##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939833_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3228210 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.403852599428166 31.0 31.0 34.0 30.0 34.0 2 31.605056672273488 31.0 31.0 34.0 30.0 34.0 3 31.669472246229333 31.0 31.0 34.0 30.0 34.0 4 35.48142716861666 37.0 35.0 37.0 33.0 37.0 5 35.21410781826461 37.0 35.0 37.0 32.0 37.0 6 35.23899963137466 37.0 35.0 37.0 32.0 37.0 7 35.15434807524913 37.0 35.0 37.0 32.0 37.0 8 35.09523513030441 37.0 35.0 37.0 32.0 37.0 9 36.696937931547204 39.0 35.0 39.0 32.0 39.0 10-11 36.547583335656604 38.0 35.0 39.0 32.0 39.0 12-13 36.488137698600774 38.0 35.0 39.0 32.0 39.0 14-15 37.53016981547049 39.0 36.0 41.0 32.0 41.0 16-17 37.459602999804844 39.0 36.0 41.0 32.0 41.0 18-19 37.43374439704976 39.0 36.0 41.0 32.0 41.0 20-21 37.355501500831735 39.0 36.0 41.0 31.5 41.0 22-23 37.21598424513895 39.0 36.0 40.0 31.0 41.0 24-25 37.05388961684649 39.0 36.0 40.0 31.0 41.0 26-27 36.758224526905 39.0 35.5 40.0 30.0 41.0 28-29 36.534781039647356 38.0 35.0 40.0 30.0 41.0 30-31 36.28281028805437 38.0 35.0 40.0 30.0 41.0 32-33 36.25358743700069 38.0 35.0 40.0 30.0 41.0 34-35 36.338702407835925 38.0 35.0 40.0 30.0 41.0 36-37 36.31661214728905 38.0 35.0 40.0 30.0 41.0 38-39 36.18452036887315 38.0 35.0 40.0 30.0 41.0 40-41 36.01285046511844 38.0 35.0 40.0 29.5 41.0 42-43 35.809392821408764 38.0 35.0 40.0 29.0 41.0 44-45 35.55882563402009 38.0 34.0 40.0 27.5 41.0 46-47 35.2613106024701 38.0 34.0 40.0 27.0 41.0 48-49 34.94924462782781 38.0 34.0 40.0 26.0 41.0 50-51 34.683904392836894 38.0 33.0 40.0 25.5 41.0 52-53 34.4033057019215 38.0 33.0 40.0 24.0 41.0 54-55 34.089985936478726 37.0 33.0 40.0 24.0 41.0 56-57 32.8404948872595 36.5 31.5 39.5 16.0 41.0 58-59 29.65109193639819 34.5 25.5 39.0 8.0 40.0 60-61 29.656087274371863 34.0 25.0 38.0 9.5 40.0 62-63 31.370077535228504 35.0 29.5 38.0 12.5 40.0 64-65 30.872096455930688 35.0 29.0 38.0 6.5 40.0 66-67 25.442833489766777 28.5 18.0 36.0 2.0 39.0 68-69 17.718548204732656 13.5 7.0 29.5 2.0 35.5 70-71 16.563463033693594 15.0 7.5 26.0 2.0 33.5 72-73 18.761021278045728 19.5 7.5 30.0 2.0 34.5 74-75 19.501652153980068 20.0 8.5 31.0 2.0 35.0 76-77 20.53563120119199 23.5 9.5 31.5 2.0 34.5 78-79 23.710744344388996 29.0 13.0 34.0 2.0 36.0 80-81 26.312770544667167 31.5 23.0 35.0 2.0 36.0 82-83 27.16644998311758 33.0 26.0 35.0 2.0 36.0 84-85 27.11669098354816 33.0 26.0 35.0 2.0 36.0 86-87 26.80820996775303 33.0 25.5 35.0 2.0 35.5 88-89 26.35550490829283 33.0 25.0 35.0 2.0 35.0 90-91 24.847069428568773 31.5 14.5 34.5 2.0 35.0 92-93 22.852352542120865 31.0 2.0 34.0 2.0 35.0 94-95 18.370689948919058 24.0 2.0 33.0 2.0 35.0 96-97 17.038277094736713 15.5 2.0 32.5 2.0 34.5 98-99 15.917430092837828 6.0 2.0 32.0 2.0 34.0 100-101 15.27117737074106 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5046320088221208 1101 2 0.45785057153184283 1101 3 0.4408557779568234 1101 4 0.3201294223089519 1101 5 0.42564913162006945 1101 6 0.3704308645276768 1101 7 0.39794870820238515 1101 8 0.5181815309085707 1101 9 0.5437052696905482 1101 10-11 0.5197477570831808 1101 12-13 0.591127908320054 1101 14-15 0.42906524413288594 1101 16-17 0.3407652048081786 1101 18-19 0.527023931319242 1101 20-21 0.6044773740353975 1101 22-23 0.5925372237374731 1101 24-25 0.5343621889389283 1101 26-27 0.4233707075902515 1101 28-29 0.48013228602123803 1101 30-31 0.49656556200320523 1101 32-33 0.39771068774104634 1101 34-35 0.4317189709272853 1101 36-37 0.40039530668546064 1101 38-39 0.3491101385984692 1101 40-41 0.37365856097129324 1101 42-43 0.43688643816655315 1101 44-45 0.31794980265242145 1101 46-47 -0.07457584535610096 1101 48-49 -0.12104619203244482 1101 50-51 -0.15111632366457428 1101 52-53 -0.09847951432312385 1101 54-55 -0.008778170608458424 1101 56-57 0.6769630981752428 1101 58-59 -8.618144365116148 1101 60-61 -9.893745973452035 1101 62-63 -2.928813207389833 1101 64-65 -0.4368672464276564 1101 66-67 -1.7217925581559115 1101 68-69 -4.112720677662583 1101 70-71 -4.375470272168732 1101 72-73 -7.242119729385928 1101 74-75 -7.963058666600826 1101 76-77 -9.1964926237582 1101 78-79 -12.301887964893307 1101 80-81 -8.58962164428753 1101 82-83 -5.045603961509993 1101 84-85 -4.150442347626768 1101 86-87 -4.011277317440477 1101 88-89 -4.1964934760279995 1101 90-91 -10.013637827219382 