##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939835_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1824180 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35245315703494 31.0 31.0 34.0 30.0 34.0 2 31.555015404181606 31.0 31.0 34.0 30.0 34.0 3 31.592171825148835 31.0 31.0 34.0 30.0 34.0 4 35.42747481059983 37.0 35.0 37.0 33.0 37.0 5 35.19113135765111 37.0 35.0 37.0 32.0 37.0 6 35.23077656810183 37.0 35.0 37.0 32.0 37.0 7 35.15200035084257 37.0 35.0 37.0 32.0 37.0 8 35.107360019296344 37.0 35.0 37.0 32.0 37.0 9 36.637618546415375 39.0 35.0 39.0 32.0 39.0 10-11 36.51432671117982 38.0 35.0 39.0 32.0 39.0 12-13 36.49217429201066 38.0 35.0 39.0 32.0 39.0 14-15 37.42564741418062 39.0 36.0 41.0 32.0 41.0 16-17 37.36889369470118 39.0 36.0 41.0 32.0 41.0 18-19 37.48160406319552 39.0 36.0 41.0 32.0 41.0 20-21 37.47023128200068 39.0 36.0 41.0 32.0 41.0 22-23 37.318549430429016 39.0 36.0 40.5 31.5 41.0 24-25 37.155694065278645 39.0 36.0 40.0 31.0 41.0 26-27 36.84467870495236 39.0 35.5 40.0 31.0 41.0 28-29 36.634967766338846 38.5 35.0 40.0 30.0 41.0 30-31 36.30678852964071 38.0 35.0 40.0 30.0 41.0 32-33 36.144389808023334 38.0 35.0 40.0 30.0 41.0 34-35 36.09004182701268 38.0 35.0 40.0 30.0 41.0 36-37 35.94248950213246 38.0 35.0 40.0 30.0 41.0 38-39 35.75148505081735 38.0 35.0 40.0 29.0 41.0 40-41 35.55496277779605 38.0 35.0 40.0 28.5 41.0 42-43 35.25246275038648 38.0 34.0 40.0 27.0 41.0 44-45 34.85323487813703 38.0 34.0 40.0 25.5 41.0 46-47 34.37002625837363 38.0 33.5 40.0 23.5 41.0 48-49 33.94920457411001 38.0 33.0 40.0 21.0 41.0 50-51 33.69447751866592 38.0 33.0 40.0 19.0 41.0 52-53 33.45923428609019 38.0 33.0 40.0 16.5 41.0 54-55 33.1786216272517 38.0 33.0 40.0 12.0 41.0 56-57 31.899559528116743 37.5 30.5 40.0 5.0 41.0 58-59 28.692737010602023 34.5 22.0 39.0 2.0 40.0 60-61 28.639716749443586 34.5 21.5 39.0 2.0 40.0 62-63 30.541441085857755 35.5 28.5 39.0 2.0 40.0 64-65 30.17684521264349 36.0 28.0 39.0 2.0 40.0 66-67 24.81739384271289 28.5 16.5 36.0 2.0 39.0 68-69 17.235218015765987 13.0 7.0 29.0 2.0 36.5 70-71 16.21276765450778 13.0 6.5 25.5 2.0 33.5 72-73 18.2400796522273 16.5 7.0 30.0 2.0 35.0 74-75 18.857000679757483 17.5 7.5 31.0 2.0 35.5 76-77 19.705572366762052 22.5 7.5 31.5 2.0 35.0 78-79 22.602222642502383 27.5 8.0 34.0 2.0 36.0 80-81 25.034444791632403 31.5 16.5 35.0 2.0 37.0 82-83 25.92436464603274 32.5 22.0 35.0 2.0 36.5 84-85 25.863765088971483 33.0 21.0 35.0 2.0 36.0 86-87 25.55741894988433 33.0 20.0 35.0 2.0 36.0 88-89 25.08787838919405 32.5 15.5 35.0 2.0 35.5 90-91 23.42205703384534 31.5 2.0 34.5 2.0 35.0 92-93 21.330852218531067 30.0 2.0 34.0 2.0 35.0 94-95 17.173035555701738 18.5 2.0 33.0 2.0 35.0 96-97 15.919367880362682 10.0 2.0 31.5 2.0 34.5 98-99 14.86750896293129 2.0 2.0 31.5 2.0 34.0 100-101 14.204555745595282 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.420191575579981 1101 2 0.4030778520253335 1101 3 0.34945538749494887 1101 4 0.37045957434686727 1101 5 0.3693872153685547 1101 6 0.47025178298422077 1101 7 0.4000373846497709 1101 8 0.43600760738296174 1101 9 0.452705170653644 1101 10-11 0.4127155337713688 1101 12-13 0.4757076522111703 1101 14-15 0.5660153703615975 1101 16-17 0.47099784954412627 1101 18-19 0.4732991104017472 1101 20-21 0.5221215032132989 1101 22-23 0.5593562911508911 1101 24-25 0.541016837848801 1101 26-27 0.7430660278558605 1101 28-29 0.7362656190082006 1101 30-31 0.5236920984850784 1101 32-33 0.5208872851951654 1101 34-35 0.7883406332905452 1101 36-37 0.7167865429729616 1101 38-39 0.8047744514441391 1101 40-41 0.6307311485773042 1101 42-43 0.6536344309198228 1101 44-45 0.3907531499132233 1101 46-47 0.2049863684163924 1101 48-49 0.24372133828246945 1101 50-51 0.4246079934329714 1101 52-53 0.409466980117827 1101 54-55 0.3244154865018771 1101 56-57 1.0789640465587595 1101 58-59 -7.18497196265017 1101 60-61 -8.373144941049123 1101 62-63 -2.0141955082216683 1101 64-65 0.10005093972473489 1101 66-67 -1.4318822598707186 1101 68-69 -3.5775390754445073 1101 70-71 -4.543916283779833 1101 72-73 -6.587648730211889 1101 74-75 -7.2605382939600105 1101 76-77 -8.177313908383283 1101 78-79 -11.380213423942793 1101 80-81 -7.069303829119573 1101 82-83 -3.3330847075527466 1101 84-85 -2.503455165212131 1101 86-87 -2.1777754230760316 1101 88-89 -2.3978123699645337 1101 90-91 -7.707369765626879 1101 92-93 -13.716121415385732 1101 94-95 -11.528173277751012 1101 96-97 -11.670648742850462 1101 98-99 -11.366064848617228 1101 100-101 -11.250187170811573 1102 1 0.38919613348076965 1102 2 0.49055620662721466 1102 3 0.4744129776489068 1102 4 0.40604002339654954 1102 5 0.44092308201347663 1102 6 0.4890643615073742 1102 7 0.5280498390267141 1102 8 0.514172737205655 1102 9 0.6244132065909938 1102 10-11 0.7293021544418963 1102 12-13 0.6822945394255981 1102 14-15 0.9014062118226391 1102 16-17 0.8777065789473895 1102 18-19 0.791792197653308 1102 20-21 0.8779639423339916 1102 22-23 0.8235505447353404 1102 24-25 0.7662288193136106 1102 26-27 0.9491458039951226 1102 28-29 0.9886474598688366 1102 30-31 0.9195100246018129 1102 32-33 0.7222650461269637 1102 34-35 0.7724022112575781 1102 36-37 0.9052773578247937 1102 38-39 0.7732538274964824 1102 40-41 0.8149559056270519 1102 42-43 0.8520386461083973 1102 44-45 0.5620473651424547 1102 46-47 0.5459262925823865 1102 48-49 0.7497239973967567 1102 50-51 0.7744138907394742 1102 52-53 0.6464019239787149 1102 54-55 0.6396400226364207 1102 56-57 1.3808570955906418 1102 58-59 -7.808756081964258 1102 60-61 -9.167476500886647 1102 62-63 -2.234213949445799 1102 64-65 0.2646926256555986 1102 66-67 -1.1397804075712799 1102 68-69 -3.533889574355971 1102 70-71 -4.176236132852733 1102 72-73 -6.6440749456633945 1102 74-75 -7.539551936942068 1102 76-77 -8.266658070326326 1102 78-79 -11.455771844719845 1102 80-81 -7.27303847065642 1102 82-83 -3.5566662534983635 1102 84-85 -2.6567163299005756 1102 86-87 -2.3907080882124063 1102 88-89 -2.60065770357134 1102 90-91 -7.91246807289237 1102 92-93 -13.62095485029187 1102 94-95 -11.71781610439944 1102 96-97 -11.683154169729562 1102 98-99 -11.328561217521221 1102 100-101 -11.036133214193175 1103 1 0.3589390735001068 1103 2 0.3084151690400816 1103 3 0.36021820354664413 1103 4 0.148977995473075 1103 5 0.2152704297279442 1103 6 0.24098834844935624 1103 7 0.2486586243514779 1103 8 0.30265817913040394 1103 9 0.3477632748232864 1103 10-11 0.30866584807566966 1103 12-13 0.3841795114161357 1103 14-15 0.6388503581468399 1103 16-17 0.5000011962294337 1103 18-19 0.4381182994747306 1103 20-21 0.43501058655098035 1103 22-23 0.42992858265179024 1103 24-25 0.4566131546058827 1103 26-27 0.35094789114879177 1103 28-29 0.4982716353343406 1103 30-31 0.47633015397703105 1103 32-33 0.6072206585768853 1103 34-35 0.74329337241155 1103 36-37 0.37134707233712305 1103 38-39 0.3872541117733377 1103 40-41 0.5261105844088192 1103 42-43 0.5599296978801291 1103 44-45 0.6217184521387793 1103 46-47 0.6605050744151058 1103 48-49 0.7545321368398206 1103 50-51 0.5620529363413453 1103 52-53 0.5931874604869307 1103 54-55 0.6359135683350985 1103 56-57 1.3030104225950083 1103 58-59 -5.927830503422836 1103 60-61 -8.148653687844305 1103 62-63 -1.7753095033206492 1103 64-65 0.29059135875180075 1103 66-67 -1.4571342813699033 1103 68-69 -4.173773091036178 1103 70-71 -4.543572283217642 1103 72-73 -7.378162022121586 1103 74-75 -7.922966254083903 1103 76-77 -8.554434867368442 1103 78-79 -11.541992108980338 1103 80-81 -7.629536419593045 1103 82-83 -3.744287224707435 1103 84-85 -2.8504521782067016 1103 86-87 -2.668286599221698 1103 88-89 -2.748068415193522 1103 90-91 -8.082297319599641 1103 92-93 -13.545940124633661 1103 94-95 -11.896884641444046 1103 96-97 -12.01747706144523 1103 98-99 -11.595810564006161 1103 100-101 -11.254717550538322 1104 1 0.24006690602950442 1104 2 0.2305789261406197 1104 3 0.2371475648586383 1104 4 0.2133093854887207 1104 5 0.15979512845468236 1104 6 0.19285348584157447 1104 7 0.2617707313042814 1104 8 0.2611739290537969 1104 9 0.19098952648268153 1104 10-11 0.32536517389051767 1104 12-13 0.1458524516887536 1104 14-15 0.17480112054612817 1104 16-17 0.2978189355572951 1104 18-19 0.32853173281194614 1104 20-21 0.36018252663718897 1104 22-23 0.35202962828557105 1104 24-25 0.3741988516696537 1104 26-27 0.4000255161401256 1104 28-29 0.24214029834062245 1104 30-31 0.23195156067146883 1104 32-33 0.35295738551049993 1104 34-35 0.17892902813494516 1104 36-37 0.2736023980162159 1104 38-39 