##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939832_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3481563 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.966795373227484 31.0 30.0 33.0 26.0 34.0 2 30.020315300915136 31.0 30.0 33.0 25.0 34.0 3 29.969076245353023 31.0 30.0 34.0 25.0 34.0 4 33.61067629682416 35.0 33.0 37.0 28.0 37.0 5 33.47769292125405 35.0 33.0 37.0 28.0 37.0 6 33.45235918465356 35.0 33.0 37.0 28.0 37.0 7 33.434342851184944 35.0 33.0 37.0 28.0 37.0 8 33.27178913608629 35.0 33.0 37.0 26.0 37.0 9 34.563840723261364 37.0 34.0 39.0 27.0 39.0 10-11 34.35527333556796 37.0 33.0 39.0 27.0 39.0 12-13 34.24749501876025 37.0 33.0 39.0 27.0 39.0 14-15 34.83483797363426 38.0 33.0 40.0 25.0 41.0 16-17 34.62112261073547 38.0 33.0 40.0 25.0 41.0 18-19 34.649446670934864 38.0 33.0 40.0 25.0 41.0 20-21 34.768836008424955 38.0 33.0 40.0 25.0 41.0 22-23 34.678300091079784 38.0 33.0 40.0 25.0 41.0 24-25 34.50453187261009 38.0 33.0 40.0 24.0 41.0 26-27 34.39249325662066 38.0 33.0 40.0 24.0 41.0 28-29 34.26927632790215 38.0 33.0 40.0 23.5 41.0 30-31 34.085566884758364 38.0 33.0 40.0 23.0 41.0 32-33 33.807785181540595 38.0 32.0 40.0 21.0 41.0 34-35 33.53539358615656 38.0 32.0 40.0 19.0 41.0 36-37 33.320794568416545 37.0 31.0 40.0 18.0 41.0 38-39 33.041037746552334 37.0 31.0 40.0 16.5 41.0 40-41 32.74509336754785 37.0 30.5 40.0 15.5 41.0 42-43 32.427530968131265 37.0 30.0 40.0 15.0 41.0 44-45 32.02493348533403 37.0 30.0 39.0 10.5 41.0 46-47 31.63778280042613 36.0 30.0 39.0 9.0 40.5 48-49 31.291151129535784 36.0 29.5 39.0 8.0 40.0 50-51 30.555054152402242 35.5 28.0 38.5 7.0 40.0 52-53 30.67152827623685 35.5 28.0 38.5 4.0 40.0 54-55 30.749614325519886 36.0 28.0 39.0 2.0 40.0 56-57 30.516452237113043 35.0 28.0 39.0 2.0 40.0 58-59 29.976499060910285 35.0 27.5 38.5 2.0 40.0 60-61 29.486834361463515 34.0 26.5 38.0 2.0 40.0 62-63 29.040271136842847 34.0 26.0 38.0 2.0 40.0 64-65 28.507891570538863 34.0 26.0 38.0 2.0 40.0 66-67 27.969322973618457 33.5 25.0 37.0 2.0 39.0 68-69 27.425721148805867 33.0 23.5 36.5 2.0 39.0 70-71 26.877241916920646 32.0 22.5 36.0 2.0 39.0 72-73 26.400402204412217 32.0 21.5 35.5 2.0 38.0 74-75 25.810268405311064 31.5 20.0 35.0 2.0 38.0 76-77 25.16353114965893 31.0 18.0 35.0 2.0 37.0 78-79 24.510422474043985 31.0 14.5 34.0 2.0 36.0 80-81 23.833641671858302 30.0 7.5 34.0 2.0 36.0 82-83 23.086278059595646 29.5 4.5 34.0 2.0 35.0 84-85 22.342048384590484 29.0 2.0 34.0 2.0 35.0 86-87 21.548671674187716 29.0 2.0 34.0 2.0 35.0 88-89 20.687276231968227 27.5 2.0 33.0 2.0 35.0 90-91 19.734924371611257 26.0 2.0 32.5 2.0 34.0 92-93 18.754796193548703 25.0 2.0 32.0 2.0 34.0 94-95 17.38110670408664 21.5 2.0 32.0 2.0 34.0 96-97 14.817070666249613 2.0 2.0 31.0 2.0 34.0 98-99 13.436583798713393 2.0 2.0 30.0 2.0 34.0 100-101 12.167100092688255 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5709607617644892 1101 2 0.5805785210061813 1101 3 0.7115672487930489 1101 4 0.8316539484974825 1101 5 0.7654398639809941 1101 6 0.8478371218163119 1101 7 0.7508655124455998 1101 8 0.6975906921998245 1101 9 0.6813734903870667 1101 10-11 0.8597862414636737 1101 12-13 0.5102904907366295 1101 14-15 0.8711685180663054 1101 16-17 0.5964032288081498 1101 18-19 0.6489217571600179 1101 20-21 0.7516288529476043 1101 22-23 0.6430376143660936 1101 24-25 0.6300583064318772 1101 26-27 0.7380729478577166 1101 28-29 0.9198384528298789 1101 30-31 0.8879669196185418 1101 32-33 0.9843668209173515 1101 34-35 0.7272031665061505 1101 36-37 0.9001567453344137 1101 38-39 0.7025931109179453 1101 40-41 0.7293983965911579 1101 42-43 0.6209548292359273 1101 44-45 0.6548276958338803 1101 46-47 0.6278169887248524 1101 48-49 0.4261022776617125 1101 50-51 0.39485742166919024 1101 52-53 0.6544429427981626 1101 54-55 0.5856303995890926 1101 56-57 0.3408719648015399 1101 58-59 0.1823631554749987 1101 60-61 0.29074050130230233 1101 62-63 0.04015231501459837 1101 64-65 0.29474620821041597 1101 66-67 -0.08047031001861882 1101 68-69 -9.455585007600575E-5 1101 70-71 0.04088696050179763 1101 72-73 -0.1588822748683647 1101 74-75 -0.16194507774651967 1101 76-77 -0.6285354971278672 1101 78-79 -0.5296870559915945 1101 80-81 -0.7610529187925508 1101 82-83 -0.7877215138411167 1101 84-85 -0.6807412841959035 1101 86-87 -0.7655127934415482 1101 88-89 -0.7641376706261482 1101 90-91 -0.9697197881638004 1101 92-93 -0.8868530440482303 1101 94-95 -0.5953576755464489 1101 96-97 0.873936029288771 1101 98-99 0.7754896037723427 1101 100-101 0.13670487223956052 1102 1 0.8362104928434668 1102 2 0.7420393567232537 