##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939838_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5294674 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.034997999876857 31.0 30.0 33.0 26.0 34.0 2 30.078984466276864 31.0 30.0 33.0 26.0 34.0 3 30.041256931021625 31.0 30.0 34.0 25.0 34.0 4 33.687998732310994 35.0 33.0 37.0 28.0 37.0 5 33.54232196354299 35.0 33.0 37.0 28.0 37.0 6 33.513947789797825 35.0 33.0 37.0 28.0 37.0 7 33.48420865949443 35.0 33.0 37.0 28.0 37.0 8 33.321373516103165 35.0 33.0 37.0 26.0 37.0 9 34.62801977987691 37.0 34.0 39.0 27.0 39.0 10-11 34.41880472716545 37.0 33.0 39.0 27.0 39.0 12-13 34.31831837049835 37.0 33.0 39.0 27.0 39.0 14-15 34.93168795661451 38.0 33.0 40.0 25.5 41.0 16-17 34.70165065875633 38.0 33.0 40.0 25.0 41.0 18-19 34.67286399502595 38.0 33.0 40.0 25.0 41.0 20-21 34.761058754514444 38.0 33.0 40.0 25.0 41.0 22-23 34.65841674104959 38.0 33.0 40.0 25.0 41.0 24-25 34.482237905487665 38.0 33.0 40.0 24.0 41.0 26-27 34.38607816080839 38.0 33.0 40.0 24.0 41.0 28-29 34.26789959117407 38.0 33.0 40.0 23.5 41.0 30-31 34.0985838788186 38.0 33.0 40.0 23.5 41.0 32-33 33.83780568926434 38.0 32.0 40.0 21.5 41.0 34-35 33.57606983923845 38.0 32.0 40.0 19.0 41.0 36-37 33.373701194823326 37.0 31.0 40.0 19.0 41.0 38-39 33.09780611610837 37.0 31.0 40.0 18.0 41.0 40-41 32.80066459238095 37.0 31.0 40.0 16.0 41.0 42-43 32.48791294799264 37.0 30.0 40.0 15.0 41.0 44-45 32.10482883365435 37.0 30.0 39.0 12.5 41.0 46-47 31.748028868255155 36.0 30.0 39.0 9.0 40.5 48-49 31.415159837980582 36.0 29.5 39.0 8.5 40.0 50-51 30.653537970420842 35.0 28.5 38.5 8.0 40.0 52-53 30.751531161314183 35.0 28.5 38.5 7.0 40.0 54-55 30.828116990772237 35.0 28.5 39.0 4.0 40.0 56-57 30.584287153467805 35.0 28.0 39.0 2.0 40.0 58-59 30.024616624177426 35.0 28.0 38.0 2.0 40.0 60-61 29.510918141513528 34.0 26.5 38.0 2.0 40.0 62-63 29.05775643599587 34.0 26.0 38.0 2.0 40.0 64-65 28.515530040187556 34.0 26.0 37.0 2.0 39.0 66-67 27.970567309715385 33.0 25.0 36.5 2.0 39.0 68-69 27.4222202349002 32.5 24.0 36.0 2.0 39.0 70-71 26.864043470853918 32.0 22.5 35.5 2.0 38.5 72-73 26.38186732554261 32.0 22.0 35.0 2.0 38.0 74-75 25.784902715445746 31.5 20.5 35.0 2.0 37.0 76-77 25.143141674067184 31.0 18.5 34.5 2.0 36.5 78-79 24.497975323882073 31.0 16.0 34.0 2.0 36.0 80-81 23.825680485710734 30.0 9.0 34.0 2.0 35.5 82-83 23.079070684994015 29.5 4.5 34.0 2.0 35.0 84-85 22.335706693178842 29.0 2.0 34.0 2.0 35.0 86-87 21.53891608812932 29.0 2.0 33.5 2.0 35.0 88-89 20.661301337910512 27.5 2.0 33.0 2.0 34.5 90-91 19.67911707123045 26.0 2.0 32.5 2.0 34.0 92-93 18.679260139528893 25.0 2.0 32.0 2.0 34.0 94-95 17.284387197398743 21.5 2.0 32.0 2.0 34.0 96-97 14.734937316254031 2.0 2.0 30.5 2.0 34.0 98-99 13.342729221855773 2.0 2.0 30.0 2.0 34.0 100-101 12.070715779668399 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7665168963940516 1101 2 0.7513539179567772 1101 3 0.9762182748489963 1101 4 0.9574708994580519 1101 5 0.9763846101704701 1101 6 1.0523764199693133 1101 7 0.9297677637778392 1101 8 0.6979841713389163 1101 9 0.899804717523935 1101 10-11 0.9232634414819358 1101 12-13 0.7353272129852471 1101 14-15 0.8723174493735684 1101 16-17 0.9181456111166497 1101 18-19 0.8489475575755066 1101 20-21 0.9540903620061414 1101 22-23 0.9076937457616481 1101 24-25 0.9520518862121676 1101 26-27 0.9951525546599811 1101 28-29 1.1901434966880657 1101 30-31 1.1351244790233537 1101 32-33 1.086180086831341 1101 34-35 0.8538896561481479 1101 36-37 0.8760799549301623 1101 38-39 0.8400330052063083 1101 40-41 0.9499982762846741 1101 42-43 0.9585171266955683 1101 44-45 0.8872996921336949 1101 46-47 0.8718889710266069 1101 48-49 0.7954094655986914 1101 50-51 0.888011485309935 1101 52-53 0.9282422845987846 1101 54-55 0.9124021263558291 1101 56-57 0.7015377029228809 1101 58-59 0.4708187655802796 1101 60-61 0.6956255923975903 1101 62-63 0.48900905952674734 1101 64-65 0.6763084742452996 1101 66-67 0.3727984297389426 1101 68-69 0.38071200006726613 1101 70-71 0.43756518982884884 1101 72-73 0.22270418424531258 1101 74-75 0.055800282158291026 1101 76-77 -0.24381390169934036 1101 78-79 -0.11229939441296466 1101 80-81 -0.4265577701850489 1101 82-83 -0.5227509182131769 1101 84-85 -0.664986063045415 1101 86-87 -0.5955485543261254 1101 88-89 -0.6723713198574757 1101 90-91 -0.6175240687095709 1101 92-93 -1.0588110759847638 1101 94-95 -0.676068402209399 1101 96-97 0.8961256139397165 1101 98-99 0.9726946695820065 1101 100-101 0.3461775310437343 1102 1 0.7481976767067842 1102 2 