0.35315161615206847 1104 40-41 0.32987915007707613 1104 42-43 0.2731512670833425 1104 44-45 0.054921271843063835 1104 46-47 0.06510863711501713 1104 48-49 0.09668663723601156 1104 50-51 -0.21862219317628018 1104 52-53 -0.06527507076427241 1104 54-55 0.022669816487564276 1104 56-57 0.8016200724879639 1104 58-59 -6.498855420287104 1104 60-61 -8.180178373290968 1104 62-63 -2.323081623130541 1104 64-65 -0.24939076725053866 1104 66-67 -1.6460617972432807 1104 68-69 -4.187942931394918 1104 70-71 -4.457716965211484 1104 72-73 -7.495661915143202 1104 74-75 -8.400593999800957 1104 76-77 -8.84760876110128 1104 78-79 -11.71158986041766 1104 80-81 -7.914397600998381 1104 82-83 -4.2853064262207745 1104 84-85 -3.4236617904235835 1104 86-87 -3.2544851088037667 1104 88-89 -3.540086204592189 1104 90-91 -8.671438472536433 1104 92-93 -14.536221445814249 1104 94-95 -12.025643456094036 1104 96-97 -11.86455342827239 1104 98-99 -11.246000223155812 1104 100-101 -10.891373214416664 1105 1 0.14280069012462704 1105 2 0.08258402176782553 1105 3 0.08967671507630826 1105 4 0.1537375947298898 1105 5 0.06722524791820206 1105 6 0.0955364973643924 1105 7 0.16758658913495594 1105 8 0.15456300846031468 1105 9 0.03590840390366168 1105 10-11 0.12281348218157717 1105 12-13 0.1386863751150429 1105 14-15 0.0923080647797292 1105 16-17 0.24908173107471754 1105 18-19 0.27196611206877463 1105 20-21 0.29134793066566544 1105 22-23 0.32519887521096535 1105 24-25 0.1579440509188359 1105 26-27 0.32173676104022775 1105 28-29 0.2958205571735917 1105 30-31 0.2722623041222505 1105 32-33 0.04963349145321416 1105 34-35 0.20360500854949493 1105 36-37 0.05672365593093076 1105 38-39 0.15068297204255288 1105 40-41 0.15121608120420404 1105 42-43 0.10627355830919782 1105 44-45 -0.00921239626606507 1105 46-47 0.09126289031298285 1105 48-49 0.07097913164655978 1105 50-51 0.06205522009638287 1105 52-53 0.08200340454813215 1105 54-55 0.0698180180541641 1105 56-57 0.7031406727575416 1105 58-59 -6.501699194612971 1105 60-61 -8.34233895290453 1105 62-63 -2.181839215734989 1105 64-65 -0.1535523066953388 1105 66-67 -1.336612154067307 1105 68-69 -3.8100516056209983 1105 70-71 -4.741864262822187 1105 72-73 -5.7760507667109895 1105 74-75 -6.027434090946654 1105 76-77 -8.12383209306282 1105 78-79 -11.569673376961736 1105 80-81 -7.575171757708052 1105 82-83 -3.4342560590636353 1105 84-85 -2.456632070098813 1105 86-87 -2.205167974387333 1105 88-89 -2.3011999762314446 1105 90-91 -7.633917669176563 1105 92-93 -10.450752326381597 1105 94-95 -11.029688422733395 1105 96-97 -11.493938271358353 1105 98-99 -10.962235888515064 1105 100-101 -10.510464620099977 1106 1 0.05344140828261601 1106 2 0.10630121325143094 1106 3 0.14682521632852286 1106 4 0.06183808487722331 1106 5 0.07992665313097547 1106 6 0.03537260227740546 1106 7 0.06237978461187055 1106 8 0.05624239615399773 1106 9 -1.1855221112000436E-4 1106 10-11 0.015297933891389448 1106 12-13 0.09937860050591496 1106 14-15 0.24133274184860198 1106 16-17 0.06508450464308879 1106 18-19 0.15985017903215493 1106 20-21 0.10232593170774607 1106 22-23 0.19065363038256322 1106 24-25 0.1442119707292946 1106 26-27 0.18498472627251772 1106 28-29 0.2449021109552234 1106 30-31 0.21465547467416712 1106 32-33 0.25304450326209604 1106 34-35 0.2588915597922252 1106 36-37 0.3368103100555544 1106 38-39 0.3155383341642519 1106 40-41 0.3095466635077244 1106 42-43 0.2935264130826667 1106 44-45 0.19143260421784447 1106 46-47 0.14823002246715333 1106 48-49 0.21726762783423226 1106 50-51 0.2079266203904382 1106 52-53 0.17697279851283554 1106 54-55 -0.1101652238654296 1106 56-57 0.6167641701318018 1106 58-59 -6.629089905810524 1106 60-61 -8.394247504696146 1106 62-63 -2.2441169623565287 1106 64-65 -0.26344447958463846 1106 66-67 -1.520756146282224 1106 68-69 -4.035723420359359 1106 70-71 -4.7186491508519595 1106 72-73 -5.63162355763405 1106 74-75 -6.22695110291728 1106 76-77 -8.148436508233655 1106 78-79 -11.576937554045855 1106 80-81 -7.494580239895303 1106 82-83 -3.714247123188482 1106 84-85 -2.6926431166435876 1106 86-87 -2.525910871758679 1106 88-89 -2.5884751044029173 1106 90-91 -7.810550488233048 1106 92-93 -10.592176869279763 1106 94-95 -10.808148721064349 1106 96-97 -11.569788074411134 