##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939834_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1915149 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.327375050191918 31.0 31.0 34.0 30.0 34.0 2 31.547293187109723 31.0 31.0 34.0 30.0 34.0 3 31.56877767735043 31.0 31.0 34.0 30.0 34.0 4 35.40559246304073 37.0 35.0 37.0 33.0 37.0 5 35.17158038356285 37.0 35.0 37.0 32.0 37.0 6 35.20390110638911 37.0 35.0 37.0 32.0 37.0 7 35.115003584577494 37.0 35.0 37.0 32.0 37.0 8 35.09094697070567 37.0 35.0 37.0 32.0 37.0 9 36.60290243735605 39.0 35.0 39.0 32.0 39.0 10-11 36.49385217547042 38.0 35.0 39.0 32.0 39.0 12-13 36.44085708213825 38.0 35.0 39.0 32.0 39.0 14-15 37.363944267521745 39.0 36.0 41.0 32.0 41.0 16-17 37.30227726406666 39.0 36.0 41.0 32.0 41.0 18-19 37.41892510713266 39.0 36.0 41.0 32.0 41.0 20-21 37.40213241893973 39.0 36.0 41.0 32.0 41.0 22-23 37.264297451529885 39.0 36.0 40.5 31.0 41.0 24-25 37.107416707525104 39.0 36.0 40.0 31.0 41.0 26-27 36.792161863123965 39.0 35.5 40.0 30.5 41.0 28-29 36.573618553961076 38.5 35.0 40.0 30.0 41.0 30-31 36.24353823122901 38.0 35.0 40.0 30.0 41.0 32-33 36.09971469582784 38.0 35.0 40.0 30.0 41.0 34-35 35.89096305300528 38.0 35.0 40.0 30.0 41.0 36-37 35.76044605406681 38.0 35.0 40.0 29.0 41.0 38-39 35.59665488168284 38.0 35.0 40.0 29.0 41.0 40-41 35.482583339468626 38.0 35.0 40.0 27.5 41.0 42-43 35.17661210694311 38.0 34.0 40.0 27.0 41.0 44-45 34.78180862167905 38.0 34.0 40.0 24.5 41.0 46-47 34.315399219590745 38.0 33.0 40.0 23.5 41.0 48-49 33.88810139576607 38.0 33.0 40.0 21.0 41.0 50-51 33.67070734444161 38.0 33.0 40.0 19.0 41.0 52-53 33.4509777046068 38.0 33.0 40.0 17.0 41.0 54-55 33.21336042260941 38.0 33.0 40.0 14.5 41.0 56-57 32.87311143937104 37.5 32.0 40.0 10.5 41.0 58-59 32.54007155579018 37.0 31.0 40.0 8.0 41.0 60-61 32.23901377908455 37.0 31.0 40.0 7.5 41.0 62-63 31.95734196138264 37.0 31.0 39.5 2.0 41.0 64-65 31.538611095011404 36.0 30.5 39.0 2.0 40.5 66-67 30.641283524153998 35.5 29.0 39.0 2.0 40.0 68-69 28.752928884384453 34.0 24.5 38.0 2.0 40.0 70-71 28.107820070396613 34.0 23.5 37.0 2.0 39.5 72-73 28.61247819360269 34.0 26.0 37.0 2.0 39.0 74-75 28.63625806660474 34.0 26.0 36.5 2.0 39.0 76-77 28.041453432604982 32.5 26.0 35.5 2.0 38.5 78-79 28.48638147736808 34.0 27.0 36.0 2.0 38.5 80-81 28.2116122557566 34.0 26.5 35.5 2.0 37.5 82-83 27.707758769683196 33.5 26.0 35.0 2.0 37.0 84-85 27.305651152991228 33.0 26.0 35.0 2.0 36.5 86-87 26.91308822446713 33.0 25.5 35.0 2.0 36.0 88-89 26.433624746690725 32.5 24.5 35.0 2.0 36.0 90-91 25.9209043265041 32.0 23.5 35.0 2.0 35.0 92-93 25.429271299517687 32.0 20.5 34.5 2.0 35.0 94-95 24.631742752130513 31.0 17.5 34.0 2.0 35.0 96-97 24.11219335936786 31.0 6.5 34.0 2.0 35.0 98-99 23.460420050868105 31.0 2.0 34.0 2.0 35.0 100-101 21.978126767160152 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3308413493791704 1101 2 0.3911163219100544 1101 3 0.3477962356634343 1101 4 0.19252688465664392 1101 5 0.2914148639201741 1101 6 0.29768971395430555 1101 7 0.23736796743940403 1101 8 0.3443039403578467 1101 9 0.3803251566046697 1101 10-11 0.5018475350695937 1101 12-13 0.46833434906789506 1101 14-15 0.41223533215947583 1101 16-17 0.4590218560967898 1101 18-19 0.39183425874103506 1101 20-21 0.48089910914745104 1101 22-23 0.5632841977592946 1101 24-25 0.44540952749968454 1101 26-27 0.5851147717322789 1101 28-29 0.5279117720428417 1101 30-31 0.5729591155943794 1101 32-33 0.5160874779459022 1101 34-35 0.3837219720947047 1101 36-37 0.667982506198534 1101 38-39 