FastQCFastQC Report
Fri 27 May 2016
SRR939834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915149
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143150.7474614246724407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100910.5269041729912398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71830.3750622014266253No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC27430.14322645392081765No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA24930.13017263930900416No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA24410.12745744586974694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.1200428791702369No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22820.11915521977663357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22560.11779762305700496No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC22250.11617895004514009No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG22130.11555236694377304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21810.11388147867346092No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT21550.1125238819538323No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT20770.1084510917949465No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC20470.10688463404152888No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT20170.10531817628811127No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA20030.1045871626698497No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA19590.10228969129817053No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19290.10072323354475292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG7900.042.283619
CCTTTAG8550.038.513761
TTAGGCA9450.036.3429764
TAGGCAT9450.036.3429765
AGCGCCA550.00270390234.2166988
GGCATTC9900.033.740587
CATGGGT15450.033.1915444
GTATCAA182450.031.8940581
AAGCGCC600.004141474631.3644857
CTTTAGG10850.031.2183172
GTACATG135400.031.0948411
GGTATCA120250.030.1224041
GACATAT11200.029.8211441
ACATGGG136550.029.4235843
TACATGG143000.029.3121972
CATGGGG42850.028.766034
GGCGCGG400.00681703828.32509292-93
CTAGTCG1555.0384187E-827.3174554
AGGCATT12750.027.3055556
CATATAG12150.026.7178943
Produced by FastQC (version 0.11.5)