1106 98-99 -11.106721167522519 1106 100-101 -10.749848628065369 1107 1 0.0989581307776426 1107 2 0.09890945823988417 1107 3 0.03454684558285237 1107 4 -0.03417842484304856 1107 5 0.096170104804429 1107 6 0.03561299106156923 1107 7 0.018796044071670792 1107 8 0.06277878330534037 1107 9 0.03846958389535615 1107 10-11 -0.0035789889187256563 1107 12-13 0.03293644789238925 1107 14-15 0.012706066164021479 1107 16-17 0.11834142582571161 1107 18-19 0.0714228579530456 1107 20-21 0.13089764613867771 1107 22-23 0.16688555620088152 1107 24-25 0.09666154844811103 1107 26-27 0.2456108576806031 1107 28-29 0.08473101070295996 1107 30-31 -0.024648625141871605 1107 32-33 -0.05608213306916099 1107 34-35 -0.05147450616928495 1107 36-37 -0.0741631311192279 1107 38-39 -0.016477529894693532 1107 40-41 -0.08783413927341854 1107 42-43 0.1551345099958965 1107 44-45 0.22443230139734993 1107 46-47 0.0075464218647240955 1107 48-49 -0.005139354913005434 1107 50-51 0.10454116724691431 1107 52-53 0.16498110673342126 1107 54-55 0.2436799961730145 1107 56-57 0.8526110470119193 1107 58-59 -6.20042374340002 1107 60-61 -8.340875591764377 1107 62-63 -2.3198689110131134 1107 64-65 -0.11323577356197845 1107 66-67 -1.5856284347380942 1107 68-69 -3.652705914132863 1107 70-71 -4.373103480317996 1107 72-73 -5.825847956962242 1107 74-75 -6.30834658515559 1107 76-77 -8.511077263070444 1107 78-79 -11.723655019851819 1107 80-81 -7.612440913117297 1107 82-83 -3.610721598888805 1107 84-85 -2.7912502757470747 1107 86-87 -2.764045844324741 1107 88-89 -2.836140918636552 1107 90-91 -7.689062372270138 1107 92-93 -10.683582103138564 1107 94-95 -11.221843011097018 1107 96-97 -11.824981579378324 1107 98-99 -11.027319898344444 1107 100-101 -10.452550934170205 1108 1 -0.031112855283275564 1108 2 -0.03961351543073732 1108 3 0.053223149145061655 1108 4 0.058848420794532785 1108 5 -0.1722904011325923 1108 6 0.08168913974510161 1108 7 0.02852707143357236 1108 8 -0.06676664777364749 1108 9 -0.06750873308966732 1108 10-11 0.024498192289321707 1108 12-13 -0.06284620569563515 1108 14-15 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2312 84-85 0.9045014292769693 2312 86-87 0.5822710320168589 2312 88-89 0.8336210572893279 2312 90-91 2.305902372451758 2312 92-93 3.9428359198486937 2312 94-95 7.751350410634906 2312 96-97 8.27201289118114 2312 98-99 8.728630703508664 2312 100-101 7.98534645191245 2313 1 -0.3555686119449142 2313 2 -0.2062082866725703 2313 3 -0.2699905260686144 2313 4 -0.2999353901657429 2313 5 -0.2983959818264097 2313 6 -0.39470678785670543 2313 7 -0.2993024911606952 2313 8 -0.36314095647149003 2313 9 -0.20418042740842424 2313 10-11 -0.3649153320947889 2313 12-13 -0.4679206149465074 2313 14-15 -0.4122351373006694 2313 16-17 -0.412902563768057 2313 18-19 -0.6411354746210804 2313 20-21 -0.4455513468124863 2313 22-23 -0.42475436943036016 2313 24-25 -0.44369126450636287 2313 26-27 -0.44589207286693977 2313 28-29 -0.5770126060347351 2313 30-31 -0.6276598175727273 2313 32-33 -0.47069302991456397 2313 34-35 -0.407692092153944 2313 36-37 -0.4937578146302215 2313 38-39 -0.8172407882181574 2313 40-41 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2314 4 -0.32428155824110405 2314 5 -0.37225266528353274 2314 6 -0.4178695604645384 2314 7 -0.41786496210940527 2314 8 -0.3176691634969231 2314 9 -0.6474248756473244 2314 10-11 -0.4522743443983117 2314 12-13 -0.5192382392386108 2314 14-15 -0.6550387173683987 2314 16-17 -0.6176418373197592 2314 18-19 -0.7364652127088647 2314 20-21 -0.6216709480153355 2314 22-23 -0.5318184577652616 2314 24-25 -0.5616738953913156 2314 26-27 -0.7969822670481506 2314 28-29 -0.6682822779092916 2314 30-31 -0.6318159309130778 2314 32-33 -0.6678113789474551 2314 34-35 -1.051555273614241 2314 36-37 -0.8230429394203398 2314 38-39 -0.650631038367564 2314 40-41 -0.6916015008918635 2314 42-43 -0.8003524811691989 2314 44-45 -0.6604675956748949 2314 46-47 -0.4408392574314348 2314 48-49 -0.6901844710395437 2314 50-51 -0.5018595918858892 2314 52-53 -0.6903873984546394 2314 54-55 -0.7074532644290272 2314 56-57 0.5418019547351776 2314 58-59 3.6740254956813594 2314 60-61 3.659657716892273 2314 62-63 1.1862423570330662 2314 64-65 1.1151548086021883 2314 66-67 2.027266865760094 