##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939837_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3329210 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.871503449767363 31.0 30.0 33.0 16.0 34.0 2 29.647778301759278 31.0 30.0 33.0 25.0 34.0 3 29.913266210302144 31.0 30.0 33.0 25.0 34.0 4 33.68733603467489 35.0 33.0 37.0 28.0 37.0 5 33.66356943539158 35.0 33.0 37.0 28.0 37.0 6 33.70601824456853 35.0 33.0 37.0 28.0 37.0 7 33.62347103366865 35.0 33.0 37.0 28.0 37.0 8 33.49329300344526 35.0 33.0 37.0 28.0 37.0 9 34.731503569915986 37.0 34.0 39.0 27.0 39.0 10-11 34.56752893329048 37.0 33.5 39.0 27.0 39.0 12-13 34.44866049302988 37.0 33.0 39.0 27.0 39.0 14-15 35.077140372640955 38.0 33.0 40.0 26.5 41.0 16-17 34.92413440425807 38.0 33.0 40.0 26.0 41.0 18-19 34.80006232709862 38.0 33.0 40.0 25.0 41.0 20-21 35.006171584249714 38.0 34.0 40.0 25.5 41.0 22-23 34.91019310887568 38.0 33.5 40.0 25.0 41.0 24-25 34.69211930157605 38.0 33.0 40.0 24.5 41.0 26-27 34.57960912648947 38.0 33.0 40.0 24.5 41.0 28-29 34.623079048783346 38.0 33.0 40.0 24.0 41.0 30-31 34.399640605428914 38.0 33.0 40.0 24.0 41.0 32-33 34.04796378119734 38.0 33.0 40.0 23.5 41.0 34-35 33.859401930187644 38.0 33.0 40.0 22.5 41.0 36-37 33.63898988048216 38.0 32.0 40.0 20.5 41.0 38-39 33.40930686258902 37.0 31.5 40.0 18.5 41.0 40-41 33.1680925805221 37.0 31.0 40.0 18.0 41.0 42-43 32.76390600172414 37.0 31.0 40.0 15.0 41.0 44-45 32.39040478071374 37.0 30.5 40.0 13.5 41.0 46-47 31.969427882290393 37.0 30.0 39.5 9.0 41.0 48-49 31.589946113342204 36.0 30.0 39.0 8.5 40.5 50-51 30.960157214474307 35.5 29.0 38.5 8.0 40.0 52-53 31.118321313464754 36.0 29.5 39.0 7.0 40.0 54-55 31.22004679788899 36.0 29.5 39.0 4.0 40.0 56-57 30.929865193244044 36.0 29.0 39.0 2.0 40.0 58-59 30.543157085314533 35.0 28.0 39.0 2.0 40.0 60-61 30.139092307183986 35.0 28.0 39.0 2.0 40.0 62-63 29.704311833738334 34.5 27.0 38.0 2.0 40.0 64-65 29.16289885588473 34.0 26.0 38.0 2.0 40.0 66-67 28.572969413164085 34.0 26.0 37.5 2.0 39.5 68-69 28.02389455756771 33.5 25.5 37.0 2.0 39.0 70-71 27.450463923873833 33.0 24.0 36.0 2.0 39.0 72-73 26.793882933188353 32.0 22.5 36.0 2.0 38.5 74-75 26.42888628233124 32.0 22.0 35.0 2.0 38.0 76-77 25.852311359151273 32.0 20.0 35.0 2.0 37.5 78-79 25.169422775973878 31.0 18.0 35.0 2.0 37.0 80-81 24.418959903400506 31.0 12.5 34.0 2.0 36.0 82-83 23.65543402188507 30.5 7.0 34.0 2.0 35.5 84-85 22.862895101240234 29.5 2.0 34.0 2.0 35.0 86-87 21.909546408907822 29.0 2.0 34.0 2.0 35.0 88-89 20.879352008434434 28.0 2.0 34.0 2.0 35.0 90-91 19.431937006076517 26.0 2.0 32.5 2.0 34.5 92-93 17.835513380051125 22.0 2.0 32.0 2.0 34.0 94-95 17.328459604530806 20.0 2.0 32.0 2.0 34.0 96-97 17.012252606474206 19.5 2.0 32.0 2.0 34.0 98-99 16.1110930220683 9.5 2.0 31.5 2.0 34.0 100-101 14.468430498526677 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.792260300427582 1101 2 1.0840673113078765 1101 3 1.0277811127694108 1101 4 0.9774023716133158 1101 5 1.0094115563018207 1101 6 1.0418847888690124 1101 7 0.8739995252923123 1101 8 1.1988174610466302 1101 9 1.406881420014713 1101 10-11 1.3090533799823874 1101 12-13 1.2681644392851865 1101 14-15 1.3502786379108613 1101 16-17 1.3097131904548647 1101 18-19 1.2040748580991902 1101 20-21 0.9218500946685282 1101 22-23 1.3794228292598518 1101 24-25 1.6299718703031232 1101 26-27 1.5097196991968147 1101 28-29 1.314160780177879 1101 30-31 1.3419277825091953 1101 32-33 1.4512949828127617 1101 34-35 1.4507898893934978 1101 36-37 1.3729166760580895 1101 38-39 1.2625954837262086 1101 40-41 1.2621518738953696 1101 42-43 1.2538479246615495 1101 44-45 1.3281178420573596 1101 46-47 1.0015169625970728 1101 48-49 1.0519133428636849 1101 50-51 1.0820753160870247 1101 52-53 0.7985595198596727 1101 54-55 0.9305865782313099 1101 56-57 0.8462607237239617 1101 58-59 0.8596852091252423 1101 60-61 0.9087965692365749 1101 62-63 0.577789007553644 1101 64-65 0.6977503319517346 1101 66-67 0.9045257543469347 1101 68-69 0.7245774928019024 1101 70-71 0.5930796843616797 1101 72-73 0.5795022157402094 1101 74-75 0.5749927060833926 1101 76-77 0.29104989174742 1101 78-79 0.11367050635494635 1101 80-81 -0.04676783442428345 1101 82-83 0.03992547679541403 1101 84-85 0.027453318826154316 1101 86-87 -0.16460260466586618 1101 88-89 -4.119487843435277 1101 90-91 -7.63831854089781 1101 92-93 -10.221865822442172 1101 94-95 -8.149715812789237 1101 96-97 -4.484926086820735 1101 98-99 -3.0579167319175458 1101 100-101 -2.211087398069962 1102 1 1.9233651602942423 1102 2 1.3237370583032018 