##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939830_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2725207 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.392058291351812 31.0 31.0 34.0 30.0 34.0 2 31.606851149288843 31.0 31.0 34.0 30.0 34.0 3 31.648168744612793 31.0 31.0 34.0 30.0 34.0 4 35.46280484381553 37.0 35.0 37.0 33.0 37.0 5 35.194190753216176 37.0 35.0 37.0 32.0 37.0 6 35.22782159300193 37.0 35.0 37.0 32.0 37.0 7 35.133917900548475 37.0 35.0 37.0 32.0 37.0 8 35.09375177738792 37.0 35.0 37.0 32.0 37.0 9 36.662545267203555 39.0 35.0 39.0 32.0 39.0 10-11 36.53010908896095 38.0 35.0 39.0 32.0 39.0 12-13 36.447477200814475 38.0 35.0 39.0 32.0 39.0 14-15 37.450857861439516 39.0 36.0 41.0 32.0 41.0 16-17 37.37874077088456 39.0 36.0 41.0 32.0 41.0 18-19 37.3765231411779 39.0 36.0 41.0 32.0 41.0 20-21 37.301330871379676 39.0 36.0 40.5 31.0 41.0 22-23 37.18199388156569 39.0 36.0 40.0 31.0 41.0 24-25 37.022165472200825 39.0 36.0 40.0 31.0 41.0 26-27 36.732806902374755 39.0 35.5 40.0 30.0 41.0 28-29 36.497818330864405 38.0 35.0 40.0 30.0 41.0 30-31 36.22710990394491 38.0 35.0 40.0 30.0 41.0 32-33 36.21698626929991 38.0 35.0 40.0 30.0 41.0 34-35 36.06781888495075 38.0 35.0 40.0 30.0 41.0 36-37 36.1101257629237 38.0 35.0 40.0 30.0 41.0 38-39 36.01687614922463 38.0 35.0 40.0 30.0 41.0 40-41 35.92516348299414 38.0 35.0 40.0 29.0 41.0 42-43 35.69492611020007 38.0 34.5 40.0 28.5 41.0 44-45 35.43013338069365 38.0 34.0 40.0 27.0 41.0 46-47 35.125441847169775 38.0 34.0 40.0 26.5 41.0 48-49 34.813749010625614 38.0 33.5 40.0 25.5 41.0 50-51 34.58631894751481 38.0 33.0 40.0 24.5 41.0 52-53 34.33942284017324 38.0 33.0 40.0 24.0 41.0 54-55 34.07515777700556 37.5 33.0 40.0 24.0 41.0 56-57 33.71690278940278 37.0 32.5 40.0 23.0 41.0 58-59 33.35446426638417 37.0 32.0 39.5 22.5 41.0 60-61 33.040207954845265 36.0 31.0 39.0 22.0 41.0 62-63 32.74713443786105 36.0 31.0 39.0 21.0 40.0 64-65 32.30476547286133 35.5 31.0 39.0 19.5 40.0 66-67 31.41433990151941 35.0 29.5 38.0 14.5 40.0 68-69 29.568951459467115 34.0 26.5 37.0 7.5 39.5 70-71 28.91102198842143 33.5 25.5 36.5 7.5 39.0 72-73 29.455021031429908 34.0 27.0 36.0 7.5 39.0 74-75 29.525802260158585 34.0 28.0 36.0 8.0 39.0 76-77 29.045951371767355 32.5 27.5 35.0 8.0 37.0 78-79 29.57274419888104 34.0 29.0 35.0 7.0 37.0 80-81 29.379727668393627 34.0 29.0 35.0 7.0 37.0 82-83 28.93860723240473 33.5 28.5 35.0 2.0 36.5 84-85 28.58556487635618 33.0 28.0 35.0 2.0 36.0 86-87 28.209740948118807 33.0 27.0 35.0 2.0 36.0 88-89 27.762959107326527 33.0 26.5 35.0 2.0 35.0 90-91 27.27740553286411 33.0 26.0 35.0 2.0 35.0 92-93 26.786142850799955 32.0 25.0 34.5 2.0 35.0 94-95 25.987891745471078 32.0 24.0 34.0 2.0 35.0 96-97 25.466713904668524 32.0 23.0 34.0 2.0 35.0 98-99 24.799544218108935 31.5 19.0 34.0 2.0 35.0 100-101 23.338000012476115 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3006211112713828 1101 2 0.3283013027922621 1101 3 0.39562382362075965 1101 4 0.3169450816082957 1101 5 0.26380508554989035 1101 6 0.2821631082237701 1101 7 0.24620939746731096 1101 8 0.2457462871961269 1101 9 0.2722794128736865 1101 10-11 0.39640217495605157 1101 12-13 0.31067185885366655 1101 14-15 0.42341531998747683 1101 16-17 0.5233942087879413 1101 18-19 0.3299271797576395 1101 20-21 