##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939834_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1915149 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.415494042500086 31.0 30.0 33.0 27.0 34.0 2 30.48724877281089 31.0 31.0 34.0 26.0 34.0 3 30.402863171481698 31.0 30.0 34.0 26.0 34.0 4 34.111252440410645 35.0 35.0 37.0 30.0 37.0 5 34.05145343782651 35.0 35.0 37.0 30.0 37.0 6 34.09217194066885 36.0 35.0 37.0 30.0 37.0 7 34.11716947349788 36.0 35.0 37.0 30.0 37.0 8 34.00616348910712 36.0 35.0 37.0 29.0 37.0 9 35.29425438960624 38.0 35.0 39.0 29.0 39.0 10-11 35.15141354536905 37.0 35.0 39.0 28.5 39.0 12-13 35.114668623694556 37.0 35.0 39.0 29.0 39.0 14-15 35.71571376430764 38.0 34.0 40.0 27.0 41.0 16-17 35.575283176400376 38.0 34.0 40.0 27.0 41.0 18-19 35.76502559330893 38.5 34.5 40.0 27.0 41.0 20-21 35.949968644737304 39.0 35.0 40.0 27.0 41.0 22-23 35.86249137795545 38.5 34.5 40.0 27.0 41.0 24-25 35.658234946732605 38.0 34.0 40.0 27.0 41.0 26-27 35.51908154404697 38.0 34.0 40.0 27.0 41.0 28-29 35.44683102985721 38.0 34.0 40.0 27.0 41.0 30-31 35.18171118800679 38.0 34.0 40.0 26.0 41.0 32-33 34.832944329657906 38.0 34.0 40.0 25.0 41.0 34-35 34.563847251571545 38.0 33.0 40.0 24.5 41.0 36-37 34.28649572435356 38.0 33.0 40.0 24.0 41.0 38-39 33.98103280736903 38.0 33.0 40.0 23.0 41.0 40-41 33.69735514051388 38.0 33.0 40.0 20.5 41.0 42-43 33.324923282731525 38.0 33.0 40.0 18.0 41.0 44-45 32.79594929689544 37.0 31.5 40.0 13.5 41.0 46-47 32.25817782323986 37.0 31.0 40.0 8.5 41.0 48-49 31.834928248402605 37.0 30.5 40.0 4.5 41.0 50-51 31.12227429823998 36.0 30.0 39.0 2.0 40.5 52-53 31.253487065497254 36.5 30.0 39.0 2.0 40.0 54-55 31.389541753670343 37.0 30.0 39.0 2.0 40.0 56-57 31.245391089675007 37.0 30.0 39.0 2.0 40.0 58-59 30.82023409144667 36.0 29.0 39.0 2.0 40.0 60-61 30.389452465578398 36.0 28.0 39.0 2.0 40.0 62-63 30.030808046789048 35.5 28.0 39.0 2.0 40.0 64-65 29.564875370010377 35.0 27.5 38.5 2.0 40.0 66-67 29.057248287208985 34.5 26.0 38.0 2.0 40.0 68-69 28.51152991229403 34.0 26.0 38.0 2.0 39.5 70-71 27.97058009585677 34.0 25.5 37.0 2.0 39.0 72-73 27.454292068136738 33.5 24.0 37.0 2.0 39.0 74-75 26.806318725070476 33.0 22.0 36.0 2.0 39.0 76-77 26.102740308978568 32.0 20.5 35.5 2.0 38.0 78-79 25.38818963955285 31.5 18.0 35.0 2.0 37.0 80-81 24.61115427572476 31.0 10.5 35.0 2.0 36.5 82-83 23.77739173296699 31.0 4.5 34.0 2.0 36.0 84-85 22.9630028786272 30.0 2.0 34.0 2.0 35.5 86-87 22.139401947315847 29.0 2.0 34.0 2.0 35.0 88-89 21.23026328499767 29.0 2.0 34.0 2.0 35.0 90-91 20.238388501364646 27.5 2.0 33.5 2.0 35.0 92-93 19.224939678322677 26.0 2.0 33.0 2.0 34.5 94-95 17.87196192045632 23.5 2.0 32.0 2.0 34.0 96-97 15.255591340412678 4.5 2.0 31.0 2.0 34.0 98-99 13.877798280969262 2.0 2.0 31.0 2.0 34.0 100-101 12.595904809495241 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6827651788975793 1101 2 0.659858581344487 1101 3 0.7722448353523568 1101 4 0.7002129848066332 1101 5 0.8961450698753382 1101 6 0.920485588563686 1101 7 0.7694964076493065 1101 8 0.6467611449156792 1101 9 0.7497938117785807 1101 10-11 0.818315149670255 1101 12-13 0.626469807826247 1101 14-15 0.7477200259663377 1101 16-17 0.7490544121849254 1101 18-19 0.6698826148700974 1101 20-21 0.7098980773422099 1101 22-23 0.637076490880709 1101 24-25 0.7034193467274221 1101 26-27 0.7835807722203683 1101 28-29 1.1210872088376505 1101 30-31 0.9744986903808481 1101 32-33 1.0495003830291054 1101 34-35 0.9426742246463888 1101 36-37 0.8411686478898375 1101 38-39 0.6896681052323501 1101 40-41 0.9562581367130818 1101 42-43 0.6022645854976929 1101 44-45 0.8522143447240325 1101 46-47 0.7593174938875151 1101 48-49 0.5899376775437162 1101 50-51 0.8220104104082502 1101 52-53 1.0479906408406983 1101 54-55 0.9652492273312419 1101 56-57 0.4724581681957716 1101 58-59 0.31845841840398137 1101 60-61 0.53226571249591 1101 62-63 0.5460922956108298 1101 64-65 0.3097345805455092 1101 66-67 0.362767651137343 1101 68-69 0.3480189103966822 1101 70-71 0.4440807372805615 1101 72-73 0.11432659886887464 1101 74-75 -0.006069210907256206 1101 76-77 -0.3999614768572961 1101 78-79 -0.11607495265876366 1101 80-81 -0.26636111602034873 1101 82-83 -0.45919012092740985 1101 84-85 -0.3505797086436431 1101 86-87 -0.41831579344502856 1101 88-89 -0.4554442282810065 1101 90-91 -0.6319157883343358 1101 92-93 -0.691455404126927 1101 94-95 -0.37040672375497863 1101 96-97 1.2185730083247357 1101 98-99 1.501118196928827 1101 100-101 0.7527057372234331 1102 1 0.6808650676082024 