##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939829_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3089948 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42243461702268 31.0 31.0 34.0 30.0 34.0 2 31.635233343732644 31.0 31.0 34.0 30.0 34.0 3 31.695034997352707 31.0 31.0 34.0 30.0 34.0 4 35.50513924506173 37.0 35.0 37.0 33.0 37.0 5 35.23788814569048 37.0 35.0 37.0 33.0 37.0 6 35.25676548602112 37.0 35.0 37.0 32.0 37.0 7 35.179146380456885 37.0 35.0 37.0 32.0 37.0 8 35.11048600170618 37.0 35.0 37.0 32.0 37.0 9 36.71501267982503 39.0 35.0 39.0 32.0 39.0 10-11 36.572856889501054 38.5 35.0 39.0 32.0 39.0 12-13 36.5174067654213 38.0 35.0 39.0 32.0 39.0 14-15 37.564563384238184 39.0 36.0 41.0 32.0 41.0 16-17 37.491781900536836 39.0 36.0 41.0 32.0 41.0 18-19 37.4666534841363 39.0 36.0 41.0 32.0 41.0 20-21 37.38804795420505 39.0 36.0 41.0 32.0 41.0 22-23 37.25233693253091 39.0 36.0 40.0 31.0 41.0 24-25 37.096286571812854 39.0 36.0 40.0 31.0 41.0 26-27 36.803967251228826 39.0 36.0 40.0 30.5 41.0 28-29 36.579309587086904 38.5 35.0 40.0 30.0 41.0 30-31 36.32257177143434 38.0 35.0 40.0 30.0 41.0 32-33 36.27511401486368 38.0 35.0 40.0 30.0 41.0 34-35 36.35209718739603 38.0 35.0 40.0 30.0 41.0 36-37 36.321959139765454 38.0 35.0 40.0 30.0 41.0 38-39 36.18467074526821 38.0 35.0 40.0 30.0 41.0 40-41 36.01038642074236 38.0 35.0 40.0 29.0 41.0 42-43 35.81722880126138 38.0 35.0 40.0 29.0 41.0 44-45 35.57456387615585 38.0 34.0 40.0 27.5 41.0 46-47 35.29371562239882 38.0 34.0 40.0 27.0 41.0 48-49 34.98525250263111 38.0 34.0 40.0 26.0 41.0 50-51 34.72238804665969 38.0 33.0 40.0 26.0 41.0 52-53 34.435074311930165 38.0 33.0 40.0 24.0 41.0 54-55 34.12285400919369 37.5 33.0 40.0 24.0 41.0 56-57 32.72295520830771 36.5 31.5 39.5 16.0 41.0 58-59 29.186121740560033 34.5 23.5 38.5 7.0 40.0 60-61 29.183644352591045 34.0 24.0 38.0 8.5 40.0 62-63 31.20252897459763 35.0 29.0 38.0 8.5 40.0 64-65 30.786474238401425 35.0 29.0 38.0 4.5 40.0 66-67 25.247475038414883 28.5 18.0 36.0 2.0 39.0 68-69 17.509663269414244 13.0 7.0 29.0 2.0 35.5 70-71 16.360611893792388 13.0 7.5 25.5 2.0 33.5 72-73 18.45602142819232 17.5 7.0 30.0 2.0 34.5 74-75 19.17009655178663 17.5 7.5 30.5 2.0 35.0 76-77 20.15416262668498 23.0 8.5 31.0 2.0 34.5 78-79 23.222850675804253 28.0 12.0 34.0 2.0 36.0 80-81 25.92192862145253 31.5 22.0 34.5 2.0 36.0 82-83 26.995463839520923 33.0 26.0 35.0 2.0 36.0 84-85 27.015188928745726 33.0 26.0 35.0 2.0 36.0 86-87 26.730353876505365 33.0 25.5 35.0 2.0 36.0 88-89 26.28877540981272 33.0 24.5 35.0 2.0 35.0 90-91 24.57250947265132 31.5 11.5 34.5 2.0 35.0 92-93 22.344663081708816 30.5 2.0 34.0 2.0 35.0 94-95 17.88786914860703 21.0 2.0 33.0 2.0 35.0 96-97 16.54417404435285 13.5 2.0 32.5 2.0 34.5 98-99 15.428250896131585 2.0 2.0 32.0 2.0 34.0 100-101 14.805002543732126 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48389089196427904 1101 2 0.5052569324903757 1101 3 0.467694353830975 1101 4 0.3576124238825571 1101 5 0.41301961992670044 1101 6 0.4244179526929486 1101 7 0.42074452024786524 1101 8 0.39989169391648005 1101 9 0.5609010901909457 1101 10-11 0.4944742876581145 