0.4136815338295321 1101 22-23 0.4546541063189977 1101 24-25 0.3817142395803117 1101 26-27 0.36357800150019415 1101 28-29 0.40085030562421053 1101 30-31 0.17535512763217298 1101 32-33 0.12601683970047617 1101 34-35 0.21845195026164532 1101 36-37 0.38497879226569154 1101 38-39 0.33494403103825476 1101 40-41 0.45672568483352194 1101 42-43 0.2258447417201097 1101 44-45 0.08946120898901455 1101 46-47 0.017750493857469962 1101 48-49 0.21433647866005856 1101 50-51 0.14297413839496897 1101 52-53 0.025054655534511028 1101 54-55 -0.03290831188522958 1101 56-57 -0.105553220151819 1101 58-59 -0.10918801391358812 1101 60-61 -0.17817757500500164 1101 62-63 -0.24099830895726626 1101 64-65 -0.2607175996997384 1101 66-67 -0.4537578843039576 1101 68-69 -8.792764520643704 1101 70-71 -6.746901795031501 1101 72-73 -1.7802015622907241 1101 74-75 -0.6698705429831975 1101 76-77 -0.5137679150055696 1101 78-79 -0.49708425304814696 1101 80-81 -0.5439852752410275 1101 82-83 -0.4384155092621036 1101 84-85 -0.46203436794126773 1101 86-87 -0.4955355612351511 1101 88-89 -0.3618332672375395 1101 90-91 -0.7503397635464637 1101 92-93 -0.9741948819925099 1101 94-95 -0.7135276563113813 1101 96-97 -1.0544813701446536 1101 98-99 -0.9277653498078458 1101 100-101 -1.0032949541583243 1102 1 0.2314740277559224 1102 2 0.1386026300754608 1102 3 0.2274665478469764 1102 4 0.18073710125736397 1102 5 0.25346199012842874 1102 6 0.25140371906545056 1102 7 0.26311966535232756 1102 8 0.1386251665333873 1102 9 0.3116451199832895 1102 10-11 0.31460295342533584 1102 12-13 0.26249911082933153 1102 14-15 0.40710624964076914 1102 16-17 0.4561796995635632 1102 18-19 0.34097512985790246 1102 20-21 0.39161137445841376 1102 22-23 0.38282088746643694 1102 24-25 0.28986534033032996 1102 26-27 0.3433533416468677 1102 28-29 0.28423546752777895 1102 30-31 0.13491940685426584 1102 32-33 0.1717240186859712 1102 34-35 0.12708081745699928 1102 36-37 0.22352176418668535 1102 38-39 0.4005560175225895 1102 40-41 0.34264857335698906 1102 42-43 0.2693305079434225 1102 44-45 0.31475403694174275 1102 46-47 0.24201799033631488 1102 48-49 0.21994104123941582 1102 50-51 0.22183217529248367 1102 52-53 0.35489729650198143 1102 54-55 0.08835756798627159 1102 56-57 0.14818266922506496 1102 58-59 0.014149135445663319 1102 60-61 0.08852128504398848 1102 62-63 0.24118431910509486 1102 64-65 0.049544665354481765 1102 66-67 0.40508197443370264 1102 68-69 -4.514893863107748 1102 70-71 0.2684769281956072 1102 72-73 0.6538429395097474 1102 74-75 0.45452898799252495 1102 76-77 0.14080854080831529 1102 78-79 0.09308399900383435 1102 80-81 0.06530730293546583 1102 82-83 0.0667472268978635 1102 84-85 0.07575157381923603 1102 86-87 -0.14461326246113515 1102 88-89 -0.14138935934947128 1102 90-91 -0.15220871427810323 1102 92-93 -0.46606723245632864 1102 94-95 -0.2092587721867396 1102 96-97 -0.7397301403435712 1102 98-99 -0.40163396750449465 1102 100-101 -0.45315292861176104 1103 1 0.197300371561699 1103 2 0.17296556044271938 1103 3 0.15617908585840468 1103 4 0.21822400865898572 1103 5 0.327710571661477 1103 6 0.23759895959615562 1103 7 0.207698553465967 1103 8 0.36325412106999977 1103 9 0.4813115780398576 1103 10-11 0.34469305337844247 1103 12-13 0.3894812471601128 1103 14-15 0.3759239423913243 1103 16-17 