1102 2 0.6821004729042777 1102 3 0.6932054005505499 1102 4 0.8203645541467068 1102 5 0.865116412360905 1102 6 0.6339379169492716 1102 7 0.6076158169564536 1102 8 0.5912663067847816 1102 9 0.7736770718181063 1102 10-11 0.6563478951212716 1102 12-13 0.7726989560529134 1102 14-15 0.9135394514574031 1102 16-17 0.745526918513626 1102 18-19 0.7819422276150263 1102 20-21 0.5845289442604908 1102 22-23 0.5597051479955368 1102 24-25 0.7121396787174277 1102 26-27 0.9118570156344177 1102 28-29 0.947397856054323 1102 30-31 0.8731464345231643 1102 32-33 0.8709049069323456 1102 34-35 0.767792806775752 1102 36-37 0.6958173770530536 1102 38-39 0.6583232620065118 1102 40-41 0.773672127206531 1102 42-43 0.8220973217578518 1102 44-45 0.8892957358212854 1102 46-47 0.7600739919284649 1102 48-49 0.6499572680707217 1102 50-51 0.6079469394983334 1102 52-53 0.6610038057547669 1102 54-55 0.4024816183345834 1102 56-57 0.43098539213517384 1102 58-59 0.30443310956097136 1102 60-61 0.5596557368117416 1102 62-63 0.566967795364075 1102 64-65 0.44475711814313357 1102 66-67 0.2695832764058288 1102 68-69 0.274302934077145 1102 70-71 0.5223494381918563 1102 72-73 0.5004727695751932 1102 74-75 0.1918715359903267 1102 76-77 -0.07839186704944368 1102 78-79 0.0899205378236978 1102 80-81 0.1488796346734631 1102 82-83 -0.12477652803874406 1102 84-85 -0.2570304466101021 1102 86-87 -0.3061959905291296 1102 88-89 -0.5466535661991152 1102 90-91 -0.40219238339884456 1102 92-93 -0.6577893681666716 1102 94-95 -0.34744230039180124 1102 96-97 1.1180419362104175 1102 98-99 1.1446173088116147 1102 100-101 0.6493082643115073 1103 1 0.816979887269472 1103 2 0.7596303278932766 1103 3 0.847589709427762 1103 4 1.0105527377477088 1103 5 0.9639107145209636 1103 6 1.0159078278179479 1103 7 0.8627358683350934 1103 8 0.8779761624322262 1103 9 1.1128802606744017 1103 10-11 0.9282013834488012 1103 12-13 0.8600122994136612 1103 14-15 0.9796527183595032 1103 16-17 1.2292656972488842 1103 18-19 1.0797457121835023 1103 20-21 1.0433536775519912 1103 22-23 1.021732569919024 1103 24-25 1.097666194291449 1103 26-27 1.0764161966424055 1103 28-29 1.1093135382831392 1103 30-31 1.0958236468487357 1103 32-33 1.0194775478071634 1103 34-35 1.1176963981209909 1103 36-37 0.9700270300064702 1103 38-39 0.9156185275160453 1103 40-41 0.9803178381761342 1103 42-43 0.972273825268509 1103 44-45 1.0330035969594036 1103 46-47 0.934801902823672 1103 48-49 0.832405733418895 1103 50-51 1.0413019999945234 1103 52-53 0.9737050563646967 1103 54-55 0.9261861331784189 1103 56-57 0.991806870528972 1103 58-59 0.7579632289121356 1103 60-61 0.883481327560439 1103 62-63 0.7008195668116848 1103 64-65 0.7165715418016809 1103 66-67 0.728237378171503 1103 68-69 0.4972870128904816 1103 70-71 0.7296022485139702 1103 72-73 0.4685456332905993 1103 74-75 0.4598391953582066 1103 76-77 0.42809495636400285 1103 78-79 0.32569387331254873 1103 80-81 0.3077447102206037 1103 82-83 0.23896222121374677 1103 84-85 0.2157472528199449 1103 86-87 0.11172889425466792 1103 88-89 0.17206312748523445 1103 90-91 -0.12544807222197463 1103 92-93 -0.20897022677370103 1103 94-95 0.1527918419323342 1103 96-97 1.8227890796986301 1103 98-99 2.0619045058700216 1103 100-101 1.633967151130598 1104 1 0.5503333229982807 1104 2 0.5279296844882744 1104 3 0.5934967141098717 1104 4 0.829863888175872 1104 5 0.8258002787305472 1104 6 1.0181242252119844 1104 7 0.7575780396633363 1104 8 0.7986763033575599 1104 9 1.1118695257064388 1104 10-11 0.9003889652702028 1104 12-13 0.8189724889697843 1104 14-15 1.0169073263483526 1104 16-17 1.1455407442240002 1104 18-19 0.9199393690033659 1104 20-21 0.877256231149822 1104 22-23 0.793094092490179 1104 24-25 0.9518390376718102 1104 26-27 1.0719475448280846 1104 28-29 1.1434561655009787 1104 30-31 0.9831287434557439 1104 32-33 1.0646052998940903 1104 34-35 0.9729364091716874 1104 36-37 0.9417115305301209 1104 38-39 0.7740605228801414 1104 40-41 0.914465567199052 1104 42-43 0.9280064011602676 1104 44-45 1.0603214769572489 1104 46-47 1.128746782151545 1104 48-49 0.9996917560131493 1104 50-51 1.101581270879194 1104 52-53 1.2464588663542209 1104 54-55 1.1750922532267793 1104 56-57 1.1455974154402604 1104 58-59 1.1185556832461678 1104 60-61 1.4687456058764212 1104 62-63 1.39069996798683 1104 64-65 1.345157966379677 1104 66-67 1.2834586424950594 1104 68-69 1.035008123197283 1104 70-71 1.3289618275861734 1104 72-73 1.4058148488062479 1104 74-75 1.2694481323730535 1104 76-77 0.8556327193476143 1104 78-79 0.8998333287477998 