0.620366073443158 1101 40-41 0.8546645028562381 1101 42-43 0.40469262946364637 1101 44-45 0.06738021116537851 1101 46-47 0.04327737239006524 1101 48-49 0.021980205137730024 1101 50-51 0.14995361721130251 1101 52-53 -0.01415365607798691 1101 54-55 -0.28985697254034193 1101 56-57 -0.2096956695890171 1101 58-59 -0.15920577476323672 1101 60-61 -0.2665085750112368 1101 62-63 0.020466679697577206 1101 64-65 -0.15277765995020332 1101 66-67 -0.0680877044219308 1101 68-69 -8.315260109269655 1101 70-71 -5.709152637920777 1101 72-73 -1.4784020742386446 1101 74-75 -0.45252764637303144 1101 76-77 -0.45370584136496106 1101 78-79 -0.5207572226200874 1101 80-81 -0.5616098766179753 1101 82-83 -0.526752806989645 1101 84-85 -0.15022301987912812 1101 86-87 -0.09231951084648671 1101 88-89 -0.18542991343669613 1101 90-91 -0.5494419761966292 1101 92-93 -0.571019613360928 1101 94-95 -0.4479423773023079 1101 96-97 -0.7063235643986125 1101 98-99 -0.5960844048677991 1101 100-101 -0.4971913859000239 1102 1 0.12713083762784905 1102 2 0.06786663210172605 1102 3 0.16375167595578333 1102 4 0.11528016789311124 1102 5 0.35788079678290785 1102 6 0.27974355243885185 1102 7 0.2575863703469139 1102 8 0.039536379313524606 1102 9 0.3201010193970575 1102 10-11 0.3181598706080706 1102 12-13 0.24370662902162366 1102 14-15 0.32386891837477805 1102 16-17 0.30368151439643754 1102 18-19 0.22676258997977783 1102 20-21 0.21079684080218186 1102 22-23 0.3221094254829353 1102 24-25 0.25667263197383505 1102 26-27 0.34640704721045523 1102 28-29 0.26867844996498746 1102 30-31 0.1154396399531663 1102 32-33 -0.06728739742797529 1102 34-35 0.09934714067713202 1102 36-37 0.06781856915195306 1102 38-39 0.1129846605675553 1102 40-41 0.19090727753820147 1102 42-43 0.291795349360072 1102 44-45 0.3554423773721638 1102 46-47 0.11723311038650053 1102 48-49 0.16461417696972802 1102 50-51 0.10201876445494662 1102 52-53 0.06971622725797744 1102 54-55 -0.05150764177511746 1102 56-57 -0.1420891204988095 1102 58-59 -0.24982944483518565 1102 60-61 -0.14174808693638852 1102 62-63 -0.14788472900217897 1102 64-65 -0.0298770731334983 1102 66-67 0.32347857869265084 1102 68-69 -4.208316411305429 1102 70-71 0.27061547257738994 1102 72-73 0.5289473704133698 1102 74-75 0.247283990859561 1102 76-77 0.07467994316521853 1102 78-79 0.01733716156203613 1102 80-81 -0.04031554318540742 1102 82-83 0.021264713594892015 1102 84-85 -0.08020761519138375 1102 86-87 -0.16218980976935882 1102 88-89 -0.1651560656414084 1102 90-91 -0.3438278566314814 1102 92-93 -0.4157457056252163 1102 94-95 0.02969734880993613 1102 96-97 -0.6024198446959979 1102 98-99 0.02491582090778266 1102 100-101 -0.08517542976042591 1103 1 0.20569455505123457 1103 2 0.2387514640523989 1103 3 0.21603764702237882 1103 4 0.1803741909004799 1103 5 0.11526463344443982 1103 6 0.25210075387544606 1103 7 0.24014854090768267 1103 8 0.3731850144824378 1103 9 0.5008910789866476 1103 10-11 0.3988777956894012 1103 12-13 0.28102997195487234 1103 14-15 0.44864835040889517 1103 16-17 0.3303269354521561 1103 18-19 0.29284883101026793 1103 20-21 0.28024706578028 1103 22-23 0.34082054201013534 1103 24-25 0.19380750630599408 1103 26-27 0.22843788913514373 1103 28-29 0.167205638237526 1103 30-31 0.30615900477639 1103 32-33 0.46600122856196435 1103 34-35 0.34934320964126186 1103 36-37 0.26058219547358163 1103 38-39 0.5160716047620824 1103 40-41 0.4645511070157653 1103 42-43 0.15988858075509427 1103 44-45 0.22606129931507013 1103 46-47 0.20713749776649593 1103 48-49 0.2134826037249482 1103 50-51 0.2534188028808231 1103 