##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939837_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3329210 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43499689115436 31.0 31.0 34.0 30.0 34.0 2 31.635067778842426 31.0 31.0 34.0 30.0 34.0 3 31.697322788289114 31.0 31.0 34.0 30.0 34.0 4 35.50037005776145 37.0 35.0 37.0 33.0 37.0 5 35.24567179601166 37.0 35.0 37.0 33.0 37.0 6 35.279699087771576 37.0 35.0 37.0 32.0 37.0 7 35.194953457426834 37.0 35.0 37.0 32.0 37.0 8 35.13727520943407 37.0 35.0 37.0 32.0 37.0 9 36.72921473863169 39.0 35.0 39.0 32.0 39.0 10-11 36.5922505639476 39.0 35.0 39.0 32.0 39.0 12-13 36.5332448238471 38.0 35.0 39.0 32.0 39.0 14-15 37.5618472550545 39.0 36.0 41.0 32.0 41.0 16-17 37.504878785057116 39.0 36.0 41.0 32.0 41.0 18-19 37.50719975609829 39.0 36.0 41.0 32.0 41.0 20-21 37.44535955977544 39.0 36.0 41.0 32.0 41.0 22-23 37.30035158491054 39.0 36.0 40.5 31.0 41.0 24-25 37.14303753743381 39.0 36.0 40.5 31.0 41.0 26-27 36.85717572637353 39.0 36.0 40.0 30.5 41.0 28-29 36.63307105889986 38.5 35.0 40.0 30.0 41.0 30-31 36.378186266411554 38.0 35.0 40.0 30.0 41.0 32-33 36.31963694089589 38.0 35.0 40.0 30.0 41.0 34-35 36.35610039619009 38.0 35.0 40.0 30.0 41.0 36-37 36.308189780758795 38.0 35.0 40.0 30.0 41.0 38-39 36.166174407742375 38.0 35.0 40.0 30.0 41.0 40-41 35.98397893193881 38.0 35.0 40.0 29.5 41.0 42-43 35.766242291714846 38.0 35.0 40.0 28.5 41.0 44-45 35.486915965048766 38.0 34.0 40.0 27.5 41.0 46-47 35.14506639713325 38.0 34.0 40.0 26.5 41.0 48-49 34.81749994743498 38.0 34.0 40.0 25.5 41.0 50-51 34.55574520682084 38.0 33.0 40.0 24.0 41.0 52-53 34.275543447244246 38.0 33.0 40.0 24.0 41.0 54-55 33.97340795564113 38.0 33.0 40.0 23.5 41.0 56-57 32.64389314582138 36.5 31.5 39.5 15.5 41.0 58-59 29.23317018151453 34.5 23.5 39.0 7.0 40.0 60-61 29.22268075609529 34.0 24.0 38.0 7.5 40.0 62-63 31.193733197965884 35.0 29.0 38.5 8.0 40.0 64-65 30.80326804256866 35.0 29.5 38.5 2.0 40.0 66-67 25.35081340618345 28.5 18.0 36.0 2.0 39.0 68-69 17.633926967658994 13.0 7.0 29.5 2.0 35.5 70-71 16.503072500683345 14.5 7.5 26.0 2.0 33.5 72-73 18.637234358901964 19.0 7.5 30.0 2.0 35.0 74-75 19.343013657894815 18.5 7.5 31.0 2.0 35.5 76-77 20.25016475380045 23.0 8.5 31.5 2.0 34.5 78-79 23.299666437383046 28.5 12.0 34.0 2.0 36.0 80-81 25.97166955523983 31.5 22.0 35.0 2.0 36.0 82-83 27.002117319123755 33.0 26.0 35.0 2.0 36.0 84-85 27.0028095253829 33.0 26.0 35.0 2.0 36.0 86-87 26.71216429723568 33.0 25.5 35.0 2.0 36.0 88-89 26.27239645441411 33.0 24.5 35.0 2.0 35.0 90-91 24.59708969995885 31.5 11.5 34.5 2.0 35.0 92-93 22.443679731828272 31.0 2.0 34.0 2.0 35.0 94-95 18.043064570874172 23.0 2.0 33.0 2.0 35.0 96-97 16.75146190838067 15.0 2.0 32.5 2.0 34.5 98-99 15.669021179198669 2.0 2.0 32.0 2.0 34.0 100-101 15.05675941139189 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3747814205847071 1101 2 0.42353835815703533 1101 3 0.371918266703247 1101 4 0.22301387641648773 1101 5 0.41926781769218024 1101 6 0.3631873370305456 1101 7 0.33886632616823675 1101 8 0.4078215577544242 1101 9 0.41532164554757856 1101 10-11 0.4863468200770029 1101 12-13 0.5339195804373489 1101 14-15 0.49104312593249233 1101 16-17 0.5750501056090869 1101 18-19 0.5850011037946743 1101 20-21 0.5377003155217821 1101 22-23 0.6255251028201982 1101 24-25 0.5810320677709839 1101 26-27 0.7009232240717935 1101 28-29 0.6030883293889389 1101 30-31 0.7512466514557445 1101 32-33 0.5804687692774593 1101 34-35 0.8630363844998783 1101 36-37 0.6860203595807235 1101 38-39 0.7263838938948979 1101 40-41 0.759975912969928 1101 42-43 0.6816325708566424 1101 44-45 0.7529852675801507 1101 46-47 0.5786951469205377 1101 48-49 0.4502278620192328 