FastQCFastQC Report
Fri 27 May 2016
SRR939832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481563
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100680.289180462912778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.21266310562238858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.14645720901790374No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36480.10478052529855125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.046.062341
GTATCAA111100.043.102261
TATCAAC144250.033.034382
ATCAACG144700.032.8685233
TCAACGC146200.032.531764
CAACGCA148700.031.9862065
AACGCAG153700.031.068556
GTACATG156050.027.7325881
TACATGG152800.027.6455732
ACGCAGA177900.026.3926727
CGCAGAG178350.026.1154278
ACATGGG160550.025.4335523
GCAGAGT193950.022.6807219
CATGGGA89600.021.4743044
TACCTGG24150.021.0367772
AGAGTAC167000.020.45114910-11
AGTACTT105050.020.42064512-13
GAGTACT97950.020.24388312-13
GAGTACA114850.020.0692631
CATGGGG76300.019.9150684
Produced by FastQC (version 0.11.5)