FastQCFastQC Report
Fri 27 May 2016
SRR939833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3228210
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104160.3226555893203974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83860.25977244355230916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57810.17907756930311225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.050.818751
GTATCAA107650.041.8315161
ATCAACG136400.032.7607153
TCAACGC137150.032.545684
TATCAAC139600.032.0807652
CAACGCA140150.031.9162855
AACGCAG144400.030.890096
GTACATG143850.027.4693531
TACATGG141350.027.4146392
ACGCAGA166550.026.5839567
CGCAGAG168750.026.1815578
ACATGGG145350.025.6844083
GCAGAGT189800.022.9552469
CATGGGA82000.021.7853534
CATGGGG64550.021.1028354
AGAGTAC168100.020.76284610-11
GAGTACT103550.020.35538712-13
AGTACTT108050.019.87817612-13
TGGTATC31250.019.4601522
GTACTTT106800.019.40518814-15
Produced by FastQC (version 0.11.5)