1104 80-81 1.0384041762830094 1104 82-83 0.945368018789523 1104 84-85 0.822287020909215 1104 86-87 0.7071940152348297 1104 88-89 0.7445446165962473 1104 90-91 0.5208629136007836 1104 92-93 0.6362189876253268 1104 94-95 0.7522722278983274 1104 96-97 2.242811426567471 1104 98-99 2.214237306241202 1104 100-101 2.1372957179661682 1105 1 0.6055091042482807 1105 2 0.6705078094882744 1105 3 0.6223053078598717 1105 4 0.615020138175872 1105 5 0.8375190287305472 1105 6 0.8218351627119844 1105 7 0.6950780396633363 1105 8 0.5682075533575599 1105 9 1.0327679632064388 1105 10-11 0.9177229496452028 1105 12-13 0.7154568639697843 1105 14-15 0.7544561544733526 1105 16-17 0.7793298067240002 1105 18-19 0.9287284315033659 1105 20-21 0.788877324899822 1105 22-23 0.672000342490179 1105 24-25 0.8395343501718102 1105 26-27 0.8072991073280846 1105 28-29 0.8648917123759787 1105 30-31 0.7712146809557439 1105 32-33 0.9474177998940903 1105 34-35 0.9973504716716874 1105 36-37 0.7576294992801209 1105 38-39 0.9200566166301414 1105 40-41 0.782385489074052 1105 42-43 0.8486606980352676 1105 44-45 0.7331730394572489 1105 46-47 0.4920280321515449 1105 48-49 0.5831878497631493 1105 50-51 0.4441105677541941 1105 52-53 0.6270741007292209 1105 54-55 0.6396918626017793 1105 56-57 0.3562907748152604 1105 58-59 0.49672951137116783 1105 60-61 0.5678666996264212 1105 62-63 0.5852800461118299 1105 64-65 0.2977946851296771 1105 66-67 0.33448403312005937 1105 68-69 0.29721515444728297 1105 70-71 0.5379462025861734 1105 72-73 0.48833438005624785 1105 74-75 0.45255360112305354 1105 76-77 0.2301444380976143 1105 78-79 0.21477473499779975 1105 80-81 0.1255623794080094 1105 82-83 0.21929380003952303 1105 84-85 -0.033914150965784984 1105 86-87 -0.09895832851517028 1105 88-89 -0.08431280527875273 1105 90-91 0.019886351100783628 1105 92-93 0.2280158626253268 1105 94-95 0.4304948841483274 1105 96-97 1.7953016609424708 1105 98-99 1.920291993741202 1105 100-101 1.4673738429661682 1106 1 0.877869520914949 1106 2 0.9375820282382747 1106 3 0.9422675474432047 1106 4 0.7954177944258731 1106 5 0.8277963724805488 1106 6 0.9496893293786499 1106 7 1.0189165813300036 1106 8 0.9611632825242253 1106 9 1.253309109039769 1106 10-11 1.1454172204785422 1106 12-13 1.1876369420947839 1106 14-15 1.4465634461400256 1106 16-17 1.4843725150573377 1106 18-19 1.4549075981700312 1106 20-21 1.3509833144831518 1106 22-23 1.0055851081151843 1106 24-25 1.4132512772551422 1106 26-27 1.4869952010780807 1106 28-29 1.2928697071676467 1106 30-31 1.2909694986640758 1106 32-33 1.2759145446857545 1106 34-35 1.4435951331300245 1106 36-37 1.372637051363455 1106 38-39 1.0500258874634767 1106 40-41 1.3847046296990513 1106 42-43 1.1188448126185975 1106 44-45 1.4900747321655814 1106 46-47 1.2472043342348726 1106 48-49 1.139067537263145 1106 50-51 1.2630114792125227 1106 52-53 1.4156315226042189 1106 54-55 1.3531335292684474 1106 56-57 1.3884859571069263 1106 58-59 1.1756481311628342 1106 60-61 1.4792379235847548 1106 62-63 1.4709175461118313 1106 64-65 1.5111222892963454 1106 66-67 1.3366487466617265 1106 68-69 1.3537940606972825 1106 70-71 1.427182140086174 1106 72-73 1.4395151092229135 1106 74-75 1.2949779500813854 1106 76-77 1.298635583930949 1106 78-79 1.1119537714561325 1106 80-81 1.017200139824677 1106 82-83 1.2199099458728568 1106 84-85 1.1239355886175488 1106 86-87 1.058383338151497 1106 88-89 1.0362292520129124 1106 90-91 1.2964659083924523 1106 92-93 1.5416847428336595 1106 94-95 1.7299899362316609 1106 96-97 3.108173275525804 1106 98-99 3.2406400406161993 1106 100-101 2.609439337757834 1107 1 0.4514052257897845 1107 2 0.55419072389045 1107 3 0.5023097081810164 1107 4 0.23858617387251257 1107 5 0.6822706807809453 1107 6 0.6905048761507189 1107 7 0.5671985312641254 1107 8 0.38010926407890366 1107 9 0.5317836808447822 1107 10-11 0.624719460267734 1107 12-13 0.5459065073121749 1107 14-15 0.6127272142559619 1107 16-17 0.6864984702615473 1107 18-19 0.5146041726100847 1107 20-21 0.4190515621774935 1107 22-23 0.344250720763398 1107 24-25 0.4102772324593573 1107 26-27 0.7040363077974519 1107 28-29 0.5740623290385258 1107 30-31 0.571991106042212 1107 32-33 0.7970127001534806 1107 34-35 0.7621040922868119 1107 36-37 0.5232294097297228 1107 38-39 0.4990269414819224 1107 40-41 0.5560362502524114 1107 42-43 0.39142294173586123 1107 44-45 0.48308329606545186 1107 46-47 0.12223700880668531 1107 48-49 0.29671536335010273 1107 50-51 0.2571133237448038 1107 52-53 0.390467095726752 1107 54-55 0.38172496536857636 