0.35898577768831785 1103 18-19 0.3365770244515218 1103 20-21 0.3175833858464543 1103 22-23 0.2633104512425746 1103 24-25 0.314100468418566 1103 26-27 0.2699137369929545 1103 28-29 0.35185468710815115 1103 30-31 0.3500447553774606 1103 32-33 0.36778986648992884 1103 34-35 0.19362580397903884 1103 36-37 0.2218281007591898 1103 38-39 0.3588115305754229 1103 40-41 0.4308634101645268 1103 42-43 0.322172180506179 1103 44-45 0.3482630548348311 1103 46-47 0.22681927424158488 1103 48-49 0.1037065460622344 1103 50-51 -0.005312900229277773 1103 52-53 0.09749354446149283 1103 54-55 0.016184330101587818 1103 56-57 0.07504277137320514 1103 58-59 0.170579609731341 1103 60-61 0.07989909753564461 1103 62-63 -0.0038376339756140965 1103 64-65 -0.043275933775895226 1103 66-67 0.3629186332734058 1103 68-69 -3.8592247067367786 1103 70-71 0.47642415583279885 1103 72-73 0.6659236699827815 1103 74-75 0.5103964601229265 1103 76-77 0.3295223805903156 1103 78-79 0.14858082055941324 1103 80-81 0.16335813299122393 1103 82-83 0.2567775434459847 1103 84-85 0.23694739987436364 1103 86-87 0.056663888850902566 1103 88-89 0.013396880663254507 1103 90-91 -0.08502877418133536 1103 92-93 -0.025200977672053426 1103 94-95 0.07973061811076576 1103 96-97 -0.4471633320072286 1103 98-99 -0.18646722376647418 1103 100-101 -0.34453692315195283 1104 1 0.22493231408725833 1104 2 0.2611508934923208 1104 3 0.21806775559740288 1104 4 0.22917647430698196 1104 5 0.3149397971518937 1104 6 0.26687902574342814 1104 7 0.24043658253561517 1104 8 0.24660682852395865 1104 9 0.2951389832098883 1104 10-11 0.24931838647039228 1104 12-13 0.2183405349335601 1104 14-15 0.31556323437838074 1104 16-17 0.3391483456168416 1104 18-19 0.2539422975578063 1104 20-21 0.32700974248484016 1104 22-23 0.2723268281973219 1104 24-25 0.1905019160782686 1104 26-27 0.34296647906624145 1104 28-29 0.2768935147515279 1104 30-31 0.292958194347392 1104 32-33 0.28328707206416937 1104 34-35 0.2358169217387669 1104 36-37 0.22954043000490287 1104 38-39 0.21717029905892105 1104 40-41 0.09960366355262096 1104 42-43 0.17168687466504906 1104 44-45 0.26230945108958537 1104 46-47 0.3440802129441991 1104 48-49 0.33138237150034655 1104 50-51 0.3326343587561311 1104 52-53 0.17521830808134098 1104 54-55 0.11585293206292846 1104 56-57 0.30285900625106876 1104 58-59 0.33684595730730393 1104 60-61 0.13414134179989645 1104 62-63 0.34395840099944763 1104 64-65 0.28840358544947975 1104 66-67 0.3646767288502808 1104 68-69 -3.7090494662277145 1104 70-71 0.7485838356410461 1104 72-73 0.9179764798125554 1104 74-75 0.5817497318613789 1104 76-77 0.6372556719993234 1104 78-79 0.06458672913685248 1104 80-81 0.11583886738685223 1104 82-83 0.12330348306436534 1104 84-85 0.19536000300450596 1104 86-87 0.1053096060586114 1104 88-89 0.14993784845880853 1104 90-91 0.1428099579677422 1104 92-93 -0.18707744594984277 1104 94-95 0.2504074895396542 1104 96-97 -0.1277296068508349 1104 98-99 0.33875512867524904 1104 100-101 0.24624962379747117 1105 1 0.22865016486393586 1105 2 0.2286621404153344 1105 3 0.23271846436576027 1105 4 0.14929785619306557 1105 5 0.25359101521487304 1105 6 0.19927440813881248 1105 7 0.19347112968780777 1105 8 0.19403489065790325 1105 9 0.3311715780660123 1105 10-11 0.2365987531625251 1105 12-13 0.24432304915136882 