FastQCFastQC Report
Fri 27 May 2016
SRR939837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329210
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126030.3785582765881395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89130.26772117108863663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57280.17205282934990584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG220350.032.08012
GTACATG213950.031.908841
GTATCAA186200.031.1098881
ACATGGG228000.029.964693
GGTATCA123300.027.8572371
CATGGGA158100.026.3478224
ATCAACG228200.026.0921693
TCAACGC229150.026.0875194
TATCAAC229350.026.0222232
CATGGGG71700.025.8725784
CAACGCA231500.025.7616065
AACGCAG237900.025.1682826
ATGGGAT61650.023.857085
ATGGGAG58450.023.2962515
TACCTGG23150.022.3376792
ACGCAGA269800.021.8759577
AGTACAT151850.021.8698462
CGCAGAG271550.021.6641148
GAGTACT115800.021.46542512-13
GTCGGGA9450.020.5832792
Produced by FastQC (version 0.11.5)