0.8150206797842188 1102 3 0.8569224029065268 1102 4 0.9315760973805496 1102 5 0.989942287479586 1102 6 0.9965850179904407 1102 7 0.8337637174128218 1102 8 0.8452329248238826 1102 9 0.9724618599846622 1102 10-11 0.846557831775371 1102 12-13 0.8153986970854774 1102 14-15 0.993916310135134 1102 16-17 1.017803116421625 1102 18-19 0.9980019131569833 1102 20-21 0.9485249283231809 1102 22-23 0.875931840539252 1102 24-25 0.92950887361269 1102 26-27 1.1051841258932882 1102 28-29 1.2872919950048143 1102 30-31 1.0453772885435768 1102 32-33 1.1722750797068997 1102 34-35 1.089705620538119 1102 36-37 0.9335649103482524 1102 38-39 0.9014228458197167 1102 40-41 1.0785277564335587 1102 42-43 0.9489267707003748 1102 44-45 0.8271831699390262 1102 46-47 0.9538537200565287 1102 48-49 0.6562099260703143 1102 50-51 0.9130967200718416 1102 52-53 0.8554180151539263 1102 54-55 0.8616285281307796 1102 56-57 0.7925809158645194 1102 58-59 0.6604095083850972 1102 60-61 0.6672987248113529 1102 62-63 0.5284392553154866 1102 64-65 0.2737941696470152 1102 66-67 0.2633479138316055 1102 68-69 0.2218314187092183 1102 70-71 0.37747284323565466 1102 72-73 0.31501398848180173 1102 74-75 0.22699990913926982 1102 76-77 0.027158853028460328 1102 78-79 -0.19079084528961232 1102 80-81 -0.33208834895735606 1102 82-83 -0.5470133416343295 1102 84-85 -0.6512873263572345 1102 86-87 -0.7744099549721817 1102 88-89 -0.6959831042649292 1102 90-91 -0.8170078880846106 1102 92-93 -0.9387766919727838 1102 94-95 -0.7461013356852924 1102 96-97 0.3924025713120347 1102 98-99 0.5020379635434438 1102 100-101 0.15623025067276686 1103 1 0.7143272564438732 1103 2 0.6418629818849304 1103 3 0.7932433378283079 1103 4 0.6697678156838833 1103 5 0.8522983153309909 1103 6 0.8387928389226076 1103 7 0.7373626804246101 1103 8 0.711579124641311 1103 9 0.9283110340833645 1103 10-11 0.765885525350896 1103 12-13 0.7009349278513994 1103 14-15 0.7982829756648897 1103 16-17 0.904049666384914 1103 18-19 0.7966111449197655 1103 20-21 0.7521854077738368 1103 22-23 0.7770892104700238 1103 24-25 0.8508987139462363 1103 26-27 1.0129320135719624 1103 28-29 0.996505210885104 1103 30-31 0.7914082662278688 1103 32-33 0.7567800341614159 1103 34-35 0.8625458093121594 1103 36-37 0.6752449804816862 1103 38-39 0.6868458291447084 1103 40-41 0.7240734129432838 1103 42-43 0.6573987434667714 1103 44-45 0.6055323399418455 1103 46-47 0.6445842639793682 1103 48-49 0.6565432006215239 1103 50-51 0.8516382193348058 1103 52-53 0.960195374870306 1103 54-55 0.7727096639980715 1103 56-57 0.5266316592374949 1103 58-59 0.4229373063981896 1103 60-61 0.6749109047162349 1103 62-63 0.5582370088055875 1103 64-65 0.4457529095091495 1103 66-67 0.3590486665357453 1103 68-69 0.1870416030403348 1103 70-71 0.35932285006460774 1103 72-73 0.08349697693930835 1103 74-75 -0.08100857350885704 1103 76-77 -0.11762167392252465 1103 78-79 -0.1262319286938549 1103 80-81 -0.3664658209158276 1103 82-83 -0.3455554381515 1103 84-85 -0.5482957530034689 1103 86-87 -0.6889528939007796 1103 88-89 -0.615256024714391 1103 90-91 -0.7479690994770039 1103 92-93 -0.751533196644548 1103 94-95 -0.4781350242483491 1103 96-97 0.8934611522688005 1103 98-99 0.9018455166197565 1103 100-101 0.5700377365765998 1104 1 0.5831019659717604 1104 2 0.6118472336245269 1104 3 0.6924718569004327 1104 4 0.9037705272746805 1104 5 0.8069364209909722 1104 6 0.9309651226968256 1104 7 0.8695073081957858 1104 8 0.8901608492204645 1104 9 1.0528473040328592 1104 10-11 1.0312227114099386 1104 12-13 0.8999435755003518 1104 14-15 0.999784575358106 1104 16-17 0.901118599617341 1104 18-19 0.9646837450984904 1104 20-21 0.8204158218814044 1104 22-23 0.7719633305532483 1104 24-25 0.9694677170774995 1104 26-27 1.1790622131093045 1104 28-29 1.1260394088075714 1104 30-31 1.0540841480470888 1104 32-33 1.095486517853189 1104 34-35 1.0331032565463616 1104 36-37 0.9249415154511311 1104 38-39 0.7332891249331297 1104 40-41 0.7850557582470117 1104 42-43 0.6356648865024752 1104 44-45 0.7196879540164147 1104 46-47 0.5609905675301832 1104 48-49 0.3449413847905234 1104 50-51 0.7076585622510052 1104 52-53 0.7258382347920183 1104 54-55 0.6552211004044395 1104 56-57 0.6697312036053091 1104 58-59 0.5775680765979985 1104 60-61 0.7436615700378439 1104 62-63 0.49354945451997523 1104 64-65 0.4963714145630256 1104 66-67 0.49599618596741024 1104 68-69 0.23151884230800945 1104 70-71 0.43523002453655124 1104 72-73 0.45826501394166286 1104 74-75 0.3081449101115119 1104 76-77 0.20776174014243765 1104 78-79 0.29297278700593665 1104 