1102 3 1.0762071080556197 1102 4 1.1325862703191945 1102 5 1.1146329395114591 1102 6 1.2059502536154554 1102 7 1.189715940569691 1102 8 1.160932353821181 1102 9 1.4681018663363972 1102 10-11 1.5081768146711454 1102 12-13 1.4148311339067376 1102 14-15 1.691878380086962 1102 16-17 1.639965970493158 1102 18-19 1.712336050360996 1102 20-21 1.7813882610218528 1102 22-23 1.6730762520008895 1102 24-25 1.8537464954356295 1102 26-27 1.7374595498518417 1102 28-29 1.674237252758779 1102 30-31 1.8241227181026431 1102 32-33 2.013164032353707 1102 34-35 2.000020985327758 1102 36-37 2.0793110262163736 1102 38-39 1.8456027682696785 1102 40-41 1.8333737039837885 1102 42-43 1.8235234253709578 1102 44-45 1.9293626181132808 1102 46-47 1.9323823888855927 1102 48-49 1.776814225329474 1102 50-51 1.8768989502239002 1102 52-53 1.75902093118793 1102 54-55 1.7497582920024577 1102 56-57 1.784483550903179 1102 58-59 1.6989483473464695 1102 60-61 1.6939551018807393 1102 62-63 1.8211824948045674 1102 64-65 1.5890443724996963 1102 66-67 1.6815686598018225 1102 68-69 1.6821108297494014 1102 70-71 1.5282627174645533 1102 72-73 1.580942441360083 1102 74-75 1.5605634933897576 1102 76-77 1.5156223542501905 1102 78-79 1.4209997826359562 1102 80-81 1.3266295645135706 1102 82-83 1.245012212312961 1102 84-85 1.4067425090021324 1102 86-87 1.3847924017333035 1102 88-89 0.6153723407135274 1102 90-91 -4.964147011329381 1102 92-93 -10.041926950787136 1102 94-95 -7.653010944775724 1102 96-97 -3.790552087019016 1102 98-99 -2.280532078010486 1102 100-101 -1.4296020622534371 1103 1 2.13630483022666 1103 2 1.3904895931215364 1103 3 1.086680262834772 1103 4 0.9962459229874909 1103 5 1.0538868773782966 1103 6 0.9826032551213117 1103 7 1.0222913675748657 1103 8 1.0082128149032954 1103 9 1.0000509942068732 1103 10-11 1.0714643116936955 1103 12-13 1.0018930926642398 1103 14-15 1.1988451012016412 1103 16-17 1.1063538823566006 1103 18-19 1.0058860311220954 1103 20-21 1.0203126606290667 1103 22-23 1.1331550263031502 1103 24-25 1.0776163712548055 1103 26-27 1.1901987009107984 1103 28-29 1.0124723772212363 1103 30-31 1.1419408572294003 1103 32-33 1.265070108520753 1103 34-35 1.2616116589064106 1103 36-37 1.2276554968480298 1103 38-39 1.1708093913372224 1103 40-41 0.9700940789624966 1103 42-43 1.2092888112313531 1103 44-45 1.0762735023086876 1103 46-47 1.078306558440353 1103 48-49 1.0970112696644492 1103 50-51 1.0873786197627133 1103 52-53 1.1448616439569328 1103 54-55 1.1646704411470132 1103 56-57 1.0731576412289634 1103 58-59 1.1025227788149294 1103 60-61 1.2998067132997555 1103 62-63 0.9920629442144033 1103 64-65 1.0219515093510445 1103 66-67 0.9463427435449852 1103 68-69 0.9644625494084345 1103 70-71 0.9952013301057683 1103 72-73 1.1565201003490593 1103 74-75 0.954100444631532 1103 76-77 0.7845078620994563 1103 78-79 0.4951299434622989 1103 80-81 0.45703842675829875 1103 82-83 0.013650023570871639 1103 84-85 -0.045845988239616275 1103 86-87 0.13434536028247024 1103 88-89 -0.7253489110432518 1103 90-91 -6.310219527390638 1103 92-93 -9.680994675106938 1103 94-95 -8.28219489647964 1103 96-97 -4.943767697740409 1103 98-99 -3.3955695005544033 1103 100-101 -2.659847919782429 1104 1 1.9518836790253005 1104 2 1.3094120920317884 1104 3 0.9410511588834964 1104 4 0.7609837812229969 1104 5 0.8165590604645772 1104 6 0.647025314238924 1104 7 0.8342696988612488 1104 8 0.9243129865624198 1104 9 1.0057597661523943 1104 10-11 0.9629049478050007 1104 12-13 0.8367617653950532 1104 14-15 0.9673718944232164 1104 16-17 0.882216572615178 1104 18-19 0.9512551115039969 1104 20-21 1.0875219480821485 1104 22-23 0.9831223214367313 1104 24-25 0.9805320829940456 1104 26-27 1.1153427870541535 1104 28-29 0.946401712840256 1104 30-31 1.0755476589138198 1104 32-33 1.168410991807292 1104 34-35 1.246354358257399 1104 36-37 1.0892615576197784 1104 38-39 1.116686937955329 1104 40-41 1.063916330167352 1104 42-43 1.2095663221292412 1104 44-45 1.0491837996860411 1104 46-47 1.1326179677500434 1104 48-49 1.0906670391822857 1104 50-51 0.9992489868839449 1104 52-53 0.9979706938109736 1104 54-55 0.9055688292477448 1104 56-57 1.0661488756951876 1104 58-59 1.027155250837275 1104 60-61 1.1245920896848887 1104 62-63 1.1762036023112046 1104 64-65 1.1873051766948386 1104 66-67 1.301370760548199 1104 68-69 1.0057437865735572 1104 70-71 0.9441727504595612 1104 72-73 0.9537015919792502 1104 74-75 0.9371088251292932 1104 76-77 0.9714937110318012 1104 78-79 0.6737004608039179 1104 80-81 0.5153566792382129 1104 82-83 0.23303237046479452 1104 84-85 