##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939832_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3481563 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38684435697415 31.0 31.0 34.0 30.0 34.0 2 31.60623001795458 31.0 31.0 34.0 30.0 34.0 3 31.65315578089496 31.0 31.0 34.0 30.0 34.0 4 35.465275222651435 37.0 35.0 37.0 33.0 37.0 5 35.201439410977194 37.0 35.0 37.0 32.0 37.0 6 35.22503628399084 37.0 35.0 37.0 32.0 37.0 7 35.12905956319044 37.0 35.0 37.0 32.0 37.0 8 35.09430304722333 37.0 35.0 37.0 32.0 37.0 9 36.67536620764869 39.0 35.0 39.0 32.0 39.0 10-11 36.53574831189325 38.0 35.0 39.0 32.0 39.0 12-13 36.452866571709315 38.0 35.0 39.0 32.0 39.0 14-15 37.48538170930699 39.0 36.0 41.0 32.0 41.0 16-17 37.40827424349351 39.0 36.0 41.0 32.0 41.0 18-19 37.38684464420147 39.0 36.0 41.0 32.0 41.0 20-21 37.297214210973635 39.0 36.0 40.5 31.0 41.0 22-23 37.17041096197312 39.0 36.0 40.0 31.0 41.0 24-25 37.02056490145374 39.0 36.0 40.0 31.0 41.0 26-27 36.723589950835304 39.0 35.5 40.0 30.0 41.0 28-29 36.49536185328256 38.0 35.0 40.0 30.0 41.0 30-31 36.23049417747144 38.0 35.0 40.0 30.0 41.0 32-33 36.21967403720685 38.0 35.0 40.0 30.0 41.0 34-35 36.07736137476185 38.0 35.0 40.0 30.0 41.0 36-37 36.133086059335994 38.0 35.0 40.0 30.0 41.0 38-39 36.04300726426608 38.0 35.0 40.0 30.0 41.0 40-41 35.95789261891857 38.0 35.0 40.0 29.0 41.0 42-43 35.73777122516525 38.0 34.5 40.0 28.5 41.0 44-45 35.48366969088309 38.0 34.0 40.0 27.0 41.0 46-47 35.2052733212066 38.0 34.0 40.0 26.5 41.0 48-49 34.897601020001645 38.0 33.5 40.0 26.0 41.0 50-51 34.674029308100984 38.0 33.0 40.0 25.5 41.0 52-53 34.427555813294205 38.0 33.0 40.0 24.0 41.0 54-55 34.160103522469655 37.0 33.0 40.0 24.0 41.0 56-57 33.792454997942016 37.0 32.5 40.0 23.5 41.0 58-59 33.424275246491305 36.5 32.0 39.5 23.0 41.0 60-61 33.11056399094315 36.0 31.0 39.0 22.0 40.5 62-63 32.816412772079666 36.0 31.0 39.0 22.0 40.0 64-65 32.380804110108016 35.0 31.0 39.0 20.5 40.0 66-67 31.477346812336872 35.0 29.5 38.0 15.5 40.0 68-69 29.58706362630807 34.0 26.5 37.0 8.5 39.5 70-71 28.93427549637907 33.5 25.5 36.5 8.5 39.0 72-73 29.484614381529212 34.0 27.0 36.0 9.0 39.0 74-75 29.57698496336272 34.0 28.0 36.0 8.0 39.0 76-77 29.112538535134938 32.5 27.5 35.0 8.0 37.0 78-79 29.64537694707808 34.0 29.0 35.0 8.0 37.0 80-81 29.458036519804466 34.0 29.0 35.0 7.0 37.0 82-83 29.00871131730203 33.5 28.5 35.0 4.5 36.5 84-85 28.64894201253862 33.0 28.0 35.0 2.0 36.0 86-87 28.248554313105924 33.0 27.0 35.0 2.0 36.0 88-89 27.78495664160034 33.0 26.5 35.0 2.0 35.0 90-91 27.28281938313338 33.0 26.0 35.0 2.0 35.0 92-93 26.77938443164751 32.0 25.0 34.0 2.0 35.0 94-95 25.953157532981592 32.0 24.0 34.0 2.0 35.0 96-97 25.39960457415247 32.0 21.5 34.0 2.0 35.0 98-99 24.71156690256646 31.5 19.0 34.0 2.0 35.0 100-101 23.228404742352787 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2087973459557837 1101 2 0.23201953987280888 1101 3 0.24480765433999707 1101 4 0.1846517345418306 1101 5 0.21854842983163536 1101 6 0.14101022586470435 1101 7 0.11903627857265064 1101 8 0.10767056278847775 1101 9 0.26545631867671915 1101 10-11 0.1928631821168736 1101 12-13 0.16788927247737462 1101 14-15 0.2472220997027179 1101 16-17 0.27053539912910907 1101 18-19 0.192592158264965 1101 20-21 0.1850129675818124 1101 22-23 0.08538050508560957 1101 24-25 0.0670819661831743 1101 26-27 0.14341417150173186 1101 28-29 0.1205990211129091 1101 30-31 -0.0522018326894198 1101 32-33 