1101 50-51 0.37549317032237184 1101 52-53 0.3592992052996635 1101 54-55 0.3793805763506626 1101 56-57 1.1293045121232126 1101 58-59 -8.255936864391945 1101 60-61 -9.662139170139366 1101 62-63 -2.568777325519161 1101 64-65 0.03182859045923081 1101 66-67 -0.9310630057750657 1101 68-69 -3.3777295487305015 1101 70-71 -4.0115175485963235 1101 72-73 -6.781375711398052 1101 74-75 -7.639382279920801 1101 76-77 -8.445390581894847 1101 78-79 -11.547009366277372 1101 80-81 -7.673778820259692 1101 82-83 -3.6958180701359638 1101 84-85 -2.819709929865599 1101 86-87 -2.620368030813008 1101 88-89 -2.669622722850697 1101 90-91 -8.763687803447286 1101 92-93 -14.342146224815048 1101 94-95 -12.498463050815312 1101 96-97 -12.478266043858953 1101 98-99 -11.994300554097421 1101 100-101 -11.87235410889122 1102 1 0.5172105542059562 1102 2 0.5397904888433196 1102 3 0.46540174371968845 1102 4 0.36441581782325017 1102 5 0.46727778440689605 1102 6 0.5041987087776789 1102 7 0.4483574684137679 1102 8 0.5137131093792533 1102 9 0.6870639306736166 1102 10-11 0.7110305530004339 1102 12-13 0.7163000366191099 1102 14-15 0.8361722969633689 1102 16-17 0.7707433263201793 1102 18-19 0.8238131100976958 1102 20-21 0.8935715748962778 1102 22-23 0.8179535176015875 1102 24-25 0.7717754769828602 1102 26-27 0.9800921733046195 1102 28-29 0.9049607125497516 1102 30-31 0.7468119336468106 1102 32-33 0.7499813179751129 1102 34-35 0.8459281454868162 1102 36-37 0.8253546629485129 1102 38-39 0.8034726553428015 1102 40-41 0.8633411168673604 1102 42-43 0.8571317077995317 1102 44-45 0.8709247250896794 1102 46-47 0.8620032659472443 1102 48-49 0.9250500464957057 1102 50-51 0.7509546130998714 1102 52-53 0.7870850766818407 1102 54-55 0.7873699722646066 1102 56-57 1.4763806908783224 1102 58-59 -8.6199925648969 1102 60-61 -9.751530726107188 1102 62-63 -2.624012531420359 1102 64-65 0.23880714776559486 1102 66-67 -1.159928814246772 1102 68-69 -3.5320315207778155 1102 70-71 -4.201981433931779 1102 72-73 -6.7056630041363015 1102 74-75 -7.687180188875539 1102 76-77 -8.576317903290846 1102 78-79 -11.524367782529385 1102 80-81 -7.632348859074046 1102 82-83 -3.3206103179272546 1102 84-85 -2.339750211854497 1102 86-87 -2.0256838665856556 1102 88-89 -2.000566263071132 1102 90-91 -7.849271679629517 1102 92-93 -14.075019266013857 1102 94-95 -12.420705843172726 1102 96-97 -12.268785249912304 1102 98-99 -11.84236124395007 1102 100-101 -11.656577176282756 1103 1 0.3123797241916435 1103 2 0.34130679915489637 1103 3 0.34053076649895075 1103 4 0.2586989126326884 1103 5 0.2935542531628528 1103 6 0.22270815891160822 1103 7 0.23340093746899981 1103 8 0.28644911163770104 1103 9 0.3913997068156121 1103 10-11 0.3400068467400885 1103 12-13 0.4246810713393856 1103 14-15 0.4924786861103172 1103 16-17 0.4860914214655381 1103 18-19 0.5025937235301896 1103 20-21 0.5200827785863495 1103 22-23 0.49849141959109744 1103 24-25 0.469574098975464 1103 26-27 0.47383427161370406 1103 28-29 0.4467495026842343 1103 30-31 0.5550028188194318 1103 32-33 0.560405883505112 1103 34-35 0.45503442225417245 1103 36-37 0.41182045333476935 1103 38-39 0.49413931205636885 1103 40-41 0.49887186958676466 1103 42-43 0.6446898062226225 1103 44-45 0.6106451468932264 1103 46-47 0.476970451238536 1103 48-49 0.4853186988973093 1103 50-51 0.5831354221108711 1103 52-53 0.7204926963718847 1103 54-55 0.6664604439535609 1103 56-57 1.2571539385606627 1103 58-59 -6.506913641976684 1103 60-61 -8.523644093783258 1103 62-63 -2.004671635084435 1103 64-65 0.13362397783283697 1103 66-67 -1.6095079675587485 1103 68-69 -4.408207655448756 1103 70-71 -4.934431112503038 1103 72-73 -7.615060341364014 1103 74-75 -8.26025780884382 1103 76-77 -9.119456809503415 