0.15624042981373876 1104 86-87 0.46326519343132233 1104 88-89 0.005924529308220627 1104 90-91 -2.7685332485732026 1104 92-93 -7.513253585650514 1104 94-95 -5.377389459784348 1104 96-97 -2.7549846136781326 1104 98-99 -1.6796862761461693 1104 100-101 -1.1156331853288801 1105 1 1.89579483312437 1105 2 1.205325872460861 1105 3 0.8510963741068664 1105 4 0.7224122515878975 1105 5 0.8203313862163171 1105 6 0.8377177146982504 1105 7 0.8588604779776503 1105 8 0.7627767094266176 1105 9 0.9325343323697268 1105 10-11 0.8728262357736725 1105 12-13 0.7529797342174049 1105 14-15 0.8887900446962718 1105 16-17 0.8403440301398533 1105 18-19 0.8327478103165333 1105 20-21 0.7443057061231215 1105 22-23 0.7367481877056363 1105 24-25 0.8840492892495888 1105 26-27 0.6941106105022286 1105 28-29 0.7936952111824027 1105 30-31 1.1380289476379843 1105 32-33 1.140323658216495 1105 34-35 1.0692036612245772 1105 36-37 1.1300026426028893 1105 38-39 1.091846771806651 1105 40-41 1.0252954698636856 1105 42-43 0.9831224826309466 1105 44-45 0.9894870635952628 1105 46-47 1.0436789142792406 1105 48-49 0.7334934487429763 1105 50-51 0.9339448324546851 1105 52-53 0.8284316236729516 1105 54-55 0.891561197449537 1105 56-57 1.0150585395193126 1105 58-59 0.9383674615155968 1105 60-61 1.018294108257738 1105 62-63 0.9598983542404831 1105 64-65 1.277384717116906 1105 66-67 1.0429813990071715 1105 68-69 0.8550739663890212 1105 70-71 0.7471572849014798 1105 72-73 0.8129964840059714 1105 74-75 0.8166040667700436 1105 76-77 0.753937013070189 1105 78-79 0.4560108475480753 1105 80-81 0.5830888469263655 1105 82-83 0.3961478502877682 1105 84-85 0.3672226875065441 1105 86-87 0.4797524886510587 1105 88-89 0.16279945175014277 1105 90-91 -2.8765771049044027 1105 92-93 -7.566390212632292 1105 94-95 -5.185701126557877 1105 96-97 -2.551562539815171 1105 98-99 -1.5911649801255425 1105 100-101 -1.1568053234335451 1106 1 1.7300398080164783 1106 2 1.07588123117527 1106 3 0.7705995982303442 1106 4 0.8376701276801697 1106 5 0.8492200838667472 1106 6 0.8628888040156468 1106 7 0.7972586590758368 1106 8 -0.07948252005123635 1106 9 -0.32205931243278485 1106 10-11 -0.46012216480426105 1106 12-13 -0.09076692517921003 1106 14-15 0.6366448730825525 1106 16-17 0.7848113102113388 1106 18-19 0.9278963045490158 1106 20-21 0.7976470742729092 1106 22-23 0.8564170049085007 1106 24-25 0.9005559879367411 1106 26-27 0.9172901875574624 1106 28-29 0.7103011867192635 1106 30-31 0.8620205224261497 1106 32-33 0.8044135501137291 1106 34-35 0.9086818699014358 1106 36-37 0.8469371402198433 1106 38-39 0.7537465305100355 1106 40-41 0.7110041921076871 1106 42-43 0.8140247531319105 1106 44-45 0.7836634128292488 1106 46-47 0.871322898907259 1106 48-49 0.7418042816768349 1106 50-51 0.6409855973965506 1106 52-53 0.7486743607389741 1106 54-55 0.6625909890765662 1106 56-57 0.7460673143389478 1106 58-59 0.5918252843599099 1106 60-61 0.6018547847084967 1106 62-63 0.7556238610205241 1106 64-65 0.6654283236306391 1106 66-67 0.7182971049359104 1106 68-69 0.683567640840959 1106 70-71 0.49733944233121363 1106 72-73 0.5463105272806921 1106 74-75 0.5049613202242682 1106 76-77 0.5279932653014612 1106 78-79 0.4016349848740184 1106 80-81 0.23756187703768106 1106 82-83 0.11473731223463801 1106 84-85 0.11941975402816851 1106 86-87 0.25906962393785093 1106 88-89 -0.02248066477682542 1106 90-91 -3.5501517471783046 1106 92-93 -8.371060300525038 1106 94-95 -5.789161798369605 1106 96-97 -2.9571370866192517 1106 98-99 -1.9206502044897107 1106 100-101 -1.4852723991189283 1107 1 1.8794722330289488 1107 2 1.1780581210551766 1107 3 1.0221151682493925 1107 4 0.9808318576748505 1107 5 0.8640528664489509 1107 6 0.84627386443087 1107 7 0.7481960241083243 1107 8 0.7607120173010742 1107 9 0.8222644354872557 1107 10-11 0.8128055712905393 1107 12-13 0.8695587378146712 1107 14-15 0.9279003596686621 1107 16-17 0.7596009283014169 1107 18-19 0.8667537637951028 1107 20-21 0.8016511103245136 1107 22-23 0.8021495206526126 1107 24-25 0.715813468275428 1107 26-27 0.7425161378124159 1107 28-29 0.8184279046777689 1107 30-31 0.9186906794153344 1107 32-33 1.015893907214739 1107 34-35 0.9033746964098057 1107 36-37 0.6993761809419468 1107 38-39 0.7574816778066662 1107 40-41 0.7566536766782761 1107 42-43 0.7695761236484842 1107 44-45 0.7190223468830865 1107 46-47 0.6778751214977561 1107 48-49 0.7013452498604948 1107 50-51 0.8000562508987592 1107 52-53 0.7663866593967477 1107 54-55 0.7077564738882245 1107 56-57 0.7107049485531469 1107 58-59 0.7455616871682373 1107 60-61 0.5516619799623257 