0.07228814883665535 1101 34-35 0.04577835336001357 1101 36-37 0.21478063686585358 1101 38-39 0.11067392433474765 1101 40-41 0.1950221520368558 1101 42-43 -0.016867985392153173 1101 44-45 -0.10948465156931064 1101 46-47 -0.10110586057727033 1101 48-49 -0.05463206297505252 1101 50-51 0.039916122007255694 1101 52-53 -0.2445150073227893 1101 54-55 -0.41526565906445967 1101 56-57 -0.2865984462540965 1101 58-59 -0.2645630575180178 1101 60-61 -0.29324342069675424 1101 62-63 -0.23549328289438876 1101 64-65 -0.4136062168499919 1101 66-67 -0.506221502516226 1101 68-69 -9.200952775977367 1101 70-71 -6.940356601541048 1101 72-73 -2.0689569507596737 1101 74-75 -0.8922915673478258 1101 76-77 -0.7399870746176198 1101 78-79 -0.8434528201778058 1101 80-81 -0.7816336362843153 1101 82-83 -0.7344296559905743 1101 84-85 -0.4848640513338154 1101 86-87 -0.7235819177203666 1101 88-89 -0.6631996059739969 1101 90-91 -0.9105182291875629 1101 92-93 -1.27245451162009 1101 94-95 -0.8760278281556246 1101 96-97 -1.2499728924609883 1101 98-99 -1.1872097032057418 1101 100-101 -1.147828699498806 1102 1 0.20648277054552722 1102 2 0.13053780141023097 1102 3 0.22018518017932465 1102 4 0.09623224829552868 1102 5 0.30286809783144975 1102 6 0.2943800844547937 1102 7 0.2758478898514056 1102 8 0.1957099594453453 1102 9 0.2540435941849424 1102 10-11 0.3431242482441661 1102 12-13 0.28991614057843407 1102 14-15 0.41284581100918416 1102 16-17 0.35103300665934256 1102 18-19 0.35006336244202174 1102 20-21 0.3005641302152071 1102 22-23 0.3288032314756606 1102 24-25 0.23169261436465405 1102 26-27 0.31393497278818927 1102 28-29 0.08937254488377988 1102 30-31 0.0686775663239132 1102 32-33 0.056827535674905505 1102 34-35 -0.05516183380466089 1102 36-37 -0.06163438954622791 1102 38-39 0.2625472074403987 1102 40-41 0.288443023799168 1102 42-43 0.18865291408047824 1102 44-45 0.07769245228765698 1102 46-47 0.10362627663026558 1102 48-49 0.0741916884786491 1102 50-51 0.16466449266224714 1102 52-53 0.07673815372653081 1102 54-55 -0.2047379192883696 1102 56-57 -0.19594053343032414 1102 58-59 -0.24977491055295076 1102 60-61 -0.09332371516799753 1102 62-63 -0.012963919773554267 1102 64-65 -0.12675836396342532 1102 66-67 0.27153409245925886 1102 68-69 -4.783078092082302 1102 70-71 0.13566415283423794 1102 72-73 0.41053850322046515 1102 74-75 0.17997498411372703 1102 76-77 -0.09145844898404576 1102 78-79 -0.052269065843979945 1102 80-81 -0.11434381469950594 1102 82-83 0.030522898268198873 1102 84-85 -0.11264637476780237 1102 86-87 -0.27374261575860714 1102 88-89 -0.40252556879853074 1102 90-91 -0.6435971512342853 1102 92-93 -0.7012970275426156 1102 94-95 -0.3024394725994881 1102 96-97 -0.6653473199810449 1102 98-99 -0.32952816242465843 1102 100-101 -0.22108802227603164 1103 1 0.14124888294399085 1103 2 0.13760620642495525 1103 3 0.21066925239546563 1103 4 0.16184470192968092 1103 5 0.2708266423044705 1103 6 0.15774660597618606 1103 7 0.15693104705816552 1103 8 0.19190859626810663 1103 9 0.30449727926214365 1103 10-11 0.3860040662608597 1103 12-13 0.3989957837417606 1103 14-15 0.3541052645499647 1103 16-17 0.3817815233765103 1103 18-19 0.3095393964959854 1103 20-21 0.28358892374394173 1103 22-23 0.26121985782418733 1103 24-25 0.2860662828053222 1103 26-27 0.2670176122252599 1103 28-29 0.19589898933388383 1103 30-31 0.1788419794192464 1103 32-33 0.1900184823446267 1103 34-35 0.14862064351108728 1103 36-37 0.2660208776993258 1103 38-39 0.4438726978361984 