1102 3 0.8731310708971556 1102 4 0.9420108443306816 1102 5 1.0092138786827363 1102 6 0.8632994805100438 1102 7 0.8755139513945238 1102 8 0.8250917667929514 1102 9 1.047324270748959 1102 10-11 0.8981824903154418 1102 12-13 0.7943625113862751 1102 14-15 0.9427320565214643 1102 16-17 0.9868030210898411 1102 18-19 0.9946073475752968 1102 20-21 0.9461609740089756 1102 22-23 0.7852026849619591 1102 24-25 0.8758789573844012 1102 26-27 1.156655663163633 1102 28-29 1.2403312165088494 1102 30-31 0.9897021966017405 1102 32-33 1.0019668017164989 1102 34-35 1.0744998829422983 1102 36-37 0.8204945930498369 1102 38-39 0.9631364513861413 1102 40-41 0.9033454589699161 1102 42-43 0.7296401600033278 1102 44-45 0.5999776261217065 1102 46-47 0.6278407715981302 1102 48-49 0.6374006698085637 1102 50-51 0.7861945646260793 1102 52-53 0.6737319439829434 1102 54-55 0.5096572109603628 1102 56-57 0.5025311365902532 1102 58-59 0.4991407725183734 1102 60-61 0.6533786983423155 1102 62-63 0.48082342842399584 1102 64-65 0.3029761733215963 1102 66-67 0.2230552282052578 1102 68-69 -0.11709843747682314 1102 70-71 0.2712589988249512 1102 72-73 0.21050676711822192 1102 74-75 0.10758906158620718 1102 76-77 -0.060204465714836175 1102 78-79 -0.22333877435873717 1102 80-81 -0.3939413596615644 1102 82-83 -0.5505584833357631 1102 84-85 -0.7479946678005582 1102 86-87 -0.9282624863370152 1102 88-89 -0.6650342194912255 1102 90-91 -0.7345658649715716 1102 92-93 -0.7434981752140466 1102 94-95 -0.5340489193034443 1102 96-97 0.6725537861492565 1102 98-99 0.7255534247854953 1102 100-101 0.25962872558869066 1103 1 0.685459726798971 1103 2 0.7665506585320543 1103 3 0.8326066805497412 1103 4 0.8604667872232312 1103 5 0.9450265648888418 1103 6 0.905833411400522 1103 7 0.747113010921062 1103 8 0.6908595513328564 1103 9 0.9638585241844879 1103 10-11 0.757963456762262 1103 12-13 0.7061553525837567 1103 14-15 0.8986361163450809 1103 16-17 0.876512136382722 1103 18-19 0.9330666007986324 1103 20-21 0.825048490666731 1103 22-23 0.9381195956594155 1103 24-25 1.0090094268572045 1103 26-27 1.1752011547477466 1103 28-29 1.2960046141814345 1103 30-31 1.176939598246726 1103 32-33 1.2145738569650746 1103 34-35 1.191414187815269 1103 36-37 0.9140027373123587 1103 38-39 0.9326455463077536 1103 40-41 1.002104509329392 1103 42-43 0.8747444778629898 1103 44-45 0.9266837196767383 1103 46-47 0.6815033555235033 1103 48-49 0.64149062592546 1103 50-51 0.834960617608349 1103 52-53 0.9582688796352059 1103 54-55 0.9540341048515302 1103 56-57 0.6937997147607433 1103 58-59 0.6344017369280728 1103 60-61 0.9334384116167449 1103 62-63 0.7133357572841383 1103 64-65 0.7345560923458123 1103 66-67 0.5720977786878017 1103 68-69 0.41639510737815755 1103 70-71 0.43851770541929724 1103 72-73 0.397065126552242 1103 74-75 0.3945762786165865 1103 76-77 0.10114933225312939 1103 78-79 -0.01956414846855381 1103 80-81 -0.3174733552817415 1103 82-83 -0.0859959987203105 1103 84-85 -0.3189081068947175 1103 86-87 -0.3271671063704602 1103 88-89 -0.3971760459450131 1103 90-91 -0.569077336540925 1103 92-93 -0.6232207015905935 1103 94-95 -0.37630529169019056 1103 96-97 1.1518650675867868 1103 98-99 1.3485162981526102 1103 100-101 0.9170718770235915 1104 1 0.4554210210303147 1104 2 0.43263651915506784 1104 3 0.6432834470457891 1104 4 0.7964625758512085 1104 5 0.7736097296081468 1104 6 0.7629382495392818 1104 7 0.63903468401125 1104 8 0.6711495729494814 1104 9 0.7462347225239796 1104 10-11 0.7781921599461512 1104 12-13 0.766098833271208 1104 14-15 0.6799935100359278 1104 16-17 0.7588815023555568 1104 18-19 0.9352837449352194 1104 20-21 0.8387105486414157 1104 22-23 0.8192375684056827 1104 24-25 0.9008701848712377 1104 26-27 1.0039731562031236 1104 28-29 1.2472278547647022 1104 30-31 1.1620032215742313 1104 32-33 1.2004767501019202 1104 34-35 1.0969953670323633 1104 36-37 0.8378921073023022 1104 38-39 0.6790094513263298 1104 40-41 0.9785318565766019 1104 42-43 0.7596029243968374 1104 44-45 0.8330204104734591 1104 46-47 0.7504232489056548 1104 48-49 0.7295398288434605 1104 50-51 0.8966548716703109 1104 52-53 0.8934143267271679 1104 54-55 0.9094302339453932 1104 56-57 0.8060430276847832 1104 58-59 0.6191522238370943 1104 60-61 0.8309592914938584 1104 62-63 0.48009699323457156 1104 64-65 0.500843919373338 1104 66-67 0.5746895505427325 1104 68-69 0.320226295238605 1104 70-71 0.4713330685307753 1104 72-73 0.635139189406722 1104 74-75 0.5564792079732079 1104 76-77 0.35801680738907393 1104 78-79 0.4172576979826488 1104 80-81 0.49464467014783153 1104 82-83 