1101 92-93 -15.451086887107277 1101 94-95 -13.26786751964579 1101 96-97 -12.657057024198812 1101 98-99 -12.05939940494926 1101 100-101 -11.750841417753438 1102 1 0.5037885690916433 1102 2 0.47740474667597965 1102 3 0.45650287713500504 1102 4 0.35571717528213753 1102 5 0.4161680702486663 1102 6 0.44917618331970033 1102 7 0.4834333218881923 1102 8 0.61521586524902 1102 9 0.7773322014964492 1102 10-11 0.6684803655438927 1102 12-13 0.7105909261896954 1102 14-15 0.6867936424431846 1102 16-17 0.8390959459544121 1102 18-19 0.7956782807899145 1102 20-21 0.7367946248447694 1102 22-23 0.8072719278757319 1102 24-25 0.8280977106733616 1102 26-27 0.9493840099739685 1102 28-29 0.6862035249613712 1102 30-31 0.7438654976292511 1102 32-33 0.49861861889381487 1102 34-35 0.5833319613087866 1102 36-37 0.598686460200895 1102 38-39 0.47042684434372717 1102 40-41 0.6554584707537785 1102 42-43 0.5829941635106195 1102 44-45 0.4249550512439484 1102 46-47 0.30403520440919607 1102 48-49 0.4879824896172167 1102 50-51 0.338152726561205 1102 52-53 0.372080562549705 1102 54-55 0.2866260226261659 1102 56-57 1.015541890482318 1102 58-59 -9.325534800180687 1102 60-61 -10.216570180877127 1102 62-63 -3.478645459209453 1102 64-65 -0.5987882920109904 1102 66-67 -1.5831318887607893 1102 68-69 -4.408039704629161 1102 70-71 -4.515622232285075 1102 72-73 -7.157142001833407 1102 74-75 -8.15868323489595 1102 76-77 -8.735297899602932 1102 78-79 -12.109283568669287 1102 80-81 -8.249390626380293 1102 82-83 -4.22523078054401 1102 84-85 -3.211644589907767 1102 86-87 -2.9060364789815054 1102 88-89 -2.9974916246895376 1102 90-91 -9.001350977830935 1102 92-93 -15.030727332744178 1102 94-95 -12.752270932874874 1102 96-97 -12.442371070407091 1102 98-99 -11.953451980142265 1102 100-101 -11.595468777152742 1103 1 0.22133614226432385 1103 2 0.2035358438968231 1103 3 0.23070345787295565 1103 4 0.10908159588597499 1103 5 0.13802459239420273 1103 6 0.1230049832183866 1103 7 0.14420320673563936 1103 8 0.2735822909968775 1103 9 0.2542315854800208 1103 10-11 0.2605289141512941 1103 12-13 0.2080163514159139 1103 14-15 0.38453808156067737 1103 16-17 0.3589633049234351 1103 18-19 0.35600070191840416 1103 20-21 0.3651967361257462 1103 22-23 0.2742845195771437 1103 24-25 0.3110583585911755 1103 26-27 0.24576743874009566 1103 28-29 0.2855370784082609 1103 30-31 0.33076987185847884 1103 32-33 0.23241517064914063 1103 34-35 0.3936093797687832 1103 36-37 0.14146846938535163 1103 38-39 0.10318712862189017 1103 40-41 0.2867193445141396 1103 42-43 0.2649389722982818 1103 44-45 0.2437859165917402 1103 46-47 0.23011119786006162 1103 48-49 0.19562517898271636 1103 50-51 0.20798390248017284 1103 52-53 0.1374591880159457 1103 54-55 0.09124501510913063 1103 56-57 0.5954106967733495 1103 58-59 -6.819665381793065 1103 60-61 -8.687961976973575 1103 62-63 -2.456888591214266 1103 64-65 -0.39824034494679594 1103 66-67 -1.9876142902298248 1103 68-69 -4.555985562725269 1103 70-71 -4.975689864436127 1103 72-73 -7.750071758472242 1103 74-75 -8.321551359739534 1103 76-77 -9.228479505346389 1103 78-79 -12.320219970928315 1103 80-81 -8.743513820619953 1103 82-83 -4.883230852910106 1103 84-85 -3.9202673122601475 1103 86-87 -3.6823916095872384 1103 88-89 -3.786787397001664 1103 90-91 -9.423064501547415 1103 92-93 -15.065052081648888 1103 94-95 -12.651500083146095 1103 96-97 -12.44517148771827 1103 98-99 -12.044920092321782 1103 100-101 -11.736006758801905 1104 1 0.14874966742533502 1104 2 0.18363699672675793 1104 3 0.17033113687391932 1104 4 0.16814064284294972 1104 5 0.035610626452680094 1104 6 0.12249317407886906 1104 7 0.11917090382119255 1104 8 0.2187819393799728 1104 9 0.11786794911638765 1104 10-11 0.18020296326179874 1104 12-13 0.08991211060367021 1104 14-15 0.20664687626526757 1104 16-17 0.31397774993040173 1104 18-19 0.3181798451922191 1104 20-21 0.2925770138426529 1104 22-23 0.3389002975043738 1104 24-25 0.2451174685167956 1104 26-27 0.3133140292398906 1104 28-29 0.24081246547651602 1104 30-31 0.24738100018101505 1104 32-33 0.2233541086955526 1104 34-35 0.18311074366960867 1104 36-37 0.18069768280624032 1104 38-39 0.3050401698814582 1104 40-41 0.2363385101544182 1104 42-43 0.12339477964445678 1104 44-45 -0.020342232155307727 1104 46-47 0.06480389828296751 1104 48-49 0.17319877043711784 1104 50-51 -0.03023554576337517 1104 52-53 