1107 62-63 0.5659337882678024 1107 64-65 0.6973326754015794 1107 66-67 0.7035066749482688 1107 68-69 0.9118275351101381 1107 70-71 0.8295725707650412 1107 72-73 0.7373316935338785 1107 74-75 0.6095447749237337 1107 76-77 0.6163955597072359 1107 78-79 0.4636906592846799 1107 80-81 0.3510328479332472 1107 82-83 0.10151095686791223 1107 84-85 0.4911229477151231 1107 86-87 0.7007532818628057 1107 88-89 0.37144224010895144 1107 90-91 -2.6482658849096126 1107 92-93 -4.196882325883026 1107 94-95 -2.338682988059009 1107 96-97 -0.7022701373787541 1107 98-99 -0.35461752937930946 1107 100-101 -0.01959871665290258 1108 1 1.6661179032816342 1108 2 1.0078277267780251 1108 3 -0.6044644926191616 1108 4 -0.2109860470292233 1108 5 0.45347281039157394 1108 6 0.6187578791637023 1108 7 0.5517110358615795 1108 8 0.5270029734612223 1108 9 0.6836357326503659 1108 10-11 0.5570143884189207 1108 12-13 0.5978043364193013 1108 14-15 0.43334986887547444 1108 16-17 0.5507724743852975 1108 18-19 0.617029733302914 1108 20-21 0.4898955281820676 1108 22-23 0.4775620291015841 1108 24-25 0.5310996849810721 1108 26-27 0.6399968894262074 1108 28-29 0.38651909851346744 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90-91 2.3998654457669772 2311 92-93 3.430467912518278 2311 94-95 2.9607547053532848 2311 96-97 2.0095704283121236 2311 98-99 1.83658098059791 2311 100-101 1.4365135310021717 2312 1 1.0962462353895148 2312 2 0.3960627600839288 2312 3 -0.26371515182638916 2312 4 -0.8461958603851087 2312 5 -0.3052872428532325 2312 6 -0.36146767606602026 2312 7 -0.13429971549930997 2312 8 0.1317243819195113 2312 9 0.1154416697644578 2312 10-11 -0.01731551025292788 2312 12-13 -0.003975419409542269 2312 14-15 0.11345114952722923 2312 16-17 -0.263788870135798 2312 18-19 -0.5005658307257903 2312 20-21 0.21689227062121574 2312 22-23 0.19585234992845102 2312 24-25 0.3443018587795308 2312 26-27 0.35765584623307944 2312 28-29 0.17188749184096253 2312 30-31 0.2613462086598659 2312 32-33 0.29988589583961556 2312 34-35 0.16791157739209694 2312 36-37 0.14674286946279125 2312 38-39 0.17964697751192915 2312 40-41 0.11053146450350937 2312 42-43 0.327180381577854 2312 44-45 0.006527385268647379 2312 46-47 0.21224658705318689 2312 48-49 0.18541429499700257 2312 50-51 -0.06595346111474498 2312 52-53 -0.09970616887435213 2312 54-55 0.05090616564262973 2312 56-57 0.1784396161252566 2312 58-59 0.27383051939263936 2312 60-61 0.17749839106017973 2312 62-63 0.14767299791273203 2312 64-65 0.20641521813353947 2312 66-67 0.4328257979818311 2312 68-69 0.28241204256812225 2312 70-71 0.44270149909310064 2312 72-73 0.35692883380292173 2312 74-75 0.17654316381320712 2312 76-77 0.5420224599751542 2312 78-79 0.3905917091063493 2312 80-81 0.48532655326460983 2312 82-83 0.45772542183339127 2312 84-85 0.8334814712169667 2312 86-87 1.2041256673267675 2312 88-89 1.4752262582522242 2312 90-91 2.39064839626964 2312 92-93 3.3220403060171186 2312 94-95 3.1978241274510886 2312 96-97 2.339844393383842 2312 98-99 2.217749755342348 2312 100-101 2.073988000071177 2313 1 -7.497091302386515 2313 2 -2.838660258324147 2313 3 -1.7623559995908735 2313 4 -0.7269204043692596 2313 5 -0.663706486739585 2313 6 -0.277697764798269 2313 7 -0.25445758122768325 2313 8 -0.11468922070746146 2313 9 -0.0346176904935831 2313 10-11 -0.3035251949865838 2313 12-13 -0.4472116639342687 2313 14-15 -0.4269427139244115 2313 16-17 -0.7937691071233388 2313 18-19 -0.8886350801935308 2313 20-21 -0.7165425909432201 2313 22-23 -1.3839786215914103 2313 24-25 -0.5256415091505744 2313 26-27 -0.5299971619541068 2313 28-29 -0.7035062204484106 2313 30-31 -0.27165963550579875 2313 32-33 -0.6368222101204779 2313 34-35 -0.46618432111058183 2313 36-37 -0.3143170047469539 2313 38-39 -0.3151464272785205 2313 40-41 -0.2890963202251484 2313 42-43 -0.28359201543704415 2313 44-45 -0.4372524256738757 2313 46-47 -1.4722777329201122 2313 48-49 -0.9511134264179226 2313 50-51 -0.7517048843655942 2313 52-53 -1.0234243492179083 2313 54-55 -0.618673947740696 2313 56-57 -0.4785534772127882 2313 58-59 -0.5177263123858573 2313 60-61 -0.2444444321043875 2313 62-63 -0.3518191550216194 2313 64-65 -0.4230262634659425 2313 66-67 -0.3202454849090408 2313 68-69 -0.3824076586422258 2313 70-71 -0.3591980291643573 2313 72-73 -0.36567925593629624 2313 74-75 -0.24395202631984958 2313 76-77 -0.029372203764392424 2313 78-79 -0.1710752632763075 2313 80-81 -0.0623933747472698 2313 82-83 0.16247289073757187 2313 84-85 0.10940710752288751 2313 86-87 0.6624081893063369 2313 88-89 1.2618067992503335 2313 