1103 78-79 -12.083735905131732 1103 80-81 -8.233649300109704 1103 82-83 -4.230877647018303 1103 84-85 -3.3159136563165106 1103 86-87 -3.048995169625808 1103 88-89 -3.1435112873216475 1103 90-91 -8.54991677656032 1103 92-93 -14.438649398677438 1103 94-95 -12.477591231584734 1103 96-97 -12.497291123714623 1103 98-99 -12.108907545907822 1103 100-101 -11.879183120865225 1104 1 0.20053272597317928 1104 2 0.20750827471761468 1104 3 0.24113986564224987 1104 4 0.18819515662622877 1104 5 0.08352563888991682 1104 6 0.09161739528611434 1104 7 0.03697159977899389 1104 8 0.10640824215215616 1104 9 0.12556456220593049 1104 10-11 0.21434473473139093 1104 12-13 0.13664294250176567 1104 14-15 0.08075726364413072 1104 16-17 0.306572479565709 1104 18-19 0.2553662644978374 1104 20-21 0.2258340386929163 1104 22-23 0.2807250559331962 1104 24-25 0.3114700517730924 1104 26-27 0.23658204535501426 1104 28-29 0.15909756960959243 1104 30-31 0.26790715012668187 1104 32-33 0.3016877750989124 1104 34-35 0.3024284043930692 1104 36-37 0.2573710175599899 1104 38-39 0.27496671369780046 1104 40-41 0.35613215111700924 1104 42-43 0.28599876962581305 1104 44-45 0.17422392908925133 1104 46-47 0.1802399355271973 1104 48-49 0.1996689034021344 1104 50-51 0.19167038989227336 1104 52-53 0.3663979029567983 1104 54-55 0.2675130966346657 1104 56-57 0.8107664346030248 1104 58-59 -6.596081994004315 1104 60-61 -8.900691523626431 1104 62-63 -2.6930394054492197 1104 64-65 -0.38550132769837475 1104 66-67 -1.925769534585747 1104 68-69 -4.390981270010805 1104 70-71 -4.948315811115435 1104 72-73 -7.804147900442771 1104 74-75 -8.869779058640244 1104 76-77 -9.098523872538482 1104 78-79 -12.21362318269018 1104 80-81 -8.34417008617299 1104 82-83 -4.589626298191078 1104 84-85 -3.701557876742008 1104 86-87 -3.4354366747190035 1104 88-89 -3.61351136519038 1104 90-91 -9.206847035503667 1104 92-93 -14.929141709637427 1104 94-95 -12.432876397641456 1104 96-97 -12.250097421604927 1104 98-99 -11.684949591045507 1104 100-101 -11.284682831839696 1105 1 0.11736378678219594 1105 2 0.20801210196567155 1105 3 0.18463392500373388 1105 4 0.1468472754260759 1105 5 0.10549282169371565 1105 6 0.10303270252215668 1105 7 0.13980487835569733 1105 8 0.15808052282287122 1105 9 0.16392940835140024 1105 10-11 0.16455045728775985 1105 12-13 0.27037797078882164 1105 14-15 0.2542868576547974 1105 16-17 0.25514387003116923 1105 18-19 0.2998988524783144 1105 20-21 0.19655336682163949 1105 22-23 0.3472309153894244 1105 24-25 0.2929586251997449 1105 26-27 0.3680678271048663 1105 28-29 0.19131629491836577 1105 30-31 0.291456020789866 1105 32-33 0.19413523731559934 1105 34-35 0.22915786473460287 1105 36-37 0.1356686132884093 1105 38-39 0.1688712738065803 1105 40-41 0.3095064682538151 1105 42-43 0.2811719853011496 1105 44-45 0.25929771136728874 1105 46-47 0.2863010800417882 1105 48-49 0.3543421703548617 1105 50-51 0.3823240630845035 1105 52-53 0.302208141170496 1105 54-55 0.31511300842762324 1105 56-57 0.8659629794183843 1105 58-59 -6.5683451111160664 1105 60-61 -8.507706684336718 1105 62-63 -2.4278822986602115 1105 64-65 -0.06979532092114837 1105 66-67 -1.543585046666255 1105 68-69 -4.210438892771268 1105 70-71 -4.714349976600403 1105 72-73 -6.045805053041789 1105 74-75 -6.471645812321073 1105 76-77 -8.603954056678148 1105 78-79 -11.93899525024909 1105 80-81 -7.9083812618599225 1105 82-83 -4.019344758000628 1105 84-85 -3.1368351283535993 1105 86-87 -2.842388388982517 1105 88-89 -2.920677326150976 1105 90-91 -8.26405760530561 1105 92-93 -11.426913669903168 1105 94-95 -11.725490391248599 1105 96-97 -12.275616246867333 1105 98-99 -11.797548519538653 1105 100-101 -11.321808446857691 1106 1 0.13120607063549272 