0.17934252978707121 1104 54-55 0.021750552571702997 1104 56-57 0.6016559268985517 1104 58-59 -6.48552428669278 1104 60-61 -8.917949408432207 1104 62-63 -2.770477224618375 1104 64-65 -0.7291254573962007 1104 66-67 -1.7839434701513923 1104 68-69 -4.5431703102084136 1104 70-71 -5.02423818531426 1104 72-73 -8.031765136437752 1104 74-75 -8.991945392327066 1104 76-77 -9.213198148850669 1104 78-79 -12.385273632806847 1104 80-81 -8.792919900368481 1104 82-83 -5.0472446505994135 1104 84-85 -4.102326915859937 1104 86-87 -3.789842185498461 1104 88-89 -3.881067073493959 1104 90-91 -9.43307626409014 1104 92-93 -14.888757383356849 1104 94-95 -12.493969914868691 1104 96-97 -12.219500682752312 1104 98-99 -11.655454620548106 1104 100-101 -11.1804936021334 1105 1 0.1860087425819863 1105 2 0.24402252315002215 1105 3 0.15856464640123136 1105 4 0.18644801381878295 1105 5 0.19769134896466767 1105 6 0.14006333078648936 1105 7 0.21924231488991097 1105 8 0.2225816616702616 1105 9 0.25668056507186066 1105 10-11 0.27304622063336126 1105 12-13 0.2510242329826369 1105 14-15 0.3646459661242005 1105 16-17 0.3982821337765614 1105 18-19 0.32504382577587876 1105 20-21 0.4536045397758386 1105 22-23 0.377675691023704 1105 24-25 0.27963188491800395 1105 26-27 0.2517912932158026 1105 28-29 0.30201105125731686 1105 30-31 0.3341955064294879 1105 32-33 0.30784985553631117 1105 34-35 0.37939888499818153 1105 36-37 0.245169509272678 1105 38-39 0.31236750561850357 1105 40-41 0.33742029449765454 1105 42-43 0.2520164993443359 1105 44-45 0.37620133209714623 1105 46-47 0.41937128956194414 1105 48-49 0.485433983561812 1105 50-51 0.4871193275753214 1105 52-53 0.44635726707135603 1105 54-55 0.42514234496618997 1105 56-57 1.2318633923890374 1105 58-59 -6.757872100603937 1105 60-61 -8.694533345609138 1105 62-63 -2.460403829908863 1105 64-65 0.08188911652942821 1105 66-67 -1.2719009035535684 1105 68-69 -3.9415769799088824 1105 70-71 -4.90177485349783 1105 72-73 -6.436222377310086 1105 74-75 -6.770839920979007 1105 76-77 -9.015680500281807 1105 78-79 -12.164237472201952 1105 80-81 -8.10745520334044 1105 82-83 -4.05925081155436 1105 84-85 -3.160633820096759 1105 86-87 -3.0132723671766186 1105 88-89 -3.0193889133791423 1105 90-91 -8.3779945264208 1105 92-93 -11.548621562304586 1105 94-95 -11.601569009395009 1105 96-97 -11.80815869141032 1105 98-99 -11.358542915900552 1105 100-101 -10.952511898302639 1106 1 0.20641690584729133 1106 2 0.17790007417043086 1106 3 0.15529934027878056 1106 4 0.17461127912491037 1106 5 0.09483420610752802 1106 6 0.03802251445996063 1106 7 0.18005864142052275 1106 8 0.18380615146617885 1106 9 0.2530070956841044 1106 10-11 0.21141356757213003 1106 12-13 0.19592219216631435 1106 14-15 0.19342147632828244 1106 16-17 0.1607311133683993 1106 18-19 0.2534111727146495 1106 20-21 0.23646168263297795 1106 22-23 0.28134916041145885 1106 24-25 0.23350943593841578 1106 26-27 0.31485251770560296 1106 28-29 0.2981335002369079 1106 30-31 0.38664448602131785 1106 32-33 0.32825801880161976 1106 34-35 0.4167458237736881 1106 36-37 0.18394501947675934 1106 38-39 0.35216342398585 1106 40-41 0.27068560062010505 1106 42-43 0.2679348666912773 1106 44-45 0.25926255658694686 1106 46-47 0.16610598343949334 1106 48-49 0.19890337131691638 1106 50-51 0.230588715330434 1106 52-53 0.27125522625502896 1106 54-55 -0.01689847136034217 1106 56-57 0.9096184944298571 1106 58-59 -6.6935863863182234 1106 60-61 -8.906778243568318 1106 62-63 -2.6665262788884547 1106 64-65 -0.16872312836853354 1106 66-67 -1.4806764137576494 1106 68-69 -4.147699428888474 1106 70-71 -4.753611588191708 1106 72-73 -6.292140744657026 1106 74-75 -6.853288900570844 1106 76-77 -8.679966214567521 1106 78-79 -12.13076808444685 1106 80-81 -8.114189897217994 1106 82-83 -4.026801831962523 1106 84-85 -3.015531779280437 1106 86-87 -2.8073539998296795 1106 88-89 -2.9753072807260814 1106 90-91 -8.70860677131876 1106 92-93 -12.419846052100503 1106 94-95 -11.907283295109295 1106 96-97 -12.19754644651236 1106 98-99 -11.70242046692096 1106 100-101 -11.465573122792435 1107 1 0.14862824815655173 1107 2 0.0747505058975868 1107 3 0.07171629629007015 1107 4 0.014805951853240629 1107 5 0.03716528296912003 1107 6 0.07175951476803988 1107 7 0.15828610376409102 1107 8 0.0788222631740183 1107 9 -0.02157420807363053 1107 10-11 0.16538928760311222 1107 12-13 