1107 56-57 0.3585034801635807 1107 58-59 0.09430952773727341 1107 60-61 0.1454792938087337 1107 62-63 0.09043691370076345 1107 64-65 0.10335838942809517 1107 66-67 0.1317903803903171 1107 68-69 0.06469485121111873 1107 70-71 0.21227225866324773 1107 72-73 0.1276199840582244 1107 74-75 0.09832485390220214 1107 76-77 0.05586470119049736 1107 78-79 -0.01665031286534102 1107 80-81 4.619403071615125E-6 1107 82-83 0.036676612539523035 1107 84-85 -0.030793396889698954 1107 86-87 0.014369240654790616 1107 88-89 0.13111475925929028 1107 90-91 0.19748659350443987 1107 92-93 0.425735994480565 1107 94-95 0.7668396068508976 1107 96-97 2.1647375706509706 1107 98-99 2.340992609786163 1107 100-101 2.0800461888777306 1108 1 0.4902668218596169 1108 2 0.5134848622463721 1108 3 0.39325000358355666 1108 4 0.5731643531303234 1108 5 0.7192660081080788 1108 6 0.6287999906472095 1108 7 0.470642627213941 1108 8 0.6222901381247681 1108 9 0.7255935894716217 1108 10-11 0.4166514984812366 1108 12-13 0.415856780151465 1108 14-15 0.5228882774490629 1108 16-17 0.7195262262584166 1108 18-19 0.4851239274547865 1108 20-21 0.33412933337654493 1108 22-23 0.4922960392892719 1108 24-25 0.4238211827882097 1108 26-27 0.41103811605784557 1108 28-29 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1.1173217738298469 2311 84-85 1.3090010456067986 2311 86-87 1.4567372360009578 2311 88-89 1.7351097627656031 2311 90-91 1.9827060965644954 2311 92-93 2.005903022403551 2311 94-95 2.4849461190273594 2311 96-97 4.002947200256987 2311 98-99 4.261435201906522 2311 100-101 3.937281542058912 2312 1 0.05185926450469225 2312 2 -0.40867438201813755 2312 3 -0.7358862987106427 2312 4 -0.1891770773690027 2312 5 -1.9138446531604814 2312 6 -0.40661836292903786 2312 7 -0.09503213661871257 2312 8 0.1616761030370455 2312 9 0.09821167314233747 2312 10-11 0.10742747688879462 2312 12-13 0.12234463440247367 2312 14-15 0.3564813948579726 2312 16-17 -0.4270223567375382 2312 18-19 -0.2573693249068967 2312 20-21 0.1670038273036667 2312 22-23 0.15176972349979678 2312 24-25 0.06169230289616934 2312 26-27 0.21810289338577604 2312 28-29 0.26566970716764615 2312 30-31 -0.031892039797462246 2312 32-33 -0.6847008399296257 2312 34-35 -0.13084589251100454 2312 36-37 0.262165256491663 2312 38-39 0.2862515284891174 2312 40-41 0.44873539892981995 2312 42-43 0.38968583825963066 2312 44-45 0.31663883472968024 2312 46-47 0.013378693209240566 2312 48-49 0.43473933213493865 2312 50-51 0.41472429972534997 2312 52-53 0.32920075337345267 2312 54-55 0.2899642984992141 2312 56-57 0.3086603160812835 2312 58-59 0.5031865927012937 2312 60-61 0.5451661287129603 2312 62-63 0.2967944691887503 2312 64-65 0.42756331493736965 2312 66-67 0.4392128492258287 2312 68-69 0.3613632914665139 2312 70-71 0.4670898323938637 2312 72-73 0.6293510066588119 2312 74-75 0.6898189757224138 2312 76-77 0.6199791736745404 2312 78-79 0.7537999253022853 2312 80-81 0.8242668064913445 2312 82-83 0.961356099719012 2312 84-85 1.2089919988739588 2312 86-87 1.0757371843053427 2312 88-89 0.9764241238077851 2312 90-91 1.1405992417257842 2312 92-93 1.0957975633464798 2312 94-95 1.612666859308586 2312 96-97 2.992296352448882 2312 98-99 3.380886194462356 2312 100-101 3.08296754288604 2313 1 -0.03946217391352036 2313 2 -0.49582708594503444 2313 3 -0.891002293826638 2313 4 -0.15114564643434392 2313 5 -0.09886414979288105 2313 6 0.12540259102739526 2313 7 0.08467797405914013 2313 8 0.27256113198659193 2313 9 0.44840506398088564 2313 10-11 -0.5695767375347813 2313 12-13 -0.08458671381919203 2313 14-15 0.16798362023116198 2313 16-17 -0.28667141735434143 2313 18-19 -0.09606915554220308 2313 20-21 -2.1160573409187933 2313 22-23 -0.31287756469588146 2313 24-25 0.1389442624061914 2313 26-27 0.031668216951096895 2313 28-29 -0.16549260722047165 2313 30-31 0.0413928473583951 2313 32-33 0.27463036649835004 2313 34-35 0.040570862776718286 2313 36-37 -0.6239511718828297 2313 38-39 0.319429676505969 2313 40-41 0.3187519415270046 2313 42-43 0.2887811157420046 2313 44-45 0.44990678541699225 2313 46-47 0.287184672176501 2313 48-49 0.4924635434075384 2313 50-51 0.7067870040461166 2313 52-53 0.7679194898341244 2313 54-55 0.7144406465239541 2313 56-57 0.810110944306107 2313 58-59 0.750808397419874 2313 60-61 0.3594234842606383 2313 62-63 0.36799814006984377 2313 64-65 0.4242622790556858 2313 66-67 0.5603558639172306 2313 68-69 0.2917000514806922 2313 70-71 0.5701277622059884 2313 72-73 0.6389965224333594 2313 74-75 0.6911159941162062 2313 76-77 0.8375218102494308 