023772256369 1102 34-35 0.6270601403890979 1102 36-37 0.7816797679583587 1102 38-39 0.6853557068094815 1102 40-41 0.7320980724644883 1102 42-43 0.8345488024692145 1102 44-45 0.8321502572391211 1102 46-47 0.7574437794435553 1102 48-49 0.7193717620799589 1102 50-51 0.6850392150965803 1102 52-53 0.7024975648244975 1102 54-55 0.6138647971940472 1102 56-57 1.2746774127919096 1102 58-59 -8.909306445434044 1102 60-61 -9.679219277322247 1102 62-63 -2.6541117770455713 1102 64-65 0.22580795559485267 1102 66-67 -0.8823169074646522 1102 68-69 -3.4904935334964726 1102 70-71 -4.0576377017652625 1102 72-73 -6.844056526598589 1102 74-75 -7.541477242757733 1102 76-77 -8.59537059296556 1102 78-79 -11.829821590077051 1102 80-81 -7.858260770791208 1102 82-83 -3.501715457251805 1102 84-85 -2.5292837892480904 1102 86-87 -2.2888889804898973 1102 88-89 -2.435892967719461 1102 90-91 -8.109543041384214 1102 92-93 -14.41893229178664 1102 94-95 -12.508534449805715 1102 96-97 -12.458360105206292 1102 98-99 -11.957815030499702 1102 100-101 -11.763362516465392 1103 1 0.27975081605076824 1103 2 0.23688050059391585 1103 3 0.2213271474000713 1103 4 0.17004797867836174 1103 5 0.2238502418901973 1103 6 0.22789874697363643 1103 7 0.38184652364994776 1103 8 0.28747204441364715 1103 9 0.3266143709515035 1103 10-11 0.29525579153861514 1103 12-13 0.3583517075942311 1103 14-15 0.4618186948149443 1103 16-17 0.43635686889506786 1103 18-19 0.44377123551556963 1103 20-21 0.47348943286235823 1103 22-23 0.4584661200300175 1103 24-25 0.422706087580103 1103 26-27 0.3529420703030084 1103 28-29 0.4998963931971403 1103 30-31 0.4948478596503634 1103 32-33 0.4429611607484105 1103 34-35 0.4238792920105965 1103 36-37 0.26334339657223893 1103 38-39 0.3867563931888114 1103 40-41 0.38572527071607254 1103 42-43 0.4923312738179888 1103 44-45 0.45024075091300375 1103 46-47 0.3898384022951049 1103 48-49 0.3915747838792214 1103 50-51 0.40411976648579895 1103 52-53 0.42751081124779944 1103 54-55 0.3393881762840536 1103 56-57 0.7643190988195911 1103 58-59 -6.677983191123548 1103 60-61 -8.604303882497678 1103 62-63 -2.3402314915804467 1103 64-65 -0.13541552363501452 1103 66-67 -1.9739633527504594 1103 68-69 -4.422517043147305 1103 70-71 -5.0315230883478925 1103 72-73 -7.750354741759658 1103 74-75 -8.192424032588594 1103 76-77 -9.081422755552406 1103 78-79 -12.21822866260035 1103 80-81 -8.529057764135494 1103 82-83 -4.751062778596186 1103 84-85 -3.8928764811212666 1103 86-87 -3.676107179724305 1103 88-89 -3.8237343533359187 1103 90-91 -9.457255712678894 1103 92-93 -15.250302143889371 1103 94-95 -12.969779113272937 1103 96-97 -12.778487941871472 1103 98-99 -12.302121427863286 1103 100-101 -11.956037986917355 1104 1 0.13290860097913182 1104 2 0.13090898047673605 1104 3 0.17874332542633553 1104 4 0.1847400013302405 1104 5 0.07867235580638265 1104 6 0.1212052122004792 1104 7 0.13663063832466804 1104 8 0.19936728946515103 1104 9 0.2780939032839598 1104 10-11 0.23593059309994402 1104 12-13 0.17424322074088394 1104 14-15 0.22894313401001654 1104 16-17 0.29461853549427985 1104 18-19 0.3305178536811866 1104 20-21 0.256478028511701 1104 22-23 0.22446150067682424 1104 24-25 0.25145025007471133 1104 26-27 0.23105683266313548 1104 28-29 0.1463966405301278 1104 30-31 0.29296349986636727 1104 32-33 0.2088120912284026 1104 34-35 0.2030501048143023 1104 36-37 0.18877800608370165 1104 38-39 0.1302805165301848 1104 40-41 0.10656414121496027 1104 42-43 0.11085429802275826 1104 44-45 0.09873265192953085 1104 46-47 0.07441370427062566 