FastQCFastQC Report
Fri 27 May 2016
SRR939836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465405
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146240.42199973740443036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117160.3380845817444137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.22487992024020279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG231250.029.9201241
GTATCAA198750.029.6075291
TACATGG234800.029.2391012
GGTATCA137900.029.1134511
ACATGGG239700.027.8044383
GTCGGGA9800.025.6592162
CATGGGA168350.025.0492364
ATCAACG242800.024.0500073
TCAACGC243050.024.025274
TATCAAC246150.023.8045882
CAACGCA246850.023.6554245
AACGCAG251850.023.2422986
ATGGGAG59150.022.9359725
CATGGGG73150.022.1777574
AGTACAT171650.022.0020352
ATGGGAT66400.021.146065
GAGTACA204400.020.4542061
GAGTACT128500.020.43274112-13
ACGCAGA289750.020.1530347
CGCAGAG291750.019.9335868
Produced by FastQC (version 0.11.5)