2313 78-79 0.7343726142620746 2313 80-81 0.8870778663991743 2313 82-83 1.0715908470505298 2313 84-85 0.9434616510855456 2313 86-87 0.8467261952943552 2313 88-89 1.3004459442812184 2313 90-91 1.2613519298978275 2313 92-93 1.5102873029975115 2313 94-95 1.8584020202050375 2313 96-97 3.0515023077838705 2313 98-99 2.994362109228593 2313 100-101 2.716011380768588 2314 1 -0.35500979603922644 2314 2 -0.2959820073079875 2314 3 -1.262835780662737 2314 4 -0.22022590656732888 2314 5 -0.5133055616543203 2314 6 -0.5173286179989418 2314 7 -0.35832405292683944 2314 8 -1.7690893747331344 2314 9 -1.4646607114864523 2314 10-11 -0.7038634207481564 2314 12-13 -0.45405087808294553 2314 14-15 -0.7466658969511073 2314 16-17 -0.7577244356309407 2314 18-19 -1.7337086764422693 2314 20-21 -1.207315204422578 2314 22-23 -1.990175268257012 2314 24-25 -1.6958967973223125 2314 26-27 -1.7455743755032032 2314 28-29 -2.8935531539579884 2314 30-31 -1.4881804577743267 2314 32-33 -1.3032288600885948 2314 34-35 -1.633676726078896 2314 36-37 -3.0532800770561295 2314 38-39 -3.4183113141395935 2314 40-41 -4.242633373437386 2314 42-43 -2.9388598746091397 2314 44-45 -3.0193213298661306 2314 46-47 -3.0465609907346263 2314 48-49 -1.3954069007922598 2314 50-51 -0.9251856282974913 2314 52-53 -2.2801832186398983 2314 54-55 -1.7117930433051711 2314 56-57 -1.6131594863501206 2314 58-59 -3.4583745208671353 2314 60-61 -2.0311626340036746 2314 62-63 -1.963485799418752 2314 64-65 -1.351398846790083 2314 66-67 -2.587519226852823 2314 68-69 -2.2512982550371667 2314 70-71 -1.7212920888735788 2314 72-73 -0.9853892992106132 2314 74-75 -1.4128511490648066 2314 76-77 -0.4718222658686706 2314 78-79 -0.2370805550815902 2314 80-81 -0.22361435747450287 2314 82-83 -0.14984325154829747 2314 84-85 -0.08417955339325545 2314 86-87 0.20399351414034683 2314 88-89 0.5715381211538819 2314 90-91 0.6044936484168453 2314 92-93 0.9869906149368148 2314 94-95 1.3058070472266046 2314 96-97 2.93361338704349 2314 98-99 3.0264022988493444 2314 100-101 2.6484178357540014 2315 1 -2.150174073034389 2315 2 -1.6014360144079802 2315 3 -1.4114966224128196 2315 4 -1.1755670060115264 2315 5 -1.4409975039546623 2315 6 -2.902967495844603 2315 7 -1.8402181006455791 2315 8 -1.0073732583906647 2315 9 -0.9415643008381238 2315 10-11 -3.081036725211277 2315 12-13 -1.6826366500320589 2315 14-15 -0.8769841390613635 2315 16-17 -1.6203432130272333 2315 18-19 -1.0877551319192733 2315 20-21 -0.7934419816713927 2315 22-23 -1.1304984348044513 2315 24-25 -2.9545537892657237 2315 26-27 -3.9751834757945232 2315 28-29 -5.429471535770208 2315 30-31 -8.16715202493716 2315 32-33 -6.567320738711363 2315 34-35 -5.830774394845193 2315 36-37 -3.513425860974799 2315 38-39 -5.978998643245475 2315 40-41 -3.6852575354185007 2315 42-43 -3.697866978888598 2315 44-45 -2.757618360599132 2315 46-47 -1.8294156038004772 2315 48-49 -2.7632707896914717 2315 50-51 -2.493370077193525 2315 52-53 -1.680994393196766 2315 54-55 -3.1733634271740527 2315 56-57 -3.0862371338353007 2315 58-59 -1.8942530899051455 2315 60-61 -2.3005780778732365 2315 62-63 -2.9058395889346436 2315 64-65 -2.0861823216021413 2315 66-67 -2.4329703530284483 2315 68-69 -1.828935737006379 2315 70-71 -2.2056279200559814 2315 72-73 -1.0936963360112735 2315 74-75 -1.6974604679977112 2315 76-77 -0.9998774104193338 2315 78-79 -1.0322532633534145 2315 80-81 -0.6838560293023512 2315 82-83 -0.5174854979033441 2315 84-85 -0.5275173729279246 2315 86-87 -1.110106838629985 2315 88-89 -1.446679295462939 2315 90-91 -0.9637255259068027 2315 92-93 -0.010875271746257908 2315 94-95 0.5291215564248439 2315 96-97 2.1123734577747584 2315 98-99 1.840183835039383 2315 100-101 1.3379359297024322 2316 1 -3.14615499328535 2316 2 -1.9156377027034281 2316 3 -2.0383348441860925 2316 4 -2.6523149410001388 2316 5 -2.1515220131696786 2316 6 -5.235304691548105 2316 7 -2.9036379777133448 2316 8 -4.30837333929939 2316 9 -5.977273551647823 2316 10-11 -5.248997727204575 2316 12-13 -3.1673173179949003 2316 14-15 -4.637613982858486 2316 16-17 -3.8777872756751037 2316 18-19 -2.1352079474204047 2316 20-21 -2.9626926197470382 2316 22-23 -3.926103681122491 2316 24-25 -2.8661721906073367 2316 26-27 -3.7636330500429906 2316 28-29 -3.361299650081982 2316 30-31 -3.2102952248037866 2316 32-33 -3.2457543641333757 2316 34-35 -4.87345972486699 2316 36-37 -5.077114667620101 2316 38-39 -5.0299460108922815 2316 40-41 -4.500372051915299 2316 42-43 -3.86347013506451 2316 44-45 -3.378933489060131 2316 46-47 -3.2036150977531648 