2314 68-69 0.4282617583252488 2314 70-71 2.950980560034882 2314 72-73 8.169278397414462 2314 74-75 9.55211135137554 2314 76-77 8.485669581181781 2314 78-79 6.166557399881306 2314 80-81 3.442001350470843 2314 82-83 1.9152650897704184 2314 84-85 1.5744050526314375 2314 86-87 1.6163115942351816 2314 88-89 1.7636408333865603 2314 90-91 2.794610157274242 2314 92-93 4.692126675159809 2314 94-95 8.307495323594615 2314 96-97 8.907639891070803 2314 98-99 9.040263377355839 2314 100-101 8.18296790940233 2315 1 -0.6971290374006642 2315 2 -0.5954001308997476 2315 3 -0.5393736692860962 2315 4 -0.5266957371868131 2315 5 -0.9074346688232069 2315 6 -0.6849101471446417 2315 7 -0.7957576241232047 2315 8 -0.6881432248444099 2315 9 -1.1689239826782583 2315 10-11 -0.9687297287199996 2315 12-13 -0.7938670504570169 2315 14-15 -0.8729882867537668 2315 16-17 -1.0612922120447692 2315 18-19 -0.9208473678175366 2315 20-21 -0.9981847691984171 2315 22-23 -1.0209917110131173 2315 24-25 -1.0760900557738537 2315 26-27 -1.173346445476355 2315 28-29 -1.0186307543419204 2315 30-31 -1.02270365025975 2315 32-33 -1.0894763436254422 2315 34-35 -1.0589757430181166 2315 36-37 -1.0698758604814103 2315 38-39 -1.0808276525600817 2315 40-41 -0.8691749825282145 2315 42-43 -0.9740838383556749 2315 44-45 -0.9048609517090753 2315 46-47 -0.7290428658774388 2315 48-49 -0.4744875929237864 2315 50-51 -0.527714151203071 2315 52-53 -0.43368941174625775 2315 54-55 -0.6015523374201877 2315 56-57 0.6876459973723001 2315 58-59 3.782168476530533 2315 60-61 3.5114803397718255 2315 62-63 1.109402168020324 2315 64-65 0.8948447361641705 2315 66-67 1.89220428892623 2315 68-69 6.680033609042443 2315 70-71 5.808487451364055 2315 72-73 8.942106033110687 2315 74-75 9.761601145768406 2315 76-77 8.387695120211948 2315 78-79 5.827196812879208 2315 80-81 2.972552478555844 2315 82-83 1.473289535157857 2315 84-85 1.046312320698103 2315 86-87 0.9118163603784843 2315 88-89 1.0683028448841831 2315 90-91 2.1902400138356946 2315 92-93 3.940137188389894 2315 94-95 7.561707026836789 2315 96-97 8.19315754862838 2315 98-99 8.302321909666595 2315 100-101 7.554059516643141 2316 1 -0.5222451422787913 2316 2 -0.6013803315742372 2316 3 -0.7489248329177194 2316 4 -0.520398215976762 2316 5 -0.7146356926124113 2316 6 -0.6499722952324731 2316 7 -0.7280693411224846 2316 8 -0.7803070065180435 2316 9 -1.012598419188322 2316 10-11 -0.7563515834954302 2316 12-13 -0.8389717662337546 2316 14-15 -0.9086587124798484 2316 16-17 -1.1151164779642997 2316 18-19 -1.044230017025832 2316 20-21 -0.8647018257921673 2316 22-23 -1.0408898917087157 2316 24-25 -2.335782322500215 2316 26-27 -1.4764096955765993 2316 28-29 -1.1302479452434255 2316 30-31 -0.834717215097676 2316 32-33 -0.7927731871470556 2316 34-35 -1.0854078979197297 2316 36-37 -0.979364328708769 2316 38-39 -1.2423865450376113 2316 40-41 -1.0714177807049694 2316 42-43 -1.1241214125508705 2316 44-45 -1.2152328747453183 2316 46-47 -1.0057429905917914 2316 48-49 -1.1023368668993143 2316 50-51 -1.1507053769212874 2316 52-53 -0.9958371921014049 2316 54-55 -0.9735696166302859 2316 56-57 0.08098909220686323 2316 58-59 3.0380121555792634 2316 60-61 3.0198246091843437 2316 62-63 0.7090669520824733 2316 64-65 0.3759630881144602 2316 66-67 5.027394367616804 2316 68-69 8.347785171661588 2316 70-71 5.422713927212026 2316 72-73 8.561653316330332 2316 74-75 9.35716336273202 2316 76-77 7.9330395175366135 2316 78-79 5.276748382385932 2316 80-81 2.3354967580537362 2316 82-83 1.0252792148609018 2316 84-85 0.7609197041812017 2316 86-87 0.6467022787262557 2316 88-89 0.4824416578593933 2316 90-91 2.017169642472272 2316 92-93 3.6937936003555834 2316 94-95 7.410657839693929 2316 96-97 8.050246311053693 2316 98-99 8.237914757901287 2316 100-101 7.192497057384395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 32.0 5 86.0 6 258.0 7 617.0 8 1353.0 9 2518.0 10 4740.0 11 7519.0 12 11023.0 13 14573.0 14 17962.0 15 24483.0 16 32007.0 17 40228.0 18 37789.0 19 33152.0 20 33655.0 21 33740.0 22 32531.0 23 31156.0 24 31810.0 25 33143.0 26 41215.0 27 58410.0 28 81137.0 29 106662.0 30 128117.0 31 137937.0 32 141226.0 33 147272.0 34 152795.0 35 151740.0 36 133022.0 37 88140.0 38 30671.0 39 