1105 14-15 0.2833503492243139 1105 16-17 0.35717100922774847 1105 18-19 0.2678990923969593 1105 20-21 0.33222152416721684 1105 22-23 0.3788415553002835 1105 24-25 0.2867649667530898 1105 26-27 0.30971590610977984 1105 28-29 0.4336798656555274 1105 30-31 0.5012787953150593 1105 32-33 0.3565087849917674 1105 34-35 0.29610467320298284 1105 36-37 0.1640578733458753 1105 38-39 0.4324321613796016 1105 40-41 0.4437022120629308 1105 42-43 0.29407037351913345 1105 44-45 0.36759338284410603 1105 46-47 0.4342147516081454 1105 48-49 0.3523950189835787 1105 50-51 0.2737492895769478 1105 52-53 0.32229623015926023 1105 54-55 0.41164064108593124 1105 56-57 0.20220328941887544 1105 58-59 0.16320407631248202 1105 60-61 0.17474061181093248 1105 62-63 0.258288678565016 1105 64-65 0.2735173279161671 1105 66-67 0.7686343725138052 1105 68-69 2.054516869397446 1105 70-71 2.1355437711303935 1105 72-73 1.337465487536857 1105 74-75 0.8026541542363859 1105 76-77 0.45147772445666945 1105 78-79 0.4038300022036587 1105 80-81 0.35512245961162137 1105 82-83 0.34867696579079066 1105 84-85 0.23933334991902555 1105 86-87 0.3200664178742514 1105 88-89 0.5083115009501071 1105 90-91 0.3828426377063039 1105 92-93 0.3511727025944218 1105 94-95 0.7738923380245062 1105 96-97 0.5468353706553195 1105 98-99 0.8555958043394547 1105 100-101 0.8411227245529211 1106 1 0.43117707155696294 1106 2 0.34944153133752565 1106 3 0.29910723084623214 1106 4 0.2694527656968546 1106 5 0.3804107651841093 1106 6 0.2836977057024228 1106 7 0.2553245651296123 1106 8 0.35280482503375765 1106 9 0.4642063671993242 1106 10-11 0.439324556988808 1106 12-13 0.46034617838686387 1106 14-15 0.5609666802272599 1106 16-17 0.6556971457402554 1106 18-19 0.5751993317881201 1106 20-21 0.5647739579773443 1106 22-23 0.5637785712080898 1106 24-25 0.5844087701744343 1106 26-27 0.6356695999987565 1106 28-29 0.6327878060578485 1106 30-31 0.7017345516204045 1106 32-33 0.7554285472680036 1106 34-35 0.7102285531659831 1106 36-37 0.8771965971488171 1106 38-39 0.9001848222738857 1106 40-41 0.8040442674921096 1106 42-43 0.6375561946546284 1106 44-45 0.76686984985934 1106 46-47 0.7217878492821228 1106 48-49 0.7221682127316242 1106 50-51 0.7621068597765159 1106 52-53 0.6837762868509074 1106 54-55 0.8862417550686814 1106 56-57 0.9124736863000464 1106 58-59 0.8844163960247329 1106 60-61 0.9649132672266134 1106 62-63 0.9908131238563911 1106 64-65 0.9515491196974679 1106 66-67 1.3052304525331841 1106 68-69 2.8640964858157894 1106 70-71 3.1702957849142415 1106 72-73 2.1343551889529557 1106 74-75 1.5664443554758236 1106 76-77 1.153429179264144 1106 78-79 0.9444203018991573 1106 80-81 1.029672559060188 1106 82-83 1.0817806344702454 1106 84-85 0.9939030214807083 1106 86-87 0.9732412290013741 1106 88-89 1.091906854458994 1106 90-91 1.1287860889884165 1106 92-93 0.9747271253758889 1106 94-95 1.241001064487353 1106 96-97 1.3092676324849357 1106 98-99 1.4625085714710089 1106 100-101 1.72075204540457 1107 1 0.08746364647231886 1107 2 0.08834838185641658 1107 3 0.11134176267804818 1107 4 0.12869057458811284 1107 5 0.12512603339948924 1107 6 0.11610377926714932 1107 7 0.03566808825588197 1107 8 0.05831946479955974 1107 9 0.049333237047385126 1107 10-11 -0.03558778239938931 1107 12-13 0.02952562367840983 1107 14-15 