80-81 0.18815751050746243 1104 82-83 0.2704746906034572 1104 84-85 0.17064539558637293 1104 86-87 0.1493936214100593 1104 88-89 0.017637521833467673 1104 90-91 -0.03722535435513663 1104 92-93 0.10734560659026471 1104 94-95 0.4245532918705841 1104 96-97 1.6859435311325282 1104 98-99 1.7299089565579795 1104 100-101 1.5155609718515617 1105 1 0.5944277213297511 1105 2 0.7938270867438284 1105 3 0.7084868761668446 1105 4 0.6599501580470957 1105 5 0.7430371619541987 1105 6 0.7715378958730668 1105 7 0.7180169692455394 1105 8 0.7196433194272629 1105 9 0.9318636113089767 1105 10-11 0.8970619543725888 1105 12-13 0.5365196385898656 1105 14-15 0.8639493603479877 1105 16-17 0.8171542122447022 1105 18-19 0.929383263118936 1105 20-21 0.7985015304587577 1105 22-23 0.6189240233331716 1105 24-25 0.7826368573783 1105 26-27 0.993331922354912 1105 28-29 0.9911529817863993 1105 30-31 1.0941397180208625 1105 32-33 1.059498084329654 1105 34-35 1.1886069456610002 1105 36-37 0.990314742030229 1105 38-39 1.0557105008278356 1105 40-41 0.8410163848015912 1105 42-43 0.7608942240375711 1105 44-45 0.9837541407016843 1105 46-47 0.8748925830486911 1105 48-49 0.847317873299378 1105 50-51 0.8368214689951792 1105 52-53 0.8827299345789186 1105 54-55 0.9307381832289003 1105 56-57 0.803507291873018 1105 58-59 0.7854955819659821 1105 60-61 0.9683243214769526 1105 62-63 0.8121274574747837 1105 64-65 0.8128044560279797 1105 66-67 0.6049542910041588 1105 68-69 0.571292235783357 1105 70-71 0.6222716504113599 1105 72-73 0.5765751810814557 1105 74-75 0.5239802207261839 1105 76-77 0.479434612614817 1105 78-79 0.3180835679130247 1105 80-81 0.33213167396934296 1105 82-83 0.2731535023518745 1105 84-85 0.24007818701401007 1105 86-87 0.0509303843975033 1105 88-89 0.17679773629751594 1105 90-91 0.16533700825256403 1105 92-93 0.17980644553350444 1105 94-95 0.4572488986179195 1105 96-97 1.6283768352657635 1105 98-99 1.7280228601052645 1105 100-101 1.2207922193842489 1106 1 0.9871058248803379 1106 2 1.0732194923095122 1106 3 1.0009949644048355 1106 4 1.0302952195322277 1106 5 1.1152613003178757 1106 6 1.1945108429036537 1106 7 1.018982934618812 1106 8 1.1430182247616045 1106 9 1.3205680041765007 1106 10-11 1.208776898097348 1106 12-13 1.2654192003506992 1106 14-15 1.5303838431576864 1106 16-17 1.5710389284030342 1106 18-19 1.516376085102067 1106 20-21 1.3656080576418361 1106 22-23 1.087985492935296 1106 24-25 1.324861119410393 1106 26-27 1.6008921501320046 1106 28-29 1.4974048696171423 1106 30-31 1.4214214056978562 1106 32-33 1.5007657423999987 1106 34-35 1.6126151663032928 1106 36-37 1.7824206742388 1106 38-39 1.5979115930068915 1106 40-41 1.6000619565411682 1106 42-43 1.496987548826283 1106 44-45 1.5947690672638473 1106 46-47 1.5687855885859392 1106 48-49 1.4908569103495033 1106 50-51 1.6280317938124362 1106 52-53 1.7195029215716566 1106 54-55 1.660905166508286 1106 56-57 1.6309409766619183 1106 58-59 1.6372519323211954 1106 60-61 1.8609411660212203 1106 62-63 1.789192430631907 1106 64-65 1.8222694398550985 1106 66-67 1.7141123398875528 1106 68-69 1.6886488535787088 1106 70-71 1.8192449521977423 1106 72-73 1.9006497225071044 1106 74-75 1.781949481790857 1106 76-77 1.7759889229300825 1106 78-79 1.605317845289349 1106 80-81 1.696739761207919 1106 82-83 1.8252800994090421 1106 84-85 1.686711868006583 1106 86-87 1.727523706346151 1106 88-89 1.8087670896820995 1106 90-91 1.8408886035427834 1106 92-93 1.9534172627523745 1106 94-95 2.2382885387313216 1106 96-97 3.761066988577932 1106 98-99 3.9057946989230405 1106 100-101 3.205737800607473 1107 1 0.5229068494037215 1107 2 0.5825809531687369 1107 3 0.6156241973571355 1107 4 0.21047879477843878 1107 5 0.5705419693006277 1107 6 0.5875365425940515 1107 7 0.6233929296675242 1107 8 0.4187993342033707 1107 9 0.6129909777555937 1107 10-11 0.6579466792219009 1107 12-13 0.6756048208701984 1107 14-15 0.7170225306845097 1107 16-17 0.734236202298824 1107 18-19 0.6706454769224806 1107 20-21 0.5657589191551295 1107 22-23 0.5616051028183051 1107 24-25 0.581814915562795 1107 26-27 0.8066427585094829 1107 28-29 0.7876993332889981 1107 30-31 0.8209372217390225 1107 32-33 0.8369985086141938 1107 34-35 0.8131432879951674 1107 36-37 0.5592651697388291 1107 38-39 0.5934450630157002 1107 40-41 0.6317172862513161 1107 42-43 0.5989361406501246 1107 44-45 0.6285409906020334 1107 46-47 0.5984049368782536 1107 48-49 0.456450799795018 1107 50-51 0.5426434076947722 1107 52-53 0.7052947092054076 1107 54-55 0.6074550330690087 1107 56-57 0.42840341919202984 1107 58-59 