1444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.59735996398655 24.585212003972007 15.020756534703247 20.796671497338195 2 14.192062172848061 24.1993754646188 41.110184005491 20.498378357042142 3 16.640142359601626 31.119069926040765 33.348865996788575 18.891921717569033 4 10.272286726090627 20.12197261235185 42.772917146334244 26.832823515223282 5 9.301838260906772 42.21154125705647 37.067416796628365 11.419203685408396 6 23.653338654451485 43.23876288388524 19.986481582389438 13.121416879273843 7 22.960398644870573 37.32531877336666 24.76526439276826 14.949018188994506 8 24.037456870985178 36.46804204414262 22.433391916797593 17.061109168074605 9 26.169950333848636 16.89279566709426 24.37791226742975 32.55934173162736 10-11 21.270241971735246 29.58044162308544 30.814365906873224 18.33495049830609 12-13 22.68920830181232 28.77925972217654 30.544052670240873 17.98747930577026 14-15 20.799956363823746 29.211295300970384 29.659059481425707 20.329688853780162 16-17 17.322660044513153 32.57329320571435 30.83807518994836 19.26597155982414 18-19 17.133261318313423 30.944713351074864 34.48341740415364 17.438607926458065 20-21 17.967634772884256 30.270148779177493 34.12823844138188 17.63397800655637 22-23 18.014231051760245 30.360682608075955 33.42145511956057 18.20363122060323 24-25 17.57504741856615 31.327911719238234 33.9225295749323 17.174511287263318 26-27 17.431682261357647 30.71294832166918 34.75876949541944 17.09659992155373 28-29 16.659731918535073 30.339276385072033 35.1773459046886 17.823645791704287 30-31 17.376450516061897 30.551599062722378 34.70534088155412 17.3666095396616 32-33 17.200583386530855 30.94807437767124 34.370303531483934 17.481038704313974 34-35 17.590665529707177 30.84567255073353 34.1394562493987 17.424205670160596 36-37 17.977508682300062 30.167137189251527 33.76880776604906 18.086546362399357 38-39 16.878772558584327 31.183678639422357 33.80256358515893 18.134985216834384 40-41 16.88843729299824 30.42651684308883 34.85695767184162 17.828088192071313 42-43 16.86480507950852 30.38010222406713 34.27642831017727 18.478664386247072 44-45 16.939715266877325 30.431568773947674 33.90758854213077 18.72112741704423 46-47 17.490275472750348 30.724351499401877 32.94990898549882 18.835464042348956 48-49 16.85357275738053 30.828167494105745 33.031475952628824 19.286783795884897 50-51 16.48190761380208 30.54623299079362 33.11781689949698 19.85404249590732 52-53 16.578122292675115 31.229013091663578 32.89510767887809 19.297756936783212 54-55 16.6558517303761 30.67085776288999 32.54583051994211 20.127459986791795 56-57 17.070844768926445 30.744666424732614 31.939989008654173 20.244499797686764 58-59 16.1155234178458 29.891540753207778 33.23999318383996 20.752942645106465 60-61 17.413532246991732 30.620366141971502 31.274601529793472 20.6915000812433 62-63 16.501569202516205 30.05320213247084 32.158345553469374 21.286883111543574 64-65 16.034434534666335 29.89468333222167 32.371377603718486 21.699504529393504 66-67 17.823748262160567 29.731544986168174 30.046156125743263 22.398550625927992 68-69 19.58905352061577 29.187024984324587 28.721973687842695 22.501947807216943 70-71 18.559612510695572 29.624479650255488 29.441499212436057 22.374408626612883 72-73 18.288364668698538 28.961238647527548 29.708528882655884 23.04186780111803 74-75 18.093099927864692 29.501475697417025 29.20043398115907 23.204990393559218 76-77 16.099338075068417 29.883213847595957 30.8162059356501 23.201242141685526 78-79 16.442488983328424 29.541056884834838 30.031185231467084 23.985268900369654 80-81 16.522720395400444 29.432028488479183 29.84613326784258 24.199117848277798 82-83 16.60541991915813 29.630899607686416 29.627117083114403 24.13656339004105 84-85 16.41867382532087 28.92136681762042 29.443930666891795 25.21602869016692 86-87 16.279650484862074 28.902812139724922 29.980807257389426 24.83673011802358 88-89 16.944800921400805 28.474288981508945 29.697359743753214 24.883550353337036 90-91 16.95808503642146 28.376515225809744 29.600755322571032 25.064644415197762 92-93 16.73647979299877 28.19460948945994 29.788358597118584 25.2805521204227 94-95 16.78450822253062 27.674245289253264 29.39860822569601 26.142638262520112 96-97 16.691152179404963 27.323111603412425 