0.44654121298262694 1104 84-85 0.3593991701519883 1104 86-87 0.21784839404689293 1104 88-89 0.2471361734546278 1104 90-91 -0.012662896704441096 1104 92-93 0.19494705523642253 1104 94-95 0.678584377936069 1104 96-97 2.1446270394571947 1104 98-99 2.0787880040875493 1104 100-101 2.0046708022840853 1105 1 0.7225866634753864 1105 2 0.8303063876461998 1105 3 0.8174450617924016 1105 4 0.8479287693259536 1105 5 0.754744508685782 1105 6 0.8854406926207332 1105 7 0.8108604452212589 1105 8 0.9267386877614072 1105 9 0.9878014233358172 1105 10-11 0.9943122260395754 1105 12-13 0.7756649176427501 1105 14-15 0.913879951346594 1105 16-17 0.9727632057631439 1105 18-19 0.8855792788525036 1105 20-21 0.8656072846333132 1105 22-23 0.7745170766382685 1105 24-25 0.9381523611395082 1105 26-27 1.1131525308736485 1105 28-29 1.032313942431621 1105 30-31 1.1854221026950214 1105 32-33 1.1646768915658328 1105 34-35 1.2856166821146147 1105 36-37 1.1691551992456723 1105 38-39 1.149324709409818 1105 40-41 0.9979954861982421 1105 42-43 1.1616864076595874 1105 44-45 1.1057000360637161 1105 46-47 0.9905829239329478 1105 48-49 1.122557143827617 1105 50-51 1.050728001749313 1105 52-53 1.017916073548438 1105 54-55 1.0227951036121858 1105 56-57 0.9268627618525151 1105 58-59 1.0081239289709067 1105 60-61 1.1924639010920863 1105 62-63 1.1190717837754995 1105 64-65 1.0241416281419973 1105 66-67 1.0059523299383706 1105 68-69 0.9733208704197196 1105 70-71 0.9543666552307961 1105 72-73 1.0576024678529343 1105 74-75 1.0490168503407027 1105 76-77 0.928984857477495 1105 78-79 0.7103235299919284 1105 80-81 0.6507704374324845 1105 82-83 0.7499021669873009 1105 84-85 0.661555573205213 1105 86-87 0.6582685014767584 1105 88-89 0.7974234609201645 1105 90-91 0.8723428982511052 1105 92-93 0.8671446280388047 1105 94-95 0.872888972852568 1105 96-97 2.2466144503550467 1105 98-99 2.4028378149000016 1105 100-101 1.8938730714768006 1106 1 0.9758535812890479 1106 2 0.9918171687002477 1106 3 0.9084285078797585 1106 4 0.9143582143042011 1106 5 0.9618265918133062 1106 6 1.0575565773814546 1106 7 1.0716075460953576 1106 8 1.0728445577694856 1106 9 1.4096440034821 1106 10-11 1.297826604737459 1106 12-13 1.1321505061794355 1106 14-15 1.4968029386688286 1106 16-17 1.4337888946767166 1106 18-19 1.3552111347108422 1106 20-21 1.2871218009459824 1106 22-23 1.1519899038604393 1106 24-25 1.242222441472137 1106 26-27 1.5874484223348944 1106 28-29 1.4346088329090847 1106 30-31 1.3560790249238224 1106 32-33 1.535530979749062 1106 34-35 1.5079031109206724 1106 36-37 1.483726803580204 1106 38-39 1.443062581082387 1106 40-41 1.5224863319476256 1106 42-43 1.4621495060074707 1106 44-45 1.6184484082363682 1106 46-47 1.5270212729158672 1106 48-49 1.425104983398267 1106 50-51 1.4667657770096163 1106 52-53 1.7184715703992737 1106 54-55 1.5874993260108 1106 56-57 1.4615158046548125 1106 58-59 1.5084835704843762 1106 60-61 1.7440506487876526 1106 62-63 1.6548463461727465 1106 64-65 1.651490110570311 1106 66-67 1.6394009566164698 1106 68-69 1.5646225097559245 1106 70-71 1.5773802307661633 1106 72-73 1.6797922386505846 1106 74-75 1.7257802651206937 1106 76-77 1.7155452986311985 1106 78-79 1.3858013932601665 1106 80-81 1.361807635202144 1106 82-83 1.3819495801978299 1106 84-85 1.4658027916252792 1106 86-87 1.519447983111327 1106 88-89 1.510689895443786 1106 90-91 1.4647339061074334 1106 92-93 1.6648109514876275 1106 94-95 1.8366870264224033 1106 96-97 3.411603731964874 1106 98-99 3.4376272437904234 1106 100-101 2.895357713911796 1107 1 0.5797409631545527 1107 2 0.5949998051651804 1107 3 0.6434925659463815 1107 4 0.22274073786545756 1107 5 0.5437603626483849 1107 6 0.659701747221078 1107 7 0.4696811271275365 1107 8 0.47893815622953184 1107 9 0.6942076258521226 1107 10-11 0.6568323139218748 1107 12-13 0.6466140332049335 1107 14-15 0.6417774122636501 1107 16-17 0.7591601803837378 1107 18-19 0.6357796818473034 1107 20-21 0.6874157935842717 1107 22-23 0.5821983243741187 1107 24-25 0.6402650068143245 1107 26-27 0.8269023492658647 1107 28-29 0.8330983770112681 1107 30-31 0.9513672657106014 1107 32-33 0.7745465844155319 1107 34-35 0.812651260235782 1107 36-37 0.6739047581528865 1107 38-39 0.6862491396372192 1107 40-41 0.6748326114597134 1107 42-43 0.6006543774400512 1107 44-45 0.5593767291716247 1107 46-47 0.4814903220470157 1107 48-49 0.6047888449008987 1107 50-51 0.7280489650286981 1107 52-53 0.7311429567021506 1107 54-55 0.6685395495863311 1107 56-57 0.4834082868782268 1107 58-59 0.4386017747251536 1107 60-61 0.578580834931028 1107 62-63 0.4931630311370263 