1106 2 0.18904512242243499 1106 3 0.1596057042586807 1106 4 0.128576401991495 1106 5 0.15773725334909017 1106 6 0.1294852388931318 1106 7 0.16360611018533433 1106 8 0.22389881349620566 1106 9 0.1450075584689543 1106 10-11 0.22497684602070223 1106 12-13 0.2621148727661975 1106 14-15 0.32912839119596526 1106 16-17 0.26802095061778175 1106 18-19 0.2826537333729888 1106 20-21 0.1732109952302281 1106 22-23 0.11391288019870416 1106 24-25 0.266688330298841 1106 26-27 0.3223201336759445 1106 28-29 0.2806718467238625 1106 30-31 0.35181862290728105 1106 32-33 0.3319384884664487 1106 34-35 0.3396172056835667 1106 36-37 0.3634686019933895 1106 38-39 0.4696570173383279 1106 40-41 0.38737282361266523 1106 42-43 0.24034565196759416 1106 44-45 0.3144993964096514 1106 46-47 0.2442199824615372 1106 48-49 0.24764535729888593 1106 50-51 0.356772523162995 1106 52-53 0.23022927361738965 1106 54-55 0.14165761939145938 1106 56-57 1.190506547945887 1106 58-59 -6.064744066729997 1106 60-61 -8.541287774185253 1106 62-63 -2.1309969091735894 1106 64-65 0.30612731591500975 1106 66-67 -1.1197090777652114 1106 68-69 -3.783983767957924 1106 70-71 -4.536838884956769 1106 72-73 -5.898439020061673 1106 74-75 -6.224027641522872 1106 76-77 -8.355391734717601 1106 78-79 -11.865764727941759 1106 80-81 -7.746457803781595 1106 82-83 -3.5345059708976585 1106 84-85 -2.5672098104290093 1106 86-87 -2.344523769897023 1106 88-89 -2.483580887847676 1106 90-91 -7.904431104093078 1106 92-93 -11.5700809636228 1106 94-95 -11.756755455478947 1106 96-97 -12.269931758494817 1106 98-99 -11.753221948202862 1106 100-101 -11.553667163622258 1107 1 0.04519498195618965 1107 2 0.11446463073057345 1107 3 0.0612248121422887 1107 4 0.09133708807004837 1107 5 0.017956197848775446 1107 6 0.08784108773621568 1107 7 0.1308647087664383 1107 8 0.1014012994093676 1107 9 -0.0016891065571726926 1107 10-11 0.22733038639628944 1107 12-13 0.2026412000094595 1107 14-15 0.19875530334028468 1107 16-17 0.21367177440085783 1107 18-19 0.13515199239677145 1107 20-21 0.1182256093863785 1107 22-23 0.24393396442512483 1107 24-25 0.227986973006999 1107 26-27 0.2928713535311118 1107 28-29 0.3075865020607864 1107 30-31 0.3066245124289537 1107 32-33 0.30649324216852847 1107 34-35 0.26820612771795993 1107 36-37 0.2391932833445196 1107 38-39 0.2607395362630811 1107 40-41 0.20125589529735066 1107 42-43 0.4501252092239483 1107 44-45 0.43941356126001807 1107 46-47 0.40937359013255303 1107 48-49 0.48353587500495365 1107 50-51 0.4142070775232938 1107 52-53 0.4407424263579216 1107 54-55 0.40621820703789524 1107 56-57 1.0823297769210853 1107 58-59 -6.129367681063588 1107 60-61 -8.59019573011177 1107 62-63 -2.3080411751679684 1107 64-65 0.1527599916950173 1107 66-67 -1.253285938517248 1107 68-69 -4.058908520956923 1107 70-71 -4.382249487501941 1107 72-73 -5.755584095275193 1107 74-75 -6.359959842217254 1107 76-77 -8.756056067806528 1107 78-79 -12.232259682458755 1107 80-81 -8.023520190994795 1107 82-83 -3.615558123179511 1107 84-85 -2.6298080332940508 1107 86-87 -2.369364905817047 1107 88-89 -2.4994189586451405 1107 90-91 -8.080641584769914 1107 92-93 -11.669869744705085 1107 94-95 -11.93523284622432 1107 96-97 -12.57060437796094 1107 98-99 -11.833886398472881 1107 100-101 -11.411439618030442 1108 1 -0.07684751770946718 1108 2 -0.014686046652776952 1108 3 -0.017127154102801967 1108 4 -0.02564442440269943 1108 5 -0.17001600559795804 1108 6 -0.01057570714592515 1108 7 -0.09050070875817795 1108 8 -0.052485225977299876 1108 9 -0.06527177431462405 1108 10-11 -0.030340232560163827 1108 12-13 -0.20464336686320195 1108 14-15 -0.03944282842867608 1108 16-17 -0.051593739069495825 1108 18-19 -0.0774101524503763 