52-53 0.20457569518244156 1103 54-55 0.1604017262273274 1103 56-57 0.3015373681967617 1103 58-59 0.2468264720863047 1103 60-61 0.1978775654313054 1103 62-63 0.06398434588258084 1103 64-65 -0.010699146096474266 1103 66-67 0.28454437096590723 1103 68-69 -3.4941926416582376 1103 70-71 0.5277714320032025 1103 72-73 0.7089078585890576 1103 74-75 0.28535459914313677 1103 76-77 0.29536178625103915 1103 78-79 0.32657013292886106 1103 80-81 0.29896884613062724 1103 82-83 0.43717838215058435 1103 84-85 0.23974148280786522 1103 86-87 0.15702627288460036 1103 88-89 -0.039220540526262226 1103 90-91 0.11722847658670688 1103 92-93 0.1496468304186962 1103 94-95 0.08913473404211558 1103 96-97 -0.12233054138857469 1103 98-99 0.12022771666457288 1103 100-101 -0.11184726246339949 1104 1 0.2290126920645399 1104 2 0.3036738588589536 1104 3 0.40111380830872534 1104 4 0.251132376891114 1104 5 0.40309427743470394 1104 6 0.34554127645430555 1104 7 0.19054287193589659 1104 8 0.2604702573668618 1104 9 0.3803202640069756 1104 10-11 0.40742235658763093 1104 12-13 0.331695348410328 1104 14-15 0.4374463988240578 1104 16-17 0.5205032172566106 1104 18-19 0.3850981302340202 1104 20-21 0.4333450165873316 1104 22-23 0.31785339474701857 1104 24-25 0.4072492225147144 1104 26-27 0.5174839045291222 1104 28-29 0.4537835351002073 1104 30-31 0.5670620675921256 1104 32-33 0.47507185294590215 1104 34-35 0.36449797722997346 1104 36-37 0.5273428284054447 1104 38-39 0.5022034787224712 1104 40-41 0.5511076821836411 1104 42-43 0.595340037561094 1104 44-45 0.6667620803725427 1104 46-47 0.7510640394742367 1104 48-49 0.8370405015117299 1104 50-51 0.6250424667854517 1104 52-53 0.6014535348664012 1104 54-55 0.6948937789626655 1104 56-57 0.6615042497809753 1104 58-59 0.5357094463038976 1104 60-61 0.7058066609293903 1104 62-63 0.893949485152234 1104 64-65 0.8065976336056586 1104 66-67 0.9422180633636401 1104 68-69 -2.8708992195214087 1104 70-71 1.2632873592610885 1104 72-73 1.4804668354557435 1104 74-75 1.187401626234685 1104 76-77 1.0389200353885464 1104 78-79 0.6748336661887784 1104 80-81 0.7461173943301702 1104 82-83 0.76874541601887 1104 84-85 0.8684999729815956 1104 86-87 0.8336907127256552 1104 88-89 0.7771094230487741 1104 90-91 0.8599828304479082 1104 92-93 0.8550512784226392 1104 94-95 1.0370456651889235 1104 96-97 0.7745067485710742 1104 98-99 1.177000338838365 1104 100-101 1.254723087578185 1105 1 0.1313564420645399 1105 2 0.1547480776089536 1105 3 0.16429740205872534 1105 4 0.199374564391114 1105 5 0.22291849618470394 1105 6 0.14681080770430555 1105 7 0.15196865318589659 1105 8 0.2311733823668618 1105 9 0.0985819827569756 1105 10-11 0.03608446596263093 1105 12-13 0.092193395285328 1105 14-15 0.11469249257405778 1105 16-17 0.26366727975661064 1105 18-19 0.07650438023402018 1105 20-21 0.1726028290873316 1105 22-23 0.16331237912201857 1105 24-25 0.2124250037647144 1105 26-27 0.2508823420291222 1105 28-29 0.1053948632252073 1105 30-31 0.16374175509212563 1105 32-33 0.06784529044590215 1105 34-35 0.26049407097997346 1105 36-37 -0.0068368590945553365 1105 38-39 0.16528941622247117 1105 40-41 0.06892994780864115 1105 42-43 -0.119015431188906 1105 44-45 -0.2394879196274573 1105 46-47 -0.2582133042757633 1105 48-49 -0.48253957661327007 1105 50-51 -0.2570376113395483 1105 52-53 -0.20396638700859882 1105 54-55 -0.13249879916233454 1105 56-57 -0.31481410959402467 1105 58-59 -0.3202475849461024 1105 60-61 -0.17480857344560974 1105 62-63 -0.29477121797276595 1105 64-65 -0.2505312726443414 1105 66-67 0.2464172821136401 1105 