1101 12-13 0.5333273360983313 1101 14-15 0.5448114205720955 1101 16-17 0.5504892399886856 1101 18-19 0.6718456889035664 1101 20-21 0.6463405112691305 1101 22-23 0.6552233243196994 1101 24-25 0.5564039227915671 1101 26-27 0.614303922159479 1101 28-29 0.7292905648962531 1101 30-31 0.7862998687537441 1101 32-33 0.43904592425818834 1101 34-35 0.6888517297637549 1101 36-37 0.644839082713645 1101 38-39 0.5103517356503531 1101 40-41 0.5073408501068783 1101 42-43 0.6142847974971488 1101 44-45 0.47038169752144654 1101 46-47 0.29596505709232446 1101 48-49 0.3668693349695147 1101 50-51 0.15414905113777166 1101 52-53 0.4374137933497195 1101 54-55 0.3636267729805738 1101 56-57 1.0697824811182528 1101 58-59 -8.176329700326775 1101 60-61 -9.281297880263406 1101 62-63 -2.3343054573087763 1101 64-65 0.1232212244558255 1101 66-67 -1.3288916597480807 1101 68-69 -3.710456850226988 1101 70-71 -4.049330587426589 1101 72-73 -6.5989398765261384 1101 74-75 -7.363081869539684 1101 76-77 -8.504145135779105 1101 78-79 -11.741380114263581 1101 80-81 -7.658951336665659 1101 82-83 -3.7560894704371535 1101 84-85 -2.675085763289392 1101 86-87 -2.536634702905374 1101 88-89 -2.812672765501649 1101 90-91 -8.580488353569628 1101 92-93 -14.527940459391303 1101 94-95 -12.701055747881396 1101 96-97 -12.639844115837569 1101 98-99 -12.141094365148586 1101 100-101 -11.822459075558431 1102 1 0.42091596226338623 1102 2 0.4873933954954879 1102 3 0.4268989358382278 1102 4 0.39481037710314126 1102 5 0.3931389031352097 1102 6 0.40886868397296183 1102 7 0.41859845647906013 1102 8 0.49719217542918415 1102 9 0.7349805573540209 1102 10-11 0.6958584068590525 1102 12-13 0.5567057600111909 1102 14-15 0.7280274506799671 1102 16-17 0.816349750758782 1102 18-19 0.8269722036315628 1102 20-21 0.9223684747221483 1102 22-23 0.7703249032757924 1102 24-25 0.6301041652499961 1102 26-27 0.8121691266362632 1102 28-29 0.7717483676830241 1102 30-31 0.623212584268046 1102 32-33 0.5264962479517408 1102 34-35 0.5859066581830916 1102 36-37 0.7132855739294257 1102 38-39 0.5863678847392535 1102 40-41 0.6632079925762824 1102 42-43 0.8851759700919004 1102 44-45 0.6371442686693172 1102 46-47 0.683468463506621 1102 48-49 0.7460103014500348 1102 50-51 0.7614567905656955 1102 52-53 0.6818621799608522 1102 54-55 0.6109786897564717 1102 56-57 1.3738101234535804 1102 58-59 -8.760558834912771 1102 60-61 -9.685407931884349 1102 62-63 -2.6906040152099884 1102 64-65 0.17497742331068622 1102 66-67 -1.2710559784388202 1102 68-69 -3.8530325600035837 1102 70-71 -4.231826742297066 1102 72-73 -6.799291467633365 1102 74-75 -7.889122803307703 1102 76-77 -8.70915311548097 1102 78-79 -11.925364897216966 1102 80-81 -7.634590445524012 1102 82-83 -3.405850188604859 1102 84-85 -2.4967673241252655 1102 86-87 -2.319205712421855 1102 88-89 -2.4552452895868413 1102 90-91 -8.075807298621545 1102 92-93 -14.241742039603462 1102 94-95 -12.430890275788325 1102 96-97 -12.472637823387092 1102 98-99 -11.955366826958063 1102 100-101 -11.639621141510254 1103 1 0.3371407619770572 1103 2 0.34704140846428544 1103 3 0.24940926335765923 1103 4 0.20075044016545007 1103 5 