FastQCFastQC Report
Fri 27 May 2016
SRR939836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465405
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128900.371962295893265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90050.2598541873172111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62660.18081580652189283No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35880.10353768174282658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201450.031.6414361
GTACATG224450.031.3123151
TACATGG226450.030.7241572
ACATGGG236600.028.731773
GGTATCA136100.027.9278451
ATCAACG244150.026.0512923
GTCGGGA9600.025.9710082
CATGGGA162800.025.8922124
TATCAAC245650.025.833272
TCAACGC247000.025.808214
CAACGCA249800.025.3490625
AACGCAG255950.024.758716
TACCTGG26500.024.1422212
CATGGGG72950.024.0546534
ATGGGAG60050.022.954295
GAGTACT122450.021.99494412-13
ACGCAGA290700.021.6696117
CGCAGAG291800.021.4911888
AGTACAT159600.021.487152
ATGGGAT65250.021.0528855
Produced by FastQC (version 0.11.5)