FastQCFastQC Report
Fri 27 May 2016
SRR939831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487235
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97270.3910768383365464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78230.31452596960078155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.21976210530971138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79850.035.6689951
GTATCAA126400.034.4709931
TATCAAC159250.027.208452
ATCAACG158650.027.1924723
TCAACGC159150.027.0467074
CAACGCA162300.026.492725
AACGCAG166250.025.903316
GTACATG144600.024.3276061
TACATGG141850.024.1309812
ACATGGG146850.022.7314433
ACGCAGA192100.022.368577
CGCAGAG193550.022.1527548
GAGTACT86600.021.92658612-13
AGTACTT89650.020.99666812-13
CATGGGG54800.020.9922754
GCAGAGT202700.020.7576039
GTACTTT93050.020.35605214-15
GGACCGA4400.019.2748326
AGAGTAC175850.019.23807110-11
CATGGGA92000.018.5511554
Produced by FastQC (version 0.11.5)