90-91 1.9934556684535707 2313 92-93 3.0145899613671574 2313 94-95 2.5644664167803093 2313 96-97 1.7677889761353 2313 98-99 1.4063332181325965 2313 100-101 1.4102181135126415 2314 1 0.5041941705255013 2314 2 -0.10262738094846924 2314 3 -1.4403347405796865 2314 4 -0.43756975698388345 2314 5 -0.12637706344396094 2314 6 -3.538145242364152 2314 7 -2.9403326427767276 2314 8 -2.603924467270314 2314 9 -3.5263773937078717 2314 10-11 -2.602633839673693 2314 12-13 -1.386267923544949 2314 14-15 -1.2101082278924125 2314 16-17 -2.208880350838932 2314 18-19 -3.361989909130834 2314 20-21 -3.023243776002431 2314 22-23 -4.249859468459711 2314 24-25 -3.162371731390646 2314 26-27 -1.9607552133840827 2314 28-29 -2.006453714019962 2314 30-31 -1.9503641605101336 2314 32-33 -2.2593273275671173 2314 34-35 -1.7203654365247942 2314 36-37 -2.2061491408567697 2314 38-39 -3.4467000910618424 2314 40-41 -2.870635018714566 2314 42-43 -1.9063036786755845 2314 44-45 -2.345024491370424 2314 46-47 -1.9163232847429583 2314 48-49 -2.4647487953222367 2314 50-51 -3.3220327079680665 2314 52-53 -2.039282140657729 2314 54-55 -2.575668734842111 2314 56-57 -1.5582900109218762 2314 58-59 -1.0535344702680298 2314 60-61 -1.5668846393165978 2314 62-63 -1.2755347152023262 2314 64-65 -1.7683116793396003 2314 66-67 -1.137228234297627 2314 68-69 -1.098998967742407 2314 70-71 -0.9079576776683815 2314 72-73 -1.9759167974200658 2314 74-75 -2.1655468362948085 2314 76-77 -1.21435211229349 2314 78-79 -2.1606908989455356 2314 80-81 -1.2749737013442548 2314 82-83 -0.9999615151497174 2314 84-85 -1.9900519302050768 2314 86-87 -0.8610552016706592 2314 88-89 0.20064581837680961 2314 90-91 1.3833945530160818 2314 92-93 2.4764704040197465 2314 94-95 2.11172637113798 2314 96-97 1.1774732455379429 2314 98-99 1.1488542452071915 2314 100-101 1.209604274551717 2315 1 -2.814149487965963 2315 2 -1.9127094911065967 2315 3 -2.750667591951899 2315 4 -3.945096412600261 2315 5 -4.145045198358947 2315 6 -3.281165202594483 2315 7 -6.039275010571551 2315 8 -3.1114495857758016 2315 9 -2.473016512273148 2315 10-11 -3.9656100875445084 2315 12-13 -3.0946540086026673 2315 14-15 -2.042830713357681 2315 16-17 -2.5751002101118203 2315 18-19 -2.201503312084533 2315 20-21 -1.4557359407718025 2315 22-23 -0.8247621904755889 2315 24-25 -2.2859117546455963 2315 26-27 -3.9106223801036606 2315 28-29 -3.2667730503446606 2315 30-31 -3.1574929272868317 2315 32-33 -4.084621263518628 2315 34-35 -3.79606764834282 2315 36-37 -2.911154581076488 2315 38-39 -1.860969767791282 2315 40-41 -1.541130013766221 2315 42-43 -2.871433126853546 2315 44-45 -6.369345851147905 2315 46-47 -3.190559661502057 2315 48-49 -2.2170731567698674 2315 50-51 -3.349787243348249 2315 52-53 -3.49288344929246 2315 54-55 -2.0551032286711113 2315 56-57 -1.5634267665250121 2315 58-59 -2.844485160288638 2315 60-61 -3.073955118848314 2315 62-63 -5.024289866733408 2315 64-65 -4.539513338066705 2315 66-67 -3.859314550298574 2315 68-69 -2.279752026035876 2315 70-71 -3.6931459310976926 2315 72-73 -2.73833175387794 2315 74-75 -1.815685833389903 2315 76-77 -3.4879430946172505 2315 78-79 -2.023958736752359 2315 80-81 -1.7538899582363712 2315 82-83 -1.118192780092837 2315 84-85 -2.5487485846755398 2315 86-87 -2.1699280282631044 2315 88-89 -1.5392430853964356 2315 90-91 -0.7080819134703304 2315 92-93 -0.1598829756221285 2315 94-95 -0.23527825702605298 2315 96-97 -0.4487155656320567 2315 98-99 -0.7476706775472213 2315 100-101 -1.1692971338491933 2316 1 -7.2329047820369965 2316 2 -6.2589009320940825 2316 3 -3.6843941038415373 2316 4 -4.131116463840414 2316 5 -2.322877861721519 2316 6 -3.0810267998072405 2316 7 -3.6312752075565093 2316 8 -2.6715090245435142 2316 9 -7.164853274302406 2316 10-11 -7.118776841327829 2316 12-13 -6.459535079334621 2316 14-15 -5.221265881204346 2316 16-17 -5.064260262556658 2316 18-19 -4.411700219244217 2316 20-21 -3.176632596093924 2316 22-23 -3.693309619697555 2316 24-25 -3.4354655668700076 2316 26-27 -4.3191831812036305 2316 28-29 -4.437648038123335 2316 30-31 -6.981367400065331 2316 32-33 -5.180048044345671 2316 34-35 -5.869536862264553 2316 36-37 -4.622775177778902 2316 38-39 -6.657600362523038 2316 40-41 -4.027071856523619 2316 42-43 -4.723216261813022 2316 44-45 -3.2187160005637274 2316 46-47 -4.485451158160249 2316 48-49 -5.605337729706719 2316 50-51 -4.847579624249146 2316 52-53 -6.482753441625604 2316 54-55 -5.381707900150165 2316 56-57 -4.631841824421027 2316 58-59 -3.312510074073632 2316 60-61 -3.5759234044303057 2316 62-63 -4.500781604714216 2316 