1107 64-65 0.462303386270257 1107 66-67 0.6647087750319116 1107 68-69 0.3764494569750987 1107 70-71 0.46067819964872925 1107 72-73 0.20153805276321535 1107 74-75 0.30509103577033514 1107 76-77 0.30296880557034456 1107 78-79 0.18823296591975236 1107 80-81 0.21665789834319682 1107 82-83 0.2669173091108519 1107 84-85 0.06710192236543833 1107 86-87 0.03415409714075679 1107 88-89 0.14983391163102056 1107 90-91 0.10459276010230312 1107 92-93 0.3370588013126117 1107 94-95 0.8606697035851703 1107 96-97 2.0790882528381047 1107 98-99 2.2763634610906163 1107 100-101 1.852599015980001 1108 1 0.5526878698625559 1108 2 0.5120415364222275 1108 3 0.5746764446909474 1108 4 0.5543561348698063 1108 5 0.6802073297525908 1108 6 0.6199855841916033 1108 7 0.6449902172588011 1108 8 0.5508964526540794 1108 9 0.5071524186931597 1108 10-11 0.701174291041788 1108 12-13 0.44080517710894895 1108 14-15 0.6591642826332773 1108 16-17 0.6123607700016294 1108 18-19 0.5850616591181961 1108 20-21 0.5949926225825024 1108 22-23 0.5704809899100383 1108 24-25 0.537349254479011 1108 26-27 0.6829288204166204 1108 28-29 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0.03263550522577319 2312 2 -0.25353124662530035 2312 3 -0.6191228185753239 2312 4 -0.2642368978064056 2312 5 -1.8475963513843254 2312 6 -0.5006271369129109 2312 7 -0.08628921411440871 2312 8 0.21356228103647368 2312 9 0.2795445247558135 2312 10-11 0.09958410023366326 2312 12-13 0.12074585021183992 2312 14-15 0.3961263952784222 2312 16-17 -0.33126206325856344 2312 18-19 -0.07492317159338313 2312 20-21 0.34203577171833643 2312 22-23 0.2482182658518397 2312 24-25 0.327307354330479 2312 26-27 0.6530855134695486 2312 28-29 0.5157601162817471 2312 30-31 0.32798928612672285 2312 32-33 -0.2879215810409832 2312 34-35 0.14758068459416762 2312 36-37 0.5564599154478813 2312 38-39 0.5791035424021587 2312 40-41 0.6137889894962214 2312 42-43 0.645785104194708 2312 44-45 0.755439384120038 2312 46-47 0.06690942371692188 2312 48-49 0.44323167637822536 2312 50-51 0.5856284482533951 2312 52-53 0.4399919474805216 2312 54-55 0.4125424772226296 2312 56-57 0.3057040213766875 2312 58-59 0.5378532424251006 2312 60-61 0.5866267731857882 2312 62-63 0.7005558912483174 2312 64-65 0.6034201519506617 2312 66-67 0.7914680283994109 2312 68-69 0.6762314141377139 2312 70-71 0.7206322696142387 2312 72-73 0.8056070211885249 2312 74-75 0.9065814252013702 2312 76-77 0.7173158446419272 2312 78-79 0.8663106070413491 2312 80-81 0.8753839046496701 2312 82-83 1.1391996323802331 2312 84-85 1.009251649528366 2312 86-87 0.985067857000903 2312 88-89 0.9455920814442784 2312 90-91 1.1559033462049833 2312 92-93 1.1576492850941555 2312 94-95 1.6582315390937836 2312 96-97 3.4486605107441086 2312 98-99 3.8639918382162044 2312 100-101 3.5594420689785586 2313 1 -0.025944964065036658 2313 2 -0.6731753536627956 2313 3 -1.0558006008481975 2313 4 -0.1700189141075512 2313 5 -0.1418270303093152 2313 6 0.10571878740843488 2313 7 0.10709322520292375 2313 8 0.24531878926563166 2313 9 0.4296423905801561 2313 10-11 -0.701663135369742 2313 12-13 -0.017520605549158574 2313 14-15 0.021880609478742485 2313 16-17 -0.5364960220552248 2313 18-19 -0.10776569938622771 2313 20-21 -2.296051439893006 2313 22-23 -0.39640458001493073 2313 24-25 0.14171785069011378 2313 26-27 0.09334266068187702 2313 28-29 -0.02907077130713276 2313 30-31 0.26616097950546447 2313 32-33 0.36429173608828336 2313 34-35 0.24765184155886288 2313 36-37 -0.611212876282714 2313 38-39 0.1925353138572703 2313 40-41 0.30182310187993977 2313 42-43 0.21008790592890136 2313 44-45 0.28071688295013786 2313 46-47 0.21553130285036914 2313 48-49 0.046805501043969855 2313 50-51 0.30848081077276035 2313 52-53 0.2821021088609932 2313 54-55 0.22135429738178658 2313 56-57 0.43475432809897896 2313 58-59 0.4553329022878465 2313 60-61 0.3274179568623019 2313 62-63 0.2938126596777657 2313 64-65 0.16141496690626056 2313 66-67 0.31054897550179206 2313 68-69 0.07870482045353455 2313 70-71 0.3218840677959953 2313 72-73 0.28877462528233977 2313 74-75 0.3519647699238675 2313 76-77 0.42211228476423557 2313 78-79 0.3057117893713155 2313 80-81 0.3297464183873977 2313 82-83 0.5035584738349321 2313 84-85 0.4906460327277671 2313 86-87 0.4579086279048106 2313 88-89 0.7109629709204093 2313 90-91 0.8672656032236148 2313 92-93 0.894411734303997 2313 94-95 1.2588628710600211 2313 96-97 2.7085010515933305 2313 98-99 2.9813372056955956 2313 100-101 2.5906417722924573 2314 1 -0.07276495111579351 2314 2 -0.14016786598254072 2314 3 -1.0620457461979456 2314 4 -0.03638301088441409 