1103 40-41 0.38634669317351467 1103 42-43 0.23510725273580135 1103 44-45 0.24759348188540997 1103 46-47 0.2970930417580604 1103 48-49 0.2772412246079412 1103 50-51 0.24871654973573953 1103 52-53 0.1630707316254032 1103 54-55 0.2814862240574456 1103 56-57 0.27682690310044933 1103 58-59 0.1340178372762324 1103 60-61 0.09573076099337641 1103 62-63 0.20695748109052658 1103 64-65 0.09412939884713012 1103 66-67 0.3628680284425556 1103 68-69 -3.8731564365773927 1103 70-71 0.6409534672562458 1103 72-73 0.8839317195887766 1103 74-75 0.5497887992275068 1103 76-77 0.3025006217466881 1103 78-79 0.08511051026392025 1103 80-81 0.19670144346385499 1103 82-83 0.10939640968035036 1103 84-85 0.07039688022103974 1103 86-87 0.004826812063452479 1103 88-89 -0.03784531279961456 1103 90-91 -0.11545106379062275 1103 92-93 -0.15317945987181503 1103 94-95 -0.13602426375156895 1103 96-97 -0.47295513915210563 1103 98-99 -0.28183733034331127 1103 100-101 -0.3206614988872758 1104 1 0.1728628196548705 1104 2 0.14616353985926622 1104 3 0.17792805750508478 1104 4 0.13277207634172328 1104 5 0.2548189834684109 1104 6 0.23217198747742884 1104 7 0.16554550895578757 1104 8 0.178013949715492 1104 9 0.1882161611247568 1104 10-11 0.1921033117370854 1104 12-13 0.21082730251924175 1104 14-15 0.21464958191421601 1104 16-17 0.17710600316998892 1104 18-19 0.18174524664819813 1104 20-21 0.25711457310688246 1104 22-23 0.30183407935420803 1104 24-25 0.1785095618144794 1104 26-27 0.12937263022813283 1104 28-29 0.16328900881024566 1104 30-31 0.06242915300909857 1104 32-33 0.15517098406095187 1104 34-35 0.2244568264077813 1104 36-37 0.23334940587583475 1104 38-39 0.25984627715668296 1104 40-41 0.1545658573857409 1104 42-43 0.15423429853571236 1104 44-45 0.22664717440915894 1104 46-47 0.26768143200308714 1104 48-49 0.2939164385927313 1104 50-51 0.09985743148970272 1104 52-53 -0.0343784084018921 1104 54-55 0.026087625433468986 1104 56-57 0.10166535467213578 1104 58-59 0.06351748577977645 1104 60-61 0.0018867645034035263 1104 62-63 0.20962934803883115 1104 64-65 0.15178593001342477 1104 66-67 0.2754834172371474 1104 68-69 -3.9865691131693772 1104 70-71 0.6892019118638615 1104 72-73 0.9060471437799684 1104 74-75 0.6614457633847479 1104 76-77 0.5357465885924242 1104 78-79 0.23908192627602531 1104 80-81 0.16625719969159647 1104 82-83 -0.019078877731793398 1104 84-85 0.11654988736785654 1104 86-87 -0.06439214141554217 1104 88-89 -0.14490080149493068 1104 90-91 -0.06263674611356151 1104 92-93 -0.28212041834584056 1104 94-95 -0.019591063717982138 1104 96-97 -0.3464417699392399 1104 98-99 -0.04983271396834965 1104 100-101 -0.12112980411461294 1105 1 0.2651174540565684 1105 2 0.2557951069880282 1105 3 0.2216388998821337 1105 4 0.21499136399896202 1105 5 0.21944531181631532 1105 6 0.2673988418351527 1105 7 0.251417598074525 1105 8 0.3156426612799308 1105 9 0.3535570377041495 1105 10-11 0.3063322156065382 1105 12-13 0.2631008709023561 1105 14-15 0.3099318020994417 1105 16-17 0.3356756132402481 1105 18-19 0.30172486853596325 1105 20-21 0.3348819388600148 1105 22-23 0.34229158031550355 1105 24-25 0.33184251474783366 1105 26-27 0.4308952425758221 1105 28-29 0.362916532052175 1105 30-31 0.3877273515961619 1105 32-33 0.4459474039212239 1105 34-35 0.49436759963965216 1105 36-37 0.28341803013848477 1105 38-39 0.4819161301326247 1105 40-41 0.4024260273764426 1105 42-43 0.43765823331293063 1105 44-45 0.3536160047449428 1105 46-47 0.25965204065533243 