1104 48-49 0.009596093103880321 1104 50-51 -0.01911618831072559 1104 52-53 -0.1639877141650743 1104 54-55 -0.13318647616532076 1104 56-57 0.5686069814799168 1104 58-59 -6.601969701792516 1104 60-61 -8.794144502967374 1104 62-63 -2.73786877301486 1104 64-65 -0.47951095456232906 1104 66-67 -1.9674883117989914 1104 68-69 -4.445623165385394 1104 70-71 -4.919299624757365 1104 72-73 -8.015679394735892 1104 74-75 -8.865173551312106 1104 76-77 -9.249281407094566 1104 78-79 -12.429497450659998 1104 80-81 -8.738544842685645 1104 82-83 -4.982136845432848 1104 84-85 -4.142016574782964 1104 86-87 -3.8748817641398468 1104 88-89 -3.9675870622007565 1104 90-91 -9.515457466341344 1104 92-93 -15.252230719849448 1104 94-95 -12.877309986657414 1104 96-97 -12.534988017702812 1104 98-99 -11.92364353257216 1104 100-101 -11.49210705368171 1105 1 0.17038694485792405 1105 2 0.13940067959611824 1105 3 0.11787958551079214 1105 4 0.17039127185502423 1105 5 0.1347918657511471 1105 6 0.15854529779354465 1105 7 0.18476902322667854 1105 8 0.2184868048504569 1105 9 0.17949246155516363 1105 10-11 0.19696209198222192 1105 12-13 0.2149791375612935 1105 14-15 0.2344990892574046 1105 16-17 0.25663524831107765 1105 18-19 0.24187291081279483 1105 20-21 0.24697132562902624 1105 22-23 0.24013614385231818 1105 24-25 0.2595923467209076 1105 26-27 0.29678864149278894 1105 28-29 0.31293412916538443 1105 30-31 0.29153754484459427 1105 32-33 0.23223079632656152 1105 34-35 0.2751904933581244 1105 36-37 0.2048974992883359 1105 38-39 0.2652322073578688 1105 40-41 0.3611358051374438 1105 42-43 0.16971131441579956 1105 44-45 0.18346814583328097 1105 46-47 0.16533277527386048 1105 48-49 0.19202698802495632 1105 50-51 0.11681771083659243 1105 52-53 0.0479337606072221 1105 54-55 0.04798508513254518 1105 56-57 0.7127645664021358 1105 58-59 -6.631259133719503 1105 60-61 -8.79749028499965 1105 62-63 -2.658780030560713 1105 64-65 -0.3766953934362789 1105 66-67 -1.52903927224704 1105 68-69 -3.93912603416873 1105 70-71 -4.762342381455616 1105 72-73 -5.9318252118006995 1105 74-75 -6.544965478298948 1105 76-77 -8.44346853645041 1105 78-79 -11.95257197480553 1105 80-81 -8.009093651608012 1105 82-83 -3.86778809313018 1105 84-85 -2.9916237846672225 1105 86-87 -2.813552230005211 1105 88-89 -2.924261427680257 1105 90-91 -8.515955979912588 1105 92-93 -11.768048205824545 1105 94-95 -11.884872950770486 1105 96-97 -12.345885703946216 1105 98-99 -11.734975910627231 1105 100-101 -11.291100374330334 1106 1 0.11495530601661841 1106 2 0.17154824164118665 1106 3 0.12676590712139202 1106 4 0.09932361689043034 1106 5 0.09880706090314817 1106 6 0.05206884442733184 1106 7 0.16959601433823934 1106 8 0.18780109619227403 1106 9 0.20664179647671688 1106 10-11 0.23576812543056036 1106 12-13 0.22245521188467166 1106 14-15 0.2531626156524638 1106 16-17 0.14327934096228034 1106 18-19 0.11962670587968915 1106 20-21 0.1782098985276903 1106 22-23 0.2282699624855269 1106 24-25 0.2725367713761031 1106 26-27 0.3059915729476188 1106 28-29 0.371732439029941 1106 30-31 0.20443285093238472 1106 32-33 0.2352450737422771 1106 34-35 0.22069404431043438 1106 36-37 0.15190918803931908 1106 38-39 0.2446143981356883 1106 40-41 0.34706542550944164 1106 42-43 0.31877468715477164 1106 44-45 0.27254710438916163 1106 46-47 0.21430600555267176 1106 48-49 0.250394648963713 1106 50-51 0.30981286076035985 1106 52-53 0.2731680015296192 1106 54-55 0.05657163871571669 1106 56-57 0.9816141989508012 1106 58-59 -6.542432435402727 1106 60-61 -8.990720731008064 1106 62-63 -2.3903878388837114 1106 64-65 0.11816424347779986 1106 66-67 -1.2994004884382626 1106 68-69 -3.893715704507498 1106 70-71 -4.650976887320585 1106 72-73 -6.187789162156896 1106 74-75 -6.728829508969813 1106 76-77 -8.416451366972595 1106 78-79 -11.93263269098238 1106 80-81 -8.041934908921 1106 82-83 -4.00498966826823 1106 84-85 -2.9744055145175494 1106 86-87 -2.884131268386408 1106 88-89 -2.962517675719031 1106 90-91 -8.197663789071562 1106 92-93 -11.784078088019502 1106 94-95 -11.931272869701402 1106 96-97 -12.383431312952224 1106 98-99 -11.834112940827708 1106 100-101 -11.609811469682542 1107 1 0.0460173114071587 1107 2 0.06713328132127927 1107 3 0.03226079744321453 1107 4 -0.012926959257796966 1107 5 -0.0778115942550599 1107 6 0.01480508544099024 1107 7 0.08685657503141897 1107 8 0.0974665203153009 1107 9 0.019442256383172207 1107 10-11 0.05788553720169176 1107 12-13 0.11382842334549537 1107 14-15 0.061052076163157665 1107 16-17 0.08420372865614212 1107 18-19 0.11381279593592808 1107 20-21 0.09566195196347138 1107 22-23 0.1332591986096361 1107 24-25 0.1693892865401594 1107 26-27 0.24334895285727498 1107 28-29 0.25948854286111356 1107 30-31 0.20384997212441647 1107 32-33 0.22673720907866368 1107 34-35 0.05509431879686133 1107 36-37 0.06450783159866091 1107 38-39 0.17085558385227273 1107 40-41 0.016338937265693687 1107 42-43 0.14667301381993525 1107 44-45 0.21332119254666537 1107 46-47 0.05981103729776294 1107 48-49 0.03190072116618836 1107 50-51 0.1872782539125737 1107 52-53 0.02932071800396585 1107 54-55 0.0018021840348225737 1107 56-57 0.6551717029461415 1107 58-59 -6.753662796516341 1107 60-61 -9.094730236821167 1107 62-63 -2.6206055353636657 1107 64-65 -0.3360380202255193 1107 66-67 -1.722487408127705 1107 68-69 -4.026879474323506 1107 70-71 -4.650427408409584 1107 72-73 -6.1816362938648375 1107 74-75 -6.752644040354076 1107 76-77 -9.063985999123336 1107 78-79 -12.457226958143096 1107 80-81 -8.49953910394806 1107 82-83 -4.3325830437529085 1107 84-85 -3.3599489257806 1107 86-87 -3.162060717682838 1107 88-89 -3.234965189805184 1107 90-91 -8.567144177765392 1107 92-93 -11.869466139155758 1107 94-95 -12.070022316831896 1107 96-97 -12.681037350034206 1107 98-99 -11.933038881110717 1107 100-101 -11.4820293341741 1108 1 0.010982502926179194 1108 2 0.017547805859450705 1108 3 -0.008851175670134381 1108 4 -0.020195165044320618 1108 5 -0.1417265763221991 1108 6 0.03273285486544353 1108 7 -0.03626635605633055 1108 8 -0.06543218676684859 1108 9 -0.026219353950203583 1108 10-11 0.011967732637110373 1108 12-13 -0.026874082418054 1108 14-15 0.03648212956780128 1108 16-17 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2312 28-29 -0.5093109983204513 2312 30-31 -0.35358369688120206 2312 32-33 -0.2922631176737127 2312 34-35 -0.37447465334377483 2312 36-37 -0.35340059617987407 2312 38-39 -0.4546274259868781 2312 40-41 -0.3427276185350152 2312 42-43 -0.28674633338587796 2312 44-45 -0.22998385783392195 2312 46-47 -0.2655704290345824 2312 48-49 -0.34052706647172215 2312 50-51 -0.29298462511011536 2312 52-53 -0.2754859721765115 2312 54-55 -0.3391124635897782 2312 56-57 0.9556321490200119 2312 58-59 4.445445888813776 2312 60-61 4.050579729021219 2312 62-63 1.6157429797331844 2312 64-65 1.4108655241189112 2312 66-67 -0.0625222045427769 2312 68-69 -0.9388526823790109 2312 70-71 0.003321851934703801 2312 72-73 6.954155289575066 2312 74-75 5.552336194365086 2312 76-77 5.83165501570268 2312 78-79 5.7979060206222 2312 80-81 3.2060336604102737 2312 82-83 1.840871379891432 