1108 20-21 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0.6049839602750211 2314 58-59 3.8252657155551653 2314 60-61 3.533236546859971 2314 62-63 0.9648078544752003 2314 64-65 0.7664513631569356 2314 66-67 1.363815331065716 2314 68-69 -0.0724744957108534 2314 70-71 2.72066986057607 2314 72-73 8.231758703865818 2314 74-75 9.533024752069213 2314 76-77 8.570115094003363 2314 78-79 6.113465968799851 2314 80-81 3.091740635331938 2314 82-83 1.5972905777082502 2314 84-85 1.2576074442760223 2314 86-87 1.0638695660667317 2314 88-89 1.3298325239830682 2314 90-91 2.510451609171124 2314 92-93 4.585177061847013 2314 94-95 8.358612147946143 2314 96-97 9.022853389296664 2314 98-99 9.316792657576393 2314 100-101 8.516716991655867 2315 1 -0.6570575968619892 2315 2 -0.4920865712867659 2315 3 -0.4788033282258901 2315 4 -0.3521758864715423 2315 5 -0.657502023599811 2315 6 -0.5958669802733496 2315 7 -0.5696688623820378 2315 8 -0.7142567178355037 2315 9 -0.9651989571171811 2315 10-11 -0.8351959006411889 2315 12-13 -0.7019172370511058 2315 14-15 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3.1339496619695346 2315 82-83 1.3502138744145356 2315 84-85 0.9269873422318433 2315 86-87 0.855807311845961 2315 88-89 0.9313143142530471 2315 90-91 2.057762575850173 2315 92-93 4.245277190787554 2315 94-95 8.15687018628676 2315 96-97 8.967199561118147 2315 98-99 9.11693477116382 2315 100-101 8.272317435973141 2316 1 -0.4742686283084403 2316 2 -0.4277414664712751 2316 3 -0.5427574307304148 2316 4 -0.3118909384243196 2316 5 -0.5520580442854737 2316 6 -0.41891128496577323 2316 7 -0.3691549369650673 2316 8 -0.5749320474023278 2316 9 -0.64710105222386 2316 10-11 -0.5352056937452261 2316 12-13 -0.5126888013097854 2316 14-15 -0.5969156923307111 2316 16-17 -0.7682370391807538 2316 18-19 -0.8989108469873273 2316 20-21 -0.6853006973100406 2316 22-23 -0.7981688395231359 2316 24-25 -2.0398503096901734 2316 26-27 -1.3076544250586366 2316 28-29 -0.9567194910309382 2316 30-31 -0.6444983779523596 2316 32-33 -0.5789980787884019 2316 34-35 -0.6467108231174734 2316 36-37 -0.5517790333283443 2316 38-39 -1.0011879754804553 2316 40-41 -0.8629741288534163 2316 42-43 -0.9638913054435747 2316 44-45 -0.8122659854096241 2316 46-47 -0.7698512734398903 2316 48-49 -0.8814791206921839 2316 50-51 -0.8457313958032344 2316 52-53 -0.7919500721992776 2316 54-55 -0.7613958249973081 2316 56-57 0.46359951199778493 2316 58-59 3.9345188491122194 2316 60-61 3.8537503417426002 2316 62-63 1.2783458849524614 2316 64-65 1.0111686711056755 2316 66-67 6.061519591204782 2316 68-69 8.855679026916786 2316 70-71 5.690662193702973 2316 72-73 9.415872208901181 2316 74-75 10.124535795490953 2316 76-77 8.74834175784802 2316 78-79 6.244191375367905 2316 80-81 3.229311837183964 2316 82-83 1.7714016038877745 2316 84-85 1.482743710336095 2316 86-87 1.3542102578939783 2316 88-89 1.4040798603645719 2316 90-91 2.860542975237177 2316 92-93 4.783912630418925 2316 94-95 8.670184596979706 2316 96-97 9.460565515139326 2316 98-99 9.790222263252538 2316 100-101 8.76308693413551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 32.0 4 76.0 5 196.0 6 444.0 7 957.0 8 2017.0 9 3635.0 10 6189.0 11 9202.0 12 13326.0 13 18363.0 14 22921.0 15 31805.0 16 42347.0 17 54296.0 18 56317.0 19 55152.0 20 57575.0 21 57210.0 22 55302.0 23 53318.0 24 55482.0 25 60189.0 26 79311.0 27 114313.0 28 159414.0 29 208724.0 30 249910.0 31 268415.0 32 275998.0 33 286675.0 34 292112.0 35 286220.0 36 244021.0 37 154223.0 38 51061.0 39 2454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15154483073558 19.45026618437023 13.34025332580246 23.057935659091726 2 15.99114335012304 21.423696554906183 38.78139658951092 23.80376350545986 