2316 48-49 -3.7542692340372987 2316 50-51 -4.55437970165444 2316 52-53 -2.6228240827769476 2316 54-55 -3.1130407607166077 2316 56-57 -2.4989425929678113 2316 58-59 -3.176184454786341 2316 60-61 -3.2121407012283996 2316 62-63 -2.3453183283276324 2316 64-65 -2.7486905657111294 2316 66-67 -3.4927421352515786 2316 68-69 -2.8627243249529393 2316 70-71 -2.4372806401828875 2316 72-73 -2.249583396008777 2316 74-75 -3.051117266660018 2316 76-77 -3.1614833996266007 2316 78-79 -3.147023059635046 2316 80-81 -3.4570710111469225 2316 82-83 -2.426327871765409 2316 84-85 -2.272434067585742 2316 86-87 -1.9321281715644965 2316 88-89 -1.8829230648779678 2316 90-91 -1.554613762758521 2316 92-93 -1.3204476936386875 2316 94-95 -1.2122299441868734 2316 96-97 0.963530973582607 2316 98-99 1.392813583217098 2316 100-101 0.6893677383557417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15847.0 3 3381.0 4 2874.0 5 2992.0 6 3740.0 7 4537.0 8 5832.0 9 7647.0 10 9653.0 11 13122.0 12 17867.0 13 21694.0 14 24610.0 15 29056.0 16 33902.0 17 37270.0 18 28726.0 19 23735.0 20 25116.0 21 27572.0 22 30619.0 23 34446.0 24 39619.0 25 45043.0 26 51925.0 27 59260.0 28 67764.0 29 77063.0 30 87531.0 31 99743.0 32 115655.0 33 131923.0 34 153099.0 35 175233.0 36 189966.0 37 168157.0 38 48441.0 39 489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99345166617612 23.533590924132696 14.237633524374324 20.235323885316852 2 14.55005282364743 23.567344792309754 41.717560504116896 20.165041879925916 3 17.13899429400761 31.055362869538033 32.92159985277557 18.884042983678793 4 10.32478825545924 19.386848046951393 43.33680532679093 26.951558370798445 5 9.635352125195421 41.71181628523474 36.910636695894965 11.74219489367487 6 23.806127635010636 42.746204476197846 20.32552930276139 13.122138586030129 7 23.304092099428562 37.22134024413711 24.946453820603566 14.52811383583076 8 24.285960051098286 35.70861219425087 22.65501913048207 17.35040862416878 9 27.064803581140445 16.811122034891017 24.384282307660126 31.739792076308415 10-11 21.544183069525392 29.23283435594371 30.87254937591004 18.350433198620856 12-13 23.167139553830175 28.708259820557362 30.511183528675996 17.613417096936466 14-15 21.406779793944878 28.882513025496774 29.83381217665005 19.876895003908302 16-17 17.557117598816433 32.168728907121924 31.07747434214534 19.196679151916303 18-19 17.360548565021556 30.553517460878606 34.88277047927794 17.2031634948219 20-21 18.296079120159387 29.875675719505423 34.63246377918505 17.195781381150134 22-23 18.36735708272488 29.80644212753556 33.86300053943388 17.96320025030569 24-25 17.763682420631195 30.83665663896642 34.39437513786234 17.005285802540044 26-27 17.667299732521393 30.13198088556301 35.19178022688843 17.00893915502717 28-29 16.96462635802369 29.861553413199786 35.591799758527046 17.582020470249475 30-31 17.476758328483687 30.182144367361808 35.06532893166813 17.275768372486382 32-33 17.3976414608017 30.415792886491705 34.84534156322174 17.341224089484857 34-35 17.886151639114832 30.315763534684866 34.446655194946864 17.351429631253442 36-37 18.15732638831672 29.669622588357893 34.2557608562312 17.91729016709419 38-39 17.09851743299359 30.657933907343875 34.246816512124695 17.996732147537838 40-41 17.161159202698304 29.98120657207613 35.22612927315077 17.6315049520748 42-43 17.041780962198576 30.028403970958315 34.584836166450046 18.344978900393063 44-45 17.14548645417003 30.078943988456743 34.252153570764335 18.5234159866089 46-47 17.635537424477583 30.400147146396012 33.32564983553303 18.638665593593377 48-49 17.065011111355354 30.54861204883344 33.26709960652299 19.11927723328822 50-51 16.79639274350565 30.26717578230203 33.32493182608723 19.611499648105095 52-53 16.836929566056707 31.0570471248158 33.10559476129728 19.000428547830207 54-55 16.92598779058612 30.481730248965555 32.82395769609025 19.768324264358075 56-57 17.039179973517143 30.70606122459661 32.58315145995907 19.671607341927178 58-59 17.060207293746384 29.70274464037597 32.9742021074777 20.26284595839995 60-61 17.27281193852426 30.20630914298331 32.314083185001714 20.20679573349072 62-63 17.19392470253533 29.492156590391932 32.700749249717035 20.613169457355703 64-65 17.196765681954194 29.396889764932855 32.58509518608262 20.821249367030337 66-67 17.109229024516296 29.47385270135397 31.804647990174235 21.6122702839555 68-69 17.2443476077381 29.359456575189107 31.818783414766845 21.577412402305946 70-71 17.352920250649483 29.502844342233438 31.997594576864202 