##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939836_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3465405 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40021440495411 31.0 31.0 34.0 30.0 34.0 2 31.618461622811765 31.0 31.0 34.0 30.0 34.0 3 31.663243690131456 31.0 31.0 34.0 30.0 34.0 4 35.47332793713866 37.0 35.0 37.0 33.0 37.0 5 35.220414352723566 37.0 35.0 37.0 32.0 37.0 6 35.253157134591774 37.0 35.0 37.0 32.0 37.0 7 35.153510484344544 37.0 35.0 37.0 32.0 37.0 8 35.12152172689772 37.0 35.0 37.0 32.0 37.0 9 36.68956153754034 39.0 35.0 39.0 32.0 39.0 10-11 36.563232291752335 38.5 35.0 39.0 32.0 39.0 12-13 36.48048352212801 38.0 35.0 39.0 32.0 39.0 14-15 37.493704920492696 39.0 36.0 41.0 32.0 41.0 16-17 37.428546014102245 39.0 36.0 41.0 32.0 41.0 18-19 37.43891146922221 39.0 36.0 41.0 32.0 41.0 20-21 37.365309538134795 39.0 36.0 41.0 31.5 41.0 22-23 37.23398361807639 39.0 36.0 40.0 31.0 41.0 24-25 37.08139005397638 39.0 36.0 40.0 31.0 41.0 26-27 36.78742369218028 39.0 35.5 40.0 30.5 41.0 28-29 36.563095511202874 38.0 35.0 40.0 30.0 41.0 30-31 36.29521801925027 38.0 35.0 40.0 30.0 41.0 32-33 36.258683184216565 38.0 35.0 40.0 30.0 41.0 34-35 36.09612339683241 38.0 35.0 40.0 30.0 41.0 36-37 36.102141308158785 38.0 35.0 40.0 30.0 41.0 38-39 35.99738342271682 38.0 35.0 40.0 30.0 41.0 40-41 35.90404757885442 38.0 35.0 40.0 29.0 41.0 42-43 35.6628476325278 38.0 34.5 40.0 28.0 41.0 44-45 35.38398830728299 38.0 34.0 40.0 27.0 41.0 46-47 35.05462132131742 38.0 34.0 40.0 26.0 41.0 48-49 34.73219133117197 38.0 33.5 40.0 25.0 41.0 50-51 34.512753343404306 38.0 33.0 40.0 24.0 41.0 52-53 34.264580763287405 38.0 33.0 40.0 24.0 41.0 54-55 34.011000157268775 37.5 33.0 40.0 23.5 41.0 56-57 33.66331756317083 37.0 32.5 40.0 23.0 41.0 58-59 33.311846955839215 37.0 32.0 40.0 22.0 41.0 60-61 33.0150729856972 36.0 31.5 39.0 21.5 41.0 62-63 32.7338318320658 36.0 31.0 39.0 20.5 40.5 64-65 32.304708107710354 35.5 31.0 39.0 19.5 40.0 66-67 31.41495626052366 35.0 30.0 38.5 12.5 40.0 68-69 29.550491645276672 34.0 26.5 37.0 7.5 40.0 70-71 28.906132327967438 34.0 25.5 37.0 7.5 39.0 72-73 29.45440648351347 34.0 27.0 36.0 7.5 39.0 74-75 29.546703199193168 34.0 28.0 36.0 7.5 39.0 76-77 29.028818853784767 32.5 27.5 35.0 7.0 37.5 78-79 29.53437650144788 34.0 29.0 35.0 7.0 37.5 80-81 29.336256656869832 34.0 29.0 35.0 3.5 37.0 82-83 28.894594859763867 34.0 28.5 35.0 2.0 36.5 84-85 28.54405603385463 33.0 28.0 35.0 2.0 36.0 86-87 28.156253598064296 33.0 27.0 35.0 2.0 36.0 88-89 27.703182889157254 33.0 26.5 35.0 2.0 35.0 90-91 27.213189223193247 33.0 26.0 35.0 2.0 35.0 92-93 26.735349403605063 32.0 25.0 34.5 2.0 35.0 94-95 25.94581571273776 32.0 24.0 34.0 2.0 35.0 96-97 25.43283368033462 32.0 22.0 34.0 2.0 35.0 98-99 24.76711481053441 32.0 19.0 34.0 2.0 35.0 100-101 23.316832664580332 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2669644718810318 1101 2 0.32892992616211814 1101 3 0.28262324883555934 1101 4 0.2258243009446943 1101 5 0.26613054279057025 1101 6 0.23959353306936748 1101 7 0.1723278152479537 1101 8 0.23296591835526215 1101 9 0.3757008984835082 1101 10-11 0.347651945984488 1101 12-13 0.38780339996720414 1101 14-15 0.41764660912323137 1101 16-17 0.38367462876109926 1101 18-19 0.37941381800479235 1101 20-21 0.43607997285066347 1101 22-23 0.4239888444267237 1101 24-25 0.2988113502182088 1101 26-27 0.35241445629133494 1101 28-29 