0.17204199167768053 1107 16-17 0.07872564606642385 1107 18-19 -0.015789125536080917 1107 20-21 0.1334367360327846 1107 22-23 0.0751191621170193 1107 24-25 0.044576593333466974 1107 26-27 -0.006337876347636495 1107 28-29 0.10149007409656718 1107 30-31 0.09492381405293315 1107 32-33 -0.05512746163220328 1107 34-35 -0.015079613050033913 1107 36-37 -0.18178332175766343 1107 38-39 0.006444726221317865 1107 40-41 -0.10025158584776506 1107 42-43 -0.07374435007406532 1107 44-45 -0.0035748905171928413 1107 46-47 0.015602594910752998 1107 48-49 0.16699307823127896 1107 50-51 0.1475420746181797 1107 52-53 0.07214325553251655 1107 54-55 -0.06874668265619732 1107 56-57 -0.031004255591142282 1107 58-59 0.13483804628226181 1107 60-61 0.1413447658780953 1107 62-63 0.14965456296567226 1107 64-65 -0.01451790044560397 1107 66-67 0.5127717018203874 1107 68-69 2.036698171198484 1107 70-71 2.2427254067983142 1107 72-73 1.1938569397814902 1107 74-75 0.6348394306431899 1107 76-77 0.21956119499339266 1107 78-79 0.1829652933324013 1107 80-81 0.12338386529931356 1107 82-83 0.36046119493667206 1107 84-85 0.36101803585839676 1107 86-87 0.3388005472734221 1107 88-89 0.27338113922282403 1107 90-91 0.2100922168750472 1107 92-93 0.020541465245596413 1107 94-95 0.38322108752450035 1107 96-97 0.12673041562560172 1107 98-99 0.3107373225625416 1107 100-101 0.432583427148753 1108 1 -0.11142355656336989 1108 2 -0.2002193792567759 1108 3 -0.14112641717397523 1108 4 -0.10554491678092859 1108 5 -0.017369401056001266 1108 6 0.039731032063599514 1108 7 -0.060796693899909826 1108 8 -0.1292867994801199 1108 9 -0.03890772983787372 1108 10-11 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-0.7115728041563827 2316 62-63 -0.6206247357991899 2316 64-65 -0.5451965983322999 2316 66-67 0.025767487701330083 2316 68-69 1.1562705446734114 2316 70-71 1.5207062182355848 2316 72-73 0.6309606976393844 2316 74-75 0.171744718042806 2316 76-77 -0.23006244168682954 2316 78-79 -0.4119422234816881 2316 80-81 -0.44193010563635937 2316 82-83 -0.24777455780786184 2316 84-85 -0.2859290200520732 2316 86-87 -0.3790016839427466 2316 88-89 -0.425475573201588 2316 90-91 -0.4798569567693889 2316 92-93 -0.5491550710419943 2316 94-95 -0.3450232097046033 2316 96-97 -0.28598022091258457 2316 98-99 -0.5237719057646224 2316 100-101 -0.5245524823557268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 58.0 5 141.0 6 275.0 7 730.0 8 1575.0 9 2715.0 10 4502.0 11 6921.0 12 9639.0 13 12360.0 14 15510.0 15 19405.0 16 23930.0 17 28141.0 18 25290.0 19 21127.0 20 21030.0 21 21740.0 22 23243.0 23 27262.0 24 32189.0 25 39614.0 26 48919.0 27 60532.0 28 74393.0 29 89421.0 30 107528.0 31 127312.0 32 152968.0 33 184910.0 34 229754.0 35 290256.0 36 364871.0 37 406490.0 38 233335.0 39 17104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79099948946653 19.972282180032966 14.52792537329319 22.708792957207315 2 15.676151437250322 21.265270647030583 39.41463926697958 23.643938648739514 3 19.575283639004304 25.595266708180333 29.71965065406041 25.109798998754957 4 13.17371487743867 17.330096392677692 36.58470714334728 32.91148158653636 5 13.538751368244686 38.22828871348121 33.55888194915102 14.67407796912308 6 31.509874849827867 36.49834177673174 17.44895615230555 14.542827221134843 7 28.47567175631062 