0.30045028790303974 1107 60-61 0.5521014254003163 1107 62-63 0.4466115326615352 1107 64-65 0.45318267618495156 1107 66-67 0.4485631116634643 1107 68-69 0.4037168396753934 1107 70-71 0.47210840039797475 1107 72-73 0.29083626228241855 1107 74-75 0.2333127981140528 1107 76-77 0.26825696005397504 1107 78-79 0.17119733177290186 1107 80-81 -0.026723952888481506 1107 82-83 0.05252810104724759 1107 84-85 -0.010163435330991177 1107 86-87 0.10223498926447405 1107 88-89 0.06517757797966084 1107 90-91 -0.028626809260003938 1107 92-93 0.10058261931990131 1107 94-95 0.5671885196901023 1107 96-97 1.889985613548541 1107 98-99 2.058715600612283 1107 100-101 1.6152740725732802 1108 1 0.505427229243697 1108 2 0.4734357966393006 1108 3 0.5086986919366936 1108 4 0.541237235845152 1108 5 0.6335228151672823 1108 6 0.5873259521663812 1108 7 0.5997136802996366 1108 8 0.617842290006493 1108 9 0.5894736238340457 1108 10-11 0.5296906035839157 1108 12-13 0.36536182130304695 1108 14-15 0.48620543367850644 1108 16-17 0.6135806415026934 1108 18-19 0.520352514801985 1108 20-21 0.5099800815159412 1108 22-23 0.32495324748487775 1108 24-25 0.5253473307805976 1108 26-27 0.5443133668061009 1108 28-29 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2311 94-95 2.785671766104368 2311 96-97 4.316893996369805 2311 98-99 4.591014998191708 2311 100-101 4.000171289595981 2312 1 0.02013050946217021 2312 2 -0.4542265231271969 2312 3 -0.863233736940753 2312 4 -0.341010468565635 2312 5 -1.9777204828396577 2312 6 -0.5318751796561827 2312 7 0.02779252580304359 2312 8 0.16664195991767627 2312 9 0.35967042441530594 2312 10-11 0.1767786032582066 2312 12-13 0.2698167284773163 2312 14-15 0.3908257375857005 2312 16-17 -0.27864117097929864 2312 18-19 0.05402572374131864 2312 20-21 0.3803965367879698 2312 22-23 0.32895330385677823 2312 24-25 0.2755031364603795 2312 26-27 0.6445052270163032 2312 28-29 0.6989133921688477 2312 30-31 0.34758522677061876 2312 32-33 -0.39016510100817214 2312 34-35 0.14423118229293408 2312 36-37 0.48619496891856784 2312 38-39 0.6548556410441293 2312 40-41 0.7768217606062393 2312 42-43 0.7582222373437304 2312 44-45 0.7483342723873037 2312 46-47 0.3224287233009733 2312 48-49 0.556334335060594 2312 50-51 0.5795368605064191 2312 52-53 0.3355411201834606 2312 54-55 0.47915149557424286 2312 56-57 0.6050126417998811 2312 58-59 0.7352246855257931 2312 60-61 0.7739366445396101 2312 62-63 0.7926401061000341 2312 64-65 0.6614646193808049 2312 66-67 0.8597627160475021 2312 68-69 0.7057701383283757 2312 70-71 0.7878998652890168 2312 72-73 0.7973266408740542 2312 74-75 0.8904131009602132 2312 76-77 0.8505965712717583 2312 78-79 0.864424640237452 2312 80-81 0.812436744691361 2312 82-83 1.0075814221487498 2312 84-85 0.9556001124796509 2312 86-87 1.1335154318030582 2312 88-89 1.0924923985951267 2312 90-91 1.2336137994289302 2312 92-93 1.3871016909014386 2312 94-95 1.945448710011764 2312 96-97 3.46569767529345 2312 98-99 3.816258253447531 2312 100-101 3.292344745987032 2313 1 -0.03223768570180141 2313 2 -0.5460455542228857 2313 3 -0.843307057898901 2313 4 -0.07272648432175544 2313 5 -0.2553592841046779 2313 6 0.10172236492077502 2313 7 -0.010093339388312472 2313 8 0.2870928908875925 2313 9 0.31971292873246426 2313 10-11 -0.5696970214662826 2313 12-13 0.07347255219561788 2313 14-15 0.2788090674814896 2313 16-17 -0.2669014891954973 2313 18-19 0.03755037199888278 2313 20-21 -2.227885903085209 2313 22-23 -0.21774204614665393 2313 24-25 0.2871379855518583 2313 26-27 0.3228166636500873 2313 28-29 0.06005816264148933 2313 30-31 0.277039775763825 2313 32-33 0.5792522516522851 2313 34-35 0.4416848157852016 2313 36-37 -0.3289795601368368 2313 38-39 0.34623748379433295 2313 40-41 0.3676797937604164 2313 42-43 0.45292681515169875 2313 44-45 0.6918882813815586 2313 46-47 0.645103055202668 2313 48-49 0.4189527031132023 2313 50-51 0.7400304676710867 2313 52-53 0.6157905019296592 2313 54-55 0.6598708614268176 2313 56-57 0.6499094065581019 2313 58-59 0.7660605363603068 2313 60-61 0.49011018681543206 2313 62-63 0.5223340957428917 2313 64-65 0.3576023529826742 2313 66-67 0.7425358800915198 2313 68-69 0.5906203040385947 2313 70-71 0.9353842977289943 2313 72-73 0.686881702871311 2313 74-75 0.883550003483375 2313 76-77 1.0245923638094396 2313 78-79 0.855534524614388 2313 80-81 0.9789998424715094 2313 82-83 1.104435771561736 2313 84-85 1.0673049384990883 2313 86-87 1.0610371793735176 2313 88-89 1.210895585003449 2313 90-91 1.6150199047231624 2313 92-93 1.7820643884090082 2313 94-95 