29.755450247492455 26.230285969690154 98-99 16.450967407578027 27.343355495707794 29.57384123707687 26.63183585963731 100-101 15.818651014577787 27.478156435942747 30.173716500549443 26.52947604893002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 942.0 1 662.5 2 375.5 3 396.0 4 462.0 5 621.0 6 949.0 7 1396.5 8 1792.0 9 2038.0 10 2182.5 11 2331.0 12 2559.5 13 2838.0 14 3159.0 15 3461.5 16 3837.5 17 4365.0 18 4884.5 19 5542.0 20 6411.0 21 7291.0 22 8336.5 23 9869.5 24 11655.0 25 14102.5 26 17946.0 27 22460.0 28 26358.5 29 30245.0 30 35350.5 31 41952.0 32 51112.5 33 62837.0 34 75157.5 35 85430.5 36 94007.0 37 99650.0 38 100041.0 39 97796.0 40 93873.0 41 88956.0 42 84658.5 43 81367.0 44 78628.0 45 74857.5 46 69263.0 47 62305.0 48 54849.0 49 47212.5 50 38707.0 51 29826.0 52 21815.0 53 15668.0 54 11434.5 55 8212.5 56 5862.0 57 4184.5 58 2962.0 59 2054.5 60 1371.5 61 925.5 62 663.0 63 497.0 64 379.0 65 287.5 66 239.5 67 210.0 68 162.0 69 114.0 70 83.5 71 63.5 72 52.0 73 36.0 74 20.0 75 15.5 76 10.0 77 3.5 78 1.5 79 2.5 80 3.0 81 3.0 82 2.5 83 1.5 84 1.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022859586225043582 2 0.0057011917683561925 3 0.0036728831584602398 4 0.0 5 0.06616671600390311 6 1.0963830323761909E-4 7 0.0 8 1.0963830323761909E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.015053339034525E-4 16-17 0.0 18-19 0.0012882500630420244 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.222872742821432E-5 28-29 0.001809032003420715 30-31 0.00953853238167286 32-33 1.9186703066583342E-4 34-35 9.045160017103575E-4 36-37 0.02732734708197656 38-39 0.02653246938350382 40-41 0.0287252354482562 42-43 0.02954752272253835 44-45 0.02973938975320418 46-47 0.031219506846912038 48-49 0.03187733666633775 50-51 0.02732734708197656 52-53 0.023243320286375248 54-55 0.017185804032496792 56-57 0.15058820949686982 58-59 0.7552160422765297 60-61 0.305260445789341 62-63 2.7409575809404774E-4 64-65 0.14135118244910042 66-67 1.403945882533522 68-69 2.6920040785448807 70-71 2.1688100954949623 72-73 1.8480084202216887 74-75 1.5486136236555603 76-77 1.107292043548334 78-79 0.4174478395772347 80-81 2.1927660647523819E-4 82-83 8.222872742821432E-5 84-85 5.4819151618809547E-5 86-87 0.002905415035796906 88-89 5.4819151618809547E-5 90-91 0.22094859059961192 92-93 0.17147430626363624 94-95 1.2858928395224156 96-97 1.2671446896687828 98-99 0.9931037507263537 100-101 0.40239998245787145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1824180.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.174670074370322 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0153962445773 20.113439733701764 2 11.701493176675323 6.359684329072948 3 4.593668584859923 3.7449428467370405 4 2.416538079682729 2.626745001501223 5 1.4752134248149422 2.004421905431398 6 0.990484238342152 1.6149649434487177 7 0.709105535621457 1.348879628489592 8 0.5055242496210626 1.09899637584368 9 0.3739461127566308 0.9145676015779252 >10 2.397112659678729 12.827081252987698 >50 0.358607893986394 6.9318841651871494 >100 0.3968951916356557 22.217188181633183 >500 0.0458266543080156 8.642220866757345 >1k 0.019582314836464818 7.840495403062402 >5k 2.018795343965445E-4 0.39131707489640294 >10k+ 4.03759068793089E-4 1.3231706896714344 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13425 0.7359471104825182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10694 0.5862360074115492 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7133 0.3910250084969685 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2838 0.1555767522941815 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2665 0.14609303906412743 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2648 0.14516111348660768 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2608 0.1429683474218553 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2526 0.1384731769891129 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2471 0.1354581236500784 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2413 0.13227861285618744 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2411 0.13216897455294982 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 2363 0.12953765527524697 