64-65 -3.4463059264594946 2316 66-67 -4.536513027964158 2316 68-69 -3.5275552948472786 2316 70-71 -2.9159243702597806 2316 72-73 -2.435449533478881 2316 74-75 -2.5545041899177576 2316 76-77 -3.143371084944775 2316 78-79 -5.09337929218097 2316 80-81 -6.088680814508976 2316 82-83 -3.829402684008933 2316 84-85 -2.3270116553614564 2316 86-87 -2.2963593674151426 2316 88-89 -1.5303452763969325 2316 90-91 -1.3780111969928797 2316 92-93 -1.4658428052878882 2316 94-95 -2.1452202788023556 2316 96-97 -3.6290674869612687 2316 98-99 -4.802757168461492 2316 100-101 -4.23992947561938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24728.0 3 8654.0 4 8251.0 5 9392.0 6 10692.0 7 12438.0 8 15446.0 9 18336.0 10 21883.0 11 26555.0 12 31088.0 13 35222.0 14 38684.0 15 43792.0 16 49200.0 17 52375.0 18 47840.0 19 46149.0 20 49526.0 21 54313.0 22 60745.0 23 68133.0 24 76508.0 25 87164.0 26 98059.0 27 110715.0 28 125293.0 29 142551.0 30 160105.0 31 180935.0 32 201867.0 33 224594.0 34 254065.0 35 291352.0 36 314937.0 37 251449.0 38 74884.0 39 1290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.16333886546572 19.25170039382607 12.284251348494864 22.300709392213346 2 15.943048426099878 20.624014536220407 39.447932777799664 23.98500425988005 3 19.911501630209386 25.92966746495448 29.13360559128285 25.02522531355328 4 12.470944851764603 15.830273419171345 37.26446080141678 34.43432092764726 5 13.13869403405887 38.89703394609225 33.5150407879403 14.449231231908573 6 31.530241381810022 35.69024176682257 18.158785790751097 14.620731060616315 7 29.058870078426242 33.36694242975413 20.362087615333813 17.212099876485816 8 27.4059468611649 33.851999956678334 20.12405639999374 18.617996782163026 9 28.31759144257339 15.75278251157775 19.8796732233808 36.04995282246806 10-11 25.334771229756452 26.7774713380951 27.167667969277677 20.72008946287077 12-13 26.427602007671435 24.298230269125984 27.669017292994795 21.605150430207786 14-15 23.229688581387748 25.296160633044813 26.406525153365745 25.0676256322017 16-17 22.07721064359049 28.872542663917915 26.88284836646883 22.167398326022756 18-19 21.9543441972866 27.205330160919374 29.656414089080325 21.183911552713703 20-21 23.046897684317198 26.282583048013986 29.40156806036103 21.268951207307786 22-23 22.46672890284579 26.555685001055746 29.098915341689718 21.878670754408745 24-25 22.09467561796051 26.636030192101174 29.607039645272014 21.6622545446663 26-27 21.950145080096288 26.809549195574412 29.61338220675009 21.626923517579208 28-29 21.641506471366725 27.069421484368473 29.73417363071426 21.554898413550543 30-31 21.724796632927703 27.02278734818972 29.58098254445174 21.67143347443084 32-33 21.49993888380758 26.999854284546153 29.442379473148318 22.05782735849795 34-35 22.1191921004197 26.90052494086212 29.296053039557506 21.68422991916067 36-37 22.183298626237242 26.590337147049603 29.262432310625552 21.963931916087603 38-39 21.85200938709359 26.77603425398133 29.521181165126638 21.850775193798448 40-41 21.848437955549574 26.731184339713604 29.617140050643005 21.803237654093817 42-43 21.6713374070618 26.752837060895217 29.355437825471842 22.220387706571145 44-45 21.68451021920187 26.97438272956615 29.206222646420144 22.134884404811835 46-47 21.841448963025588 27.110031860244206 28.705572780721834 22.34294639600837 48-49 21.76929442886467 27.095680707739454 28.646435644279972 22.488589219115905 50-51 21.777625456843595 27.315870066160723 28.573115077378247 22.333389399617435 52-53 21.804124968791534 27.39127632616786 28.435544012145474 22.36905469289513 54-55 21.322356436055053 27.24901362419812 28.664844244326666 22.763785695420165 56-57 21.56309674797054 27.55328909981145 28.35263876918908 22.53097538302893 58-59 21.611041093234306 26.95137614734143 28.521494417671722 22.916088341752538 60-61 21.466940671774037 27.111847209294098 28.739970306916323 22.68124181201554 62-63 21.56873160985451 26.955158480485846 28.65983176134724 22.8162781483124 64-65 21.24283817515055 26.97815196665106 28.633409524480264 23.14560033371813 66-67 21.322253825261164 27.198593018459107 28.324759175863512 23.154393980416216 68-69 21.454472066440477 27.005350490862988 28.43519813912721 23.104979303569323 70-71 21.32051717170501 27.062640206555628 28.338562192824885 23.278280428914478 72-73 21.453690413065022 26.917427645645915 28.24042750322112 23.38845443806795 74-75 21.25426270455336 27.139075958572157 28.169201329135745 23.43746000773874 76-77 21.41341001349512 26.917347872688683 28.272446670776542 23.396795443039654 78-79 