FastQCFastQC Report
Fri 27 May 2016
SRR939838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294674
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103630.19572498703414035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85430.1613508215992146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.10508673432963012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71550.051.8723221
GTATCAA123050.041.270591
TCAACGC160250.031.258684
ATCAACG161400.031.0653463
TATCAAC165250.030.4630242
CAACGCA164350.030.4500145
GTACATG237350.029.394541
TACATGG238150.029.2863882
AACGCAG172750.028.9693786
ACATGGG243650.027.3925463
GAGTACA170650.024.6136231
ACGCAGA208850.023.7575687
CATGGGA127500.023.5131934
CGCAGAG214800.023.0111438
AGTACAT174350.022.5868282
CATGGGG123500.022.0854194
GCAGAGT239450.020.1668329
GTATAGG13450.019.760791
ATGGGAG57050.018.5418875
GTGGTAT40100.018.4637031
Produced by FastQC (version 0.11.5)