2.062672512833789 2313 96-97 3.425758883936668 2313 98-99 3.621591526623705 2313 100-101 3.1537266064305154 2314 1 -0.1315432796043332 2314 2 -0.18601507145306684 2314 3 -1.163149282400596 2314 4 -0.21328465058174118 2314 5 -0.4583857531443982 2314 6 -0.43575510508313187 2314 7 -0.3123875501558331 2314 8 -1.8303292627751873 2314 9 -1.5799209803017007 2314 10-11 -0.9022567496533185 2314 12-13 -0.5765369203906303 2314 14-15 -0.6576055770732339 2314 16-17 -0.7948959397485922 2314 18-19 -1.7543251708140275 2314 20-21 -1.2760718487444933 2314 22-23 -1.9328609172434028 2314 24-25 -1.725606396358394 2314 26-27 -1.8816319656685891 2314 28-29 -2.83240753056479 2314 30-31 -1.480908194863396 2314 32-33 -1.256090788293399 2314 34-35 -1.5420760605123647 2314 36-37 -3.2204078810957952 2314 38-39 -3.5938608771492433 2314 40-41 -4.371266162494944 2314 42-43 -3.1535704056716547 2314 44-45 -3.309292833704067 2314 46-47 -3.2739004478093534 2314 48-49 -1.569971082582672 2314 50-51 -1.053876019262848 2314 52-53 -2.5539422120954463 2314 54-55 -1.861911442891433 2314 56-57 -1.7404918799323497 2314 58-59 -3.8384679260576355 2314 60-61 -2.125105503146372 2314 62-63 -2.1677198286560717 2314 64-65 -1.5126806298755717 2314 66-67 -2.545770838815816 2314 68-69 -2.392608574257057 2314 70-71 -1.91693989577794 2314 72-73 -1.1277681257060514 2314 74-75 -1.4983045482310935 2314 76-77 -0.5838783230847504 2314 78-79 -0.35101954444986205 2314 80-81 -0.16856622452340275 2314 82-83 -0.09615817041081698 2314 84-85 -0.08570751569341795 2314 86-87 0.1448459975179297 2314 88-89 0.22781385306097235 2314 90-91 0.9351155161356672 2314 92-93 1.141194434740072 2314 94-95 1.505442187630809 2314 96-97 3.0749513221027573 2314 98-99 3.201896569347676 2314 100-101 2.8646787164952254 2315 1 -2.311688309968048 2315 2 -1.842018271461484 2315 3 -1.5588066639592064 2315 4 -1.7006635175070883 2315 5 -1.8598190998666801 2315 6 -3.1962344175507766 2315 7 -2.2175013298361286 2315 8 -1.2270417376965597 2315 9 -1.1134065261030344 2315 10-11 -3.366604707855423 2315 12-13 -1.84200928751374 2315 14-15 -1.1612998628815205 2315 16-17 -1.963924025697061 2315 18-19 -1.3021828563785647 2315 20-21 -0.9585977736162619 2315 22-23 -1.1407761023979646 2315 24-25 -2.9789895381707296 2315 26-27 -4.32729557138979 2315 28-29 -5.950118069357934 2315 30-31 -8.582566518928896 2315 32-33 -7.001319748010403 2315 34-35 -6.35228367433389 2315 36-37 -3.905696006592869 2315 38-39 -6.447845133256635 2315 40-41 -4.1342432208280755 2315 42-43 -3.9979125488833205 2315 44-45 -3.1092002280412885 2315 46-47 -2.3147722884856705 2315 48-49 -3.1481787437347712 2315 50-51 -2.9192586058067675 2315 52-53 -2.192570298274795 2315 54-55 -3.6638274684522543 2315 56-57 -3.429864543987456 2315 58-59 -2.2487520255700986 2315 60-61 -2.5166519872512865 2315 62-63 -3.0242967803735645 2315 64-65 -2.239979030980873 2315 66-67 -2.8326461321867953 2315 68-69 -2.0916909319531705 2315 70-71 -2.467751397029925 2315 72-73 -1.7817610751183288 2315 74-75 -2.181187328298048 2315 76-77 -1.5025588852718954 2315 78-79 -1.5939214337541756 2315 80-81 -1.146227797980167 2315 82-83 -0.7252718336245536 2315 84-85 -0.6953204542299254 2315 86-87 -1.311086402240182 2315 88-89 -1.7024099493553138 2315 90-91 -1.2838098470799792 2315 92-93 -0.5225799417069013 2315 94-95 0.09968221417184608 2315 96-97 1.579672546638287 2315 98-99 1.3577151929528473 2315 100-101 1.0254674189057944 2316 1 -3.587564497038173 2316 2 -2.3487709109638644 2316 3 -2.330738812034035 2316 4 -3.1601469259846233 2316 5 -2.618230718239527 2316 6 -6.008665771353243 2316 7 -3.540984414420958 2316 8 -4.584429144929377 2316 9 -6.595834656710778 2316 10-11 -5.645906554695589 2316 12-13 -3.6810963075746983 2316 14-15 -5.0179628459429395 2316 16-17 -4.4915125369712 2316 18-19 -2.583614406898974 2316 20-21 -3.289733947027539 2316 22-23 -4.073124731923755 2316 24-25 -2.737785425953465 2316 26-27 -3.9142411928937477 2316 28-29 -3.7942630393392633 2316 30-31 -3.4830115246418067 2316 32-33 -3.36128707555881 2316 34-35 -5.5498162913211075 2316 36-37 -5.626735518516618 2316 38-39 -5.669617063927802 2316 40-41 -5.110212921962891 2316 42-43 -4.363910580034361 2316 44-45 -3.6009082152680776 2316 46-47 -3.7975409109755915 2316 48-49 -4.306439723747143 2316 50-51 -5.45590744392678 2316 52-53 -3.3107224743550425 2316 54-55 -3.6096351593435436 2316 56-57 -2.9241219661887534 2316 58-59 -3.339431595675098 2316 60-61 -3.378441098876344 2316 62-63 -2.51894757424299 2316 64-65 -2.92141842196499 2316 66-67 -3.652509696559843 