FastQCFastQC Report
Fri 27 May 2016
SRR939831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487235
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88550.35601782702478857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64170.2579973343893922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42600.17127452773863347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131050.034.1344341
GGTATCA83350.032.95271
TATCAAC156800.029.1937942
ATCAACG161850.028.344373
TCAACGC163650.028.1496964
CAACGCA163750.028.0461525
AACGCAG168050.027.4691266
TACATGG135750.026.9066832
GTACATG136200.026.5745221
ACATGGG143700.024.5292933
ACGCAGA188650.024.0420557
CGCAGAG189850.023.6397178
GAGTACT81100.022.86994712-13
GCAGAGT194000.021.9602039
GTACTTT89300.020.98492214-15
CATGGGA94300.020.8285564
AGTACTT85300.020.52043712-13
CATGGGG55100.020.148134
GTATAGG6050.019.6021021
AGAGTAC164800.019.08402410-11
Produced by FastQC (version 0.11.5)