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2349 0.12877018715258365 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2327 0.1275641658169698 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2310 0.12663224023945005 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2291 0.12559067635869267 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2170 0.11895755901281672 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2162 0.11851900579986624 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2100 0.11512021839950005 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 2092 0.11468166518654957 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2023 0.11089914372485171 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1939 0.10629433498887172 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1921 0.10530759025973314 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1880 0.10306000504336195 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1845 0.10114133473670363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.4819151618809547E-5 0.0 5 0.0 0.0 0.0 5.4819151618809547E-5 0.0 6 0.0 0.0 0.0 5.4819151618809547E-5 0.0 7 0.0 0.0 0.0 5.4819151618809547E-5 0.0 8 0.0 0.0 0.0 5.4819151618809547E-5 0.0 9 0.0 0.0 0.0 5.4819151618809547E-5 0.0 10-11 0.0 0.0 0.0 1.0963830323761909E-4 0.0 12-13 0.0 0.0 0.0 1.0963830323761909E-4 0.0 14-15 0.0 0.0 0.0 1.0963830323761909E-4 0.0 16-17 0.0 0.0 0.0 2.46686182284643E-4 0.0 18-19 0.0 0.0 0.0 3.5632448552226203E-4 0.0 20-21 0.0 0.0 0.0 6.03010667806905E-4 0.0 22-23 0.0 0.0 0.0 0.002302404367990001 0.0 24-25 0.0 0.0 0.0 0.006523479042638336 0.0 26-27 0.0 0.0 0.0 0.01510267627098203 0.0 28-29 0.0 0.0 0.0 0.03842822528478549 0.0 30-31 0.0 0.0 0.0 0.12131478253242553 0.0 32-33 0.0 0.0 0.0 0.32814744159019393 0.0 34-35 0.0 0.0 0.0 0.6752623096404959 0.0 36-37 0.0 0.0 0.0 1.2238649694657324 0.0 38-39 0.0 0.0 0.0 2.0039414970013922 0.0 40-41 0.0 0.0 0.0 2.8815412952669144 0.0 42-43 0.0 0.0 0.0 3.703499654639345 0.0 44-45 0.0 0.0 0.0 4.621583396375358 0.0 46-47 0.0 0.0 0.0 5.640095823877029 0.0 48-49 0.0 5.4819151618809547E-5 0.0 6.59978730169172 0.0 50-51 0.0 5.4819151618809547E-5 0.0 7.564083588242388 0.0 52-53 0.0 5.4819151618809547E-5 0.0 8.412135863785373 0.0 54-55 0.0 5.4819151618809547E-5 0.0 9.278305868938372 0.0 56-57 0.0 5.4819151618809547E-5 0.0 10.029602341874156 0.0 58-59 0.0 1.0963830323761909E-4 0.0 10.52549638741791 0.0 60-61 0.0 1.0963830323761909E-4 0.0 10.888947362650615 0.0 62-63 0.0 1.0963830323761909E-4 0.0 11.176035259678322 0.0 64-65 5.4819151618809547E-5 1.0963830323761909E-4 0.0 11.46761832713877 0.0 66-67 5.4819151618809547E-5 1.0963830323761909E-4 0.0 11.694816301022925 0.0 68-69 5.4819151618809547E-5 1.0963830323761909E-4 0.0 11.94835487725992 0.0 70-71 5.4819151618809547E-5 1.0963830323761909E-4 0.0 12.41478362880856 0.0 72-73 5.4819151618809547E-5 1.0963830323761909E-4 0.0 12.978680831935446 0.0 74-75 5.4819151618809547E-5 1.0963830323761909E-4 0.0 13.622093214485414 0.0 76-77 5.4819151618809547E-5 1.0963830323761909E-4 0.0 14.534092030391736 0.0 78-79 5.4819151618809547E-5 1.0963830323761909E-4 0.0 15.771935883520268 0.0 80-81 5.4819151618809547E-5 1.0963830323761909E-4 0.0 17.14241467399051 0.0 82-83 5.4819151618809547E-5 1.0963830323761909E-4 0.0 18.326755035139076 0.0 84-85 5.4819151618809547E-5 1.3704787904702387E-4 0.0 19.251417075069348 0.0 86-87 5.4819151618809547E-5 1.6445745485642863E-4 0.0 20.076308259053384 0.0 88-89 5.4819151618809547E-5 1.6445745485642863E-4 0.0 20.886644958282623 2.7409575809404773E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 860 0.0 43.277782 9 GGCATTC 910 0.0 40.898758 7 TAGGCAT 930 0.0 39.539738 5 TTAGGCA 960 0.0 39.28628 4 CCTTTAG 965 0.0 38.60584 1 CTTTAGG 995 0.0 36.960796 2 GGTATCA 10165 0.0 33.912766 1 GACATAT 1060 0.0 31.586803 1 GTACATG 12860 0.0 31.132925 1 GTATCAA 15605 0.0 30.914701 1 CATGGGT 1595 0.0 30.148218 4 TACATGG 13310 0.0 29.684912 2 ATGGGCG 255 0.0 29.580257 5 CATATAG 1085 0.0 29.546177 3 ACATGGG 12720 0.0 29.279396 3 GCATTCC 1320 0.0 29.26687 8 AGGCATT 1280 0.0 28.709192 6 CGGGTCT 35 0.0074022827 27.851295 66-67 CGTCGCA 35 0.00740997 27.845371 68-69 GGTCGCG 55 7.969559E-5 26.579674 68-69 >>END_MODULE