0.27416322664620196 1107 14-15 0.18249773841206007 1107 16-17 0.20411290672221583 1107 18-19 0.16253206464547532 1107 20-21 0.24313417782444446 1107 22-23 0.13506201397392914 1107 24-25 0.017745191440127428 1107 26-27 0.310107265871693 1107 28-29 0.26921127773713494 1107 30-31 0.2684421695990409 1107 32-33 0.2570094655247388 1107 34-35 0.04473406680651948 1107 36-37 0.1908668090004042 1107 38-39 0.27542681502157507 1107 40-41 0.16080400293818542 1107 42-43 0.20464316567795038 1107 44-45 0.20974640741001593 1107 46-47 0.12940034892130114 1107 48-49 0.03358900566858125 1107 50-51 0.26406026796617255 1107 52-53 0.17977869439381067 1107 54-55 0.13973264130504504 1107 56-57 0.7841819795546243 1107 58-59 -6.670727161845079 1107 60-61 -9.171902182984969 1107 62-63 -2.7737611434585183 1107 64-65 -0.3512721920576567 1107 66-67 -1.6224320486921613 1107 68-69 -4.058581284690771 1107 70-71 -4.654597691323293 1107 72-73 -6.205559674040284 1107 74-75 -6.870988881419976 1107 76-77 -9.143648176948556 1107 78-79 -12.390055172599123 1107 80-81 -8.339320081482644 1107 82-83 -4.190056979798889 1107 84-85 -3.2867398659422733 1107 86-87 -3.0636428115600047 1107 88-89 -3.188502061698447 1107 90-91 -8.585556121438247 1107 92-93 -11.881195272327151 1107 94-95 -11.769508376084158 1107 96-97 -12.467915059073665 1107 98-99 -11.758563898589834 1107 100-101 -11.32348701050174 1108 1 -0.03977156110066815 1108 2 0.018786437154442837 1108 3 -0.00435047745422068 1108 4 0.042527694636262936 1108 5 -0.11161201795171394 1108 6 0.027269622585912145 1108 7 0.0884239791167829 1108 8 -0.12439040985877625 1108 9 0.07167181051518412 1108 10-11 0.05583304000246159 1108 12-13 0.032770838797965496 1108 14-15 0.07236375366115055 1108 16-17 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4.702304945982068 2311 60-61 4.270945976976115 2311 62-63 1.7625330993054718 2311 64-65 1.602229362482415 2311 66-67 -0.12486849446715453 2311 68-69 -3.253592189870089 2311 70-71 -0.455041478403043 2311 72-73 -0.8471493716942717 2311 74-75 1.3285437678761802 2311 76-77 4.548118033671354 2311 78-79 5.477742020726076 2311 80-81 3.3274581416421363 2311 82-83 1.8831614619172257 2311 84-85 1.6734801227340057 2311 86-87 1.5940619332640757 2311 88-89 1.7632290902035521 2311 90-91 3.198309914580154 2311 92-93 5.169569507338132 2311 94-95 9.13418593878782 2311 96-97 9.873060633153107 2311 98-99 10.180739336580407 2311 100-101 9.444461715052233 2312 1 -0.29199493802888554 2312 2 -0.2556890707742241 2312 3 -0.3457724316332147 2312 4 -0.18765366992334975 2312 5 -0.3074164599731688 2312 6 -0.27258973043799983 2312 7 -0.27379858383835654 2312 8 -0.29805667711074335 2312 9 -0.3320402853140578 2312 10-11 -0.26744791510976285 2312 12-13 -0.3071730917076678 2312 14-15 -0.47527657624402764 2312 16-17 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2312 82-83 1.885571063359805 2312 84-85 1.4951705581821422 2312 86-87 1.2167899834702318 2312 88-89 1.4530597915402446 2312 90-91 2.9239331248550506 2312 92-93 4.868666047221055 2312 94-95 8.972852118410085 2312 96-97 9.586723062098619 2312 98-99 10.03242420580681 2312 100-101 9.094890524308399 2313 1 -0.24667959468293077 2313 2 -0.2525083055123005 2313 3 -0.28707021411774036 2313 4 -0.310207687698302 2313 5 -0.3163835747481869 2313 6 -0.2532366021241046 2313 7 -0.27085745354344226 2313 8 -0.334779344237937 2313 9 -0.2860653392283581 2313 10-11 -0.3977816842295354 2313 12-13 -0.3275875574387257 2313 14-15 -0.5227333270564856 2313 16-17 -0.5016121463108689 2313 18-19 -0.5184566178798491 2313 20-21 -0.3484095489985961 2313 22-23 -0.36932162540021807 2313 24-25 -0.4182805525914617 2313 26-27 -0.49484244193254767 2313 28-29 -0.5659761584728145 2313 30-31 -0.5125458963882679 2313 32-33 -0.40891849125097224 2313 34-35 -0.44610655790008025 2313 36-37 -0.6874402684666236 2313 38-39 -0.8571813722571449 2313 40-41 -0.9435960017345124 2313 42-43 -0.7615354516587587 2313 44-45 -0.6554553432983496 2313 46-47 -0.6522674231872045 2313 48-49 -0.7998358889683175 2313 50-51 -0.5325975984315576 2313 52-53 -0.36150485338658456 2313 54-55 -0.40513358725187487 2313 56-57 0.7738403655749195 2313 58-59 4.077612458012727 2313 60-61 3.833963743576483 2313 62-63 1.2113190692189733 2313 64-65 1.122636297692452 2313 66-67 1.9369463490272096 2313 68-69 0.37299819268401535 2313 70-71 2.7398950233606527 2313 72-73 