0.363508417263084 1101 30-31 0.13344966571296624 1101 32-33 0.13804992339060362 1101 34-35 0.13700904734609054 1101 36-37 0.37935819213809907 1101 38-39 0.32011321623416933 1101 40-41 0.38762681058890536 1101 42-43 0.26394367249013584 1101 44-45 0.10211002717370832 1101 46-47 0.06544422581742282 1101 48-49 0.18025136526637198 1101 50-51 0.2932521386317646 1101 52-53 0.0527262455922326 1101 54-55 -0.05538943211127645 1101 56-57 -0.10186144998642277 1101 58-59 -0.02574253885943989 1101 60-61 -0.17461506681254946 1101 62-63 -0.16028593079997933 1101 64-65 -0.26235504259063447 1101 66-67 -0.3544153444555498 1101 68-69 -8.775010269421134 1101 70-71 -6.262161363456581 1101 72-73 -1.5513201810445878 1101 74-75 -0.47098600697291815 1101 76-77 -0.31102318994845035 1101 78-79 -0.45756545634856494 1101 80-81 -0.5008527932984315 1101 82-83 -0.40805812133097064 1101 84-85 -0.3385258615533715 1101 86-87 -0.44920456076633286 1101 88-89 -0.25606319457457616 1101 90-91 -0.4974748310054373 1101 92-93 -0.7659643195789236 1101 94-95 -0.5427736390638707 1101 96-97 -1.1226238072328876 1101 98-99 -0.9128741264228069 1101 100-101 -0.9065298716975647 1102 1 0.24236719674074791 1102 2 0.12580138669037666 1102 3 0.2324221908189621 1102 4 0.18273049150133147 1102 5 0.31946143910882085 1102 6 0.23590613784627834 1102 7 0.26203269972857157 1102 8 0.2454874077953022 1102 9 0.32688497421349183 1102 10-11 0.39319076002119147 1102 12-13 0.34343319105810366 1102 14-15 0.4771451103845905 1102 16-17 0.4210708046127607 1102 18-19 0.32688571820406764 1102 20-21 0.3675766495977868 1102 22-23 0.3705541698891395 1102 24-25 0.1774235179815733 1102 26-27 0.3785940822471119 1102 28-29 0.27461641232171985 1102 30-31 0.13590253164526445 1102 32-33 0.09822015043205568 1102 34-35 -0.04357723080637044 1102 36-37 -0.014663780817016914 1102 38-39 0.26091126689554045 1102 40-41 0.19048262995789145 1102 42-43 0.15320269942214537 1102 44-45 0.23661433258125442 1102 46-47 0.1313198110706182 1102 48-49 0.09096616520255907 1102 50-51 0.07096145724935354 1102 52-53 0.10560357683171873 1102 54-55 -0.08948363197265508 1102 56-57 -0.20924447774865484 1102 58-59 -0.14417664387718077 1102 60-61 -0.043015654328009134 1102 62-63 -0.13504500853161971 1102 64-65 -0.12595470185286217 1102 66-67 0.20021502264932423 1102 68-69 -4.589136685391409 1102 70-71 0.0040509605423544315 1102 72-73 0.2915380616675911 1102 74-75 -0.01748215265241626 1102 76-77 -0.19227275148654144 1102 78-79 -0.20978156353142907 1102 80-81 -0.16724482579720856 1102 82-83 -0.2225717509701326 1102 84-85 -0.30245496218799417 1102 86-87 -0.3968764124132953 1102 88-89 -0.47961506544080734 1102 90-91 -0.5563472449773919 1102 92-93 -0.6667046611763183 1102 94-95 -0.42733901938476393 1102 96-97 -0.957443360878024 1102 98-99 -0.66714234214923 1102 100-101 -0.7326904595411499 1103 1 0.2637142926846181 1103 2 0.27150238723008613 1103 3 0.2624643297320688 1103 4 0.23526920533036844 1103 5 0.257607534512978 1103 6 0.22031162628402257 1103 7 0.1637872768863744 1103 8 0.2270049276192765 1103 9 0.3413455753676402 1103 10-11 0.42129201796115723 1103 12-13 0.4370716163497548 1103 14-15 0.37192107874926705 1103 16-17 0.4653983983355161 1103 18-19 0.3590711294217357 1103 20-21 0.35898020753359816 1103 22-23 0.2359691240137778 1103 24-25 0.27376179173920434 1103 26-27 0.2217978851283462 1103 28-29 0.12576148020065858 1103 30-31 