3 19.644514613938618 26.011735367287436 29.741862771779125 24.601887246994817 4 12.714367672811267 16.46153291621736 37.01698000426528 33.80711940670609 5 12.888883411698899 39.199273564299084 33.72921393874051 14.182629085261514 6 31.620955121485277 36.23174867310864 17.636195974420357 14.511100230985729 7 28.7113459349215 33.51089297460959 20.378648388056025 17.399112702412886 8 27.239377797588975 34.537634499036706 19.840406391193575 18.382581312180744 9 27.749225792305083 15.796720543312077 19.877928998170738 36.57612466621211 10-11 25.239831671777992 27.00687250128409 27.155060810222242 20.598235016715677 12-13 26.12615305132449 24.35758032686433 27.850285803538977 21.665980818272203 14-15 22.753712298795747 25.513651311244367 26.52932953342601 25.203306856533874 16-17 21.831590677668274 29.06826244063907 26.978051850138623 22.122095031554032 18-19 21.69460489952494 27.352083911870253 29.722817425839764 21.230493762765047 20-21 22.772624736799422 26.44116772447517 29.49952090736241 21.286686631362997 22-23 22.22600857260431 26.689109428362883 29.21290636517372 21.871975633859083 24-25 21.919374265967 26.85267676115355 29.606272959651086 21.621676013228363 26-27 21.826129935845035 27.01495207038198 29.56625223833501 21.592665755437974 28-29 21.546864491470924 27.204044526801358 29.68091468640109 21.568176295326634 30-31 21.608434945581333 27.08869231487876 29.5994067733221 21.703465966217802 32-33 21.34580059086258 27.115098655067488 29.43268211091522 22.106418643154715 34-35 21.94393981446438 27.036692607050643 29.343617572177106 21.675750006307876 36-37 22.090487050411912 26.651104216388465 29.340561825586885 21.917846907612738 38-39 21.697262735810014 26.927209843100535 29.571905406942168 21.803622014147287 40-41 21.738114760624676 26.796467951639695 29.70170361933102 21.76371366840461 42-43 21.550388132600855 26.8161829481252 29.37535529246053 22.258073626813417 44-45 21.592969810416538 27.060085466169205 29.18798125422993 22.158963469184332 46-47 21.68419556630287 27.233941289966968 28.72548146196236 22.356381681767807 48-49 21.623638410915397 27.189716185529917 28.68244073082808 22.504204672726605 50-51 21.631864533070537 27.351111965724655 28.586502580866135 22.43052092033867 52-53 21.793854542996417 27.380842578057518 28.42136413823147 22.40393874071459 54-55 21.22963853546765 27.191779978267483 28.71449802667926 22.864083459585604 56-57 21.49909924869956 27.41368934406022 28.399439318602738 22.687772088637477 58-59 19.95853575096152 26.87436819750806 30.145177829185272 23.02191822234515 60-61 21.420744431167744 27.15813151645417 28.64540305830078 22.775720994077307 62-63 21.30818214586087 27.059714045416317 28.599513396611798 23.032590412111016 64-65 20.415968030131467 27.001797643377213 29.14513357198642 23.4371007545049 66-67 20.603945560806267 27.268100495863944 28.805688371570998 23.322265571758795 68-69 21.25309308568079 26.77060257320046 28.753432476628305 23.22287186449045 70-71 20.41917206113009 27.147533586865443 28.959474764961 23.473819587043465 72-73 20.255372972708418 27.09516875997969 28.994001480511695 23.655456786800197 74-75 20.754981410258367 27.185369766583605 28.320960505567772 23.738688317590256 76-77 19.249863112044043 27.554399106667525 29.173199782922882 24.022537998365546 78-79 20.278330349671315 27.579744154563496 28.130511423949496 24.011414071815697 80-81 20.702987544625888 27.83568031471773 27.490477833224773 23.97085430743161 82-83 21.080299789244012 27.705979699961446 27.007344545117444 24.206375965677097 84-85 20.90062220476062 27.37755013615094 27.0761722865443 24.64565537254414 86-87 20.997915970788746 27.37041050989929 27.1305579118616 24.50111560745036 88-89 