8.25382685765431 2313 74-75 9.896083488893957 2313 76-77 8.818841974667876 2313 78-79 6.523201335770864 2313 80-81 3.4836955963219474 2313 82-83 1.9127735570477995 2313 84-85 1.6183735813792062 2313 86-87 1.4570558298498035 2313 88-89 1.5821106429651373 2313 90-91 3.070932692398859 2313 92-93 4.985670103460343 2313 94-95 8.72973252500454 2313 96-97 9.599689361914935 2313 98-99 10.102939435258362 2313 100-101 9.310155121845822 2314 1 -0.3659579610323682 2314 2 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3.763663498623764 2314 62-63 1.3257674117507392 2314 64-65 1.1938572328494352 2314 66-67 1.958051499132747 2314 68-69 -0.19010776602524615 2314 70-71 2.4882888269264143 2314 72-73 8.3770622720129 2314 74-75 9.837007984956529 2314 76-77 8.917679239780352 2314 78-79 6.620689610452196 2314 80-81 3.58733549888294 2314 82-83 1.9640906720039233 2314 84-85 1.6711794419492705 2314 86-87 1.416281761183992 2314 88-89 1.6381716699596254 2314 90-91 2.8450057343863975 2314 92-93 4.940211977501114 2314 94-95 8.848398933053534 2314 96-97 9.575070427713369 2314 98-99 9.834863443448297 2314 100-101 8.907163288065837 2315 1 -0.7125377021306996 2315 2 -0.5751167969521589 2315 3 -0.6198088763228533 2315 4 -0.42791573993581267 2315 5 -0.6695781117769855 2315 6 -0.7130298954999006 2315 7 -0.7065769591745976 2315 8 -0.7993794308823041 2315 9 -1.093498813419565 2315 10-11 -0.8857059900170654 2315 12-13 -0.687847001893708 2315 14-15 -0.8843740490345482 2315 16-17 -1.0209472966476056 2315 18-19 -0.9640474916314119 2315 20-21 -0.8829802509492524 2315 22-23 -0.9903174594689617 2315 24-25 -0.9442686849385495 2315 26-27 -1.011358076573373 2315 28-29 -1.0557083996592311 2315 30-31 -1.0134779068147637 2315 32-33 -0.7866778372464438 2315 34-35 -0.8767802689192266 2315 36-37 -1.1178740300137449 2315 38-39 -1.0892376371263524 2315 40-41 -0.8052313631392636 2315 42-43 -0.7565855274361155 2315 44-45 -0.8467723085282941 2315 46-47 -0.7787714833656594 2315 48-49 -0.7343372316642984 2315 50-51 -0.7791914828561346 2315 52-53 -0.8532676943299933 2315 54-55 -0.7423769543628964 2315 56-57 0.588792062068741 2315 58-59 3.981277886763774 2315 60-61 3.6657804965714718 2315 62-63 1.0665703160081037 2315 64-65 1.0700967352027533 2315 66-67 1.9129886341046856 2315 68-69 6.0844483408108765 2315 70-71 5.007441383203176 2315 72-73 8.996605290208091 2315 74-75 10.230770555398614 2315 76-77 8.969106575483853 2315 78-79 6.342411041398812 2315 80-81 3.5104354852596167 2315 82-83 1.7399202296269003 2315 84-85 1.257574360011592 2315 86-87 1.1866776651058935 2315 88-89 1.2820837306991244 2315 90-91 2.533377901504977 2315 92-93 4.512114759734544 2315 94-95 8.559373359299375 2315 96-97 9.105600396819197 2315 98-99 9.397731401254097 2315 100-101 8.664929642275078 2316 1 -0.469444030369516 2316 2 -0.41174377460916034 2316 3 -0.6027173862585364 2316 4 -0.42228911585377915 2316 5 -0.5812885222063571 2316 6 -0.5816507761538503 2316 7 -0.6133936510760023 2316 8 -0.7919832751722566 2316 9 -0.8180377821630103 2316 10-11 -0.7100287471035287 2316 12-13 -0.6536981366660157 2316 14-15 -0.8115662608963703 2316 16-17 -0.938360882586835 2316 18-19 -0.9121696470756149 2316 20-21 -0.7533533421998371 2316 22-23 -0.8956025203429689 2316 24-25 -2.281494782009375 2316 26-27 -1.5092992837212336 2316 28-29 -1.1147342573232777 2316 30-31 -0.6523083455903773 2316 32-33 -0.7608839221247621 2316 34-35 -0.8872186002110638 2316 36-37 -0.6788353623173506 2316 38-39 -1.0416111644150803 2316 40-41 -0.8495483139147737 2316 42-43 -1.0843604790407753 2316 44-45 -0.9764314494578485 2316 46-47 -1.0279834705737017 2316 48-49 -1.1385367533779132 2316 50-51 -1.091708865075951 2316 52-53 -0.9289644422310417 2316 54-55 -0.8292069458040459 2316 56-57 0.18891982350240966 2316 58-59 3.809188017722068 2316 60-61 3.7335209793236857 2316 62-63 1.0644669779817733 2316 64-65 0.829746790121721 2316 66-67 5.832216372154964 2316 68-69 8.889686672554568 2316 70-71 5.608343695975066 2316 72-73 9.402322849704891 2316 74-75 10.321271287588736 2316 76-77 8.890293510455802 2316 78-79 6.120060923860553 2316 80-81 3.203280619047643 2316 82-83 1.7480528304061203 2316 84-85 1.401819014308284 2316 86-87 1.3535699821364986 2316 88-89 1.5242517062960523 2316 90-91 2.8240685249152513 2316 92-93 4.733280871814422 2316 94-95 8.93247876794204 2316 96-97 9.626657311092513 2316 98-99 9.941876309002657 2316 100-101 9.05959322077757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 26.0 4 59.0 5 148.0 6 322.0 7 865.0 8 1883.0 9 3209.0 10 5218.0 11 7991.0 12 11450.0 13 15435.0 14 20222.0 15 28203.0 16 37350.0 17 48180.0 18 51909.0 19 53706.0 20 55617.0 21 55399.0 22 53150.0 23 51718.0 24 54375.0 25 59871.0 26 78989.0 27 112461.0 28 155230.0 29 202419.0 30 241006.0 31 260641.0 32 274862.0 33 288444.0 34 292059.0 35 281606.0 36 234815.0 37 143350.0 38 44102.0 39 1915.