FastQCFastQC Report
Fri 27 May 2016
SRR939838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294674
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89400.16884892252100886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65060.12287819797781696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65400.050.7087821
GTATCAA120550.043.157851
TCAACGC152200.033.503854
ATCAACG153900.033.2862743
CAACGCA154750.032.9526985
TATCAAC158950.032.4654962
AACGCAG162950.031.4685576
GTACATG230950.030.7968031
TACATGG234250.030.1624262
ACATGGG241650.028.1875743
ACGCAGA193100.026.1412437
CGCAGAG196700.025.4949078
CATGGGA123550.024.8628254
GAGTACA165650.024.2230831
AGTACAT168400.022.2634352
CATGGGG124500.022.1037834
GCAGAGT222100.021.4142659
GTATAGG13400.021.0629061
AGAGTAC195150.019.10376410-11
CAGAGTA205650.018.74599510-11
Produced by FastQC (version 0.11.5)