68-69 1.3776359367285913 1105 70-71 1.6876037655110885 1105 72-73 0.8178691792057435 1105 74-75 0.18373951685968493 1105 76-77 -0.17690027711145362 1105 78-79 -0.13961945881122162 1105 80-81 -0.046851355669829786 1105 82-83 -0.08159638085612997 1105 84-85 -0.20181252701840435 1105 86-87 -0.2100104591493448 1105 88-89 -0.16893549882622594 1105 90-91 -0.05188240392709176 1105 92-93 -0.08513426845236083 1105 94-95 0.03289527456392349 1105 96-97 -0.43374520455392584 1105 98-99 -0.06445473928663503 1105 100-101 -0.22843120929681504 1106 1 0.4115793587312062 1106 2 0.382161879692287 1106 3 0.38899089164205947 1106 4 0.2613688352244452 1106 5 0.47408099618470345 1106 6 0.3896897139543043 1106 7 0.36624625735256444 1106 8 0.3127311948668634 1106 9 0.4276298994236427 1106 10-11 0.5505020440876365 1106 12-13 0.5214803744519969 1106 14-15 0.7479327269490597 1106 16-17 0.647082644339946 1106 18-19 0.5896575052340225 1106 20-21 0.48665074575399814 1106 22-23 0.5416248791220184 1106 24-25 0.581854951681386 1106 26-27 0.6019452326541241 1106 28-29 0.5771740298918715 1106 30-31 0.6856189686337899 1106 32-33 0.8113541446125652 1106 34-35 0.6329307897299685 1106 36-37 0.8562967346554444 1106 38-39 0.7733879839308031 1106 40-41 0.8241651040586433 1106 42-43 0.7173649073527599 1106 44-45 0.7879840855808808 1106 46-47 1.0374650811409012 1106 48-49 0.9755549546367277 1106 50-51 0.9202221542854545 1106 52-53 1.0453449411164044 1106 54-55 0.9703521122959984 1106 56-57 0.8925780779059735 1106 58-59 1.0554193421372275 1106 60-61 1.220578666137719 1106 62-63 1.1413074278605677 1106 64-65 1.0949467221473235 1106 66-67 1.5155753550303075 1106 68-69 2.92751236902026 1106 70-71 3.1572636613444196 1106 72-73 2.187896522955743 1106 74-75 1.6738201158180175 1106 76-77 1.197682535388548 1106 78-79 0.9619095776471092 1106 80-81 0.975500076621838 1106 82-83 1.075295155602202 1106 84-85 1.050200493814927 1106 86-87 1.0000985252256527 1106 88-89 1.0453706209654392 1106 90-91 1.0915504085729104 1106 92-93 1.1277262784226387 1106 94-95 1.2888533474805897 1106 96-97 0.8919626079460734 1106 98-99 1.1741608857133663 1106 100-101 1.1878760823698542 1107 1 0.1274810715111805 1107 2 0.17252498904669977 1107 3 0.11472238044311212 1107 4 0.1467366876618641 1107 5 0.14068652978154006 1107 6 0.22160275743256364 1107 7 0.05918749582423999 1107 8 0.09518415929373703 1107 9 0.16665572287555364 1107 10-11 0.15899334843545887 1107 12-13 -0.039812379171188184 1107 14-15 0.20263233169213635 1107 16-17 0.11178093155997004 1107 18-19 0.08562153685457474 1107 20-21 0.1764473201940504 1107 22-23 0.10837784354889379 1107 24-25 0.11280211900680825 1107 26-27 0.02124444214029353 1107 28-29 0.16063819326473094 1107 30-31 0.05211159050467273 1107 32-33 0.11984241865737033 1107 34-35 0.13902011779320844 1107 36-37 0.02376274256058508 1107 38-39 0.18486216047889314 1107 40-41 2.4392596188249627E-5 1107 42-43 -0.07911243125066392 1107 44-45 -0.08144015684336381 1107 46-47 -0.20015415469572417 1107 48-49 -0.19479524299172368 1107 50-51 -0.26296164018095425 1107 52-53 -0.2818511063144271 1107 54-55 -0.36359189297409955 1107 56-57 -0.1964879299991651 1107 58-59 -0.010497785662508363 1107 60-61 0.014546123318218918 1107 62-63 -0.02157689361505888 1107 64-65 -0.1984752804877239 1107 66-67 0.26479248283004253 1107 68-69 1.5729223821954896 1107 70-71 1.7378639248490337 1107 72-73 0.8722305150506031 1107 74-75 0.2641235413779661 1107 76-77 0.009306646060483814 1107 78-79 0.12092683719915343 1107 80-81 0.08019151272196723 1107 82-83 