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71015487803215 19.556124541915718 14.140567584549624 23.5931529955025 2 15.945915218601487 20.690771467418497 39.405237166915065 23.958076147064954 3 19.59411255025035 24.68604470697351 29.66022910782764 26.0596136349485 4 13.09193020280589 16.58532127711642 36.52119905458443 33.80154946549327 5 13.39040867660939 38.03400033166519 34.22059454860212 14.354996443123294 6 32.63000173161139 36.172587445600605 17.060616001390244 14.13679482139776 7 29.005702850805864 32.51198032346099 20.336904972105284 18.145411853627863 8 26.581451121768563 35.69487201250478 19.215378448587234 18.508298417139425 9 26.57968347784066 16.223882585085853 19.73217355748232 37.46426037959117 10-11 25.32728663872549 26.72529048605884 27.125171534689503 20.822251340526172 12-13 25.85242285972722 23.81181831417411 27.808042227736113 22.52771659836256 14-15 22.67834952835237 25.256779736227987 25.860722268369308 26.204148467050338 16-17 22.201560617184136 28.540197199066974 26.57122677892702 22.687015404821867 18-19 22.165160265072988 27.064549779402707 28.829715310621275 21.94057464490303 20-21 23.034545460177622 26.266181568113602 28.860359146400015 21.83891382530876 22-23 22.602293531089984 26.194392558104955 28.77780565700497 22.425508253800093 24-25 22.348406702166216 26.413089606933877 28.98214490383215 22.256358787067757 26-27 22.20647389830183 26.700636993974523 28.751851452020077 22.341037655703573 28-29 22.102325345101498 26.834904695251403 28.818923688205405 22.24384627144169 30-31 22.32119110872363 26.6146894136078 28.71822228996386 22.345897187704704 32-33 22.15889316664191 26.599617960532502 28.650574832635083 22.5909140401905 34-35 22.442884367329288 26.736732804904793 28.6538454389893 22.166537388776614 36-37 22.55925285177612 26.40574812346152 28.667044574479572 22.367954450282788 38-39 22.19198692967808 26.728318777789813 28.672290586000248 22.407403706531863 40-41 22.390018446774462 26.585865652886763 28.69160516063505 22.332510739703725 42-43 22.23152285492345 26.496562690653935 28.65170158199579 22.62021287242682 44-45 22.206139233103244 26.711574407235638 28.568325634826635 22.51396072483448 46-47 22.381312699392435 26.85156093135323 28.15202720289133 22.615099166363006 48-49 22.234914270575604 26.831504924387378 28.209093965398374 22.724486839638647 50-51 22.21181152485887 27.03757254761074 28.052445127336618 22.698170800193775 52-53 22.4296420274597 26.960192293511582 27.935385364698973 22.674780314329745 54-55 22.198948305363842 26.980757142064903 27.97628249832778 22.844012054243475 56-57 22.15891392056631 27.0443415626369 28.032283045351146 22.764461471445642 58-59 20.72353541114692 26.636319562097615 29.80141236879427 22.83873265796119 60-61 22.108480189986935 26.770783045058344 28.32283297318706 22.797903791767656 62-63 21.943308274962238 26.914893023843657 28.146680065274847 22.99511863591926 64-65 21.348470933521362 26.869111078056118 28.585911118507983 23.196506869914533 66-67 21.50425909530486 26.62395785502387 28.906423104810237 22.965359944861035 68-69 22.195474839834255 25.850729219227027 29.4022963461313 22.55149959480742 70-71 21.259014396896593 26.147489576701368 29.608884916549457 22.984611109852594 72-73 21.127691440239783 26.342271073280166 29.506854210615046 23.02318327586501 74-75 21.645070493466065 26.556848414457672 28.737082230704885 23.060998861371374 76-77 20.100498654696157 26.88501956812715 29.395104450822572 23.619377326354122 78-79 21.15064733323782 27.105098879963464 28.166909882334018 23.577343904464698 80-81 21.693381473627667 27.159672554719677 27.405831137968846 23.741114833683813 82-83 21.87568633342678 27.180987576687354 27.23752054160129 23.705805548284573 84-85 21.857153898207006 27.047648873980723 27.141973636461213 23.953223591351055 86-87 21.75355125044008 27.01117228011877 