1105 48-49 0.270043521783478 1105 50-51 0.3311667564555165 1105 52-53 0.2143086106252099 1105 54-55 0.3315694161620826 1105 56-57 0.255579005266533 1105 58-59 0.34948335524669716 1105 60-61 0.299471315477291 1105 62-63 0.2872498163571535 1105 64-65 0.3970226624823354 1105 66-67 1.0053234072227895 1105 68-69 2.2740483737641277 1105 70-71 2.330058961025376 1105 72-73 1.4899478180335812 1105 74-75 1.0022869206961147 1105 76-77 0.6772460034870385 1105 78-79 0.6717226805450629 1105 80-81 0.5795722460440302 1105 82-83 0.5147324960244859 1105 84-85 0.5459591384922682 1105 86-87 0.5298215261525989 1105 88-89 0.5519240100508078 1105 90-91 0.6802859710898801 1105 92-93 0.5886529946449599 1105 94-95 0.985360173225736 1105 96-97 0.5792572991653842 1105 98-99 1.0008014614113172 1105 100-101 1.0480268795904486 1106 1 0.37945470367751355 1106 2 0.3223530743520229 1106 3 0.33484574228616637 1106 4 0.23308852823320336 1106 5 0.31270849437134274 1106 6 0.3165976042849934 1106 7 0.26209339318903346 1106 8 0.3302629222729365 1106 9 0.4359787139997522 1106 10-11 0.32375637797888857 1106 12-13 0.3643237994925741 1106 14-15 0.4784550807063255 1106 16-17 0.3689459140842004 1106 18-19 0.4542243381456146 1106 20-21 0.43022126171332786 1106 22-23 0.48195842964941704 1106 24-25 0.6308006560962482 1106 26-27 0.5694595353054126 1106 28-29 0.5753455232292666 1106 30-31 0.5559612295727945 1106 32-33 0.6605338755680705 1106 34-35 0.6566292004437244 1106 36-37 0.7618437988358764 1106 38-39 0.7525516319637973 1106 40-41 0.6205451854032802 1106 42-43 0.5811735458254716 1106 44-45 0.7116118746629212 1106 46-47 0.6226780179046614 1106 48-49 0.6081418901471451 1106 50-51 0.7357223384964584 1106 52-53 0.7903881290389734 1106 54-55 0.7959373014568811 1106 56-57 0.7790006332331316 1106 58-59 0.9257190009181002 1106 60-61 0.9069084120017763 1106 62-63 0.9306266130288421 1106 64-65 0.9793718452716433 1106 66-67 1.4403072170063922 1106 68-69 2.898686459149193 1106 70-71 2.992718702719138 1106 72-73 1.8847384019052917 1106 74-75 1.4205158019452 1106 76-77 1.0593709888449894 1106 78-79 0.9040628118852787 1106 80-81 0.9198840418433392 1106 82-83 1.044984036204319 1106 84-85 1.0475091889100696 1106 86-87 0.9291051101004868 1106 88-89 1.1286974592426873 1106 90-91 0.9860657513813109 1106 92-93 0.9728175868505531 1106 94-95 1.280193311195216 1106 96-97 1.2581821672231044 1106 98-99 1.3753695926106424 1106 100-101 1.6045267620444825 1107 1 0.06977362374072626 1107 2 0.12450267500450352 1107 3 0.05203261920311064 1107 4 0.07352921359790088 1107 5 0.03554990901501753 1107 6 0.0376848272976531 1107 7 0.04772615429109095 1107 8 0.08268936667690241 1107 9 0.12165389603231347 1107 10-11 0.07679021297870037 1107 12-13 0.08900871946716649 1107 14-15 0.15063330933298147 1107 16-17 0.05706842277762547 1107 18-19 0.07318805307040321 1107 20-21 0.08694830230778194 1107 22-23 0.08906816119139194 1107 24-25 0.2227990183365307 1107 26-27 0.12324484437102967 1107 28-29 0.1483386932132973 1107 30-31 0.10524438148896564 1107 32-33 0.21384155941809269 1107 34-35 0.1919145940229825 1107 36-37 0.07387485062879051 1107 38-39 0.19772311262658349 1107 40-41 0.02379855076974735 1107 42-43 0.14933589901377076 1107 44-45 0.11758243601408935 1107 46-47 0.09314911177389007 1107 48-49 0.2039362282247339 1107 50-51 0.15387878393428167 1107 52-53 0.055979379988727374 1107 54-55 0.055457231089000913 1107 56-57 0.1154224482581796 1107 58-59 0.18024187622025778 