0.16218237562855364 1107 84-85 0.027525263248392662 1107 86-87 -0.027471435248653364 1107 88-89 0.28273913216438373 1107 90-91 0.25351918327646317 1107 92-93 0.2267780570787643 1107 94-95 0.4729601986004859 1107 96-97 0.19348197553500412 1107 98-99 0.2759680003378726 1107 100-101 0.46827858566365776 1108 1 -0.21456018242938768 1108 2 -0.16056497590825458 1108 3 -0.15504446598277255 1108 4 -0.08838717017366093 1108 5 0.015270469868916337 1108 6 0.028458944723539048 1108 7 0.008058133196016115 1108 8 -0.028809695821394143 1108 9 -0.1517501275669133 1108 10-11 -0.1059688061148023 1108 12-13 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0.6836756194909093 2316 72-73 -0.10320661605771164 2316 74-75 -0.6351114237933437 2316 76-77 -0.9124883166293891 2316 78-79 -0.9466246525472073 2316 80-81 -1.245011433094156 2316 82-83 -0.9919157562064669 2316 84-85 -1.0754243193390316 2316 86-87 -1.0056669456263627 2316 88-89 -1.1484287811272331 2316 90-91 -1.2881711160907692 2316 92-93 -1.2211118381692891 2316 94-95 -1.1850949335358507 2316 96-97 -0.9586409794978721 2316 98-99 -1.39255346406242 2316 100-101 -1.328460987884256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 15.0 4 28.0 5 93.0 6 289.0 7 659.0 8 1390.0 9 2747.0 10 4848.0 11 7985.0 12 11368.0 13 14592.0 14 17939.0 15 22425.0 16 27651.0 17 33242.0 18 27338.0 19 19266.0 20 18055.0 21 18409.0 22 19016.0 23 20557.0 24 23626.0 25 27604.0 26 32640.0 27 39184.0 28 48312.0 29 58166.0 30 68984.0 31 81150.0 32 96823.0 33 116890.0 34 142628.0 35 179446.0 36 228383.0 37 285258.0 38 199454.0 39 18689.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.70183849201976 24.479661476382056 15.002608808738021 20.815891222860163 2 14.217623832703117 24.203626715691396 41.064825453861744 20.51392399774375 3 16.676517950570922 31.072637728259124 33.344054871999454 18.906789449170507 4 10.28645812936748 20.137649864318654 42.740852017258185 26.83503998905568 5 9.366686351819101 42.1987532040588 37.034089775782455 11.400470668339644 6 23.64630637094033 43.191417482399544 20.01369084076487 13.14858530589526 7 23.02719005153124 37.218514068618155 24.756402765528946 14.997893114321654 8 24.099795890554727 36.44593710463259 22.452769993352998 17.001497011459684 9 26.16877329126872 16.949960551372243 24.368130103715167 32.51313605364386 10-11 21.290823847126255 29.543941489669994 30.821936047795763 18.343298615407992 12-13 22.694030595008535 28.77530677769719 30.559554374098308 17.971108253195965 14-15 20.811801066131146 29.2110170018103 29.653280240858543 20.323901691200007 16-17 17.29749021221403 32.580926114094936 30.86564860464655 19.25593506904449 18-19 17.128066797935826 30.97268149893298 34.458180538433304 17.44107116469789 20-21 17.918997425265605 30.283048472990874 34.153086783325996 17.644867318417525 22-23 18.05658463127412 30.3566719874015 33.34985946263188 18.236883918692488 24-25 17.521771186113224 31.383481990407 33.915560484683674 17.1791863387961 26-27 17.4180477667429 30.76652359618618 34.7131541297242 17.10227450734672 28-29 16.61319382094268 30.357990889991964 35.195636469586006 17.833178819479343 30-31 17.32814624112042 30.578519516995765 34.74458896836965 17.34874527351416 32-33 17.210029473725985 30.95493149036171 34.36105405709532 17.473984978816983 34-35 17.646535108949987 30.80181070706659 34.120857229538814 17.430796954444602 36-37 17.988278820229596 30.13967251366617 33.763913151201336 18.108135514902894 38-39 16.856612276279265 31.125582576817557 33.84078123962834 18.177023907274833 40-41 16.859468017765845 