FastQCFastQC Report
Fri 27 May 2016
SRR939830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725207
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96880.3554959311347725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71410.26203514081682605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47320.17363818601669523No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27740.1017904328001506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147900.034.2229231
GGTATCA94200.033.208081
TATCAAC178450.028.2069552
ATCAACG180650.027.9936493
TCAACGC183600.027.4162524
CAACGCA185750.027.2290485
GTACATG154150.026.7321341
AACGCAG189900.026.6597526
TACATGG154750.026.1430822
ACATGGG161300.024.2940333
ACGCAGA212800.023.5476077
CGCAGAG215000.023.2186938
GAGTACT93700.021.86761512-13
GCAGAGT218050.021.55619
AGTACTT96400.020.98670412-13
CATGGGA101650.020.825824
CATGGGG61300.020.4902734
GTACTTT100700.020.42229814-15
ATGGGAG41200.019.6419265
TAAGGTG10550.019.1764775
Produced by FastQC (version 0.11.5)