2314 5 -0.4016309246125971 2314 6 -0.27896193200657393 2314 7 -0.0618946038027417 2314 8 -1.4799641916516002 2314 9 -1.4451151117946424 2314 10-11 -0.6593152893349341 2314 12-13 -0.3003882883218907 2314 14-15 -0.44156529286197355 2314 16-17 -0.506156327698811 2314 18-19 -1.593412583458047 2314 20-21 -1.0511633386886885 2314 22-23 -1.588323946557395 2314 24-25 -1.4759605625452252 2314 26-27 -1.60599489706469 2314 28-29 -2.821659818389371 2314 30-31 -1.4011530615523853 2314 32-33 -1.2106624101569636 2314 34-35 -1.5796557624601242 2314 36-37 -3.1280899969390106 2314 38-39 -3.4605569226559503 2314 40-41 -4.098045504056181 2314 42-43 -2.823722558002409 2314 44-45 -3.1886850091063437 2314 46-47 -3.1223817060263386 2314 48-49 -1.3835868757101117 2314 50-51 -1.042873162174537 2314 52-53 -2.304718129237287 2314 54-55 -1.7342215657970144 2314 56-57 -1.6671463121022647 2314 58-59 -3.7495888092188636 2314 60-61 -2.137607064457942 2314 62-63 -2.039953329756891 2314 64-65 -1.3911914400369625 2314 66-67 -2.4672799429779637 2314 68-69 -2.1943744517852544 2314 70-71 -1.7707575414216272 2314 72-73 -1.3801854306516468 2314 74-75 -1.7032378742830154 2314 76-77 -0.5445023784855785 2314 78-79 -0.2753645340362212 2314 80-81 -0.1422791457667394 2314 82-83 -0.23601286928584742 2314 84-85 0.0158785636160097 2314 86-87 0.3018467021478308 2314 88-89 0.44920173274801556 2314 90-91 0.8584286552820011 2314 92-93 0.9490953340007522 2314 94-95 1.5870401968988084 2314 96-97 3.1189712666087743 2314 98-99 3.2647381389610004 2314 100-101 2.9840728132211716 2315 1 -2.595003274678387 2315 2 -1.9605517095455127 2315 3 -1.583878702838497 2315 4 -1.6573004880771407 2315 5 -2.0316842313073167 2315 6 -3.170607013734667 2315 7 -2.36041020529132 2315 8 -1.314685406123342 2315 9 -1.0798868441273513 2315 10-11 -3.4400160912965276 2315 12-13 -2.0007483130836548 2315 14-15 -1.334872643422301 2315 16-17 -2.3105959530748024 2315 18-19 -1.4588010711734611 2315 20-21 -1.075514443715896 2315 22-23 -1.1934396317789862 2315 24-25 -3.0804802866116248 2315 26-27 -4.2337162294819635 2315 28-29 -5.966163756946475 2315 30-31 -8.743346438876905 2315 32-33 -7.192583007654964 2315 34-35 -6.629559490649552 2315 36-37 -4.11101917605097 2315 38-39 -6.603435842314191 2315 40-41 -4.3680794513636165 2315 42-43 -4.302216728312281 2315 44-45 -3.3712358256090162 2315 46-47 -2.4174668757700566 2315 48-49 -3.1815251787890197 2315 50-51 -3.092702692727908 2315 52-53 -2.413023897242134 2315 54-55 -3.71596486721252 2315 56-57 -3.6275677667642974 2315 58-59 -2.493391738786343 2315 60-61 -2.729364530670633 2315 62-63 -3.3350054807969833 2315 64-65 -2.6366500271401883 2315 66-67 -3.092938135322566 2315 68-69 -2.2687486283482734 2315 70-71 -2.7329881490345294 2315 72-73 -1.9094911923852074 2315 74-75 -2.5099730551374826 2315 76-77 -1.7271514577435418 2315 78-79 -1.545441360323764 2315 80-81 -1.262018641039969 2315 82-83 -1.2924877728421436 2315 84-85 -1.1946675045401847 2315 86-87 -1.669521812803108 2315 88-89 -2.179748507461163 2315 90-91 -1.9857649603514247 2315 92-93 -1.128496881684974 2315 94-95 -0.5976174320122141 2315 96-97 0.8138856231184377 2315 98-99 0.8575921359204095 2315 100-101 0.5214415610408594 2316 1 -3.6782882411721154 2316 2 -2.332167453327358 2316 3 -2.479258459536979 2316 4 -3.0647766585888014 2316 5 -2.6726201945221177 2316 6 -6.197033061633544 2316 7 -3.9125693085981865 2316 8 -4.785162666576991 2316 9 -6.8356409047795985 2316 10-11 -5.875055859839769 2316 12-13 -3.981819808149325 2316 14-15 -5.291284940723514 2316 16-17 -4.672665031088272 2316 18-19 -3.028100889283799 2316 20-21 -3.705624011488574 2316 22-23 -4.568871538172949 2316 24-25 -3.2322133216865723 2316 26-27 -4.073819971990414 2316 28-29 -4.062606893703315 2316 30-31 -3.6970707917437693 2316 32-33 -3.5717207243972027 2316 34-35 -5.715369130301042 2316 36-37 -6.000635426337944 2316 38-39 -5.85808816326243 2316 40-41 -5.223635849071201 2316 42-43 -4.555322789849463 2316 44-45 -3.838878599588007 2316 46-47 -4.117523656543014 2316 48-49 -4.7766652939195495 2316 50-51 -5.592217431071273 2316 52-53 -3.4837954708631393 2316 54-55 -3.854984365535728 2316 56-57 -3.142201713624356 2316 58-59 -3.6602319570539414 2316 60-61 -3.7805883141365015 2316 62-63 -2.8341046816022946 2316 64-65 -3.276300191117052 2316 66-67 -4.006542137835972 2316 68-69 -3.30837138824284 2316 70-71 -2.8356016426771937 2316 72-73 -2.784246569981139 2316 74-75 -3.7822570806970255 2316 76-77 -3.9778030633936368 2316 78-79 -3.903316824702326 2316 80-81 -4.251517991520732 2316 82-83 -3.256957947686562 2316 84-85 -3.0976297022322647 2316 86-87 -2.7204403351252218 2316 88-89 -2.806327333877544 2316 90-91 -2.1894256207291143 2316 92-93 -1.859622889023985 2316 94-95 -1.8524679621658855 2316 96-97 -0.2892213640272878 2316 98-99 -0.019356251085319798 2316 100-101 -0.30163106023244524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30979.0 3 10334.0 4 10077.0 5 11391.0 6 12705.0 7 14828.0 8 18289.0 9 21974.0 10 26093.0 11 30703.0 12 34817.0 13 38706.0 14 41954.0 15 46479.0 16 50596.0 17 54372.0 18 51544.0 19 51677.0 20 55161.0 21 61307.0 22 67743.0 23 75686.0 24 83658.0 25 94326.0 26 104652.0 27 118433.0 28 132777.0 29 149631.0 30 169074.0 31 191065.0 32 217385.0 33 247537.0 34 281534.0 35 306924.0 36 299893.0 37 216973.0 38 49895.0 39 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76522346304451 18.606323822407504 13.219473701162753 23.40897901338524 2 15.909937920348943 19.84504242742133 40.23772468934293 24.007294962886792 3 19.79787974768186 24.425696691926664 29.0339280059844 26.74249555440707 4 12.83276332707237 15.832070115084695 36.84404456116229 34.49112199668063 5 13.798279799130608 37.56403725730847 34.02552487468674 14.612158068874189 6 32.64992016779289 35.59265238804867 17.462644538576747 14.294782905581696 7 29.243945152267237 32.39241419542353 20.321609670727202 18.042030981582034 8 26.87657363346123 35.00977583606627 19.402007059542996 18.71164347092951 9 27.179649014601186 16.189722210395622 19.746812283453334 36.88381649154986 10-11 25.471980480595157 26.448813700518837 27.15611022008664 20.923095598799367 12-13 26.175198124469627 23.719360823852604 27.603276424985978 22.50216462669179 14-15 23.149808903420872 25.064873319420062 25.771591267312015 26.01372650984705 16-17 22.53983402537366 28.329473324520315 26.42186099596281 22.70883165414321 18-19 22.509098713162473 26.863751031705323 28.71178734658193 21.91536290855027 20-21 23.384763033925474 26.141261651179722 28.687269242612167 21.786706072282637 22-23 22.909898065148965 26.0646803915293 28.622002915055827 22.40341862826591 24-25 22.523414675782902 26.300435885983635 28.911721537327534 22.26442790090593 26-27 22.429382841153135 26.516570418521102 28.72933735598763 22.324709384338135 28-29 22.2566344102784 26.6979802223962 28.817093510706766 22.228291856618636 30-31 22.515722473777643 26.50302905291195 28.686520840491468 22.294727632818937 32-33 22.26890060766669 26.56919144991446 28.625383525272902 22.536524417145944 34-35 22.629112602329386 26.663647582833416 28.563641366226094 22.143598448611108 36-37 22.688452807318875 26.330349271495525 28.59569811061216 22.385499810573442 38-39 22.359458753154414 26.62734393707749 28.61357445799333 22.39962285177476 40-41 22.612374080926923 26.498039296376973 28.656503977595904 22.233082645100197 42-43 22.363999574653555 26.460678692344587 28.568560998004354 22.606760734997508 44-45 22.419044179174495 26.629041855204434 28.477857170095184 22.47405679552589 46-47 22.53799333038761 26.780782189803293 28.091791368076535 22.58943311173256 48-49 22.425304096184494 26.802887054965264 28.094299867290136 22.67750898156011 50-51 22.357835128313074 26.996884964844277 27.97676191836408 22.668517988478573 52-53 22.51138780278192 27.016870882937766 27.82815822882514 22.643583085455177 54-55 22.277696701170026 27.007222017830774 27.955163155973555 22.75991812502565 56-57 22.243200824096295 27.20952973623247 27.79855244768029 22.748716991990943 58-59 22.253509165249422 26.87381111575729 27.945500070892226 22.92717964810106 60-61 22.163157566011684 26.957756378392833 28.02968442703046 22.849401628565023 62-63 22.081710704465195 26.881814195845926 28.119321878195887 22.917153221492995 64-65 21.972958479442887 26.903217980946415 28.032171032459235 23.091652507151462 66-67 22.064880839333092 26.984452424938276 27.75121290077522 23.199453834953413 68-69 22.189104034802444 26.762996706263166 27.882832697133193 23.1650665618012 70-71 22.06723730190307 26.67732440807446 27.88304016150651 23.372398128515957 72-73 22.14309825256638 26.739253884205443 27.788246657391742 23.32940120583644 74-75 21.997725100525596 27.002158433604595 27.622537139151955 23.37757932671786 76-77 22.07549318747654 26.846513004823418 27.618743463895363 23.459250343804683 78-79 22.156910312180788 26.92703448047493 27.566095555381192 23.34995965196309 80-81 22.168473475934366 26.884386043595228 27.491125529750622 23.456014950719783 82-83 22.18281981993785 26.87489390937979 27.549324309829675 23.392961960852688 84-85 22.234199156259546 26.78752141668704 27.362083157350135 