2312 84-85 1.494664976999104 2312 86-87 1.3764563865875736 2312 88-89 1.4618348771204204 2312 90-91 3.0179463056898577 2312 92-93 4.939689017817518 2312 94-95 8.92124378648591 2312 96-97 9.553169108111824 2312 98-99 10.048972324378022 2312 100-101 9.231410549252136 2313 1 -0.31919155989679027 2313 2 -0.2798370719765728 2313 3 -0.3022921967145322 2313 4 -0.43019797451388797 2313 5 -0.42546152037602525 2313 6 -0.3882651509979098 2313 7 -0.3438518860451296 2313 8 -0.3764637491669518 2313 9 -0.3424090027993145 2313 10-11 -0.4440889582064784 2313 12-13 -0.39136391069340704 2313 14-15 -0.594336313145682 2313 16-17 -0.47347223482400835 2313 18-19 -0.5151815433242888 2313 20-21 -0.448770274087849 2313 22-23 -0.45646242698204276 2313 24-25 -0.5051413334669874 2313 26-27 -0.6044476921286233 2313 28-29 -0.6765030270827097 2313 30-31 -0.6976227762883269 2313 32-33 -0.6278999540079013 2313 34-35 -0.6879471350414335 2313 36-37 -0.7308091502569383 2313 38-39 -1.0097855798391109 2313 40-41 -1.1697791649255223 2313 42-43 -0.8174012154297259 2313 44-45 -0.8864042486707788 2313 46-47 -0.766955724739347 2313 48-49 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72-73 8.137610901451943 2314 74-75 9.74825882332917 2314 76-77 8.740948829487948 2314 78-79 6.333155960386751 2314 80-81 3.2458009658909575 2314 82-83 1.7125706492751647 2314 84-85 1.3368647964476033 2314 86-87 1.2594806425688638 2314 88-89 1.346891668033539 2314 90-91 2.666612978590802 2314 92-93 4.695833447351479 2314 94-95 8.738066191325697 2314 96-97 9.441727809134495 2314 98-99 9.757376238105282 2314 100-101 8.97556313995689 2315 1 -0.7259047393371141 2315 2 -0.5227476630651751 2315 3 -0.5210931046487062 2315 4 -0.36056838379922596 2315 5 -0.6487191034278155 2315 6 -0.5851676948889306 2315 7 -0.6199334970237018 2315 8 -0.7585735871522559 2315 9 -0.962081353904928 2315 10-11 -0.769826576106631 2315 12-13 -0.6624935079903835 2315 14-15 -0.7911657270411325 2315 16-17 -0.9973192727709304 2315 18-19 -0.8347480625008359 2315 20-21 -0.8611281827685247 2315 22-23 -0.885365180150643 2315 24-25 -0.849613302372795 2315 26-27 -0.8483478658616974 2315 28-29 -0.9047249868549798 2315 30-31 -0.8972873803006536 2315 32-33 -0.7636596555018684 2315 34-35 -0.8336144248237574 2315 36-37 -0.8376013884019997 2315 38-39 -0.8202921496535467 2315 40-41 -0.6621520794373268 2315 42-43 -0.6636197641748254 2315 44-45 -0.5775915385049046 2315 46-47 -0.6633032976728757 2315 48-49 -0.7121547804574107 2315 50-51 -0.6019613449053054 2315 52-53 -0.4218855342109933 2315 54-55 -0.5876087017201073 2315 56-57 0.7911361039155196 2315 58-59 4.178949197652205 2315 60-61 3.7283124674387302 2315 62-63 1.1322072325239958 2315 64-65 1.0878714872649944 2315 66-67 1.9403362350926194 2315 68-69 6.016665658486101 2315 70-71 5.028589216541146 2315 72-73 9.095119504685968 2315 74-75 10.213614259630685 2315 76-77 9.043804234638117 2315 78-79 6.3648727278099635 2315 80-81 3.412886460551185 2315 82-83 1.8449227104316215 2315 84-85 1.2109702950133645 2315 86-87 1.2042458972163175 2315 88-89 1.2220538050476115 2315 90-91 2.608600624934084 2315 92-93 4.618605986523189 2315 94-95 8.65731112072412 2315 96-97 9.409482468661299 2315 98-99 9.740592797714369 2315 100-101 8.93403242009424 2316 1 -0.4972519762348533 2316 2 -0.5014527904518538 2316 3 -0.577617045599542 2316 4 -0.4147121473537041 2316 5 -0.6469744542942877 2316 6 -0.5896674449240251 2316 7 -0.5487965092883513 2316 8 -0.7055485823595475 2316 9 -0.6879411802263178 2316 10-11 -0.5703487112411167 