0.23139834539841786 1103 6 0.24880402942755353 1103 7 0.2839476074878391 1103 8 0.35335926687901065 1103 9 0.533293919000215 1103 10-11 0.3642023862709891 1103 12-13 0.3735980567879196 1103 14-15 0.4336101523640181 1103 16-17 0.4515477321874073 1103 18-19 0.49602530141378764 1103 20-21 0.5071242734576984 1103 22-23 0.5180925645913348 1103 24-25 0.4918870885384621 1103 26-27 0.37124730771184744 1103 28-29 0.44138575374314115 1103 30-31 0.4059414670646575 1103 32-33 0.41653745654158314 1103 34-35 0.4120019886806219 1103 36-37 0.15783141228358488 1103 38-39 0.28433939009995157 1103 40-41 0.3193825602999354 1103 42-43 0.25457037161449136 1103 44-45 0.23282219304820728 1103 46-47 0.1488940236125984 1103 48-49 0.17208793922716126 1103 50-51 0.13442745583228088 1103 52-53 0.16889231081030687 1103 54-55 0.1325800467170879 1103 56-57 0.741022535556624 1103 58-59 -6.4914376725923795 1103 60-61 -8.542117363288352 1103 62-63 -2.5487741954998278 1103 64-65 -0.32218706170485234 1103 66-67 -1.8078980522466743 1103 68-69 -4.06185351440914 1103 70-71 -4.846112807763063 1103 72-73 -7.779420704555015 1103 74-75 -8.214858866710113 1103 76-77 -9.19908382632238 1103 78-79 -12.151331326830498 1103 80-81 -8.44811497976794 1103 82-83 -4.504107145542648 1103 84-85 -3.646902291729205 1103 86-87 -3.33981542003076 1103 88-89 -3.5366858175764087 1103 90-91 -9.21967538963678 1103 92-93 -14.853176070113426 1103 94-95 -12.7807895321528 1103 96-97 -12.762288292362587 1103 98-99 -12.230554934743603 1103 100-101 -11.83752504466646 1104 1 0.15406627889367996 1104 2 0.20414814621001653 1104 3 0.19289889880095856 1104 4 0.15524171006237708 1104 5 0.04754426157323621 1104 6 0.12415969554294293 1104 7 0.19456374576478197 1104 8 0.2720823977327882 1104 9 0.3099934628696488 1104 10-11 0.19305151884758942 1104 12-13 0.2361805277146516 1104 14-15 0.31065200794490266 1104 16-17 0.3381926441452592 1104 18-19 0.22454152879610234 1104 20-21 0.26996488201945823 1104 22-23 0.2989115928924164 1104 24-25 0.29971008981033975 1104 26-27 0.30233359511913704 1104 28-29 0.2650789747227549 1104 30-31 0.14072271993293128 1104 32-33 0.22021474915211314 1104 34-35 0.20173183920822169 1104 36-37 0.12601700343758182 1104 38-39 0.2027372834961838 1104 40-41 0.251447695585604 1104 42-43 0.12993330640223633 1104 44-45 -5.381056343622959E-4 1104 46-47 -0.02908754303428651 1104 48-49 -0.018157302927939156 1104 50-51 -0.05666337520785447 1104 52-53 0.006737209313989467 1104 54-55 -0.14821874331975238 1104 56-57 0.6134360996403672 1104 58-59 -7.066423006976962 1104 60-61 -9.19903046930322 1104 62-63 -3.0650632902679185 1104 64-65 -0.792093760456293 1104 66-67 -2.140826477772425 1104 68-69 -4.214862345703539 1104 70-71 -4.836471982794404 1104 72-73 -7.803132824082773 1104 74-75 -8.525435839670083 1104 76-77 -9.148981580331125 1104 78-79 -12.144265179631617 1104 80-81 -8.412600674165589 1104 82-83 -4.682496612979854 1104 84-85 -3.625033849568169 1104 86-87 -3.407550610039557 1104 88-89 -3.6066349596735385 1104 90-91 -9.140151553237207 1104 92-93 -15.020621767429946 1104 94-95 -12.794187690641783 1104 96-97 -12.559165100622991 