30.41713775848632 34.89017092073687 17.83322330301096 42-43 16.86909154870128 30.38362152667155 34.29957662877957 18.447710295847596 44-45 16.894610214442558 30.446693041423423 33.95195884576203 18.70673789837199 46-47 17.50362590582314 30.725442491173695 32.956544008696945 18.81438759430622 48-49 16.84003169386569 30.852284843391324 32.98992214514787 19.317761317595114 50-51 16.53866939281522 30.497271238192003 33.081363199712335 19.882696169280443 52-53 16.58035122225491 31.257756502136942 32.888901546048864 19.272990729559282 54-55 16.62724536819336 30.685967167204826 32.543575033700115 20.1432124309017 56-57 16.73502731945018 30.81476234912014 32.18816507994214 20.262045251487546 58-59 16.491132969810813 30.01865135286934 32.66911225774848 20.82110341957136 60-61 16.827427708446262 30.72465448256198 31.75838688553354 20.689530923458214 62-63 16.56605462239284 30.08482010451608 32.068698854166236 21.280426418924847 64-65 16.409372457648804 29.957212784155885 31.89258497653857 21.740829781656743 66-67 16.307336414602013 30.01211307841126 31.203703171000996 22.476847335985727 68-69 16.57306272888562 29.77971389657923 30.990909868034965 22.65631350650018 70-71 16.74370623595815 29.984611311079597 30.833217546775582 22.438464906186674 72-73 16.884227774473075 29.08496680734764 30.809315811254162 23.221489606925125 74-75 16.475621801156297 29.880170838297488 30.15780657582242 23.48640078472379 76-77 16.257264947889816 29.921791631344146 30.63586804081063 23.185075379955403 78-79 16.571564189668152 29.46983600077179 30.041443009400066 23.917156800159997 80-81 16.603145610728976 29.47392660380876 29.70895189996622 24.213975885496055 82-83 16.58323534264715 29.66402596561728 29.61050529776289 24.142233393972685 84-85 16.470975552382587 28.874014397414697 29.43507130435418 25.21993874584853 86-87 16.262658778924703 28.91555035484657 29.95331632659721 24.868474539631517 88-89 16.98153896200359 28.482569042548555 29.68597881670884 24.849913178739012 90-91 16.81484132870808 28.202007322185835 29.91637419434678 25.0667771547593 92-93 16.93555103651002 28.109092741414372 29.68804052207073 25.267315700004882 94-95 16.583578973112928 27.174313791633885 30.200092446436333 26.042014788816854 96-97 17.063912869659358 26.881665397160464 29.99109822426678 26.063323508913395 98-99 16.79433684925744 27.009352847308378 29.765383680342932 26.430926623091246 100-101 16.582669081368774 27.108947766400547 29.948363809547686 26.360019342682996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1522.0 1 1006.5 2 594.0 3 768.0 4 722.5 5 784.0 6 1357.0 7 2365.5 8 3278.5 9 3525.5 10 3188.0 11 2912.5 12 3019.0 13 3141.0 14 3323.5 15 3715.5 16 4116.5 17 4505.5 18 5074.0 19 5786.0 20 6540.5 21 7500.0 22 8564.5 23 9741.5 24 11123.0 25 13581.5 26 18722.5 27 24796.5 28 27431.5 29 30189.5 30 35926.5 31 41686.0 32 50781.5 33 63828.5 34 79089.5 35 90897.5 36 100704.5 37 109527.0 38 108085.0 39 102060.0 40 95408.0 41 88776.5 42 83902.0 43 81622.5 44 81825.0 45 80140.5 46 73917.5 47 64915.0 48 59754.0 49 53626.0 50 43572.0 51 32917.0 52 22890.0 53 15983.0 54 11456.0 55 8371.5 56 6078.5 57 4349.5 58 3046.5 59 2102.0 60 1479.5 61 1020.0 62 686.0 63 485.0 64 370.0 65 321.5 66 311.5 67 273.5 68 221.0 69 168.5 70 121.5 71 89.5 72 69.5 73 47.5 74 26.5 75 20.5 76 14.5 77 8.5 78 8.0 79 7.5 80 6.0 81 5.5 82 3.0 83 1.0 84 1.0 85 0.5 86 2.5 87 3.