27.258545095935766 23.97673137350538 88-89 21.848623137046932 27.002132452431816 27.079590286623418 24.06965412389784 90-91 21.485722819612334 27.084804902183297 27.361400336379294 24.06807194182507 92-93 21.30872925803898 26.966853515384376 27.506561117778 24.217856108798646 94-95 20.11132678120339 26.76679631982563 28.71132690675519 24.41054999221579 96-97 19.803676461243267 26.707840153940847 28.899997285574937 24.58848609924095 98-99 20.198157148532275 26.740763329349264 28.26020595838931 24.80087356372914 100-101 20.10789150444994 26.86901883645636 28.220469805650968 24.802619853442735 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 468.0 1 346.0 2 241.5 3 312.5 4 359.0 5 397.0 6 576.0 7 889.5 8 1254.5 9 1601.5 10 1863.0 11 2073.5 12 2363.5 13 2660.0 14 2970.0 15 3283.5 16 3520.0 17 3835.5 18 4277.5 19 4750.0 20 5381.5 21 6260.5 22 7435.0 23 8865.5 24 10677.0 25 13209.5 26 16604.5 27 20585.5 28 24841.5 29 29379.5 30 34492.5 31 40611.5 32 47855.0 33 55229.5 34 61986.0 35 69231.0 36 77164.0 37 83990.5 38 89893.5 39 95197.5 40 100200.5 41 105305.5 42 110039.0 43 114771.0 44 118986.0 45 122417.5 46 125500.5 47 127672.5 48 129156.0 49 128903.5 50 126790.5 51 124325.0 52 120774.5 53 116105.5 54 110606.5 55 102633.0 56 92595.5 57 82758.0 58 72362.0 59 61421.0 60 50221.0 61 39585.0 62 30334.0 63 22336.5 64 16195.0 65 11673.5 66 8329.5 67 5994.0 68 4214.5 69 2827.0 70 1961.0 71 1389.0 72 994.0 73 734.5 74 522.0 75 355.0 76 222.5 77 118.0 78 68.0 79 43.5 80 24.5 81 15.5 82 12.5 83 7.0 84 4.0 85 3.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01976327438425629 2 0.0051731454892959255 3 0.0038101610490023884 4 0.0 5 0.06396733793650351 6 9.293075729274118E-5 7 0.0 8 1.2390767639032158E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7037305503669217E-4 16-17 0.0 18-19 0.0013165190616471667 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.5488459548790197E-5 28-29 0.002028988200891516 30-31 0.008038510505822112 32-33 2.9428073142701374E-4 34-35 6.814922201467687E-4 36-37 0.024518231465734883 38-39 0.023093293187246185 40-41 0.025292654443174394 42-43 0.025741819770089307 44-45 0.026407823530687283 46-47 0.028065088702407835 48-49 0.028034111783310257 50-51 0.023557946973709887 52-53 0.01994913589884177 54-55 0.014466221218570042 56-57 0.15779642588307452 58-59 0.7725333853745574 60-61 0.3192016628410172 62-63 4.3367686736612546E-4 64-65 0.1432837392858581 66-67 1.4382893306197553 68-69 2.755768676758947 70-71 2.2284640714203845 72-73 1.8793077278120074 74-75 1.5842370849480054 76-77 1.1437762722995097 78-79 0.43319672512011304 80-81 2.0134997413427257E-4 82-83 1.5488459548790195E-4 84-85 4.646537864637059E-5 86-87 0.002679503501940704 88-89 6.195383819516079E-5 90-91 0.23049925500509572 92-93 0.18019273839062513 94-95 1.3095182779311134 96-97 1.2865179155011601 98-99 1.0087478819531566 100-101 0.40954584738911043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3228210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.83948899518295 #Duplication Level Percentage of deduplicated Percentage of total 1 72.24869142677117 36.730865527077015 2 14.444550165110293 14.687070983189857 3 5.479589445564192 8.357385819476441 4 2.552115544566664 5.18993000569729 5 1.4592825782889018 3.709459028989041 6 0.8651108576567454 2.638907635647204 7 0.585520203659076 2.083728356526801 8 0.40508753282146687 1.6475554533570218 9 0.29707076090848145 1.3592633111997632 >10 1.509820432071007 13.74426737333802 >50 0.1010983021601638 3.5205078325415364 >100 0.04882404049460426 4.378879328868264 >500 0.0025665248486599094 0.8510455613283374 >1k 4.8886187532122E-4 0.3374171655172334 >5k 1.22215468830305E-4 0.44012420439026295 >10k+ 6.11077344151525E-5 0.3235924128558607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10416 0.3226555893203974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8386 0.25977244355230916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5781 0.17907756930311225 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0976919097580395E-5 2 0.0 0.0 0.0 0.0 3.0976919097580395E-5 3 0.0 0.0 0.0 3.0976919097580395E-5 3.0976919097580395E-5 4 0.0 0.0 0.0 3.0976919097580395E-5 3.0976919097580395E-5 5 0.0 0.0 0.0 3.0976919097580395E-5 3.0976919097580395E-5 6 0.0 0.0 0.0 3.0976919097580395E-5 3.0976919097580395E-5 7 0.0 0.0 0.0 