33.17318647721072 20.3947076313836 17.95643413509506 8 26.700283684872378 34.92883292902154 19.5453409594207 18.825542426685384 9 26.64619605042846 16.67906327849591 20.128415933174985 36.54632473790064 10-11 25.121486184352232 27.11973806026478 26.87061936946441 20.888156385918574 12-13 26.19599171732643 24.340646416951078 27.519304038188658 21.944057827533836 14-15 22.87308817275165 25.373980765497812 26.178506807005853 25.574424254744688 16-17 22.124849001686485 29.031930180434728 26.587500495377213 22.255720322501574 18-19 22.04093524655838 27.103891026239662 29.281432434569638 21.57374129263232 20-21 22.923488013938023 26.200248274718213 29.198515929248675 21.677747782095086 22-23 22.620795411137575 26.38162899185273 28.94725061252228 22.050324984487418 24-25 22.463470172605398 26.551255144338707 29.050553820823353 21.934720862232542 26-27 22.162501834727728 26.83507632467342 28.948444150888008 22.053977689710848 28-29 21.93609288108601 27.015894217132292 29.14909153623305 21.898921365548645 30-31 22.04775984125921 26.64146360034456 29.093322594050523 22.217453964345705 32-33 21.83240191483424 26.751297041312856 28.926447753504448 22.489853290348456 34-35 22.55539949561345 26.691259104048758 28.74845532766319 22.004886072674598 36-37 22.42495812641415 26.2594582880315 28.88610751961447 22.42947606593988 38-39 22.046695918262035 26.662297230268255 29.061294108551415 22.229712742918288 40-41 22.348948027461958 26.47084967733191 29.025472211315 22.154730083891135 42-43 22.13081484615938 26.447696688397272 28.816942082582166 22.604546382861184 44-45 22.112284498432015 26.638028621149918 28.739235481352033 22.51045139906604 46-47 22.321855692253166 26.802772556865605 28.202125969720036 22.673245781161196 48-49 22.254089682967738 26.949294193284658 28.046609742297772 22.750006381449833 50-51 22.044375592689473 27.050659662004485 28.15103288650699 22.753931858799056 52-53 22.574567297338135 26.962871542082905 27.87319543128708 22.58936572929188 54-55 21.892028983245726 26.876858656467324 28.147413682795786 23.08369867749117 56-57 22.042478606988 27.21257197916906 27.92023675697997 22.82471265686297 58-59 22.310990328745582 26.669229520033838 27.824880657582913 23.19489949363767 60-61 22.00531864238653 26.812610977071632 28.25087933015859 22.931191050383244 62-63 22.0890697796878 26.787352134584612 28.12802792354418 22.995550162183406 64-65 21.751180331454243 26.731911186525764 28.000827131073486 23.516081350946504 66-67 21.477911566890757 27.02421874483224 28.035519649801298 23.4623500384757 68-69 21.462987708929955 26.734283946892084 28.390802310917174 23.41192603326079 70-71 21.324481086906353 26.9819521222055 28.121476858537992 23.572089932350156 72-73 21.640628762005797 26.799817089555173 27.89979771780991 23.65975643062912 74-75 21.763526016939213 27.07791124503719 27.446654542845117 23.711908195178477 76-77 21.848522499738472 26.910949891075536 27.607313997940803 23.63321361124519 78-79 21.937873638699198 27.036790763062378 27.386104300861007 23.639231297377414 80-81 21.79598864214311 27.176971497474277 27.380775895157793 23.64626396522482 82-83 21.92187376729457 27.161809845497864 27.172451246786938 23.743865140420628 84-85 21.831245749164417 27.0104445564444 27.07544897171748 24.0828607226737 