23.61619626970328 86-87 22.151340195679495 26.638743434396357 27.553217536219766 23.656698833704386 88-89 22.25818380249161 26.654699305291363 27.37541831426602 23.711698577951008 90-91 22.15640826920995 26.751455011527238 27.4469182775243 23.645218441738507 92-93 22.378859597437405 26.471051012826948 27.41710377832324 23.732985611412406 94-95 22.291875114271274 26.35804664139622 27.459499380235787 23.89057886409672 96-97 22.400871045481217 26.150723101214023 27.402614899183853 24.045790954120907 98-99 22.438553739252388 26.124501107226134 27.13979570802765 24.297149445493833 100-101 22.457001352623987 26.142581176527873 27.362564744501878 24.03785272634626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4588.0 1 2654.0 2 699.5 3 714.5 4 681.5 5 656.5 6 935.5 7 1619.5 8 2423.0 9 2777.0 10 2617.0 11 2418.5 12 2472.5 13 2684.5 14 2945.0 15 3223.5 16 3549.0 17 3918.0 18 4307.0 19 4815.5 20 5482.0 21 6367.0 22 7479.5 23 9055.0 24 11170.0 25 13831.5 26 17695.5 27 22468.5 28 26807.5 29 31167.0 30 36193.5 31 42323.5 32 49994.5 33 57159.5 34 63600.0 35 70914.5 36 80268.0 37 88644.5 38 93427.0 39 97465.5 40 102417.5 41 107777.0 42 112172.0 43 116414.0 44 120969.5 45 125508.5 46 128498.0 47 131248.5 48 134691.0 49 135171.0 50 132670.0 51 129738.5 52 127543.5 53 124906.0 54 121384.0 55 113948.0 56 104150.0 57 94834.5 58 85390.0 59 75077.0 60 63202.0 61 52050.0 62 41657.0 63 31822.0 64 23605.0 65 17340.5 66 12682.0 67 9332.0 68 6873.0 69 5080.0 70 3728.5 71 2672.0 72 1933.5 73 1461.0 74 1129.5 75 798.0 76 553.5 77 376.0 78 250.5 79 175.5 80 120.5 81 91.0 82 55.5 83 31.0 84 27.0 85 24.0 86 15.5 87 10.0 88 8.5 89 7.5 90 5.0 91 4.5 92 3.5 93 2.0 94 2.0 95 2.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12347902364541444 2 0.11761958637542964 3 0.12994163828142705 4 0.14341259945604892 5 0.14005203984532236 6 0.14062649447963457 7 0.14864013662829023 8 0.14746250462795016 9 0.15530381038631214 10-11 0.15014808004335983 12-13 0.14973160043348346 14-15 0.13749571672263292 16-17 0.14036798989419408 18-19 0.15860692453360747 20-21 0.18936897020102753 22-23 0.20643027284010085 24-25 0.22499951889424374 26-27 0.22699574874847878 28-29 0.22942281957844796 30-31 0.22841752396840156 32-33 0.23065789704221923 34-35 0.20103039927756589 36-37 0.18961311342061024 38-39 0.20298354503422744 40-41 0.19136520005526253 42-43 0.19383535498280513 44-45 0.19834482386215618 46-47 0.21823531557521722 48-49 0.2354258705070108 50-51 0.250720725145574 52-53 0.2707691918830709 54-55 0.2641198794909068 56-57 0.2350237522629922 58-59 0.2292217604564387 60-61 0.20726323205985356 62-63 0.19754058737411906 64-65 0.1888088769325731 66-67 0.18448610580937355 68-69 0.1905609635672254 70-71 0.1947544823977047 72-73 0.2309451243593754 74-75 0.24249166250905124 76-77 0.25920829236753723 78-79 0.24860960436447654 80-81 0.25334885509755245 82-83 0.2500744636819727 84-85 0.23610085470232764 86-87 0.22611970543115262 88-89 0.22830263304153908 90-91 0.2312467130423893 92-93 0.23809708455656267 94-95 0.24322409216779936 96-97 8.751557849161426 98-99 0.30163176711149564 100-101 0.3131208597977403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3481563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.874692681936565 #Duplication Level Percentage of deduplicated Percentage of total 1 76.89891808132315 43.73602333448669 2 12.400249528216031 14.105207621932312 3 4.463405086907499 7.615643778985691 4 2.126423077450657 4.837586361671356 5 1.1629780585659346 3.307200983838637 6 0.698793011525565 2.384618266728088 7 0.4524659569695745 1.8013703576178017 8 0.30882426903782406 1.4051428315399954 9 0.22441945190887636 1.1487408623249384 >10 1.1520020920408394 11.683683454094016 >50 0.07550988122990161 2.9339602442046484 >100 0.033861673934796735 3.4272526600899536 >500 0.0016891528447919228 0.645590727638452 >1k 3.071186963350386E-4 0.3108884980468104 >5k 1.0237289877834621E-4 0.36398903371844465 >10k+ 5.1186449389173105E-5 0.2931009830822443 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10068 0.289180462912778 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7404 0.21266310562238858 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5099 0.14645720901790374 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3648 0.10478052529855125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.872273171561164E-5 0.0 2 2.872273171561164E-5 0.0 0.0 2.872273171561164E-5 0.0 3 2.872273171561164E-5 0.0 0.0 2.872273171561164E-5 0.0 4 2.872273171561164E-5 0.0 0.0 5.744546343122328E-5 0.0 5 