21.146640830252878 72-73 17.854400916895845 28.651948534510506 31.796686719402135 21.696963829191514 74-75 17.54735176851904 29.43303494904001 31.31192695364074 21.707686328800214 76-77 17.76481760391915 29.157145467176136 31.638677612054376 21.439359316850336 78-79 18.107303274625384 28.851973155599016 31.221508183815367 21.819215385960238 80-81 18.286317023760294 28.769924568006576 30.999539346605037 21.944219061628097 82-83 18.541708269941502 28.8666574365194 30.96011241586542 21.63152187767368 84-85 18.537527427739274 28.411827586973903 30.681751868749846 22.36889311653698 86-87 18.63437718646858 28.05741898716888 30.97763829732676 22.330565529035777 88-89 19.42732233116355 27.651004217722285 30.925070341162158 21.99660310995201 90-91 19.42967402206226 27.61393737085057 31.102152767438263 21.854235839648904 92-93 20.186713142145358 27.34880422466288 30.644794929567144 21.819687703624616 94-95 20.185541439062312 26.739578570903298 30.612165319207012 22.462714670827378 96-97 20.78775517905632 26.174596993914527 30.201798939802245 22.83584888722691 98-99 20.72860870669598 26.179766601157173 29.888878268873476 23.20274642327337 100-101 21.02539041272221 26.164468193040364 30.13471068904189 22.67543070519554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3371.0 1 2059.0 2 832.5 3 944.0 4 817.0 5 798.5 6 1238.0 7 2115.0 8 3168.0 9 3458.5 10 2972.0 11 2719.0 12 2886.5 13 3082.5 14 3277.0 15 3550.5 16 3973.5 17 4537.0 18 5115.0 19 5791.0 20 6711.5 21 7744.5 22 8886.0 23 10291.5 24 11909.0 25 14659.5 26 19754.0 27 25201.0 28 27835.0 29 30838.0 30 36653.0 31 42771.5 32 51391.0 33 64421.5 34 80095.5 35 91805.0 36 101689.0 37 110937.0 38 110682.0 39 104850.0 40 97887.5 41 91597.0 42 86867.5 43 82996.0 44 79882.5 45 75264.0 46 67835.0 47 59821.5 48 56212.0 49 50376.0 50 39113.0 51 29153.5 52 21671.5 53 16066.0 54 11967.0 55 8856.0 56 6466.0 57 4801.0 58 3508.5 59 2560.5 60 1926.0 61 1420.0 62 994.5 63 756.0 64 599.5 65 467.5 66 421.5 67 379.5 68 280.0 69 198.5 70 160.5 71 132.0 72 91.0 73 67.5 74 54.5 75 34.0 76 23.5 77 22.0 78 17.0 79 11.0 80 10.0 81 9.5 82 7.0 83 4.0 84 1.5 85 1.5 86 2.0 87 1.0 88 1.5 89 3.0 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11988623339489511 2 0.11382926341501366 3 0.12651757121769638 4 0.14113784358292747 5 0.13675176187335814 6 0.13748277549161972 7 0.1452106337418133 8 0.14390525228063195 9 0.1518941868230618 10-11 0.14643769231532378 12-13 0.1453150642587078 14-15 0.13335777007428665 16-17 0.13709116105326533 18-19 0.15455716500387176 20-21 0.1866956565781566 22-23 0.20290849432602892 24-25 0.22324633749123435 26-27 0.22671865217797674 28-29 0.2284939709651834 30-31 0.22750188105468555 32-33 0.22922498458344495 34-35 0.19747810744751454 36-37 0.18601685821834227 38-39 0.1985224126164596 40-41 0.18674787183660385 42-43 0.189045343208283 44-45 0.19478902163748094 46-47 0.21549237161181717 48-49 0.23449872568661762 50-51 0.24945839723175584 52-53 0.27206760413941683 54-55 0.264496391664565 56-57 0.23387214258525055 58-59 0.2263009301103987 60-61 0.20316957061826524 62-63 0.19337920965940508 64-65 0.18405878602657025 66-67 0.17967270431700091 68-69 0.18635625739824943 70-71 0.18980246445576818 72-73 0.2273452352793438 74-75 0.23922420657609406 76-77 0.2578389462125401 78-79 0.2470826029724058 80-81 0.25162533045731694 82-83 0.24763086318610197 84-85 0.2321751466857148 86-87 0.2218104178839349 88-89 0.22366405955881238 90-91 0.22606596144738605 92-93 0.23308891370854173 94-95 0.23906756080075234 96-97 8.679742411687027 98-99 0.3005771352516175 100-101 0.31242999891914414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1915149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.23297705158924 #Duplication Level Percentage of deduplicated Percentage of total 1 77.56664740494085 25.00203965772172 2 10.414386105698856 6.71373336702762 3 4.032991270578228 3.8998594522142325 4 2.0578939438399524 2.6532819306559063 5 1.2453352388488463 2.0070431087675136 6 0.8184512745230185 1.5828672689726653 7 0.5549136987931832 1.2520564363169224 8 0.41648939827916426 1.073975457357001 9 0.29811939998999953 0.8648348200660088 >10 1.9464002095306778 12.253635045590123 >50 0.2915105191852689 6.645733927654911 >100 0.307039433807362 20.10736424680601 >500 0.036311023480264 8.108399262939534 >1k 0.013004413060373619 6.115540814234681 >5k 1.6888848130355348E-4 0.39102661268157124 >10k+ 3.3777696260710695E-4 1.3286085909935164 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14315 