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025167754571576415 2 0.024488956211762114 3 5.2215258447253975E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.655068091307778E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5664577534176192E-4 26-27 4.6993732602528573E-4 28-29 1.3053814611813493E-4 30-31 5.482602136961667E-4 32-33 0.0027935163269280877 34-35 0.005012664810936381 36-37 0.10604918990637283 38-39 0.09826911639773198 40-41 0.10651912723239812 42-43 0.10717181796298877 44-45 0.10213304552282877 46-47 0.09576278399226379 48-49 0.09633715183518358 50-51 0.09539727718313301 52-53 0.0747722500964677 54-55 0.06312824746273005 56-57 0.03501033078888379 58-59 0.014333088443771217 60-61 0.010181975397214525 62-63 0.001931964562548397 64-65 0.004751588518700112 66-67 0.1653135082440061 68-69 0.4363106995852542 70-71 0.41270940276709545 72-73 0.19486734452515184 74-75 0.030807002483879846 76-77 0.03383548747382058 78-79 0.00571757079997431 80-81 0.001879749304101143 82-83 5.2215258447253975E-5 84-85 7.049059890379286E-4 86-87 0.0013314890904049764 88-89 3.916144383544048E-4 90-91 5.482602136961667E-4 92-93 0.002323579000902802 94-95 0.08398824321240801 96-97 0.018928031187129567 98-99 0.02007676687296915 100-101 0.011121850049265096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1915149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.073744521899428 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03306770330775 20.043523611718378 2 11.69003458524239 6.329860196260421 3 4.5823613567082795 3.721850420356333 4 2.4635516385707454 2.6679027071668435 5 1.4276781943264807 1.9326297346340913 6 0.9748076878339578 1.5835016579040038 7 0.7190009318954315 1.3626233278801165 8 0.4941380952041906 1.0702534838477018 9 0.3893067531227776 0.9485992417226631 >10 2.406443790293673 12.714564482177302 >50 0.3607380517436118 6.941522428288279 >100 0.39144681964645633 21.957169734249497 >500 0.04584858663092602 8.523249179199336 >1k 0.020997882112482928 8.498527631106127 >5k 1.926411202980085E-4 0.39413658004301394 >10k+ 3.85282240596017E-4 1.3100855834458736 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13879 0.7246955719894379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11240 0.5868995049471346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7557 0.3945907080858983 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2993 0.15628026853263113 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2839 0.14823911873175405 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2806 0.14651601520299465 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2753 0.14374860650529017 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2695 0.14072012151534946 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2630 0.13732612971627797 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 0.1333055548158394 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2488 0.1299115630167679 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2476 0.12928497991540083 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2430 0.12688307802682716 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2413 0.12599541863322383 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 2395 0.12505554398117324 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2388 0.12469003717204248 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2293 0.11972958761955335 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 2269 0.11847642141681926 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2226 0.11623116530358735 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2156 0.11257609721227957 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 