0.3153963976310692 1103 32-33 0.3259506170738078 1103 34-35 0.056135529188644284 1103 36-37 0.2080419318080473 1103 38-39 0.30410884235118374 1103 40-41 0.2811500542946703 1103 42-43 0.3589088375879541 1103 44-45 0.3113797662176552 1103 46-47 0.4032727836284238 1103 48-49 0.28301737539892713 1103 50-51 0.3639566413735693 1103 52-53 0.44035235210468926 1103 54-55 0.43034942112652885 1103 56-57 0.38191335635272594 1103 58-59 0.38890136500727834 1103 60-61 0.21920826313784403 1103 62-63 0.26843767922525785 1103 64-65 0.17649579011375494 1103 66-67 0.424395856709193 1103 68-69 -3.8462087055682694 1103 70-71 0.655055651869624 1103 72-73 0.9112629132472811 1103 74-75 0.7758996508100324 1103 76-77 0.5500765967095802 1103 78-79 0.2480496836143331 1103 80-81 0.3045410441741474 1103 82-83 0.06424790278500225 1103 84-85 0.17645482317340466 1103 86-87 0.01923108333129875 1103 88-89 0.04920623858211215 1103 90-91 -0.018454657689641607 1103 92-93 0.064034430449869 1103 94-95 -0.18607038900323047 1103 96-97 -0.40963489573085 1103 98-99 0.08320146363812952 1103 100-101 -0.16930434877600575 1104 1 0.13313277810727797 1104 2 0.18492986181688664 1104 3 0.1989193085359915 1104 4 0.12584234414322992 1104 5 0.20249675869236228 1104 6 0.16080893486304149 1104 7 0.15362930697934019 1104 8 0.041641429837646626 1104 9 0.26323994716581467 1104 10-11 0.18284556957119946 1104 12-13 0.23241145210939607 1104 14-15 0.3353636121678534 1104 16-17 0.28949199311745843 1104 18-19 0.21550636767292985 1104 20-21 0.31261768703279813 1104 22-23 0.30185500568482126 1104 24-25 0.31852827100707515 1104 26-27 0.24583093593394523 1104 28-29 0.15716872884325284 1104 30-31 0.1014865043522093 1104 32-33 0.27358738765453694 1104 34-35 0.24875382630280995 1104 36-37 0.2459487639366742 1104 38-39 0.45770827677590376 1104 40-41 0.4277487564085618 1104 42-43 0.39526308304139235 1104 44-45 0.4383180831633382 1104 46-47 0.3616991178376665 1104 48-49 0.49775063101763806 1104 50-51 0.47262097660168934 1104 52-53 0.38921722158831784 1104 54-55 0.41784709813521914 1104 56-57 0.464299542774242 1104 58-59 0.4362949362669042 1104 60-61 0.4187709234729766 1104 62-63 0.524650137736316 1104 64-65 0.5603238068834813 1104 66-67 0.6614831927352434 1104 68-69 -3.532090815221256 1104 70-71 0.8796379033063033 1104 72-73 1.1615951172494512 1104 74-75 0.8326376576769903 1104 76-77 0.7331987327699423 1104 78-79 0.44868831128853515 1104 80-81 0.37511327653306026 1104 82-83 0.4123733300204684 1104 84-85 0.3458889359921571 1104 86-87 0.4270564938022403 1104 88-89 0.27918717055972664 1104 90-91 0.2259346685064223 1104 92-93 0.08911965011712653 1104 94-95 0.4778871499645412 1104 96-97 0.17943102783851472 1104 98-99 0.34027480973329105 1104 100-101 0.4231465630062061 1105 1 0.19259226954646635 1105 2 0.1772798355881804 1105 3 0.14690940079004378 1105 4 0.1432947281945971 1105 5 0.2449348845822641 1105 6 0.18654619816376083 1105 7 0.17569399919631934 1105 8 0.2761759984838932 1105 9 0.26858374042839017 1105 10-11 0.2091668850295676 1105 12-13 0.13716736737291768 1105 14-15 0.14256112418770073 1105 16-17 0.2417820550014298 1105 18-19 0.12012459208810355 1105 20-21 0.23799417743466478 1105 22-23 0.16463110719188734 1105 24-25 0.19067133381003742 1105 26-27 0.1193419539627314 1105 28-29 0.2807345313430645 1105 30-31 0.2956287862102229 1105 32-33 0.22289999833771645 1105 34-35 0.2505185252248694 1105 36-37 