2316 68-69 -2.9105084590304635 2316 70-71 -2.3805852664975014 2316 72-73 -2.5051679126383917 2316 74-75 -3.4269975042503162 2316 76-77 -3.63228813852038 2316 78-79 -3.558859631347154 2316 80-81 -3.724515052856912 2316 82-83 -2.807764315753225 2316 84-85 -2.5835989031935647 2316 86-87 -2.192773853038627 2316 88-89 -2.3769471316307147 2316 90-91 -1.9433530254479265 2316 92-93 -1.5122428264896186 2316 94-95 -1.4899722361726404 2316 96-97 0.2654735567609894 2316 98-99 0.5481666864728894 2316 100-101 0.19246881429643103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45769.0 3 15551.0 4 15201.0 5 17303.0 6 19528.0 7 23149.0 8 28067.0 9 33637.0 10 39076.0 11 45220.0 12 50821.0 13 55610.0 14 59527.0 15 64941.0 16 70839.0 17 75181.0 18 75424.0 19 77981.0 20 85290.0 21 94106.0 22 105082.0 23 117727.0 24 131597.0 25 146760.0 26 166257.0 27 185531.0 28 209258.0 29 235108.0 30 264355.0 31 300233.0 32 339546.0 33 386519.0 34 433625.0 35 468477.0 36 446411.0 37 302194.0 38 63327.0 39 446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54991919877253 17.88984181264024 11.94918353508112 24.611055453506108 2 16.817026062526388 19.700443467407236 37.82797972450299 25.654550745563387 3 20.08999015112203 23.59226099072753 28.412216390893043 27.905532467257398 4 13.293217240652108 14.814167543792456 35.39949178717098 36.49312342838446 5 14.374394196007675 37.197523544202774 33.43648431789189 14.991597941897664 6 34.47545176045963 34.19174093405752 16.772282013970504 14.560525291512356 7 30.592168102944438 31.360896046677105 19.935763751629928 18.111172098748526 8 28.258439504902555 33.611510410361944 19.345399316063432 18.784650768672073 9 27.863329442414496 15.055113153480953 18.937293028937503 38.14426437516705 10-11 26.322133855983804 25.453770296516947 26.611906772940696 21.61218907455855 12-13 26.593535088271803 22.600767291436263 27.19216464667729 23.61353297361464 14-15 23.60376011609915 24.144407536988545 24.60514081239334 27.64669153451897 16-17 23.734636215891015 27.344618375205894 25.40801607155792 23.51272933734517 18-19 23.747104154713945 26.077712220273213 27.1242929805675 23.05089064444534 20-21 24.184394962249417 25.48494092229081 27.158432483685818 23.172231631773954 22-23 23.976419264634508 25.368556628597172 27.09779415334102 23.5572299534273 24-25 23.678831740974434 25.622276846709163 27.271785131682407 23.427106280633996 26-27 23.36918390458104 25.87672369284703 27.187068618343563 23.567023784228365 28-29 23.473711469396996 25.991494096561517 27.22159796043072 23.313196473610763 30-31 23.47351586621247 25.86662040261649 27.01859335896975 23.641270372201287 32-33 23.21064018904904 25.963074786037804 27.1243005760082 23.70198444890496 34-35 23.723266126903255 25.913942538152178 27.016888376162225 23.34590295878234 36-37 23.759882349401614 25.680219557936052 26.962633030080035 23.597265062582302 38-39 23.45999376999157 25.85503648491267 27.08019262307957 23.60477712201619 40-41 23.580496154829405 25.673754007100904 27.32426044258868 23.421489395481014 42-43 23.37844882805367 25.663803307166035 27.116178470021772 23.84156939475853 44-45 23.240296791177446 25.774709203442676 27.28762812784953 23.697365877530352 46-47 23.57010572909771 25.94771624713612 26.89022028322819 23.591957740537982 48-49 23.355214554187516 26.078189892876956 26.7804864981878 23.78610905474773 50-51 23.326502974732406 26.077618055624583 26.825786094753116 23.77009287488989 52-53 23.435906734696484 25.91002508745575 26.858500405499264 23.795567772348498 54-55 23.131111009275422 26.067917629739824 26.902217034493386 23.898754326491364 56-57 23.06011096546925 26.243231250250837 26.757536966403677 23.93912081787624 58-59 23.075437092407224 25.829023189498233 26.902954610460988 24.192585107633548 60-61 22.921587712602907 26.0756591827971 26.994221830168254 24.008531274431746 62-63 22.965833843014245 25.905522569276236 27.133128148919678 23.995515438789848 64-65 22.818559609202868 25.919261094295553 27.015236940441127 24.24694235606045 66-67 22.746638799201367 26.152087335213363 26.780032454017167 24.3212414115681 68-69 23.029529268420525 25.722961180875544 27.01319793842173 24.234311612282198 70-71 22.888619612569467 25.673699358004004 26.90396855200775 24.533712477418778 72-73 22.83701125847547 25.913082918669943 26.719059447642994 24.53084637521159 74-75 22.78337654164796 26.06357344547332 26.699008769361505 24.454041243517214 76-77 22.926402109724574 25.750925215228698 26.88582812360794 24.436844551438785 78-79 22.894443843299882 25.85260522325515 