86-87 21.900575192418835 26.903813516563158 27.312723036135296 23.882888254882715 88-89 21.863135625017087 26.926742848189388 27.083777107857898 24.126344418935627 90-91 21.742151367171733 26.976799398793773 27.239295050469913 24.041754183564585 92-93 21.943297820928088 26.77996558722801 27.23783012390873 24.03890646793517 94-95 21.56554885466118 26.50484144463897 27.378091717076387 24.55151798362346 96-97 21.86495654787661 26.41396942001077 27.31195060929129 24.40912342282133 98-99 21.708200596033294 26.508819384294668 26.99761072859932 24.785369291072712 100-101 21.47740092053606 26.49805463178191 27.380846655368536 24.643697792313503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 918.0 1 627.5 2 430.0 3 528.5 4 472.5 5 461.5 6 793.0 7 1670.5 8 2608.5 9 3003.5 10 2777.0 11 2512.5 12 2625.0 13 2780.0 14 2966.5 15 3166.5 16 3308.5 17 3466.5 18 3678.5 19 4031.5 20 4507.5 21 5132.5 22 5916.0 23 6877.5 24 8186.0 25 9883.0 26 12004.5 27 14797.5 28 17764.0 29 21605.5 30 26613.0 31 31656.5 32 37688.5 33 43266.5 34 48754.5 35 54567.0 36 61033.5 37 68283.5 38 73255.0 39 75729.0 40 79182.5 41 85458.0 42 91115.0 43 95792.0 44 100623.0 45 105026.5 46 106900.5 47 109367.0 48 116618.5 49 117703.0 50 112533.5 51 107411.5 52 100977.5 53 97389.0 54 94091.5 55 86213.0 56 76739.5 57 68405.5 58 61418.5 59 54469.0 60 45633.5 61 35945.0 62 27473.5 63 20263.5 64 15038.5 65 11724.0 66 8792.0 67 6565.5 68 4693.5 69 3149.0 70 2162.0 71 1575.0 72 1231.5 73 1040.0 74 835.5 75 651.0 76 462.5 77 271.0 78 156.0 79 83.5 80 49.5 81 31.5 82 22.5 83 15.0 84 6.5 85 4.5 86 4.5 87 3.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022236842926060294 2 0.025649427731544796 3 0.0 4 0.0 5 0.0 6 3.669446027402689E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.0363906301429584E-4 18-19 1.8347230137013446E-5 20-21 0.0 22-23 0.0 24-25 9.173615068506722E-5 26-27 2.5686122191818825E-4 28-29 7.338892054805378E-5 30-31 3.4859737260325546E-4 32-33 0.0022934037671266806 34-35 0.004201515701376079 36-37 0.10006579316727132 38-39 0.0919746646768484 40-41 0.10090976575357394 42-43 0.10197390510152073 44-45 0.09558906901384004 46-47 0.08988308044122888 48-49 0.09069035856725746 50-51 0.08986473321109185 52-53 0.07138907246311932 54-55 0.05994040085762292 56-57 0.032823194715117056 58-59 0.013925547673993205 60-61 0.009668990282206085 62-63 0.0018897647041123849 64-65 0.004586807534253361 66-67 0.1471080912385738 68-69 0.4021712846033347 70-71 0.3877320144855051 72-73 0.17429868630162773 74-75 0.030034415734291012 76-77 0.030896735550730643 78-79 0.00543078012055598 80-81 0.00165125071233121 82-83 7.338892054805378E-5 84-85 2.752084520552017E-4 86-87 0.0011742227287688605 88-89 2.0181953150714792E-4 90-91 6.971947452065109E-4 92-93 0.002054889775345506 94-95 0.07722349164668958 96-97 0.017760118772629015 98-99 0.01933798056441217 100-101 0.01001758765480934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2725207.