21.07548669969353 27.323382220821824 27.0475359161891 24.553595163295547 90-91 20.59243824089775 27.6148221243331 27.18563990055086 24.607099734218288 92-93 20.438589589465955 27.41137545581421 27.409058680753063 24.740976273966776 94-95 19.095176776687616 27.484490991496624 28.05735009732962 25.36298213448614 96-97 18.98510574659895 27.309532270476883 28.27206071866662 25.433301264257548 98-99 19.242035828761523 27.379181660022272 27.75764509483727 25.621137416378932 100-101 19.13387523300575 27.475326607609645 27.929600185273777 25.46119797411082 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 841.0 1 616.5 2 425.5 3 518.5 4 586.5 5 714.5 6 1061.0 7 1608.0 8 2117.0 9 2447.0 10 2679.0 11 2898.5 12 3189.5 13 3527.5 14 3883.0 15 4214.0 16 4500.0 17 4840.5 18 5295.0 19 5951.0 20 6718.0 21 7599.5 22 8858.5 23 10433.0 24 12427.0 25 14922.5 26 18366.0 27 22700.0 28 26789.0 29 31473.0 30 37052.5 31 43752.5 32 51504.0 33 59344.0 34 67193.0 35 74969.0 36 82907.5 37 90018.5 38 95730.5 39 100581.5 40 104960.0 41 110507.5 42 116239.5 43 121428.5 44 127010.5 45 131487.5 46 134678.5 47 137833.5 48 140833.5 49 140046.5 50 134723.5 51 127775.5 52 120503.0 53 113548.0 54 105127.5 55 94705.5 56 84222.5 57 73692.5 58 63200.0 59 53294.5 60 44095.5 61 35011.0 62 26873.0 63 20143.5 64 14564.0 65 10454.0 66 7412.0 67 5292.0 68 3740.0 69 2657.5 70 2031.0 71 1659.0 72 1368.0 73 1107.0 74 812.5 75 538.5 76 333.0 77 192.5 78 111.5 79 64.0 80 42.5 81 24.0 82 13.5 83 8.0 84 5.0 85 5.0 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02072563761372818 2 0.005586911008918031 3 0.0038447559631263877 4 0.0 5 0.0685147527491507 6 0.0 7 0.0 8 1.2014862384769962E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.802229357715494E-4 16-17 0.0 18-19 0.0015168763760772075 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.6520435779058697E-4 28-29 0.0020725637613728184 30-31 0.008921035320691696 32-33 2.8535298163828655E-4 34-35 0.0011414119265531462 36-37 0.02701842178775145 38-39 0.026087269952931775 40-41 0.02832503807209518 42-43 0.029015892659219458 44-45 0.02981187729221046 46-47 0.031223623622420933 48-49 0.032064663989354834 50-51 0.026132325686874663 52-53 0.0220773096320148 54-55 0.01673069587079217 56-57 0.15140228462608246 58-59 0.7412118790944399 60-61 0.3037357210869846 62-63 3.00371559619249E-4 64-65 0.13755515572763508 66-67 1.3802223350284302 68-69 2.6465738118052027 70-71 2.13984699072753 72-73 1.8043319586328286 74-75 1.517477119196446 76-77 1.0924813994911706 78-79 0.4101123089261417 80-81 1.3516720182866205E-4 82-83 1.3516720182866205E-4 84-85 1.5018577980962452E-5 86-87 0.002463046788877842 88-89 3.0037155961924904E-5 90-91 0.21960164723763292 92-93 0.1689439837078466 94-95 1.2593678380156252 96-97 1.2313882272370922 98-99 0.9670161990382102 100-101 0.39157938369763395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3329210.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.174540136001156 #Duplication Level Percentage of deduplicated Percentage of total 1 69.6915799081795 30.785934937952074 2 14.581206238849193 12.88236160458708 3 5.985447247625899 7.932131390165038 4 3.073448847714646 5.430727579172685 5 1.7740405759618338 3.918371331286031 6 1.1193583775084983 2.9668284944290972 7 0.7495234710508282 2.3176898258366796 8 0.5347973977638415 1.889954328971823 9 0.3891928379767686 1.5473173177664064 >10 1.8609457930077191 14.542064664603597 >50 0.14327985161349652 4.403212152268566 >100 0.0895434731995443 7.440695675208387 >500 0.005590627884516783 1.6465231638791156 >1k 0.0018408164974277584 1.3074002288197297 >5k 6.817838879362068E-5 0.227537634847571 >10k+ 1.3635677758724136E-4 0.7612496702061158 