FastQCFastQC Report
Fri 27 May 2016
SRR939832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481563
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113230.3252274912158706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91690.2633587271004431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62910.1806947052229128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.050.599021
GTATCAA121000.040.0454671
ATCAACG151200.031.2474563
TCAACGC151800.031.123954
TATCAAC155350.030.664922
CAACGCA155250.030.4323045
AACGCAG161000.029.4043646
TACATGG160900.026.0684762
GTACATG164200.025.8680021
ACGCAGA188100.025.0923547
CGCAGAG190900.024.649778
ACATGGG165350.024.3849493
GCAGAGT210150.021.91789
GAGTACT108100.021.43641912-13
CATGGGA93250.021.1108824
TGGTATC34850.020.4224512
AGAGTAC183150.020.05971510-11
GTACTTT115600.019.84047114-15
CAGAGTA196850.019.59139610-11
CATGGGG74500.019.5474114
Produced by FastQC (version 0.11.5)