0.2204536330100737 1105 38-39 0.40883895095225853 1105 40-41 0.3035783920859103 1105 42-43 0.36584307270768335 1105 44-45 0.3496435991589095 1105 46-47 0.30629004862149856 1105 48-49 0.24353049131526916 1105 50-51 0.33564382515753266 1105 52-53 0.2795056584757347 1105 54-55 0.4761675143236559 1105 56-57 0.3344821182360036 1105 58-59 0.30465238817765083 1105 60-61 0.15779781874352494 1105 62-63 0.28688608565754237 1105 64-65 0.3034248288169934 1105 66-67 0.7814032444827141 1105 68-69 1.9454291324002178 1105 70-71 2.2185615008593444 1105 72-73 1.3284272811374542 1105 74-75 0.888029214794436 1105 76-77 0.6276825794365308 1105 78-79 0.5842502169519648 1105 80-81 0.6145907928126064 1105 82-83 0.4385991966524294 1105 84-85 0.12307465495452519 1105 86-87 0.1661705551498045 1105 88-89 0.4092507505217853 1105 90-91 0.34779848432962623 1105 92-93 0.35284779450516623 1105 94-95 0.6033286796700317 1105 96-97 0.11256604848622587 1105 98-99 0.44242639193563704 1105 100-101 0.6563622024147371 1106 1 0.3722683632540047 1106 2 0.368524698704892 1106 3 0.41561238573153503 1106 4 0.24754318220276872 1106 5 0.39743172621586353 1106 6 0.38072858059888404 1106 7 0.3669270793762962 1106 8 0.3511608652931457 1106 9 0.512524302980637 1106 10-11 0.4173940716667275 1106 12-13 0.5386142622164343 1106 14-15 0.5883894874589615 1106 16-17 0.5998166694525366 1106 18-19 0.6087409890403066 1106 20-21 0.5506349866402971 1106 22-23 0.5477730438669042 1106 24-25 0.6368109491635394 1106 26-27 0.6177472745395178 1106 28-29 0.6458157568514622 1106 30-31 0.7378088369515368 1106 32-33 0.7738154785857319 1106 34-35 0.7675854452523367 1106 36-37 0.7718225073869647 1106 38-39 0.7541195736223827 1106 40-41 0.7419815552235747 1106 42-43 0.6274247660069605 1106 44-45 0.7935229651207436 1106 46-47 0.8662797592964679 1106 48-49 0.9042346877418055 1106 50-51 0.8516329383878158 1106 52-53 0.7106280039439739 1106 54-55 0.8088166213720456 1106 56-57 0.8357694455630877 1106 58-59 0.9036623310677498 1106 60-61 1.0083053770410544 1106 62-63 1.0136192871934782 1106 64-65 0.9129964319394688 1106 66-67 1.4009651598737598 1106 68-69 2.7926422811618714 1106 70-71 3.236755143467107 1106 72-73 2.094569344354568 1106 74-75 1.628612013781268 1106 76-77 1.0507863045455963 1106 78-79 1.0202324429727554 1106 80-81 1.079476853059095 1106 82-83 1.014199680416663 1106 84-85 1.1779114052703612 1106 86-87 1.183680082110527 1106 88-89 1.2081704128825628 1106 90-91 1.0540113696347682 1106 92-93 1.1734707550509995 1106 94-95 1.5270400972942362 1106 96-97 1.535218246481051 1106 98-99 1.7236811087406316 1106 100-101 1.7483541203158808 1107 1 0.133368020874979 1107 2 0.1402663298309541 1107 3 0.1696188298311334 1107 4 0.07674746194884108 1107 5 0.08573273553086835 1107 6 0.13152829590967485 1107 7 0.09630943066602526 1107 8 0.12214547289558197 1107 9 0.17233278870005364 1107 10-11 0.15320072946541785 1107 12-13 0.17209152194531185 1107 14-15 0.15679663741818928 1107 16-17 0.15042242077747403 1107 18-19 0.14834455749399922 1107 20-21 0.12067092558880432 1107 22-23 0.08763528893978645 1107 24-25 0.22280359574128994 1107 26-27 0.15552180426318074 1107 28-29 0.23610188872046933 1107 30-31 0.28010095279294234 1107 32-33 0.2801819793382876 1107 34-35 0.24455771886205468 1107 36-37 0.17794376431930203 1107 38-39 0.2884185115368538 1107 40-41 0.13889331167218444 1107 42-43 0.337451464316068 1107 44-45 0.34219661286770275 