FastQCFastQC Report
Fri 27 May 2016
SRR939829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089948
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88960.2879012850701695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72270.23388743111534563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.161232486760295No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.055.080411
GTATCAA91000.042.7125741
ATCAACG120400.032.0749173
TATCAAC122500.031.56562
TCAACGC123800.031.1544254
CAACGCA125600.030.6704045
AACGCAG130100.029.5913456
ACGCAGA150000.025.4770747
GTACATG122800.025.3521941
CGCAGAG150850.025.3022798
TACATGG121050.025.165412
ACATGGG119500.024.1880443
TGGTATC26450.022.9985452
GCAGAGT169000.022.0272549
GTGGTAT30500.021.9613191
TAAGGTG15900.020.7581185
AGAGTAC152200.020.71244610-11
CATGGGG63550.020.4034794
GAGTACT98400.020.06547212-13
CAGAGTA161050.019.17926210-11
Produced by FastQC (version 0.11.5)