21.56348510229818 26.873443881872422 28.210054772346556 23.353016243482845 80-81 21.426358704532582 27.211424050027656 28.065845331902047 23.296371913537715 82-83 21.582562196224988 27.01005422431935 27.895931956204894 23.511451623250768 84-85 21.414239322871616 26.744150157537018 27.998875628187044 23.842734891404323 86-87 21.447839045750932 26.54580362998638 28.28158622346874 23.724771100793944 88-89 21.710883221408604 26.41878353996534 28.096608049034582 23.773725189591474 90-91 21.41545016055829 26.601630804022342 28.3739415280612 23.608977507358166 92-93 21.874086551730233 26.26533025833129 28.343675739252035 23.516907450686446 94-95 21.48919064120735 26.07332927949413 28.452676701802453 23.98480337749607 96-97 22.03104800380407 25.968839934552385 27.856340587868754 24.143771473774787 98-99 22.072125824234437 25.97158865932969 27.55953381824625 24.39675169818962 100-101 22.2741215891906 25.937771495266894 27.85722089815626 23.930886017386246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4755.0 1 2901.5 2 1083.0 3 1138.0 4 1048.0 5 1014.5 6 1428.0 7 2315.0 8 3427.5 9 3830.0 10 3462.5 11 3221.0 12 3202.0 13 3283.0 14 3480.0 15 3839.0 16 4168.0 17 4470.5 18 4902.0 19 5416.5 20 6166.5 21 7236.5 22 8357.5 23 9844.0 24 11781.0 25 14208.5 26 17965.5 27 22482.5 28 26385.0 29 30771.5 30 36157.0 31 42317.0 32 50139.0 33 57446.5 34 64409.5 35 71663.0 36 80024.5 37 88464.5 38 93392.0 39 96081.5 40 99348.5 41 104794.0 42 110372.0 43 115767.0 44 121187.0 45 125736.0 46 128577.5 47 131734.0 48 139535.5 49 140181.0 50 131943.5 51 125236.5 52 119443.5 53 113938.5 54 107285.5 55 98150.0 56 87576.0 57 77391.0 58 68603.5 59 59726.5 60 50757.5 61 42477.5 62 34069.0 63 25640.5 64 18681.5 65 13966.5 66 10326.5 67 7641.0 68 5634.0 69 4061.0 70 3094.0 71 2473.0 72 2030.5 73 1706.0 74 1354.0 75 1075.5 76 785.5 77 523.5 78 352.0 79 242.5 80 141.0 81 98.5 82 87.0 83 51.5 84 27.5 85 21.5 86 14.0 87 7.5 88 3.5 89 3.5 90 4.5 91 4.0 92 5.0 93 4.0 94 3.5 95 3.5 96 2.5 97 2.5 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13192318898477418 2 0.1201185866917377 3 0.1254652004529603 4 0.1351071275167382 5 0.12270178210446323 6 0.13946251513121732 7 0.17136197476278156 8 0.15733462292856262 9 0.1680278504510079 10-11 0.16138963898342248 12-13 0.15200302774532096 14-15 0.15653863829557163 16-17 0.15111693164444417 18-19 0.1669465128363786 20-21 0.17244331237741087 22-23 0.20784210067853934 24-25 0.2306553206316213 26-27 0.2437064648970777 28-29 0.2380594795762358 30-31 0.229183499989487 32-33 0.23032491191604015 34-35 0.21963168439359487 36-37 0.228372496778515 38-39 0.21632759723778316 40-41 0.22015733462292858 42-43 0.21356417888928605 44-45 0.20609994563274772 46-47 0.2210284121458244 48-49 0.26030199356604117 50-51 0.26238957590539497 52-53 0.26423686099705335 54-55 0.2619540371439471 56-57 0.23496565251215754 58-59 0.23537115411764353 60-61 0.20688091168775774 62-63 0.1985005451743807 64-65 0.19961191994497196 66-67 0.20787213783450129 68-69 0.20719630182535798 70-71 0.2133389002195716 72-73 0.24507315549334527 74-75 0.24795672246569006 76-77 0.2628551518228048 78-79 0.25974630618074557 80-81 0.2741341038865076 82-83 0.27372860228102164 84-85 0.24764133232808982 86-87 0.24978898897936747 88-89 0.2538590236122083 90-91 0.43631972750292114 92-93 0.5795218685513981 94-95 0.3414473703971813 96-97 0.28861201306015544 98-99 0.32067667704951025 100-101 0.34114699883756205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3329210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.0766515686678 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74929989134968 38.179439455523244 2 12.662950313090443 12.935622019461468 3 4.98618182743265 7.640324155734021 4 2.4806830812509237 5.068199415733707 5 1.3793761377700788 3.5226957185508523 6 0.9033633110591404 2.7684463847331466 7 0.586884438901921 2.0983264397806565 8 0.4078294314681587 1.666444941643762 9 0.2953547022988121 1.3577156296635629 >10 1.3802408685459167 12.494326929534818 >50 0.10241098038000476 3.6335098490927225 >100 0.06076285724917742 5.741607388223847 >500 0.0033301137171636327 1.1548873580866839 >1k 0.001150402919634574 0.8959159903842908 >5k 1.2109504417206042E-4 0.4527823961068831 >10k+ 6.054752208603021E-5 0.38975592774640033 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12603 0.3785582765881395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8913 0.26772117108863663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5728 0.17205282934990584 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.00743119238498E-5 