1107 46-47 0.2584034516896736 1107 48-49 0.2737173229091354 1107 50-51 0.19327444133085692 1107 52-53 0.31234456161615043 1107 54-55 0.180403535142581 1107 56-57 0.18077461412649143 1107 58-59 0.1412190208749422 1107 60-61 0.19386537486133193 1107 62-63 0.20752588137271744 1107 64-65 0.17519029952718057 1107 66-67 0.6352862180139276 1107 68-69 1.9992567007001476 1107 70-71 2.3061812234036907 1107 72-73 1.22864780879517 1107 74-75 0.6593756907640262 1107 76-77 0.4556767460739124 1107 78-79 0.3930732364785001 1107 80-81 0.4305894730826445 1107 82-83 0.46657411397407955 1107 84-85 0.452468789574791 1107 86-87 0.3520452276576407 1107 88-89 0.44982178752498214 1107 90-91 0.381206940753156 1107 92-93 0.4463542154990279 1107 94-95 0.8991729359162939 1107 96-97 0.36779600557548164 1107 98-99 0.7003790534561354 1107 100-101 0.8131507293540174 1108 1 -0.18714701501810893 1108 2 -0.23307012504464097 1108 3 -0.21139420353685523 1108 4 -0.1499285321834023 1108 5 -0.07021942311779839 1108 6 -0.037972378101905235 1108 7 -0.11532974134489393 1108 8 -0.09435159926500347 1108 9 -0.3004638065892067 1108 10-11 -0.20964279115116113 1108 12-13 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-0.6845431286505885 2316 64-65 -0.6051774579732729 2316 66-67 -0.04773598208242902 2316 68-69 1.2166911303514603 2316 70-71 1.6872190922095704 2316 72-73 0.6451734703137895 2316 74-75 0.060448937324235175 2316 76-77 -0.17004748615128307 2316 78-79 -0.3897439683716044 2316 80-81 -0.5979019585793814 2316 82-83 -0.3569169891093118 2316 84-85 -0.504761855134312 2316 86-87 -0.5251805380133163 2316 88-89 -0.5441549405977106 2316 90-91 -0.466056235604686 2316 92-93 -0.5792549432876655 2316 94-95 -0.11744274196506765 2316 96-97 -0.12911689177419206 2316 98-99 -0.41816656295029375 2316 100-101 -0.437211770888279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 26.0 4 70.0 5 197.0 6 424.0 7 1042.0 8 2124.0 9 3738.0 10 6145.0 11 9630.0 12 13591.0 13 17410.0 14 21096.0 15 26143.0 16 31915.0 17 38204.0 18 33606.0 19 26589.0 20 26217.0 21 27454.0 22 29749.0 23 33949.0 24 40565.0 25 49199.0 26 61270.0 27 75115.0 28 92355.0 29 112604.0 30 134428.0 31 158854.0 32 189275.0 33 230104.0 34 284781.0 35 360854.0 36 457149.0 37 518965.0 38 321915.0 39 28646.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1159348006732 19.462183195526688 13.378140336202609 23.0437416675975 2 16.036445430423395 21.447322429260485 38.6427549452646 23.873477195051517 3 19.71364930061485 25.961699670543176 29.776813707850835 24.547837320991135 4 12.711183829884243 16.483210476120398 36.97028197281414 33.83532372118122 5 12.887296001477461 39.13277091710781 33.77873581875711 14.201197262657612 6 31.634031703085704 36.246509786449145 17.609692838122193 14.509765672342963 7 28.668943456825392 33.542948082547355 20.393951067768416 17.394157392858844 8 27.260219224015664 34.54897768081941 19.8317079821839 18.359095112981024 9 27.682218961420098 15.77806923000342 19.87995631102281 36.659755497553675 10-11 25.23135102534913 27.00160587290663 27.16321757485777 20.603825526886467 12-13 26.13319366711827 24.353040986551356 27.872586321079357 21.641179025251017 14-15 22.768204582148407 25.484409470177365 26.504564401563453 25.242821546110772 16-17 21.806807016227587 29.084678658565615 26.972884764940215 22.13562956026658 18-19 21.71355151850938 27.339950164555084 29.760792750053746 