26.8156672094008 24.437283724044164 80-81 23.021486426548478 25.912087943298665 26.64691795622622 24.41950767392664 82-83 23.01783141116591 25.751309596175854 26.738454428003283 24.492404564654947 84-85 22.821263689824807 25.809372836801565 26.671211444130805 24.698152029242824 86-87 23.04622182588332 25.677394539047643 26.719456257684733 24.55692737738431 88-89 23.040614827552524 25.61338509521354 26.492668833760014 24.85333124347392 90-91 22.935849633086942 25.664671122306526 26.6677816651281 24.731697579478425 92-93 23.269267267145036 25.353786388284 26.562113825216965 24.814832519353995 94-95 23.113996686989832 25.125260239987345 26.833735224431877 24.92700784859095 96-97 23.430008393677003 24.80807986335884 26.638135269076162 25.123776473887986 98-99 23.399063934009934 24.918037631508483 26.413312736459055 25.269585698022535 100-101 23.401173805694956 24.85987689780032 26.63903864487547 25.099910651629266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6408.0 1 3663.5 2 907.5 3 901.0 4 800.0 5 740.5 6 1028.5 7 1646.0 8 2372.0 9 2718.0 10 2434.0 11 2169.0 12 2268.0 13 2427.5 14 2609.5 15 2853.0 16 3161.0 17 3497.0 18 3856.5 19 4374.0 20 5097.5 21 6003.5 22 7130.5 23 8801.0 24 10728.5 25 13163.5 26 16813.0 27 21301.0 28 25703.5 29 30925.0 30 37815.5 31 46620.0 32 59048.5 33 68328.5 34 74246.0 35 84363.0 36 99107.0 37 112563.0 38 122370.5 39 130417.0 40 136373.5 41 141299.0 42 146549.5 43 156204.5 44 167453.5 45 177393.5 46 185932.0 47 194922.0 48 202926.5 49 207049.5 50 210191.5 51 213952.0 52 218099.0 53 220607.0 54 219492.5 55 210079.5 56 195659.5 57 180986.0 58 166596.5 59 148385.5 60 124931.5 61 102512.0 62 82582.0 63 64367.0 64 48639.5 65 36620.5 66 27468.5 67 20759.0 68 15775.0 69 11843.5 70 8754.0 71 6152.5 72 4386.0 73 3275.0 74 2387.0 75 1744.0 76 1231.0 77 881.5 78 613.0 79 417.5 80 293.0 81 189.0 82 141.5 83 105.5 84 63.5 85 42.5 86 41.5 87 31.5 88 21.0 89 21.5 90 17.5 91 10.0 92 8.5 93 5.5 94 5.5 95 5.5 96 2.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12064954329577232 2 0.11564451371321445 3 0.1277132454236087 4 0.1420106318160476 5 0.13747777483561782 6 0.13689228080897897 7 0.14406930436132612 8 0.14418262578583685 9 0.15118966720141788 10-11 0.1468551227138819 12-13 0.14665681022098812 14-15 0.13455974815446617 16-17 0.13827102480719305 18-19 0.15526923848380467 20-21 0.18613043975889731 22-23 0.2036858171060201 24-25 0.22165670634301565 26-27 0.22412144732612432 28-29 0.22645397998063715 30-31 0.22578349488561522 32-33 0.22708669126748882 34-35 0.1984447012223982 36-37 0.18647040403242957 38-39 0.19967234998793126 40-41 0.18873683252264445 42-43 0.19054997531481635 44-45 0.19552667454124656 46-47 0.2154523583510524 48-49 0.23212949465821692 50-51 0.24754120839167812 52-53 0.26718358864020714 54-55 0.2613192049217761 56-57 0.23249778928787684 58-59 0.22628399784387102 60-61 0.20437518910512717 62-63 0.19495062396665025 64-65 0.1867159337855362 66-67 0.18240971965412792 68-69 0.18858573728996347 70-71 0.19150376397111513 72-73 0.2280782537319578 74-75 0.23994867294945826 76-77 0.256965773530155 78-79 0.24708792269363516 80-81 0.25150745824955417 82-83 0.24891995238989217 84-85 0.23458479218928305 86-87 0.22432920327106068 88-89 0.2270583609113611 90-91 0.2295797626067252 92-93 0.23602019689975248 94-95 0.24119520861907642 96-97 8.723284946344194 98-99 0.2999145934197271 100-101 0.31084066743297134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5294674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.405753364808376 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62769032268682 29.657984795920427 2 12.126648313411906 10.042260184138058 3 5.238580784109684 6.507221517854102 4 2.9553577525404227 4.894752568258526 5 1.8687510708093387 3.868852296907652 6 1.226526443153386 3.047115084037486 7 0.8689114764868228 2.518455400388541 8 0.6506184754400003 2.155147850292503 9 0.4949213912182499 1.8443333753775624 >10 2.6505843697977873 19.722986201754573 >50 0.18356772312165345 5.2480101037252185 >100 0.10088039156734671 7.432545622654292 >500 0.004952467631140992 1.4359079143661146 >1k 0.0019155753268411773 1.325619167873424 >5k 9.344269886991917E-5 0.29880791645146926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8940 0.16884892252100886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6506 0.12287819797781696 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.888690408512403E-5 0.0 0.0 0.0 0.0 6 1.888690408512403E-5 0.0 0.0 0.0 0.0 7 1.888690408512403E-5 0.0 0.0 