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14165 0.42547631420066623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11111 0.33374283989294756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7555 0.2269307132923426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.00371559619249E-5 0.0 0.0 0.0 0.0 8 6.00743119238498E-5 0.0 0.0 0.0 0.0 9 6.00743119238498E-5 0.0 0.0 0.0 0.0 10-11 9.01114678857747E-5 0.0 0.0 0.0 0.0 12-13 1.201486238476996E-4 0.0 0.0 1.501857798096245E-4 0.0 14-15 1.802229357715494E-4 0.0 0.0 1.6520435779058694E-4 0.0 16-17 1.802229357715494E-4 0.0 0.0 3.4542729356213634E-4 0.0 18-19 2.553158256763617E-4 0.0 0.0 4.956130733717608E-4 0.0 20-21 3.00371559619249E-4 0.0 0.0 7.959846329910099E-4 0.0 22-23 3.00371559619249E-4 0.0 0.0 0.0015619321100200948 0.0 24-25 3.604458715430988E-4 0.0 0.0 0.0028835669723447904 0.0 26-27 3.904830275050237E-4 0.0 0.0 0.005496799541032257 0.0 28-29 5.857245412575356E-4 0.0 0.0 0.013757017430561605 0.0 30-31 6.908545871242727E-4 0.0 0.0 0.045281012612601784 0.0 32-33 6.908545871242727E-4 0.0 0.0 0.11666431375611631 0.0 34-35 7.959846329910099E-4 0.0 0.0 0.23364401764983284 0.0 36-37 9.611889907815968E-4 0.0 0.0 0.4312134109893939 0.0 38-39 0.0011564305045341087 0.0 0.0 0.7281907719849454 0.0 40-41 0.0011714490825150711 0.0 0.0 1.0601764382541203 0.0 42-43 0.001231523394438921 0.0 0.0 1.375866947413951 0.0 44-45 0.0012915977063627708 0.0 0.0 1.7450235941860082 0.0 46-47 0.0014417834861723952 0.0 0.0 2.15950630930461 0.0 48-49 0.00153189495405817 0.0 0.0 2.573853857221383 0.0 50-51 0.00153189495405817 0.0 0.0 2.999750691605516 0.0 52-53 0.00153189495405817 1.501857798096245E-5 0.0 3.3883263597069577 0.0 54-55 0.00153189495405817 3.00371559619249E-5 0.0 3.7930920548718765 0.0 56-57 0.0015619321100200948 3.00371559619249E-5 0.0 4.1476206066904755 0.0 58-59 0.0015619321100200948 4.505573394288735E-5 0.0 4.396117997963481 0.0 60-61 0.0015619321100200948 9.01114678857747E-5 0.0 4.588145536028067 0.0 62-63 0.0015619321100200948 1.0513004586673716E-4 0.0 4.7491146548280225 0.0 64-65 0.0015619321100200948 1.201486238476996E-4 0.0 4.907725856884967 0.0 66-67 0.0015919692659820198 1.201486238476996E-4 0.0 5.03604458715431 0.0 68-69 0.0015919692659820198 1.3516720182866205E-4 0.0 5.186440627055667 0.0 70-71 0.0016220064219439447 1.501857798096245E-4 0.0 5.4642242453915495 0.0 72-73 0.001667062155886832 1.501857798096245E-4 0.0 5.799033404321145 0.0 74-75 0.001697099311848757 1.501857798096245E-4 0.0 6.1695417231114895 0.0 76-77 0.0017121178898297194 1.501857798096245E-4 0.0 6.694576190747956 0.0 78-79 0.001727136467810682 1.501857798096245E-4 0.0 7.449830440254595 0.0 80-81 0.0018623036696393438 1.501857798096245E-4 0.0 8.311551389068278 0.0 82-83 0.0018923408256012687 1.501857798096245E-4 0.0 9.107521003481306 0.0 84-85 0.0019524151375251183 1.501857798096245E-4 0.0 9.73082202684721 0.0 86-87 0.002012489449448968 1.501857798096245E-4 0.0 10.283625845170477 0.0 88-89 0.002012489449448968 1.501857798096245E-4 0.0 10.840860143998125 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13265 0.0 32.571888 1 GTATCAA 19990 0.0 30.86382 1 GTACATG 22795 0.0 29.17657 1 TACATGG 23095 0.0 28.506067 2 ACATGGG 23240 0.0 27.129736 3 ATCAACG 24060 0.0 25.323116 3 TCAACGC 24150 0.0 25.22685 4 TATCAAC 24725 0.0 24.73851 2 CAACGCA 24680 0.0 24.666 5 AACGCAG 25095 0.0 24.29803 6 CATGGGA 15910 0.0 24.184526 4 CATGGGG 7045 0.0 22.22157 4 ATGGGAG 5695 0.0 21.941675 5 GAGTACT 13070 0.0 21.109692 12-13 ATGGGAT 6235 0.0 20.948887 5 ACGCAGA 29025 0.0 20.89442 7 CGCAGAG 28945 0.0 20.88705 8 AGTACAT 16520 0.0 20.525263 2 GTCGGGA 975 0.0 20.314892 2 TACCTGG 2565 0.0 20.224379 2 >>END_MODULE