1107 60-61 0.2102371832389096 1107 62-63 0.15831931646469144 1107 64-65 0.021749616375849712 1107 66-67 0.5656829335262614 1107 68-69 1.9964752373771617 1107 70-71 2.2945375103709864 1107 72-73 1.323939116086681 1107 74-75 0.6634149730549836 1107 76-77 0.19542311817877334 1107 78-79 0.1972987657982621 1107 80-81 0.28110802927952605 1107 82-83 0.38789215099190955 1107 84-85 0.37601121313512564 1107 86-87 0.23446216797511 1107 88-89 0.41829280295068827 1107 90-91 0.46288599494473814 1107 92-93 0.4467767150635069 1107 94-95 0.7436629002291149 1107 96-97 0.37976543614554714 1107 98-99 0.483810824442255 1107 100-101 0.7436631063613071 1108 1 -0.1719920465564222 1108 2 -0.12865585746851949 1108 3 -0.11853536930147968 1108 4 -0.09819542503151268 1108 5 -0.06367286739825317 1108 6 0.00655247406041326 1108 7 -0.11089093632333658 1108 8 -0.04077713608118927 1108 9 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-0.7414773396932688 2316 64-65 -0.6329488378448715 2316 66-67 -0.14751313405295008 2316 68-69 1.271496504018966 2316 70-71 1.5952125362291127 2316 72-73 0.6949978070543033 2316 74-75 0.22561354051165594 2316 76-77 -0.294795467532154 2316 78-79 -0.4144778348771858 2316 80-81 -0.446061431884889 2316 82-83 -0.32555536243973293 2316 84-85 -0.4346857370640116 2316 86-87 -0.45775872402633766 2316 88-89 -0.3030621473837165 2316 90-91 -0.4457546990147918 2316 92-93 -0.4492683410549887 2316 94-95 -0.15326787817030763 2316 96-97 -0.2442259883254536 2316 98-99 -0.4391351375017791 2316 100-101 -0.6153095085328495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 30.0 4 48.0 5 146.0 6 370.0 7 886.0 8 2033.0 9 3387.0 10 5598.0 11 8457.0 12 11758.0 13 15193.0 14 18530.0 15 23093.0 16 27884.0 17 33229.0 18 30566.0 19 26666.0 20 26362.0 21 28146.0 22 30720.0 23 34841.0 24 42067.0 25 51182.0 26 63737.0 27 78994.0 28 97054.0 29 116842.0 30 139154.0 31 165344.0 32 197816.0 33 239191.0 34 295512.0 35 373289.0 36 467892.0 37 510905.0 38 291660.0 39 22970.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80172868256473 19.510911425272518 14.101122126774843 23.58623776538791 2 16.018504260047944 20.703982403600182 39.31744424703911 23.96006908931276 3 19.646842423027365 24.64994160666965 29.647526024238545 26.055689946064437 4 13.151937793456558 16.560406920684763 36.533792437477075 33.75386284838161 5 13.445512834321827 37.96636740452492 34.20943409612292 14.378685665030332 6 32.563506014545204 36.230626500764025 17.058502510368914 14.147364974321855 7 28.949468959774677 32.60954921683164 20.311107396304475 18.129874427089213 8 26.584180725725776 35.73211227256264 19.196464346616736 18.487242655094853 9 26.618705449247937 16.21082255297405 19.712353330960834 37.45811866681718 10-11 25.311835517553465 26.678218949362687 27.18530441643595 20.824641116647896 12-13 25.915630422313196 23.789372761601612 27.805973926078604 22.489022890006584 14-15 22.669875570253936 25.277569298616744 25.87370959537426 26.178845535755062 16-17 22.19092551122682 28.52754009239846 26.59311516718911 22.68841922918561 18-19 22.15721789322784 27.056684023813443 28.844903854963995 21.941194227994725 20-21 23.024500777380734 26.276028898514838 28.86377468970115 21.835695634403287 22-23 22.59108911715801 26.22439978825602 28.77971761533541 22.404793479250554 24-25 22.322303461335803 26.45441605854616 28.99406745911783 22.22921302100021 26-27 22.192484492530333 26.71173857146304 28.75265542798778 22.343121508018847 28-29 