3.0976919097580395E-5 3.0976919097580395E-5 8 0.0 0.0 0.0 3.0976919097580395E-5 3.0976919097580395E-5 9 0.0 0.0 0.0 6.195383819516079E-5 3.0976919097580395E-5 10-11 0.0 0.0 0.0 2.0134997413427254E-4 3.0976919097580395E-5 12-13 3.0976919097580395E-5 0.0 0.0 3.4074611007338433E-4 4.646537864637059E-5 14-15 1.2390767639032158E-4 0.0 0.0 3.717230291709647E-4 7.744229774395099E-5 16-17 1.2390767639032158E-4 0.0 0.0 3.717230291709647E-4 9.293075729274118E-5 18-19 1.3939613593911177E-4 0.0 0.0 5.420960842076568E-4 9.293075729274118E-5 20-21 1.5488459548790195E-4 0.0 0.0 8.828421942810412E-4 9.293075729274118E-5 22-23 1.5488459548790195E-4 0.0 0.0 0.0013629844402935371 9.293075729274118E-5 24-25 2.1683843368306276E-4 0.0 0.0 0.0019515459031475648 9.293075729274118E-5 26-27 2.1683843368306276E-4 0.0 0.0 0.00312866882885562 1.2390767639032158E-4 28-29 3.0976919097580396E-4 0.0 0.0 0.00800753358672453 1.2390767639032158E-4 30-31 3.4074611007338433E-4 0.0 0.0 0.02676405810030946 1.3939613593911177E-4 32-33 3.562345696221745E-4 0.0 0.0 0.06897011037076274 2.0134997413427257E-4 34-35 4.801422460124961E-4 0.0 0.0 0.13670114397762229 2.1683843368306276E-4 36-37 6.195383819516078E-4 0.0 0.0 0.24400519173164076 2.1683843368306276E-4 38-39 6.814922201467687E-4 0.0 0.0 0.4146725274997599 2.1683843368306276E-4 40-41 6.814922201467687E-4 0.0 0.0 0.6060634221441604 2.1683843368306276E-4 42-43 7.744229774395098E-4 0.0 0.0 0.7900972985028856 2.1683843368306276E-4 44-45 8.67353734732251E-4 0.0 0.0 1.0078805282184244 2.1683843368306276E-4 46-47 9.293075729274118E-4 0.0 0.0 1.2555564848631284 2.1683843368306276E-4 48-49 9.293075729274118E-4 0.0 0.0 1.5089167061622386 2.4781535278064316E-4 50-51 9.293075729274118E-4 0.0 0.0 1.7701760418312316 2.4781535278064316E-4 52-53 9.293075729274118E-4 0.0 0.0 2.0158849641132393 2.4781535278064316E-4 54-55 9.602844920249922E-4 0.0 0.0 2.2753011730959263 2.4781535278064316E-4 56-57 9.912614111225726E-4 0.0 0.0 2.505506147369595 2.9428073142701374E-4 58-59 9.912614111225726E-4 0.0 0.0 2.669776749344064 3.097691909758039E-4 60-61 9.912614111225726E-4 6.195383819516079E-5 0.0 2.792755118161458 3.4074611007338433E-4 62-63 0.0010067498706713628 7.744229774395099E-5 0.0 2.8957843510800103 3.717230291709647E-4 64-65 0.001022238330220153 9.293075729274118E-5 0.0 3.0022055566397476 4.026999482685451E-4 66-67 0.001022238330220153 9.293075729274118E-5 3.0976919097580395E-5 3.092921464217012 4.181884078173353E-4 68-69 0.0010532152493177333 9.293075729274118E-5 3.0976919097580395E-5 3.198041639174651 4.336768673661255E-4 70-71 0.0010841921684153137 9.293075729274118E-5 3.0976919097580395E-5 3.391461522019943 4.646537864637059E-4 72-73 0.0011306575470616843 9.293075729274118E-5 3.0976919097580395E-5 3.628884118443348 4.646537864637059E-4 74-75 0.0011461460066104746 9.293075729274118E-5 3.0976919097580395E-5 3.8950223188702098 4.646537864637059E-4 76-77 0.0011461460066104746 9.293075729274118E-5 3.0976919097580395E-5 4.278222296566828 4.646537864637059E-4 78-79 0.0011461460066104746 9.293075729274118E-5 3.0976919097580395E-5 4.814432766145945 4.801422460124961E-4 80-81 0.0013939613593911175 9.293075729274118E-5 3.0976919097580395E-5 5.422370291895509 4.956307055612863E-4 82-83 0.0014559151975862784 9.293075729274118E-5 3.0976919097580395E-5 5.997131537291564 4.956307055612863E-4 84-85 0.0014714036571350686 9.293075729274118E-5 3.0976919097580395E-5 6.452616155702386 4.956307055612863E-4 86-87 0.0014868921166838588 9.293075729274118E-5 3.0976919097580395E-5 6.857515465226859 4.956307055612863E-4 88-89 0.0014868921166838588 9.293075729274118E-5 3.0976919097580395E-5 7.279250730280868 4.956307055612863E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5725 0.0 50.81875 1 GTATCAA 10765 0.0 41.831516 1 ATCAACG 13640 0.0 32.760715 3 TCAACGC 13715 0.0 32.54568 4 TATCAAC 13960 0.0 32.080765 2 CAACGCA 14015 0.0 31.916285 5 AACGCAG 14440 0.0 30.89009 6 GTACATG 14385 0.0 27.469353 1 TACATGG 14135 0.0 27.414639 2 ACGCAGA 16655 0.0 26.583956 7 CGCAGAG 16875 0.0 26.181557 8 ACATGGG 14535 0.0 25.684408 3 GCAGAGT 18980 0.0 22.955246 9 CATGGGA 8200 0.0 21.785353 4 CATGGGG 6455 0.0 21.102835 4 AGAGTAC 16810 0.0 20.762846 10-11 GAGTACT 10355 0.0 20.355387 12-13 AGTACTT 10805 0.0 19.878176 12-13 TGGTATC 3125 0.0 19.460152 2 GTACTTT 10680 0.0 19.405188 14-15 >>END_MODULE