21.185705566881794 20-21 22.74745664648144 26.412251959006234 29.537168094349724 21.303123300162607 22-23 22.222958066950326 26.67960887688452 29.21307899076731 21.88435406539784 24-25 21.953489687420237 26.790502173844978 29.63128247547508 21.624725663259703 26-27 21.84594985135183 26.98974648651417 29.58099323382094 21.58331042831306 28-29 21.531617620642226 27.199691002615427 29.71118930952157 21.55750206722078 30-31 21.650234663206934 27.059233125718535 29.624282043705303 21.666250167369235 32-33 21.35220903479229 27.103280287025477 29.464912070600924 22.07959860758131 34-35 21.960620447061135 27.02610513940378 29.342558766251052 21.670715647284034 36-37 22.10619041098977 26.647997393292233 29.35690520469184 21.888906991026154 38-39 21.67992929167473 26.92012963192966 29.55802964088374 21.84191143551187 40-41 21.737516548457553 26.811736543067298 29.669828121357572 21.78091878711758 42-43 21.57989478499212 26.846711442431808 29.376574430092596 22.196819342483472 44-45 21.57248446809368 27.07771968492656 29.217618266275736 22.132177580704028 46-47 21.69938093287107 27.176667146608445 28.779191303397194 22.344760617123292 48-49 21.66154035450839 27.14475436593406 28.716109367359454 22.4775959121981 50-51 21.64754966629191 27.325547894313857 28.612174370149894 22.41472806924434 52-53 21.763733391858292 27.34575833619701 28.462593599025098 22.427914672919595 54-55 21.22021173016531 27.212413910990634 28.711688008694658 22.855686350149394 56-57 21.45618715725831 27.488700664862044 28.340702647973842 22.7144095299058 58-59 21.41276613429466 26.965225590220648 28.515616319276592 23.1063919562081 60-61 21.345270411058053 27.207279070076172 28.636894389646578 22.810556129219194 62-63 21.384470321390516 27.046452252417335 28.517263494917156 23.051813931274992 64-65 20.99315555228496 27.05541571344367 28.513837875977483 23.437590858293888 66-67 20.887889112051642 27.33198453052949 28.404899792795334 23.375226564623535 68-69 20.902787235337243 27.150233694282093 28.52187279960824 23.42510627077242 70-71 20.798683346365255 27.307424775789656 28.312818802815126 23.581073075029963 72-73 21.013010737316552 27.17980910955422 28.055165722804848 23.75201443032438 74-75 21.09560606710077 27.426666395332006 27.525284998815795 23.952442538751427 76-77 20.996333934265408 27.429419827525614 27.683609622091133 23.890636616117845 78-79 21.131556513904755 27.465414874331138 27.448705968859677 23.95432264290443 80-81 20.9619079155728 27.844281353772814 27.26318606837255 23.930624662281833 82-83 21.072611024949907 27.708764650350243 27.023433201934495 24.195191122765358 84-85 20.922081186267977 27.399405954136753 27.058996839893013 24.61951601970226 86-87 21.013034536999722 27.369973859937918 27.120621012962392 24.49637059009996 88-89 21.095507328304762 27.30984224327415 27.026440533976032 24.568209894445054 90-91 20.907202096162614 27.473783538890395 27.077549936803468 24.541464428143524 92-93 20.98601639248465 27.335957920040283 27.040847024398268 24.637178663076796 94-95 20.582730882464 27.068349673761787 27.280595472272534 25.068323971501684 96-97 20.8694495196911 26.91168599413271 27.127340484007522 25.09152400216867 98-99 20.76028738066393 27.058995004679282 26.874753318608814 25.30596429604797 100-101 20.607898437325264 27.09280739613613 27.137034720253155 25.162259446285447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1421.0 1 987.5 2 