22.13412952814256 26.829007686209632 28.809211962453613 22.2276508231942 30-31 22.348262736236205 26.611648108135665 28.72014711827554 22.31994203735259 32-33 22.184436654671796 26.60763156443452 28.635758618154078 22.57217316273961 34-35 22.46557761855473 26.74246812255748 28.6374710085929 22.154483250294888 36-37 22.534247500889126 26.42773990788747 28.685543438915715 22.352469152307684 38-39 22.19425483687952 26.697473433978548 28.689635762298593 22.41863596684334 40-41 22.398609018665255 26.606183800443915 28.738384529573445 22.25682265131738 42-43 22.231056922817718 26.511955741073084 28.639438218464335 22.61754911764486 44-45 22.257179960366003 26.717585781900322 28.53961114064319 22.485623117090487 46-47 22.356622922594998 26.846179354287308 28.20195378591838 22.595243937199307 48-49 22.248572949750812 26.850407531513547 28.218811880049582 22.682207638686055 50-51 22.234251617643846 27.035278837297284 28.04865638105445 22.68181316400442 52-53 22.447624002276406 26.94636939269998 27.929822342685178 22.67618426233843 54-55 22.178711054954693 26.980372411333427 27.983773266008853 22.85714326770303 56-57 22.117813838814847 27.14448131380448 27.880605908936673 22.857098938444004 58-59 22.113231352427906 26.865353289420675 27.997847792198765 23.023567565952657 60-61 22.04062638690005 26.972769721090362 28.09110847156254 22.895495420447055 62-63 21.97881139533775 26.90504421377719 28.101628209038992 23.01451618184607 64-65 21.823200730170743 26.904063118693678 28.072508654979245 23.200227496156334 66-67 21.66925566343042 27.054498381877025 27.975073714491188 23.301172240201367 68-69 21.68918410329633 26.77492263070875 28.35661309528788 23.179280170707038 70-71 21.617589050196596 26.719787868896706 28.18722888175583 23.475394199150877 72-73 21.710589448892872 26.863363040408476 27.98006515942046 23.44598235127819 74-75 21.833055863575957 27.148106504141072 27.41991625197041 23.59892138031256 76-77 21.798878923992287 27.0129641023814 27.5391966459948 23.64896032763151 78-79 21.884334472823014 27.12465132391351 27.367686922122616 23.623327281140856 80-81 21.85811302306858 27.151490710713066 27.287351280428425 23.70304498578993 82-83 21.8747935553065 27.14023188442429 27.273433591014605 23.711540969254603 84-85 21.84613754039335 27.097097279669985 27.119659105319492 23.93710607461717 86-87 21.769970425142347 26.97519132892116 27.31514493231303 23.939693313623465 88-89 21.849163821192388 26.99007511592531 27.101878702371817 24.058882360510488 90-91 21.69929613075128 27.085918694348464 27.16586891180243 24.048916263097826 92-93 21.6813513013958 26.905044659969406 27.206827798631817 24.206776240002974 94-95 21.495962577724136 26.714738506428986 27.420020837103092 24.369278078743783 96-97 21.621213222440584 26.524011616332388 27.37123455275713 24.4835406084699 98-99 21.627035712243963 26.62257279496407 27.104990164919663 24.645401327872303 100-101 21.38439556752583 26.620942222487777 27.34596187079457 24.648700339191823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 797.0 1 558.5 2 418.5 3 559.0 4 518.0 5 510.0 6 877.0 7 1759.0 8 2737.5 9 3157.5 10 2967.5 11 2719.5 12 2828.5 13 2980.5 14 3169.5 15 3460.0 16 3794.5 17 4174.0 18 4576.5 19 5011.5 20 5568.5 21 6472.5 22 7682.0 23 9191.0 24 11094.5 25 13772.0 26 17854.5 27 22952.5 28 27084.0 29 31442.0 30 36735.5 31 42977.0 32 50962.5 33 57553.5 34 63621.0 35 71021.0 36 80512.5 37 88813.5 38 93699.0 39 97619.5 40 101980.5 41 107763.5 42 111868.5 43 115770.5 44 121192.0 45 125832.5 46 128792.5 47 131226.0 48 134521.5 49 135336.5 50 133115.5 51 130828.5 52 128159.0 53 125725.0 54 122998.5 55 115827.0 56 105592.0 57 95155.5 58 85777.0 59 75991.5 60 63616.5 61 51619.0 62 40800.5 63 30239.0 64 21713.0 65 15829.0 66 11346.5 67 8513.0 68 6229.0 69 4184.0 70 3003.5 71 2105.0 72 1505.5 73 1204.0 74 874.0 75 578.5 76 378.5 77 213.0 78 132.5 79 80.5 80 51.5 81 34.0 82 22.0 83 15.5 84 7.5 85 5.0 86 2.