FastQCFastQC Report
Fri 27 May 2016
SRR939835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824180
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136240.7468561216546612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98530.5401331009001304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66510.3646021774167023No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC25290.13863763444396934No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA23510.12887982545582125No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA23170.12701597430078174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.12317863368746505No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21870.11988948459033648No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21370.11714852700939601No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT20740.113694920457411No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG20580.11281781403151006No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC20200.11073468626999529No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC19460.10667806905020338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19310.10585578177592124No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT19150.10497867535002028No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19020.10426602637897575No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.10179916455612932No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA18520.10152506879803529No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT18510.10147024964641646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT15050.034.9943924
CATATAG11300.033.1711583
CATTCCG5850.032.4417429
CCTTTAG6850.031.1663681
GACATAT10600.030.8821471
GTACATG122400.030.7366161
GTATCAA167650.030.6187881
GGTATCA110850.030.2585411
CATGGGG40450.030.1457864
TTAGGCA7400.029.49434
TACATGG131350.029.4007632
ACATGGG125950.029.1201293
TAGGCAT7050.027.5934965
CGCGTAC350.00814238327.31267490-91
ACGGCGT350.00833874427.17957794-95
ACACGGG700.00843835927.1127723
TATCAAC200850.026.5024052
GGAACGC909.00914E-426.3589158
GGCATTC7400.026.2883997
TCAACGC204900.025.9582634
Produced by FastQC (version 0.11.5)