2130 0.11121850049265096 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2097 0.10949539696389159 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.10892102912097179 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2024 0.10568368309724205 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1937 0.10114095561233095 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1936 0.1010887403538837 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1933 0.10093209457854194 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.2215258447253975E-5 0.0 4 0.0 0.0 0.0 5.2215258447253975E-5 0.0 5 0.0 0.0 0.0 5.2215258447253975E-5 0.0 6 0.0 0.0 0.0 5.2215258447253975E-5 0.0 7 0.0 0.0 0.0 5.2215258447253975E-5 0.0 8 0.0 0.0 0.0 5.2215258447253975E-5 0.0 9 0.0 0.0 0.0 5.2215258447253975E-5 0.0 10-11 0.0 0.0 0.0 5.2215258447253975E-5 0.0 12-13 0.0 0.0 0.0 5.2215258447253975E-5 0.0 14-15 0.0 0.0 0.0 1.827534045653889E-4 0.0 16-17 0.0 0.0 0.0 3.9161443835440484E-4 0.0 18-19 0.0 0.0 0.0 4.699373260252858E-4 0.0 20-21 0.0 0.0 0.0 9.137670228269446E-4 0.0 22-23 0.0 0.0 0.0 0.0022452561132319205 0.0 24-25 0.0 0.0 0.0 0.006866306485813898 0.0 26-27 0.0 0.0 0.0 0.01516853257892728 0.0 28-29 0.0 0.0 0.0 0.03728169453133934 0.0 30-31 0.0 0.0 0.0 0.1228625031263886 0.0 32-33 0.0 0.0 0.0 0.3334205328149402 0.0 34-35 0.0 0.0 0.0 0.6886670436608326 0.0 36-37 0.0 0.0 0.0 1.2420965679432774 0.0 38-39 0.0 0.0 0.0 2.0193467975598764 0.0 40-41 0.0 0.0 0.0 2.8903756313477436 0.0 42-43 0.0 0.0 0.0 3.7078838252271753 0.0 44-45 0.0 0.0 0.0 4.632694375215714 0.0 46-47 0.0 5.2215258447253975E-5 0.0 5.682691007331544 0.0 48-49 0.0 5.2215258447253975E-5 0.0 6.7824487807476075 0.0 50-51 0.0 5.2215258447253975E-5 0.0 7.942854576850156 0.0 52-53 0.0 5.2215258447253975E-5 0.0 8.98099834529846 0.0 54-55 0.0 5.2215258447253975E-5 0.0 10.018854929825304 0.0 56-57 0.0 5.2215258447253975E-5 0.0 11.196700622249235 0.0 58-59 0.0 5.2215258447253975E-5 0.0 12.363215603590113 0.0 60-61 0.0 1.0443051689450795E-4 0.0 13.534221097157454 0.0 62-63 0.0 1.0443051689450795E-4 0.0 14.583695576688811 0.0 64-65 0.0 1.0443051689450795E-4 0.0 15.644318013898658 0.0 66-67 0.0 1.0443051689450795E-4 0.0 16.730264851455424 0.0 68-69 0.0 1.0443051689450795E-4 0.0 17.874170625888638 0.0 70-71 0.0 1.0443051689450795E-4 0.0 19.17970873284533 0.0 72-73 0.0 1.0443051689450795E-4 0.0 20.44514552131453 0.0 74-75 0.0 1.0443051689450795E-4 0.0 21.692072000664176 0.0 76-77 0.0 1.0443051689450795E-4 0.0 22.96983159012693 0.0 78-79 0.0 1.0443051689450795E-4 0.0 24.327872139452335 0.0 80-81 0.0 1.0443051689450795E-4 0.0 25.745751374958292 0.0 82-83 0.0 1.0443051689450795E-4 0.0 27.07478634821625 0.0 84-85 0.0 1.0443051689450795E-4 0.0 28.302915334524887 0.0 86-87 0.0 1.0443051689450795E-4 0.0 29.571145639321013 0.0 88-89 0.0 1.0443051689450795E-4 0.0 30.874908427490496 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 785 0.0 42.297787 9 TTAGGCA 875 0.0 39.031364 4 GCGAGCT 65 1.3394361E-4 36.48765 9 TAGGCAT 945 0.0 36.14015 5 CCTTTAG 915 0.0 34.75029 1 GGCATTC 990 0.0 34.497414 7 GGCGAGC 70 2.0706987E-4 33.88139 8 GTCGCGT 50 1.2305409E-6 33.485268 64-65 TGTCGCG 50 1.2305409E-6 33.485268 64-65 GACATAT 965 0.0 32.949757 1 GGTATCA 11915 0.0 31.226748 1 GTACATG 13425 0.0 31.143425 1 CATGGGT 1755 0.0 30.811794 4 CTTTAGG 1075 0.0 30.453926 2 GACCGGT 110 2.764531E-6 30.185238 6 ACATGGG 13485 0.0 29.582462 3 CATGGGG 4280 0.0 29.479975 4 TACATGG 14135 0.0 29.303608 2 GTATCAA 17525 0.0 29.192108 1 CATATAG 1025 0.0 28.691753 3 >>END_MODULE