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021886721567296066 2 0.024443044689985503 3 2.872273171561164E-5 4 0.0 5 0.0 6 8.616819514683491E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.303114147295338E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.18068292890291E-5 26-27 4.3084097573417454E-4 28-29 8.616819514683491E-5 30-31 3.015886830139222E-4 32-33 0.002154204878670873 34-35 0.00412171200119027 36-37 0.09840407885768547 38-39 0.08922716607454755 40-41 0.09889236529685087 42-43 0.10009872002890656 44-45 0.09294675983171925 46-47 0.08715912939102351 48-49 0.087862836318056 50-51 0.08751816353746866 52-53 0.06873349699545865 54-55 0.05734493387021864 56-57 0.03176734127746647 58-59 0.013973608979645061 60-61 0.009421056002720618 62-63 0.0019818684883772027 64-65 0.005328066733245959 66-67 0.15260387360504463 68-69 0.40504796265355536 70-71 0.38830261006335376 72-73 0.17924420727127444 74-75 0.028823261276616278 76-77 0.030661516106415425 78-79 0.005170091708810095 80-81 0.001507943415069611 82-83 2.872273171561164E-5 84-85 5.313705367388153E-4 86-87 0.0016802798053632806 88-89 3.15950048871728E-4 90-91 6.175387318856502E-4 92-93 0.002570684488547242 94-95 0.0767615005099721 96-97 0.017291084492798207 98-99 0.018842112005441235 100-101 0.009966787905317239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3481563.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.90945826835998 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62184862639907 35.74607665122059 2 14.658465453668764 14.631921396761552 3 5.5271264489032434 8.275676605364552 4 2.713613458611331 5.417399106760888 5 1.493638980979319 3.7273356194591516 6 0.930569480734994 2.7866531198731557 7 0.5922377493748556 2.0690785666160254 8 0.4330362105345183 1.7290082142689025 9 0.3221448138720456 1.4470265829883904 >10 1.5438910997834743 13.809056418691542 >50 0.10772506260911885 3.701147902970385 >100 0.052133501559472314 4.568717834287616 >500 0.0027631842380027695 0.8914669436344148 >1k 6.332297177859614E-4 0.42993139375780787 >5k 1.1513267596108387E-4 0.44418199328359453 >10k+ 5.7566337980541934E-5 0.32532165006145797 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11323 0.3252274912158706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9169 0.2633587271004431 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6291 0.1806947052229128 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.872273171561164E-5 0.0 4 0.0 0.0 0.0 5.744546343122328E-5 0.0 5 0.0 0.0 0.0 1.1489092686244655E-4 0.0 6 2.872273171561164E-5 0.0 0.0 1.1489092686244655E-4 0.0 7 5.744546343122328E-5 0.0 0.0 1.1489092686244655E-4 0.0 8 5.744546343122328E-5 0.0 0.0 1.436136585780582E-4 0.0 9 5.744546343122328E-5 0.0 0.0 2.297818537248931E-4 0.0 10-11 5.744546343122328E-5 0.0 0.0 3.015886830139222E-4 0.0 12-13 8.616819514683491E-5 0.0 0.0 4.021182440185629E-4 2.872273171561164E-5 14-15 1.7233639029366982E-4 0.0 0.0 4.882864391653978E-4 2.872273171561164E-5 16-17 