FastQCFastQC Report
Fri 27 May 2016
SRR939829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089948
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78280.2533375966197489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59290.19188025170650122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38480.12453284003484848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50200.051.889281
GTATCAA89300.041.708751
TATCAAC114750.033.037192
ATCAACG115750.032.713433
TCAACGC119050.031.687574
CAACGCA119650.031.4890145
AACGCAG125400.030.3478626
TACATGG117000.026.1566932
ACGCAGA144600.026.1546127
GTACATG116000.026.0958631
CGCAGAG146450.025.6601318
ACATGGG116150.025.1655063
TGGTATC26000.022.811072
CATGGGG63250.022.281644
GCAGAGT158150.022.231679
TAAGGTG15000.021.511415
GGACCGT3351.8189894E-1221.2470056
GAGTACT89850.021.04236812-13
AGAGTAC140300.019.95148510-11
CAGAGTA149400.019.75243610-11
Produced by FastQC (version 0.11.5)