2316 12-13 -0.6181112581611714 2316 14-15 -0.7028415644951451 2316 16-17 -0.9506079931652991 2316 18-19 -0.9518049731025187 2316 20-21 -0.7606700584987038 2316 22-23 -0.8310426862003197 2316 24-25 -2.3532495546225576 2316 26-27 -1.6335887907137234 2316 28-29 -1.149727096217184 2316 30-31 -0.8319821277740687 2316 32-33 -0.8084089563015056 2316 34-35 -0.7261414426474602 2316 36-37 -0.7110387975994641 2316 38-39 -0.9960085574760384 2316 40-41 -0.855570573557884 2316 42-43 -0.952018482010871 2316 44-45 -0.8909880830442205 2316 46-47 -0.7964284811864317 2316 48-49 -1.0348389598401297 2316 50-51 -1.0983612586032407 2316 52-53 -0.8420335550414322 2316 54-55 -0.8219361787463768 2316 56-57 0.2641201409914373 2316 58-59 3.8711904376918973 2316 60-61 3.8454114603172833 2316 62-63 1.241466946110691 2316 64-65 0.8319449237962857 2316 66-67 6.034508307327943 2316 68-69 8.9620686277772 2316 70-71 5.624641393220678 2316 72-73 9.468818759161696 2316 74-75 10.372674387761197 2316 76-77 8.949450569267029 2316 78-79 6.314149222778074 2316 80-81 3.271959929049739 2316 82-83 1.8977072927831529 2316 84-85 1.6315478699692 2316 86-87 1.443662991838746 2316 88-89 1.4338166411678692 2316 90-91 2.9557912417123546 2316 92-93 4.844778090057805 2316 94-95 8.969028668298805 2316 96-97 9.583784730674335 2316 98-99 9.881398998714397 2316 100-101 8.755584433549881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 36.0 4 88.0 5 243.0 6 495.0 7 1339.0 8 2970.0 9 4989.0 10 7637.0 11 11778.0 12 16581.0 13 22881.0 14 29573.0 15 40394.0 16 53847.0 17 69920.0 18 77971.0 19 83282.0 20 86910.0 21 87130.0 22 82995.0 23 80226.0 24 84282.0 25 94004.0 26 124682.0 27 180566.0 28 250030.0 29 324181.0 30 383327.0 31 414079.0 32 430890.0 33 446745.0 34 451446.0 35 431098.0 36 354431.0 37 211061.0 38 59901.0 39 2217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80128254840337 18.65433553647252 12.68705163372618 24.85733028139793 2 16.89321866777831 20.500651278716514 37.042750982113915 25.563379071391257 3 19.877279035818095 23.82461353687574 28.96456559556438 27.333541831741787 4 13.600071619395667 15.457060235628612 35.19289976821614 35.74996837675958 5 14.076708058947258 37.55917051205426 33.66132021652413 14.702801212474354 6 34.5632471435143 34.734882077667955 16.289771884802963 14.412098894014779 7 30.37511657635309 31.570944666623102 19.87408255134505 18.179856205678753 8 27.995158492042528 34.1975762085885 19.143000336115914 18.664264963253057 9 27.346319544401528 15.061355081330177 18.943949629823244 38.648375744445055 10-11 26.183583451683184 25.684635661303407 26.62198247842675 21.509798408586654 12-13 26.32036770484816 22.69737348255906 27.31749393663082 23.66476487596196 14-15 23.17343003833263 24.326466299210317 24.679727969729566 27.820375692727488 16-17 23.399469369019936 27.568191796102138 25.54145681923956 23.490882015638366 18-19 23.41122463681666 26.263325681688787 27.257280103146325 23.068169578348225 20-21 23.8930417081066 25.585719364273952 27.291256139055132 23.229982788564314 22-23 23.7252636818724 25.46976748275609 27.25363797154848 23.551330863823033 24-25 23.505389739202688 25.767995122527314 27.358689077302788 23.36792606096721 26-27 23.157547345794885 26.0317817793564 27.253340905609758 23.55732996923896 28-29 23.33326472325626 26.043978926181687 27.26312337583961 23.359632974722448 30-31 23.32984764041239 25.91952595908599 27.083144518027595 23.667481882474025 32-33 23.131949019755954 25.941411766445594 27.202179443600823 23.72445977019763 34-35 23.551072499355787 