2.872273171561164E-5 0.0 0.0 8.616819514683491E-5 0.0 6 2.872273171561164E-5 0.0 0.0 8.616819514683491E-5 0.0 7 2.872273171561164E-5 0.0 0.0 8.616819514683491E-5 0.0 8 2.872273171561164E-5 0.0 0.0 8.616819514683491E-5 0.0 9 2.872273171561164E-5 0.0 0.0 1.436136585780582E-4 0.0 10-11 2.872273171561164E-5 0.0 0.0 3.015886830139222E-4 0.0 12-13 2.872273171561164E-5 0.0 0.0 4.595637074497862E-4 5.744546343122328E-5 14-15 2.872273171561164E-5 0.0 0.0 5.170091708810095E-4 5.744546343122328E-5 16-17 5.744546343122328E-5 0.0 0.0 6.175387318856502E-4 5.744546343122328E-5 18-19 1.1489092686244655E-4 0.0 0.0 7.467910246059025E-4 5.744546343122328E-5 20-21 1.436136585780582E-4 1.436136585780582E-5 0.0 0.0010052956100464073 5.744546343122328E-5 22-23 1.436136585780582E-4 2.872273171561164E-5 0.0 0.001493582049211805 5.744546343122328E-5 24-25 1.7233639029366982E-4 2.872273171561164E-5 0.0 0.0020967594152396496 5.744546343122328E-5 26-27 1.7233639029366982E-4 2.872273171561164E-5 0.0 0.0034610891717312023 5.744546343122328E-5 28-29 3.015886830139222E-4 2.872273171561164E-5 0.0 0.008099810343802482 5.744546343122328E-5 30-31 3.4467278058733964E-4 2.872273171561164E-5 0.0 0.025635038056183383 5.744546343122328E-5 32-33 3.4467278058733964E-4 2.872273171561164E-5 0.0 0.06445380996983252 5.744546343122328E-5 34-35 4.021182440185629E-4 2.872273171561164E-5 0.0 0.12592045584124142 5.744546343122328E-5 36-37 4.882864391653978E-4 2.872273171561164E-5 0.0 0.22775690113894248 5.744546343122328E-5 38-39 5.457319025966211E-4 2.872273171561164E-5 0.0 0.3894802420636938 5.744546343122328E-5 40-41 5.600932684544269E-4 2.872273171561164E-5 0.0 0.5650910237729434 5.744546343122328E-5 42-43 6.175387318856502E-4 2.872273171561164E-5 0.0 0.7404576622626102 5.744546343122328E-5 44-45 6.31900097743456E-4 2.872273171561164E-5 0.0 0.9448773438826183 5.744546343122328E-5 46-47 6.893455611746793E-4 2.872273171561164E-5 0.0 1.1929412163444981 5.744546343122328E-5 48-49 7.467910246059026E-4 2.872273171561164E-5 0.0 1.4602780417875536 7.18068292890291E-5 50-51 7.467910246059026E-4 2.872273171561164E-5 0.0 1.752158441481599 8.616819514683491E-5 52-53 7.467910246059026E-4 2.872273171561164E-5 0.0 2.0296200298544074 8.616819514683491E-5 54-55 7.467910246059026E-4 2.872273171561164E-5 0.0 2.319001551889195 8.616819514683491E-5 56-57 7.8987512217932E-4 2.872273171561164E-5 0.0 2.650907078228945 8.616819514683491E-5 58-59 8.042364880371259E-4 2.872273171561164E-5 0.0 3.0123826568699172 8.616819514683491E-5 60-61 8.185978538949317E-4 2.872273171561164E-5 0.0 3.3997948622500873 8.616819514683491E-5 62-63 8.329592197527375E-4 2.872273171561164E-5 0.0 3.772400499430859 8.616819514683491E-5 64-65 8.329592197527375E-4 2.872273171561164E-5 0.0 4.1423492839279366 8.616819514683491E-5 66-67 8.904046831839608E-4 2.872273171561164E-5 0.0 4.5506716379970715 8.616819514683491E-5 68-69 8.904046831839608E-4 2.872273171561164E-5 0.0 5.007635938226596 1.0052956100464073E-4 70-71 9.334887807573782E-4 2.872273171561164E-5 0.0 5.497344152611916 1.1489092686244655E-4 72-73 9.909342441886014E-4 2.872273171561164E-5 0.0 5.986880030606943 1.436136585780582E-4 74-75 0.0010052956100464073 2.872273171561164E-5 0.0 6.448483052008537 1.7233639029366982E-4 76-77 0.0010052956100464073 2.872273171561164E-5 0.0 6.939641764345497 1.7233639029366982E-4 78-79 0.0010052956100464073 2.872273171561164E-5 0.0 7.478049370354636 1.7233639029366982E-4 80-81 0.0011489092686244656 2.872273171561164E-5 0.0 8.05655390983877 1.8669775615147563E-4 82-83 0.0011489092686244656 2.872273171561164E-5 0.0 8.639151438592378 2.0105912200928146E-4 84-85 0.001191993366197883 2.872273171561164E-5 0.0 9.185831191335616 2.0105912200928146E-4 86-87 0.0012350774637713004 2.872273171561164E-5 0.0 9.674620278306037 2.0105912200928146E-4 88-89 0.001263800195486912 2.872273171561164E-5 0.0 10.173505405474495 2.0105912200928146E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6015 0.0 46.06234 1 GTATCAA 11110 0.0 43.10226 1 TATCAAC 14425 0.0 33.03438 2 ATCAACG 14470 0.0 32.868523 3 TCAACGC 14620 0.0 32.53176 4 CAACGCA 14870 0.0 31.986206 5 AACGCAG 15370 0.0 31.06855 6 GTACATG 15605 0.0 27.732588 1 TACATGG 15280 0.0 27.645573 2 ACGCAGA 17790 0.0 26.392672 7 CGCAGAG 17835 0.0 26.115427 8 ACATGGG 16055 0.0 25.433552 3 GCAGAGT 19395 0.0 22.680721 9 CATGGGA 8960 0.0 21.474304 4 TACCTGG 2415 0.0 21.036777 2 AGAGTAC 16700 0.0 20.451149 10-11 AGTACTT 10505 0.0 20.420645 12-13 GAGTACT 9795 0.0 20.243883 12-13 GAGTACA 11485 0.0 20.069263 1 CATGGGG 7630 0.0 19.915068 4 >>END_MODULE