705.5 3 946.0 4 874.0 5 864.0 6 1572.5 7 2854.5 8 4113.5 9 4581.5 10 4010.5 11 3420.0 12 3456.5 13 3697.5 14 3912.5 15 4169.0 16 4539.0 17 4961.0 18 5368.5 19 5931.0 20 6628.5 21 7495.5 22 8868.0 23 10582.0 24 12505.5 25 15146.0 26 19259.5 27 23975.0 28 27698.0 29 31834.5 30 37597.5 31 44451.5 32 52913.5 33 59818.5 34 66767.5 35 74646.0 36 83739.5 37 92307.0 38 96291.0 39 98747.0 40 101503.0 41 106891.5 42 113026.5 43 118771.5 44 125062.0 45 130235.0 46 132977.5 47 136548.0 48 146634.0 49 147873.5 50 138499.0 51 131912.5 52 125553.0 53 120023.5 54 113909.5 55 104195.5 56 93055.5 57 81543.0 58 71748.0 59 63297.5 60 54100.0 61 44717.5 62 35136.0 63 25763.5 64 18344.0 65 13570.0 66 9767.5 67 7271.0 68 5165.5 69 3418.0 70 2674.0 71 2130.0 72 1739.0 73 1582.0 74 1284.5 75 1000.0 76 674.5 77 351.0 78 202.0 79 126.5 80 78.0 81 42.5 82 26.0 83 18.0 84 8.5 85 3.0 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022161911811173586 2 0.023893311171421523 3 1.1542662401652908E-4 4 0.0 5 0.0 6 2.885665600413227E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8856656004132275E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.328498400619841E-5 26-27 2.1642492003099206E-4 28-29 1.0099829601446295E-4 30-31 3.7513652805371955E-4 32-33 0.001962252608280995 34-35 0.003736936952535129 36-37 0.10293169196673982 38-39 0.09508268153361583 40-41 0.10394167492688444 42-43 0.10493722955902701 44-45 0.09864647855012618 46-47 0.09174973776513856 48-49 0.09239901252523154 50-51 0.09154774117310964 52-53 0.07199735673031002 54-55 0.0614358206327976 56-57 0.03325729604476244 58-59 0.013620341633950431 60-61 0.010013259633433899 62-63 0.00209210756029959 64-65 0.005410623000774801 66-67 0.15292584849389898 68-69 0.4130830306991535 70-71 0.3931430814002981 72-73 0.18181136115403537 74-75 0.030256203820332686 76-77 0.0307179103163988 78-79 0.005396194672772735 80-81 0.0015149744402169444 82-83 4.328498400619841E-5 84-85 4.6170649606611634E-4 86-87 9.955546321425634E-4 88-89 1.7313993602479364E-4 90-91 7.214164001033068E-4 92-93 0.0028135239604028966 94-95 0.07717712648305176 96-97 0.017414991898493826 98-99 0.019117534602737633 100-101 0.010171971241456626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3465405.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.94779458872497 #Duplication Level Percentage of deduplicated Percentage of total 1 69.88527233180194 30.71303593215136 2 14.388641538596737 12.646981254980899 3 5.930729812315759 7.819274866586411 4 3.022287767395461 5.312915279580476 5 1.8032569948971011 3.962458400120964 6 1.150552929553988 3.033855828689665 7 0.749172376983565 2.304713161465438 8 0.536506238212411 1.8862612762022866 9 0.39227231664842294 1.5515552875417353 >10 1.8994283899453772 14.818052824445877 >50 0.14276838072137288 4.355627688052947 >100 0.09134297868294074 7.572404720447298 >500 0.005793043168147127 1.7291100514791231 >1k 0.0017774109690721024 1.3062567610004363 >5k 6.583003589155935E-5 0.22545810587753096 >10k+ 1.316600717831187E-4 0.7620385613774112 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14624 0.42199973740443036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11716 0.3380845817444137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7793 0.22487992024020279 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.771331200826455E-5 0.0 0.0 2.8856656004132275E-5 0.0 3 5.771331200826455E-5 