26.019281298187884 27.07763746495302 23.35200873750331 36-37 23.63640034175628 25.745772944747635 27.0307169896025 23.587109723893587 38-39 23.322726459586114 25.88504600698175 27.184654545381864 23.607572988050276 40-41 23.40213146414133 25.713959335159725 27.408490917151614 23.475418283547334 42-43 23.245061155827806 25.72893231378137 27.15639967207128 23.869606858319543 44-45 23.093769535632244 25.820720175538973 27.371637622548224 23.713872666280555 46-47 23.38490365320471 25.94134539907848 27.02753430919764 23.646216638519167 48-49 23.188310343614475 26.101949382107996 26.8579136230475 23.851826651230027 50-51 23.26249546651044 26.051627164219703 26.871957664977796 23.813919704292054 52-53 23.398992083453923 25.83061973236286 26.898418049916934 23.871970134266284 54-55 23.045965978877977 26.00105667079149 26.958865686789547 23.99411166354099 56-57 22.874010929622333 26.077813891402073 27.013563607473866 24.034611571501724 58-59 21.17049864128465 25.682438342351166 28.882154598772242 24.26490841759194 60-61 22.697889500059084 25.985159057986955 27.30574371136205 24.01120773059191 62-63 22.79183243953618 25.938791755704173 27.14128889236596 24.12808691239369 64-65 22.04899806252199 25.82524916863743 27.582937146228158 24.54281562261242 66-67 21.622477242537776 25.921587535919233 28.177836337598038 24.27809888394495 68-69 22.001249657935663 25.142347539421472 28.856172791650604 24.000230010992265 70-71 21.207005859052423 25.36463186575627 28.96984431117908 24.458517964012223 72-73 21.01836009978514 25.601962726117062 28.711434274018306 24.66824290007949 74-75 21.61746909242209 25.656610657120606 28.044002547195422 24.68191770326188 76-77 20.378629505274827 25.822273595762763 28.725716150635392 25.07338074832702 78-79 21.433590863905305 25.90614280846781 27.428952343801104 25.231313983825782 80-81 22.246165359519246 25.9325159228191 26.56840954654749 25.252909171114162 82-83 22.420887802339703 25.736518084129763 26.432800143558776 25.409793969971755 84-85 22.172426561614188 25.6753338026697 26.427117943643267 25.725121692072843 86-87 22.305220722220838 25.66736938952039 26.442332911979392 25.58507697627938 88-89 22.253413612084177 25.627099473845128 26.237073435873253 25.882413478197446 90-91 21.769229359593304 25.622196068354725 26.60142730954444 26.007147262507534 92-93 21.527842208452324 25.420535885058275 26.804301597009978 26.24732030947942 94-95 19.934426037049207 25.28674186810294 28.230495839840515 26.54833625500734 96-97 19.877089133143492 25.086018888895072 28.352181925890253 26.684710052071186 98-99 20.23528552294159 25.210169336234816 27.733725031890927 26.82082010893267 100-101 20.253437949383635 25.004255899700205 27.766703465862268 26.97560268505389 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 423.0 1 312.0 2 232.0 3 320.5 4 382.0 5 441.5 6 609.5 7 902.5 8 1282.0 9 1549.5 10 1677.5 11 1891.0 12 2180.0 13 2433.0 14 2626.5 15 2908.0 16 3234.0 17 3498.0 18 3859.5 19 4334.5 20 4943.0 21 5866.0 22 7041.0 23 8481.5 24 10436.5 25 12958.5 26 16047.0 27 20091.5 28 24767.5 29 30142.5 30 36831.0 31 45266.0 32 55936.5 33 66097.5 34 74928.0 35 85048.0 36 96931.0 37 108074.0 38 117105.0 39 125038.0 40 132727.5 41 140178.5 42 147885.0 43 157471.0 44 167168.5 45 175816.0 46 184168.0 47 192647.0 48 200029.0 49 205702.0 50 209999.5 51 213252.0 52 214867.5 53 213864.0 54 209544.0 55 199499.5 56 184632.5 57 166809.0 58 147288.5 59 126515.0 60 104469.0 61 83331.5 62 64550.5 63 48251.5 64 35471.5 65 26146.0 66 19139.0 67 13850.5 68 9888.5 69 6952.5 70 4807.5 71 3240.5 72 2279.0 73 1720.5 74 1179.0 75 740.5 76 477.5 77 297.0 78 169.5 79 106.5 80 71.0 81 42.5 82 28.0 83 19.0 84 8.0 85 5.0 86 5.5 87 3.