0.0 0.0 0.0 0.0 3 6.00743119238498E-5 0.0 0.0 0.0 0.0 4 6.00743119238498E-5 0.0 0.0 0.0 0.0 5 1.501857798096245E-4 0.0 0.0 0.0 0.0 6 1.501857798096245E-4 0.0 0.0 0.0 0.0 7 1.802229357715494E-4 0.0 0.0 0.0 0.0 8 1.802229357715494E-4 0.0 0.0 0.0 0.0 9 1.802229357715494E-4 0.0 0.0 3.00371559619249E-5 0.0 10-11 2.1026009173347431E-4 0.0 0.0 6.00743119238498E-5 0.0 12-13 2.5531582567636164E-4 0.0 0.0 2.402972476953992E-4 0.0 14-15 3.4542729356213634E-4 0.0 0.0 2.402972476953992E-4 0.0 16-17 3.604458715430988E-4 0.0 0.0 4.0550160548598617E-4 1.501857798096245E-5 18-19 4.2052018346694863E-4 0.0 0.0 5.556873852956107E-4 3.00371559619249E-5 20-21 4.505573394288735E-4 0.0 0.0 8.560589449148596E-4 3.00371559619249E-5 22-23 4.505573394288735E-4 0.0 0.0 0.0015168763760772075 3.00371559619249E-5 24-25 6.608174311623479E-4 0.0 0.0 0.0027784369264780536 3.00371559619249E-5 26-27 6.608174311623479E-4 0.0 3.00371559619249E-5 0.005241483715355895 3.00371559619249E-5 28-29 7.509288990481225E-4 0.0 3.00371559619249E-5 0.012976051375551557 3.00371559619249E-5 30-31 8.110032109719723E-4 0.0 3.00371559619249E-5 0.04266778004391432 3.00371559619249E-5 32-33 8.260217889529348E-4 0.0 3.00371559619249E-5 0.11070193829767422 3.00371559619249E-5 34-35 9.161332568387096E-4 0.0 3.00371559619249E-5 0.22269547430171122 3.00371559619249E-5 36-37 0.0010513004586673715 0.0 3.00371559619249E-5 0.409977141724313 3.00371559619249E-5 38-39 0.0011414119265531462 0.0 6.00743119238498E-5 0.6928520579957407 3.00371559619249E-5 40-41 0.0011564305045341087 0.0 6.00743119238498E-5 1.0096689605041436 3.00371559619249E-5 42-43 0.0012615605504008459 0.0 6.00743119238498E-5 1.3113020806737934 3.00371559619249E-5 44-45 0.0013516720182866206 0.0 6.00743119238498E-5 1.665500223776812 3.00371559619249E-5 46-47 0.0015168763760772075 0.0 6.00743119238498E-5 2.0770242790331643 3.00371559619249E-5 48-49 0.0016220064219439447 0.0 6.00743119238498E-5 2.5276116556179993 3.00371559619249E-5 50-51 0.0016520435779058696 0.0 6.00743119238498E-5 3.0184488211918143 3.00371559619249E-5 52-53 0.0016820807338677945 0.0 6.00743119238498E-5 3.472670693648043 3.00371559619249E-5 54-55 0.0016820807338677945 0.0 6.00743119238498E-5 3.9352729326176483 3.00371559619249E-5 56-57 0.001802229357715494 0.0 6.00743119238498E-5 4.466960630299681 3.00371559619249E-5 58-59 0.001802229357715494 0.0 6.00743119238498E-5 5.051093202291234 3.00371559619249E-5 60-61 0.0018322665136774189 0.0 6.00743119238498E-5 5.693452801114979 3.00371559619249E-5 62-63 0.0018623036696393438 0.0 6.00743119238498E-5 6.298701493747766 3.00371559619249E-5 64-65 0.0018773222476203063 0.0 6.00743119238498E-5 6.876691467345106 3.00371559619249E-5 66-67 0.0019524151375251186 0.0 6.00743119238498E-5 7.509138804701416 3.00371559619249E-5 68-69 0.0019524151375251186 0.0 6.00743119238498E-5 8.212518885861812 3.00371559619249E-5 70-71 0.0019974708714680057 0.0 6.00743119238498E-5 8.97274428468015 3.00371559619249E-5 72-73 0.002072563761372818 0.0 6.00743119238498E-5 9.705020109875917 3.00371559619249E-5 74-75 0.002102600917334743 0.0 6.00743119238498E-5 10.39012258163348 3.00371559619249E-5 76-77 0.002102600917334743 0.0 6.00743119238498E-5 11.09590563527083 3.00371559619249E-5 78-79 0.002102600917334743 0.0 6.00743119238498E-5 11.874709015051618 3.00371559619249E-5 80-81 0.0023428981650301423 3.00371559619249E-5 6.00743119238498E-5 12.686718470748314 3.00371559619249E-5 82-83 0.0024179910549349546 3.00371559619249E-5 6.00743119238498E-5 13.435905214750647 3.00371559619249E-5 84-85 0.002493083944839767 3.00371559619249E-5 6.00743119238498E-5 14.127270433526272 3.00371559619249E-5 86-87 0.0025531582567636167 3.00371559619249E-5 6.00743119238498E-5 14.824853343586016 3.00371559619249E-5 88-89 0.0025531582567636167 3.00371559619249E-5 6.00743119238498E-5 15.557820023368908 3.00371559619249E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 22035 0.0 32.0801 2 GTACATG 21395 0.0 31.90884 1 GTATCAA 18620 0.0 31.109888 1 ACATGGG 22800 0.0 29.96469 3 GGTATCA 12330 0.0 27.857237 1 CATGGGA 15810 0.0 26.347822 4 ATCAACG 22820 0.0 26.092169 3 TCAACGC 22915 0.0 26.087519 4 TATCAAC 22935 0.0 26.022223 2 CATGGGG 7170 0.0 25.872578 4 CAACGCA 23150 0.0 25.761606 5 AACGCAG 23790 0.0 25.168282 6 ATGGGAT 6165 0.0 23.85708 5 ATGGGAG 5845 0.0 23.296251 5 TACCTGG 2315 0.0 22.337679 2 ACGCAGA 26980 0.0 21.875957 7 AGTACAT 15185 0.0 21.869846 2 CGCAGAG 27155 0.0 21.664114 8 GAGTACT 11580 0.0 21.465425 12-13 GTCGGGA 945 0.0 20.583279 2 >>END_MODULE