0.0 0.0 2.8856656004132275E-5 0.0 4 5.771331200826455E-5 0.0 0.0 2.8856656004132275E-5 0.0 5 5.771331200826455E-5 0.0 0.0 2.8856656004132275E-5 0.0 6 8.656996801239682E-5 0.0 0.0 2.8856656004132275E-5 0.0 7 1.154266240165291E-4 0.0 0.0 2.8856656004132275E-5 0.0 8 1.154266240165291E-4 0.0 0.0 2.8856656004132275E-5 0.0 9 1.154266240165291E-4 0.0 0.0 2.8856656004132275E-5 0.0 10-11 1.154266240165291E-4 0.0 0.0 5.771331200826455E-5 0.0 12-13 1.4428328002066138E-4 0.0 0.0 5.771331200826455E-5 0.0 14-15 2.452815760351243E-4 0.0 0.0 5.771331200826455E-5 0.0 16-17 2.5970990403719043E-4 0.0 0.0 1.2985495201859524E-4 0.0 18-19 3.17423216045455E-4 0.0 0.0 1.587116080227275E-4 0.0 20-21 4.328498400619841E-4 0.0 0.0 4.039931840578518E-4 0.0 22-23 4.905631520702486E-4 0.0 0.0 0.0011398379121632248 0.0 24-25 6.3484643209091E-4 0.0 0.0 0.002308532480330582 0.0 26-27 6.925597440991746E-4 0.0 0.0 0.00526633972075414 0.0 28-29 8.224146961177698E-4 0.0 0.0 0.013836766553981424 0.0 30-31 8.656996801239682E-4 0.0 0.0 0.047353772502781055 0.0 32-33 8.945563361281005E-4 0.0 0.0 0.1190914193290539 0.0 34-35 0.0010099829601446296 0.0 0.0 0.23228165250526273 0.0 36-37 0.0011254095841611586 0.0 0.0 0.4257799593409717 0.0 38-39 0.0012408362081776877 0.0 0.0 0.7262210333279948 0.0 40-41 0.0012408362081776877 0.0 0.0 1.063973763528361 0.0 42-43 0.0013129778481880184 0.0 0.0 1.3843115018302332 0.0 44-45 0.0014572611282086798 0.0 0.0 1.7528109990030025 0.0 46-47 0.0017602560162520687 0.0 0.0 2.185271274208931 0.0 48-49 0.0018468259842644656 0.0 0.0 2.6533406629239584 0.0 50-51 0.0018468259842644656 0.0 0.0 3.1671334230775336 0.0 52-53 0.0018468259842644656 0.0 0.0 3.6364436480007387 0.0 54-55 0.0018468259842644656 0.0 0.0 4.1214951787741985 0.0 56-57 0.0018756826402685977 0.0 0.0 4.665472001108096 0.0 58-59 0.0019333959522768623 0.0 0.0 5.238651759318175 0.0 60-61 0.0019333959522768623 0.0 0.0 5.838163216132025 0.0 62-63 0.0019622526082809944 0.0 0.0 6.393942410771613 0.0 64-65 0.0019622526082809944 0.0 0.0 6.935884838857218 0.0 66-67 0.001991109264285127 0.0 0.0 7.515095638172161 0.0 68-69 0.002019965920289259 0.0 0.0 8.157603512432168 0.0 70-71 0.002077679232297524 0.0 0.0 8.906246167475373 0.0 72-73 0.002120964216303722 0.0 0.0 9.653013139878311 0.0 74-75 0.0021642492003099206 0.0 0.0 10.364618276940213 0.0 76-77 0.002193105856314053 0.0 0.0 11.109264285126846 0.0 78-79 0.002193105856314053 0.0 0.0 11.943005218726238 0.0 80-81 0.0025682423843677725 0.0 0.0 12.847329532911738 0.0 82-83 0.002625955696376037 0.0 0.0 13.734859273302831 0.0 84-85 0.0027269539923904995 0.0 0.0 14.566681239277948 0.0 86-87 0.0028279522884049626 0.0 0.0 15.40460638799794 0.0 88-89 0.0028279522884049626 0.0 0.0 16.287735488348403 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 23125 0.0 29.920124 1 GTATCAA 19875 0.0 29.607529 1 TACATGG 23480 0.0 29.239101 2 GGTATCA 13790 0.0 29.113451 1 ACATGGG 23970 0.0 27.804438 3 GTCGGGA 980 0.0 25.659216 2 CATGGGA 16835 0.0 25.049236 4 ATCAACG 24280 0.0 24.050007 3 TCAACGC 24305 0.0 24.02527 4 TATCAAC 24615 0.0 23.804588 2 CAACGCA 24685 0.0 23.655424 5 AACGCAG 25185 0.0 23.242298 6 ATGGGAG 5915 0.0 22.935972 5 CATGGGG 7315 0.0 22.177757 4 AGTACAT 17165 0.0 22.002035 2 ATGGGAT 6640 0.0 21.14606 5 GAGTACA 20440 0.0 20.454206 1 GAGTACT 12850 0.0 20.432741 12-13 ACGCAGA 28975 0.0 20.153034 7 CGCAGAG 29175 0.0 19.933586 8 >>END_MODULE