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021321957359682238 2 0.004576127680756544 3 0.003916685700560186 4 0.0 5 0.06430558461429937 6 9.99154515449027E-5 7 0.0 8 9.99154515449027E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 1.698562676263346E-4 16-17 0.0 18-19 0.0015586810441004823 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.298055385532762E-4 28-29 0.0021381906630609177 30-31 0.00886250055203287 32-33 3.596956255616497E-4 34-35 0.00110906151214842 36-37 0.024988854431380162 38-39 0.02369994510645092 40-41 0.025678271047039996 42-43 0.02626777221115492 44-45 0.027047112733205165 46-47 0.028336022058134406 48-49 0.028276072787207465 50-51 0.02370993665160541 52-53 0.02076243083103078 54-55 0.015596801986159313 56-57 0.15605794376798354 58-59 0.7548612364217399 60-61 0.31036736713393126 62-63 3.896702610251206E-4 64-65 0.14014141233688054 66-67 1.403712178754338 68-69 2.6956889080460114 70-71 2.1778670888693985 72-73 1.8319697871661 74-75 1.5435638362817383 76-77 1.1135776905582495 78-79 0.4145492084598013 80-81 2.697717191712373E-4 82-83 1.7984781278082486E-4 84-85 3.996618061796108E-5 86-87 0.002487894743468077 88-89 1.998309030898054E-5 90-91 0.22242178668410792 92-93 0.1708853967772471 94-95 1.2760302232251068 96-97 1.2477141842572814 98-99 0.9803704105585852 100-101 0.39913225428642285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5004231.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.05074953316357 #Duplication Level Percentage of deduplicated Percentage of total 1 64.10998470704082 21.829930318343933 2 14.646334364546778 9.974373260522974 3 6.8154830233031145 6.962169161220682 4 3.8260427373687262 5.21118491813288 5 2.3566416210839347 4.012270678947881 6 1.6062109721629727 3.2815612506324325 7 1.161807844929248 2.769229955334525 8 0.8463263442388075 2.305443709679488 9 0.6420030936271309 1.9674617886552237 >10 3.5560590959726484 21.95165325689684 >50 0.2695616043876084 6.315726720685072 >100 0.15245948992236674 9.396217965918403 >500 0.007783157275411098 1.8149209957883143 >1k 0.0031250542758017976 1.7461551724558453 >5k 1.768898646679557E-4 0.4617008467855313 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9858 0.19699330426593015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8024 0.16034431663925985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5114 0.10219352384012649 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.998309030898054E-5 2 0.0 0.0 0.0 0.0 1.998309030898054E-5 3 0.0 0.0 0.0 0.0 1.998309030898054E-5 4 0.0 0.0 0.0 0.0 1.998309030898054E-5 5 0.0 0.0 0.0 0.0 1.998309030898054E-5 6 0.0 0.0 0.0 0.0 1.998309030898054E-5 7 0.0 0.0 0.0 0.0 1.998309030898054E-5 8 0.0 0.0 0.0 0.0 1.998309030898054E-5 9 0.0 0.0 0.0 0.0 1.998309030898054E-5 10-11 0.0 9.99154515449027E-6 0.0 9.99154515449027E-6 1.998309030898054E-5 12-13 0.0 1.998309030898054E-5 0.0 3.996618061796108E-5 1.998309030898054E-5 14-15 0.0 1.998309030898054E-5 0.0 8.992390639041243E-5 2.9974635463470813E-5 16-17 0.0 1.998309030898054E-5 0.0 1.998309030898054E-4 3.996618061796108E-5 18-19 0.0 1.998309030898054E-5 0.0 3.2972099009817893E-4 6.99408160814319E-5 20-21 0.0 1.998309030898054E-5 0.0 4.6960262226104267E-4 7.993236123592216E-5 22-23 0.0 1.998309030898054E-5 0.0 8.093151575137118E-4 1.1989854185388325E-4 24-25 0.0 1.998309030898054E-5 0.0 