FastQCFastQC Report
Fri 27 May 2016
SRR939830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725207
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104660.38404422122796544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84990.3118662178689545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.20926850694277535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94500.035.502521
GTATCAA144650.033.39651
TATCAAC179300.026.8317852
ATCAACG180850.026.4920223
TCAACGC182250.026.2885154
CAACGCA184400.025.956285
GTACATG159050.025.6588041
AACGCAG189400.025.321156
TACATGG158550.025.1664312
ACATGGG162200.023.7182253
GAGTACT95400.022.54971712-13
ACGCAGA218300.021.7512687
CGCAGAG217800.021.7140838
GACGCGT1553.95007E-521.4225228
AGTACTT98500.021.28620512-13
GTACTTT102000.021.09061814-15
TAAGGTG11450.020.7146075
CATGGGA103950.020.5808944
GTAAGGT9900.020.1245564
GCAGAGT231400.020.0483999
Produced by FastQC (version 0.11.5)