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.259045242941816 #Duplication Level Percentage of deduplicated Percentage of total 1 72.00880685630493 32.590498473997634 2 14.00116408322283 12.67358637392868 3 5.426667216381835 7.368173311938437 4 2.6667471409791847 4.8277771802025065 5 1.5479913104434666 3.5030304377520824 6 0.9597148012749527 2.6061465366734398 7 0.606248212108668 1.9206750682195153 8 0.4279168040123084 1.5493684794406504 9 0.33215385033126554 1.3529669525784063 >10 1.7624641880600724 14.690269666509723 >50 0.15684543445243115 4.897237825179057 >100 0.0946932200026383 7.992150572286934 >500 0.006561674306283395 2.048944970679821 >1k 0.0017821831449117292 1.0747566049993298 >5k 1.6201664953742996E-4 0.5206963670669469 >10k+ 8.100832476871498E-5 0.3837211785468713 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10466 0.38404422122796544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8499 0.3118662178689545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 0.20926850694277535 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.669446027402689E-5 0.0 5 0.0 0.0 0.0 3.669446027402689E-5 0.0 6 0.0 0.0 0.0 3.669446027402689E-5 0.0 7 0.0 0.0 0.0 3.669446027402689E-5 0.0 8 0.0 0.0 0.0 3.669446027402689E-5 0.0 9 0.0 0.0 0.0 1.1008338082208067E-4 0.0 10-11 0.0 0.0 0.0 1.4677784109610756E-4 0.0 12-13 0.0 0.0 0.0 3.4859737260325546E-4 0.0 14-15 0.0 0.0 0.0 3.669446027402689E-4 0.0 16-17 0.0 0.0 0.0 4.5868075342533616E-4 0.0 18-19 0.0 0.0 0.0 6.788475150694975E-4 0.0 20-21 0.0 0.0 0.0 0.0010090976575357394 0.0 22-23 0.0 0.0 0.0 0.002018195315071479 0.0 24-25 0.0 0.0 0.0 0.003320848654799434 0.0 26-27 0.0 0.0 0.0 0.00543078012055598 0.0 28-29 0.0 0.0 0.0 0.012310991421936021 0.0 30-31 0.0 0.0 0.0 0.03366716730141967 0.0 32-33 0.0 0.0 0.0 0.08270931345765661 0.0 34-35 0.0 0.0 0.0 0.15303424657282916 0.0 36-37 0.0 0.0 0.0 0.2693923801017684 0.0 38-39 0.0 0.0 0.0 0.457102891633553 0.0 40-41 0.0 0.0 0.0 0.6647018006338601 0.0 42-43 0.0 0.0 0.0 0.8693468055821081 0.0 44-45 0.0 0.0 0.0 1.1073470749194465 0.0 46-47 0.0 0.0 0.0 1.390738391615756 0.0 48-49 0.0 0.0 0.0 1.6930457025833268 0.0 50-51 0.0 0.0 0.0 2.022561955844088 0.0 52-53 0.0 0.0 0.0 2.326557211984264 0.0 54-55 0.0 0.0 0.0 2.6498721014587145 0.0 56-57 0.0 0.0 0.0 3.015770912081174 0.0 58-59 0.0 0.0 0.0 3.401117786648867 0.0 60-61 0.0 0.0 0.0 3.807398850802893 0.0 62-63 0.0 0.0 0.0 4.194213503781548 0.0 64-65 0.0 0.0 0.0 4.579725503420475 0.0 66-67 0.0 0.0 0.0 4.992795042725195 0.0 68-69 0.0 0.0 0.0 5.454704908654645 0.0 70-71 0.0 0.0 0.0 5.981435538658165 0.0 72-73 0.0 0.0 0.0 6.521981632954855 0.0 74-75 0.0 0.0 0.0 7.056069502243316 0.0 76-77 0.0 0.0 0.0 7.618357064252367 0.0 78-79 0.0 0.0 0.0 8.23819988720123 0.0 80-81 0.0 0.0 0.0 8.904296077325503 0.0 82-83 0.0 0.0 0.0 9.579327368526501 0.0 84-85 0.0 0.0 0.0 10.228195509552119 0.0 86-87 0.0 0.0 0.0 10.873522635161294 0.0 88-89 0.0 0.0 0.0 11.553012303285584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9450 0.0 35.50252 1 GTATCAA 14465 0.0 33.3965 1 TATCAAC 17930 0.0 26.831785 2 ATCAACG 18085 0.0 26.492022 3 TCAACGC 18225 0.0 26.288515 4 CAACGCA 18440 0.0 25.95628 5 GTACATG 15905 0.0 25.658804 1 AACGCAG 18940 0.0 25.32115 6 TACATGG 15855 0.0 25.166431 2 ACATGGG 16220 0.0 23.718225 3 GAGTACT 9540 0.0 22.549717 12-13 ACGCAGA 21830 0.0 21.751268 7 CGCAGAG 21780 0.0 21.714083 8 GACGCGT 155 3.95007E-5 21.422522 8 AGTACTT 9850 0.0 21.286205 12-13 GTACTTT 10200 0.0 21.090618 14-15 TAAGGTG 1145 0.0 20.714607 5 CATGGGA 10395 0.0 20.580894 4 GTAAGGT 990 0.0 20.124556 4 GCAGAGT 23140 0.0 20.048399 9 >>END_MODULE