1.8669775615147563E-4 0.0 0.0 5.600932684544269E-4 2.872273171561164E-5 18-19 2.441432195826989E-4 0.0 0.0 6.749841953168736E-4 2.872273171561164E-5 20-21 2.872273171561164E-4 0.0 0.0 9.47850146615184E-4 2.872273171561164E-5 22-23 2.872273171561164E-4 0.0 0.0 0.0014361365857805818 2.872273171561164E-5 24-25 3.4467278058733964E-4 0.0 0.0 0.002154204878670873 2.872273171561164E-5 26-27 3.4467278058733964E-4 0.0 0.0 0.0035472573668780373 2.872273171561164E-5 28-29 5.457319025966212E-4 0.0 0.0 0.008473205856105434 2.872273171561164E-5 30-31 6.31900097743456E-4 0.0 0.0 0.026712140495518823 2.872273171561164E-5 32-33 6.31900097743456E-4 0.0 0.0 0.06759894909269198 2.872273171561164E-5 34-35 6.606228294590677E-4 0.0 0.0 0.13244051594068526 5.744546343122328E-5 36-37 6.893455611746793E-4 0.0 0.0 0.23976300299606812 5.744546343122328E-5 38-39 7.180682928902909E-4 0.0 0.0 0.41011752480136077 5.744546343122328E-5 40-41 7.180682928902909E-4 0.0 0.0 0.595594564854923 5.744546343122328E-5 42-43 7.467910246059025E-4 0.0 0.0 0.7800950320301543 5.744546343122328E-5 44-45 8.042364880371259E-4 0.0 0.0 0.9955873267265306 7.18068292890291E-5 46-47 8.904046831839608E-4 0.0 0.0 1.2540775508011777 8.616819514683491E-5 48-49 9.191274148995724E-4 0.0 0.0 1.5334204781013585 8.616819514683491E-5 50-51 9.191274148995724E-4 0.0 0.0 1.8375080387745388 8.616819514683491E-5 52-53 9.334887807573782E-4 0.0 0.0 2.126372551638445 8.616819514683491E-5 54-55 9.622115124729899E-4 0.0 0.0 2.428435159725675 1.1489092686244655E-4 56-57 0.001034018341762019 0.0 0.0 2.770436726263463 1.1489092686244655E-4 58-59 0.001034018341762019 0.0 0.0 3.1258374471465835 1.1489092686244655E-4 60-61 0.001034018341762019 0.0 0.0 3.493818724521142 1.1489092686244655E-4 62-63 0.001034018341762019 0.0 0.0 3.8409042145725927 1.1489092686244655E-4 64-65 0.001034018341762019 0.0 0.0 4.19652035594358 1.1489092686244655E-4 66-67 0.001120186536908854 0.0 0.0 4.568350479368031 1.1489092686244655E-4 68-69 0.0012063547320556888 0.0 0.0 4.9919246039781555 1.1489092686244655E-4 70-71 0.0012494388296291061 0.0 0.0 5.476649424410818 1.1489092686244655E-4 72-73 0.0013068842930603294 0.0 0.0 5.978277572458118 1.1489092686244655E-4 74-75 0.0013212456589181353 0.0 0.0 6.465070429574303 1.292522927202524E-4 76-77 0.0013212456589181353 0.0 0.0 6.986632153432237 1.436136585780582E-4 78-79 0.0013212456589181353 0.0 0.0 7.562020276525227 1.436136585780582E-4 80-81 0.001507943415069611 0.0 0.0 8.182560533875158 1.436136585780582E-4 82-83 0.00157975024435864 0.0 0.0 8.807538453275154 1.436136585780582E-4 84-85 0.0016515570736476692 0.0 0.0 9.392606136956303 1.436136585780582E-4 86-87 0.0017377252687945039 0.0 0.0 9.989536308836003 1.57975024435864E-4 88-89 0.0017520866346523098 0.0 0.0 10.634849922290648 1.7233639029366982E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6800 0.0 50.59902 1 GTATCAA 12100 0.0 40.045467 1 ATCAACG 15120 0.0 31.247456 3 TCAACGC 15180 0.0 31.12395 4 TATCAAC 15535 0.0 30.66492 2 CAACGCA 15525 0.0 30.432304 5 AACGCAG 16100 0.0 29.404364 6 TACATGG 16090 0.0 26.068476 2 GTACATG 16420 0.0 25.868002 1 ACGCAGA 18810 0.0 25.092354 7 CGCAGAG 19090 0.0 24.64977 8 ACATGGG 16535 0.0 24.384949 3 GCAGAGT 21015 0.0 21.9178 9 GAGTACT 10810 0.0 21.436419 12-13 CATGGGA 9325 0.0 21.110882 4 TGGTATC 3485 0.0 20.422451 2 AGAGTAC 18315 0.0 20.059715 10-11 GTACTTT 11560 0.0 19.840471 14-15 CAGAGTA 19685 0.0 19.591396 10-11 CATGGGG 7450 0.0 19.547411 4 >>END_MODULE