0.0014088078667831281 1.7984781278082486E-4 26-27 0.0 1.998309030898054E-5 0.0 0.0027376833723303344 1.998309030898054E-4 28-29 0.0 1.998309030898054E-5 0.0 0.009372069354911874 1.998309030898054E-4 30-31 0.0 1.998309030898054E-5 0.0 0.03508031503741534 1.998309030898054E-4 32-33 0.0 1.998309030898054E-5 0.0 0.09312120083984932 1.998309030898054E-4 34-35 0.0 1.998309030898054E-5 0.0 0.1829951495044893 2.1981399339878594E-4 36-37 0.0 1.998309030898054E-5 0.0 0.3399823069718404 2.1981399339878594E-4 38-39 0.0 1.998309030898054E-5 0.0 0.5855545037789023 2.1981399339878594E-4 40-41 0.0 1.998309030898054E-5 0.0 0.8563653436462066 2.1981399339878594E-4 42-43 0.0 1.998309030898054E-5 0.0 1.1216308759527687 2.1981399339878594E-4 44-45 0.0 1.998309030898054E-5 0.0 1.4303995958619815 2.1981399339878594E-4 46-47 0.0 1.998309030898054E-5 0.0 1.786318417355234 2.2980553855327622E-4 48-49 0.0 1.998309030898054E-5 0.0 2.1408284309817036 2.3979708370776648E-4 50-51 0.0 1.998309030898054E-5 0.0 2.507288332612943 2.3979708370776648E-4 52-53 0.0 1.998309030898054E-5 0.0 2.844782744841315 2.4978862886225673E-4 54-55 0.0 1.998309030898054E-5 0.0 3.205827228998821 2.59780174016747E-4 56-57 0.0 1.998309030898054E-5 0.0 3.516933970474185 2.59780174016747E-4 58-59 0.0 1.998309030898054E-5 0.0 3.7347296717517637 2.59780174016747E-4 60-61 0.0 3.996618061796108E-5 9.99154515449027E-6 3.900199651055277 2.697717191712373E-4 62-63 3.996618061796108E-5 3.996618061796108E-5 1.998309030898054E-5 4.04039102111793 2.797632643257276E-4 64-65 5.994927092694162E-5 5.994927092694162E-5 1.998309030898054E-5 4.185478248306284 2.797632643257276E-4 66-67 5.994927092694162E-5 6.99408160814319E-5 1.998309030898054E-5 4.31001286711185 2.897548094802178E-4 68-69 5.994927092694162E-5 7.993236123592216E-5 1.998309030898054E-5 4.4479461479695885 2.997463546347081E-4 70-71 7.993236123592216E-5 7.993236123592216E-5 2.9974635463470813E-5 4.69538676372054 3.0973789978919837E-4 72-73 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 5.005384443683754 3.1972944494368865E-4 74-75 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 5.344507477772309 3.397125352526692E-4 76-77 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 5.837530281875477 3.397125352526692E-4 78-79 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 6.529194995195066 3.397125352526692E-4 80-81 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 7.294757176477265 3.397125352526692E-4 82-83 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 8.014448173955198 3.796787158706303E-4 84-85 7.993236123592216E-5 7.993236123592216E-5 3.996618061796108E-5 8.58838251071943 3.796787158706303E-4 86-87 7.993236123592216E-5 8.992390639041243E-5 3.996618061796108E-5 9.097022099899066 3.796787158706303E-4 88-89 7.993236123592216E-5 9.99154515449027E-5 3.996618061796108E-5 9.618330968334595 3.796787158706303E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6325 0.0 53.25066 1 GTATCAA 11655 0.0 42.78091 1 TCAACGC 14985 0.0 32.853634 4 ATCAACG 15165 0.0 32.526196 3 CAACGCA 15205 0.0 32.47132 5 TATCAAC 15725 0.0 31.488138 2 AACGCAG 15875 0.0 31.170046 6 GTACATG 22130 0.0 28.968456 1 TACATGG 22075 0.0 28.903168 2 ACATGGG 22470 0.0 27.513361 3 ACGCAGA 19530 0.0 25.191854 7 GAGTACA 15770 0.0 24.588242 1 CGCAGAG 20080 0.0 24.455143 8 CATGGGA 11865 0.0 23.727186 4 AGTACAT 16130 0.0 22.657692 2 CATGGGG 11065 0.0 21.777557 4 GCAGAGT 22565 0.0 21.11456 9 ATGGGAG 4995 0.0 19.54225 5 AGAGTAC 20175 0.0 18.9324 10-11 ATGGGAT 5250 0.0 18.682875 5 >>END_MODULE