1.888690408512403E-5 0.0 8 1.888690408512403E-5 0.0 0.0 1.888690408512403E-5 1.888690408512403E-5 9 1.888690408512403E-5 0.0 0.0 1.888690408512403E-5 1.888690408512403E-5 10-11 1.888690408512403E-5 0.0 0.0 1.888690408512403E-5 1.888690408512403E-5 12-13 1.888690408512403E-5 0.0 0.0 2.8330356127686045E-5 1.888690408512403E-5 14-15 1.888690408512403E-5 0.0 0.0 4.721726021281008E-5 2.8330356127686045E-5 16-17 2.8330356127686045E-5 0.0 0.0 1.1332142451074418E-4 5.6660712255372096E-5 18-19 3.777380817024806E-5 0.0 0.0 1.7942558880867828E-4 5.6660712255372096E-5 20-21 3.777380817024806E-5 0.0 0.0 4.060684378301667E-4 5.6660712255372096E-5 22-23 3.777380817024806E-5 0.0 0.0 9.254583001710775E-4 1.322083285958682E-4 24-25 3.777380817024806E-5 1.888690408512403E-5 0.0 0.0016053868472355427 2.2664284902148838E-4 26-27 3.777380817024806E-5 3.777380817024806E-5 0.0 0.002823592160726043 2.2664284902148838E-4 28-29 3.777380817024806E-5 3.777380817024806E-5 0.0 0.008234690181114078 2.3608630106405041E-4 30-31 3.777380817024806E-5 3.777380817024806E-5 0.0 0.03138059113743358 2.549732051491744E-4 32-33 3.777380817024806E-5 3.777380817024806E-5 0.0 0.08602040465569741 2.644166571917364E-4 34-35 3.777380817024806E-5 3.777380817024806E-5 0.0 0.17196526169505433 2.644166571917364E-4 36-37 3.777380817024806E-5 3.777380817024806E-5 0.0 0.32319270270464245 2.644166571917364E-4 38-39 3.777380817024806E-5 3.777380817024806E-5 0.0 0.5544722866790288 2.644166571917364E-4 40-41 3.777380817024806E-5 3.777380817024806E-5 0.0 0.8109281137988855 2.644166571917364E-4 42-43 3.777380817024806E-5 3.777380817024806E-5 0.0 1.0657974409755917 2.833035612768605E-4 44-45 3.777380817024806E-5 3.777380817024806E-5 0.0 1.3613113857434849 2.833035612768605E-4 46-47 3.777380817024806E-5 3.777380817024806E-5 0.0 1.7179433521308396 2.927470133194225E-4 48-49 3.777380817024806E-5 3.777380817024806E-5 0.0 2.0998554396361326 3.2107736944710856E-4 50-51 3.777380817024806E-5 3.777380817024806E-5 0.0 2.512836484361454 3.2107736944710856E-4 52-53 3.777380817024806E-5 3.777380817024806E-5 0.0 2.8970527741651324 3.3996427353223256E-4 54-55 5.6660712255372096E-5 3.777380817024806E-5 0.0 3.301997441202235 3.8718153374504263E-4 56-57 5.6660712255372096E-5 3.777380817024806E-5 0.0 3.759362710527598 3.966249857876047E-4 58-59 5.6660712255372096E-5 3.777380817024806E-5 0.0 4.259922329495641 3.966249857876047E-4 60-61 5.6660712255372096E-5 8.499106838305814E-5 0.0 4.7879718373595805 4.060684378301667E-4 62-63 5.6660712255372096E-5 9.443452042562016E-5 0.0 5.297087223878185 4.155118898727287E-4 64-65 5.6660712255372096E-5 9.443452042562016E-5 0.0 5.801773631388826 4.155118898727287E-4 66-67 5.6660712255372096E-5 9.443452042562016E-5 0.0 6.36499999811131 4.2495534191529076E-4 68-69 5.6660712255372096E-5 1.1332142451074419E-4 0.0 6.980864166519034 4.3439879395785276E-4 70-71 5.6660712255372096E-5 1.1332142451074419E-4 0.0 7.632585122332367 4.5328569804297677E-4 72-73 5.6660712255372096E-5 1.322083285958682E-4 0.0 8.272936161886456 4.7217260212810083E-4 74-75 5.6660712255372096E-5 1.322083285958682E-4 0.0 8.881311672824427 4.7217260212810083E-4 76-77 5.6660712255372096E-5 1.322083285958682E-4 0.0 9.520321742188472 4.7217260212810083E-4 78-79 5.6660712255372096E-5 1.322083285958682E-4 0.0 10.222206315251892 4.7217260212810083E-4 80-81 5.6660712255372096E-5 1.322083285958682E-4 0.0 10.960731482240455 4.8161605417066283E-4 82-83 5.6660712255372096E-5 1.322083285958682E-4 9.443452042562015E-6 11.689633771597647 5.099464102983488E-4 84-85 7.554761634049612E-5 1.322083285958682E-4 1.888690408512403E-5 12.382140996782805 5.099464102983488E-4 86-87 7.554761634049612E-5 1.322083285958682E-4 1.888690408512403E-5 12.997023046178104 5.099464102983488E-4 88-89 7.554761634049612E-5 1.322083285958682E-4 1.888690408512403E-5 13.61316107469506 5.288333143834729E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6540 0.0 50.708782 1 GTATCAA 12055 0.0 43.15785 1 TCAACGC 15220 0.0 33.50385 4 ATCAACG 15390 0.0 33.286274 3 CAACGCA 15475 0.0 32.952698 5 TATCAAC 15895 0.0 32.465496 2 AACGCAG 16295 0.0 31.468557 6 GTACATG 23095 0.0 30.796803 1 TACATGG 23425 0.0 30.162426 2 ACATGGG 24165 0.0 28.187574 3 ACGCAGA 19310 0.0 26.141243 7 CGCAGAG 19670 0.0 25.494907 8 CATGGGA 12355 0.0 24.862825 4 GAGTACA 16565 0.0 24.223083 1 AGTACAT 16840 0.0 22.263435 2 CATGGGG 12450 0.0 22.103783 4 GCAGAGT 22210 0.0 21.414265 9 GTATAGG 1340 0.0 21.062906 1 AGAGTAC 19515 0.0 19.103764 10-11 CAGAGTA 20565 0.0 18.745995 10-11 >>END_MODULE