FastQCFastQC Report
Fri 27 May 2016
SRR939833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3228210
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92980.2880233937693025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67050.20770024254927652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.14271066628255286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.046.930791
GTATCAA102750.042.6227571
TATCAAC132000.033.609292
ATCAACG131850.033.578683
TCAACGC135250.032.8398134
CAACGCA136800.032.3978465
AACGCAG142650.031.3015946
TACATGG144550.028.0324752
GTACATG145800.026.9785561
ACGCAGA164900.026.6176517
CGCAGAG166000.026.3555138
ACATGGG149050.026.0743833
GCAGAGT178800.023.2476229
CATGGGG66500.023.1198754
GAGTACT91100.021.71847212-13
CATGGGA83550.021.6391724
GTACTTT97750.019.99949314-15
AGAGTAC157850.019.97354910-11
GAGTACA106850.019.8497311
AGTACTT96850.019.62069712-13
Produced by FastQC (version 0.11.5)