FastQCFastQC Report
Fri 27 May 2016
SRR939837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329210
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141650.42547631420066623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111110.33374283989294756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75550.2269307132923426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132650.032.5718881
GTATCAA199900.030.863821
GTACATG227950.029.176571
TACATGG230950.028.5060672
ACATGGG232400.027.1297363
ATCAACG240600.025.3231163
TCAACGC241500.025.226854
TATCAAC247250.024.738512
CAACGCA246800.024.6665
AACGCAG250950.024.298036
CATGGGA159100.024.1845264
CATGGGG70450.022.221574
ATGGGAG56950.021.9416755
GAGTACT130700.021.10969212-13
ATGGGAT62350.020.9488875
ACGCAGA290250.020.894427
CGCAGAG289450.020.887058
AGTACAT165200.020.5252632
GTCGGGA9750.020.3148922
TACCTGG25650.020.2243792
Produced by FastQC (version 0.11.5)