0.7474614246724407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10091 0.5269041729912398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7183 0.3750622014266253 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2743 0.14322645392081765 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2493 0.13017263930900416 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2441 0.12745744586974694 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2299 0.1200428791702369 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2282 0.11915521977663357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2256 0.11779762305700496 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2225 0.11617895004514009 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2213 0.11555236694377304 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2181 0.11388147867346092 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 2155 0.1125238819538323 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2077 0.1084510917949465 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2047 0.10688463404152888 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 2017 0.10531817628811127 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2003 0.1045871626698497 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1959 0.10228969129817053 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1929 0.10072323354475292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.2215258447253975E-5 0.0 6 0.0 0.0 0.0 5.2215258447253975E-5 0.0 7 0.0 0.0 0.0 5.2215258447253975E-5 0.0 8 0.0 0.0 0.0 5.2215258447253975E-5 0.0 9 0.0 0.0 0.0 5.2215258447253975E-5 0.0 10-11 0.0 0.0 0.0 7.832288767088096E-5 0.0 12-13 0.0 0.0 0.0 1.0443051689450795E-4 0.0 14-15 0.0 0.0 0.0 1.827534045653889E-4 0.0 16-17 0.0 0.0 0.0 3.9161443835440484E-4 0.0 18-19 0.0 0.0 0.0 4.699373260252858E-4 0.0 20-21 0.0 0.0 0.0 8.093365059324366E-4 0.0 22-23 0.0 0.0 0.0 0.002062502708666532 0.0 24-25 0.0 0.0 0.0 0.006422476789012239 0.0 26-27 0.0 0.0 0.0 0.01420255029765308 0.0 28-29 0.0 0.0 0.0 0.03529751471034369 0.0 30-31 0.0 0.0 0.0 0.11727547047253242 0.0 32-33 0.0 0.0 0.0 0.3193224130341817 0.0 34-35 0.0 0.0 0.0 0.6584866242783198 0.0 36-37 0.0 0.0 0.0 1.191604413024783 0.0 38-39 0.0 0.0 0.0 1.9397707436862615 0.0 40-41 0.0 0.0 0.0 2.779261561371987 0.0 42-43 0.0 0.0 0.0 3.566119398542881 0.0 44-45 0.0 0.0 0.0 4.459940192642975 0.0 46-47 0.0 0.0 0.0 5.478111624735203 0.0 48-49 0.0 0.0 0.0 6.542154161373345 0.0 50-51 0.0 0.0 0.0 7.663607374674243 0.0 52-53 0.0 0.0 0.0 8.669377682885248 0.0 54-55 0.0 0.0 0.0 9.679194673625917 0.0 56-57 0.0 0.0 0.0 10.839939868908372 0.0 58-59 0.0 0.0 0.0 12.045929585635374 0.0 60-61 0.0 2.6107629223626988E-5 2.6107629223626988E-5 13.31335055392557 0.0 62-63 0.0 5.2215258447253975E-5 5.2215258447253975E-5 14.465428016305781 0.0 64-65 0.0 5.2215258447253975E-5 5.2215258447253975E-5 15.594269688676965 0.0 66-67 0.0 5.2215258447253975E-5 5.2215258447253975E-5 16.80720403477745 0.0 68-69 0.0 5.2215258447253975E-5 5.2215258447253975E-5 18.06055299091611 0.0 70-71 0.0 5.2215258447253975E-5 5.2215258447253975E-5 19.399508863279046 0.0 72-73 0.0 5.2215258447253975E-5 5.2215258447253975E-5 20.66116004551082 0.0 74-75 0.0 5.2215258447253975E-5 5.2215258447253975E-5 21.877618921556497 0.0 76-77 0.0 5.2215258447253975E-5 5.2215258447253975E-5 23.107914841090693 0.0 78-79 0.0 5.2215258447253975E-5 5.2215258447253975E-5 24.408283637461107 0.0 80-81 0.0 5.2215258447253975E-5 5.2215258447253975E-5 25.755045690961904 0.0 82-83 0.0 7.832288767088096E-5 5.2215258447253975E-5 27.0157308909124 0.0 84-85 0.0 1.0443051689450795E-4 5.2215258447253975E-5 28.189556008435897 0.0 86-87 0.0 1.0443051689450795E-4 5.2215258447253975E-5 29.250752813488663 0.0 88-89 0.0 1.0443051689450795E-4 5.2215258447253975E-5 30.2887138285324 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 790 0.0 42.28361 9 CCTTTAG 855 0.0 38.51376 1 TTAGGCA 945 0.0 36.342976 4 TAGGCAT 945 0.0 36.342976 5 AGCGCCA 55 0.002703902 34.216698 8 GGCATTC 990 0.0 33.74058 7 CATGGGT 1545 0.0 33.191544 4 GTATCAA 18245 0.0 31.894058 1 AAGCGCC 60 0.0041414746 31.364485 7 CTTTAGG 1085 0.0 31.218317 2 GTACATG 13540 0.0 31.094841 1 GGTATCA 12025 0.0 30.122404 1 GACATAT 1120 0.0 29.821144 1 ACATGGG 13655 0.0 29.423584 3 TACATGG 14300 0.0 29.312197 2 CATGGGG 4285 0.0 28.76603 4 GGCGCGG 40 0.006817038 28.325092 92-93 CTAGTCG 155 5.0384187E-8 27.317455 4 AGGCATT 1275 0.0 27.305555 6 CATATAG 1215 0.0 26.717894 3 >>END_MODULE