1104 98-99 -11.755973624524078 1104 100-101 -11.226108696823715 1105 1 0.15916584655255406 1105 2 0.19115628341073432 1105 3 0.12129966730106645 1105 4 0.15429778520886117 1105 5 0.216088776918113 1105 6 0.13232177920226462 1105 7 0.1413237234658382 1105 8 0.16162644173731167 1105 9 0.2612941947631242 1105 10-11 0.16363485636794906 1105 12-13 0.2016161586527616 1105 14-15 0.25952521738989276 1105 16-17 0.3239943893008572 1105 18-19 0.3235028095020098 1105 20-21 0.30594844013668165 1105 22-23 0.24785791990691308 1105 24-25 0.1923864656097649 1105 26-27 0.2981045954852064 1105 28-29 0.1631711708099104 1105 30-31 0.2503956288685387 1105 32-33 0.23566117067394288 1105 34-35 0.3644581005221781 1105 36-37 0.18898754825439568 1105 38-39 0.30217770440072655 1105 40-41 0.25475363375627325 1105 42-43 0.3010733040575033 1105 44-45 0.21247926218098456 1105 46-47 0.3515134757308047 1105 48-49 0.35652865758952856 1105 50-51 0.35017460094758945 1105 52-53 0.24393424154457222 1105 54-55 0.161109393306738 1105 56-57 0.7436638024901896 1105 58-59 -6.465885678974324 1105 60-61 -8.683168167678893 1105 62-63 -2.5149465079195004 1105 64-65 -0.1978871793965169 1105 66-67 -1.4340436747568042 1105 68-69 -3.9615141783083274 1105 70-71 -4.681429861236445 1105 72-73 -6.07995972254362 1105 74-75 -6.673993562115214 1105 76-77 -8.617034514006118 1105 78-79 -11.879470715129287 1105 80-81 -7.793270121022562 1105 82-83 -3.793375339738823 1105 84-85 -2.841051095361397 1105 86-87 -2.604764773219024 1105 88-89 -2.6855786787642586 1105 90-91 -8.434342726013352 1105 92-93 -11.68943052048349 1105 94-95 -11.80657685986254 1105 96-97 -12.26604920592569 1105 98-99 -11.699693815835625 1105 100-101 -11.2695864708531 1106 1 0.15225513854920436 1106 2 0.14847015823134768 1106 3 0.07010059466827201 1106 4 0.08710901890589895 1106 5 0.15492516393193512 1106 6 0.14756006245929143 1106 7 0.1229860494262951 1106 8 0.12820588931600696 1106 9 0.08463175490658159 1106 10-11 0.11748644218105397 1106 12-13 0.15366039082865512 1106 14-15 0.2807498786756 1106 16-17 0.05109041888346866 1106 18-19 0.0805706687871961 1106 20-21 0.0959843553676194 1106 22-23 0.26913634014295695 1106 24-25 0.17069776637894307 1106 26-27 0.18981053098965361 1106 28-29 0.25717512426944467 1106 30-31 0.23549230133124865 1106 32-33 0.355861425294961 1106 34-35 0.3304077787024724 1106 36-37 0.3266168392892581 1106 38-39 0.39218795504803694 1106 40-41 0.5110918494349548 1106 42-43 0.42770458700827163 1106 44-45 0.3380696868108828 1106 46-47 0.3394808356449488 1106 48-49 0.3457932685584737 1106 50-51 0.4089478136016922 1106 52-53 0.386324945599533 1106 54-55 0.16340361472578735 1106 56-57 0.9595470412897669 1106 58-59 -6.564507991975937 1106 60-61 -8.944263443016343 1106 62-63 -2.547285047569254 1106 64-65 -0.11153572646217569 1106 66-67 -1.4816793692824533 1106 68-69 -4.167897522145411 1106 70-71 -4.711435758522427 1106 72-73 -5.956777212932057 1106 74-75 -6.547423276843496 1106 76-77 -8.441742117455398 1106 78-79 -11.86025062487297 1106 80-81 -7.847000825128134 1106 82-83 -3.7291586030893455 1106 84-85 -2.7922875765293114 1106 86-87 -2.4286046403908834 1106 88-89 -2.5929857711997855 1106 90-91 -8.215685130536801 1106 92-93 -11.479151019500186 1106 94-95 -11.762615655398827 1106 96-97 -12.223454956918484 1106 98-99 -11.620669778700439 1106 100-101 -11.380027349928366 1107 1 0.029927432906582396 1107 2 0.1001550660874031 1107 3 0.06251763788117515 1107 4 -0.039803399503149706 1107 5 -0.021103761810941535 1107 6 0.07033985732505244 1107 7 0.1037378573614447 1107 8 -0.007021084656699372 1107 9 -0.09625383225199613 1107 10-11 -0.013040830274604787 1107 12-13 0.05519521372108471 1107 14-15 0.018399106177824365 1107 16-17 0.12678223399131383 1107 18-19 0.06830035792733469 1107 20-21 0.04185788522145373 1107 22-23 0.004548393667249684 1107 24-25 0.004986832549356279 1107 26-27 0.19017287512603787 1107 28-29 0.12389554042811568 1107 30-31 0.07282305623367336 1107 32-33 0.007571322752234266 1107 34-35 0.015772286944418568 1107 36-37 -0.04794151092146137 1107 38-39 -0.21211715670243336 1107 40-41 -0.275105191588942 1107 42-43 -0.0194521116123596 1107 44-45 -0.08221455237261921 1107 46-47 -0.08618018198973942 1107 48-49 -0.08036261228533448 1107 50-51 -0.0041462496353119604 1107 52-53 -0.06357776365332057 1107 54-55 -0.08935669963303638 1107 56-57 0.46201541226959364 1107 58-59 -6.94675712315793 1107 60-61 -9.165222431920206 1107 62-63 -2.841580944975913 1107 64-65 -0.5963238275502434 1107 66-67 -2.0633233130947737 1107 68-69 -4.258190113892258 1107 70-71 -5.0887774157608305 1107 72-73 -6.413510497871538 1107 74-75 -6.973416964098281 1107 76-77 -9.492459270230276 1107 78-79 -12.567378138810085 1107 80-81 -8.755024560269664 1107 82-83 -4.836227560669315 1107 84-85 -3.9558726666295776 1107 86-87 -3.739731527098151 1107 88-89 -3.699515811108963 1107 90-91 -8.993567920872216 1107 92-93 -11.802801161831159 1107 94-95 -12.036169218358038 1107 96-97 -12.554580028602256 1107 98-99 -11.885602538990408 1107 100-101 -11.412197218648103 1108 1 -0.001312240718775115 1108 2 0.026962913336738836 1108 3 0.010763574701314838 1108 4 -0.03602925096795673 1108 5 -0.09187145206652048 1108 6 -0.06936974632601078 1108 7 -0.037161020776927955 1108 8 -0.11309922508672798 1108 9 -0.034248765952206384 1108 10-11 0.045045631361844585 1108 12-13 -0.07947652413519535 1108 14-15 0.013421775740404485 1108 16-17 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2311 62-63 1.5612984130972514 2311 64-65 1.60358436556362 2311 66-67 -0.10414075134613654 2311 68-69 -3.161008629917049 2311 70-71 -0.7633785238576749 2311 72-73 -0.9286618411886707 2311 74-75 1.466632376990237 2311 76-77 4.5465174287990315 2311 78-79 5.392198571578884 2311 80-81 3.2370302769613595 2311 82-83 1.8668078393631973 2311 84-85 1.4032844520806478 2311 86-87 1.4655421073418111 2311 88-89 1.5680426044618407 2311 90-91 3.0299182717941484 2311 92-93 5.0603646995421165 2311 94-95 8.883645191548926 2311 96-97 9.648505244247605 2311 98-99 10.234311374856324 2311 100-101 9.531099154264107 2312 1 -0.3236057885125696 2312 2 -0.2615223371005122 2312 3 -0.28275213628508666 2312 4 -0.26756495422797144 2312 5 -0.30908576106956076 2312 6 -0.21163481554577146 2312 7 -0.2415627997023364 2312 8 -0.2671132306906969 2312 9 -0.38302203162233894 2312 10-11 -0.42962083779963933 2312 12-13 -0.38373900357414215 2312 14-15 -0.5250752958416385 2312 16-17 -0.5263283090682123 2312 18-19 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2312 84-85 1.423182487350413 2312 86-87 1.1592560332150796 2312 88-89 1.3800186862468387 2312 90-91 2.833967975395378 2312 92-93 4.777504387806978 2312 94-95 8.933203804104771 2312 96-97 9.532908947919044 2312 98-99 10.09278819680807 2312 100-101 9.295393584258077 2313 1 -0.36981420441046353 2313 2 -0.3391547746457846 2313 3 -0.3151549595440457 2313 4 -0.3434373448386623 2313 5 -0.40093746214893855 2313 6 -0.3476235847700906 2313 7 -0.46843225091491547 2313 8 -0.4187867719171052 2313 9 -0.5301362131705787 2313 10-11 -0.6448096237863155 2313 12-13 -0.4492582473526099 2313 14-15 -0.6198508299919396 2313 16-17 -0.5277932188807739 2313 18-19 -0.6140004617727541 2313 20-21 -0.5810729781044088 2313 22-23 -0.5753940715533332 2313 24-25 -0.5931216724036048 2313 26-27 -0.6152348595318955 2313 28-29 -0.7105934351449505 2313 30-31 -0.8204298801877243 2313 32-33 -0.6925388247347968 2313 34-35 -0.6909409949990462 2313 36-37 -0.8438431948414973 2313 38-39 -0.9641674208259658 2313 40-41 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2314 4 -0.31731620231156654 2314 5 -0.4469890532705634 2314 6 -0.3147695705228841 2314 7 -0.39064245912219064 2314 8 -0.4673591287574155 2314 9 -0.460217281986246 2314 10-11 -0.4140216954074205 2314 12-13 -0.5128322888290384 2314 14-15 -0.637463528710235 2314 16-17 -0.6554497868988207 2314 18-19 -0.658106813882334 2314 20-21 -0.5795149475471746 2314 22-23 -0.622115477433745 2314 24-25 -0.7054325805943691 2314 26-27 -0.7672402641196072 2314 28-29 -0.6608718298586354 2314 30-31 -0.7041134058217011 2314 32-33 -0.7796627039360757 2314 34-35 -0.7958251504806384 2314 36-37 -0.8227254215866395 2314 38-39 -0.6162067759885232 2314 40-41 -0.6975795076788742 2314 42-43 -0.886528649589799 2314 44-45 -0.8842854737423522 2314 46-47 -0.7916239666997384 2314 48-49 -0.8910883383991788 2314 50-51 -0.7691366083823752 2314 52-53 -0.8569815770559259 2314 54-55 -0.6648865118336573 2314 56-57 0.5489479820326011 2314 58-59 3.817912326522631 2314 60-61 3.535485217776465 2314 62-63 1.0637903532452633 2314 64-65 1.0015861065193938 2314 66-67 1.7523703053353081 2314 68-69 -0.17020967303422196 2314 70-71 2.4379576276210813 2314 72-73 8.186785049778472 2314 74-75 9.783468599896374 2314 76-77 8.640413534203656 2314 78-79 6.133334077992092 2314 80-81 3.097289927632911 2314 82-83 1.562093944171444 2314 84-85 1.1814208163159172 2314 86-87 1.1731505625408936 2314 88-89 1.2074058796223106 2314 90-91 2.585285718868537 2314 92-93 4.467404691594954 2314 94-95 8.515075476343075 2314 96-97 9.158459178786835 2314 98-99 9.339451138480227 2314 100-101 8.506599692049887 2315 1 -0.7429587427913091 2315 2 -0.5485645425672665 2315 3 -0.6212469538948007 2315 4 -0.4295379234766088 2315 5 -0.790789659245597 2315 6 -0.7619504395193246 2315 7 -0.8391149510828626 2315 8 -0.8378955376853909 2315 9 -1.1530685486844163 2315 10-11 -0.9465337832118408 2315 12-13 -0.7506385900473305 2315 14-15 -0.8607021526311271 2315 16-17 -1.0100917775397704 2315 18-19 -0.9716425254923919 2315 20-21 -0.9847122172017393 2315 22-23 -1.0207427948632386 2315 24-25 -1.0044609458736957 2315 26-27 -0.9686329549599577 2315 28-29 -0.9278450918945751 2315 30-31 -1.0944541299085557 2315 32-33 -1.0395533806225998 2315 34-35 -1.0115745403274587 2315 36-37 -1.1695632865259569 2315 38-39 -1.1003434236470255 2315 40-41 -0.9490769762995726 2315 42-43 -0.883362984717337 2315 44-45 -0.7603486494491776 2315 46-47 -0.8728660066139895 2315 48-49 -0.795443405768161 2315 50-51 -0.6869622299174054 2315 52-53 -0.7049970664463245 2315 54-55 -1.0217403077500293 2315 56-57 0.2567347648324727 2315 58-59 3.6905270021833587 2315 60-61 3.395966075391062 2315 62-63 0.8254838452808535 2315 64-65 0.8171112092441177 2315 66-67 1.5876483968741333 2315 68-69 5.641894303694951 2315 70-71 4.4995819281420495 2315 72-73 8.623590703384352 2315 74-75 9.7018857350435 2315 76-77 8.424730958651278 2315 78-79 5.84812465569982 2315 80-81 2.79622030461681 2315 82-83 1.202911462987501 2315 84-85 0.7719193373968203 2315 86-87 0.7854837609249614 2315 88-89 0.9455105039638916 2315 90-91 2.1053920490456903 2315 92-93 4.0844709893842825 2315 94-95 8.162697787923161 2315 96-97 8.839674583029524 2315 98-99 8.950883964721283 2315 100-101 8.356831308934199 2316 1 -0.4491512183193933 2316 2 -0.43235881428449474 2316 3 -0.5654501233606339 2316 4 -0.3921599694780227 2316 5 -0.6503654063488185 2316 6 -0.5784684939872307 2316 7 -0.5675893341763967 2316 8 -0.6864165890468783 2316 9 -0.7302946286229215 2316 10-11 -0.6698870221989424 2316 12-13 -0.6263184747096133 2316 14-15 -0.6888861040113596 2316 16-17 -0.7817155872677333 2316 18-19 -0.7385037493624722 2316 20-21 -0.6819966661733972 2316 22-23 -0.807925439689221 2316 24-25 -2.12589008295069 2316 26-27 -1.4536027604333412 2316 28-29 -0.9941958722232798 2316 30-31 -0.6939229900537072 2316 32-33 -0.6091278621710572 2316 34-35 -0.7548440729764323 2316 36-37 -0.6839366000946683 2316 38-39 -1.0490612163547794 2316 40-41 -0.6904137765540312 2316 42-43 -0.8468905763385379 2316 44-45 -0.826895160178033 2316 46-47 -0.8422396281468423 2316 48-49 -0.9504980146434079 2316 50-51 -1.0554060805662218 2316 52-53 -0.8261413132924034 2316 54-55 -0.7862362453379532 2316 56-57 0.3702635452514187 2316 58-59 3.9804234756850576 2316 60-61 3.794227688026087 2316 62-63 1.265744465316569 2316 64-65 0.911328531955526 2316 66-67 6.1272550190204775 2316 68-69 8.9642690123693 2316 70-71 5.860472981662099 2316 72-73 9.349191799774133 2316 74-75 10.213500242195103 2316 76-77 8.761482912351443 2316 78-79 6.234966508719111 2316 80-81 3.2458701802797982 2316 82-83 1.7804232756120086 2316 84-85 1.440639971986414 2316 86-87 1.3105786260488372 2316 88-89 1.5049238538068472 2316 90-91 2.9073412467569817 2316 92-93 4.892550217979981 2316 94-95 9.002230358266761 2316 96-97 9.780299330878034 2316 98-99 10.249192118319941 2316 100-101 9.137933346907955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 30.0 4 49.0 5 137.0 6 302.0 7 733.0 8 1852.0 9 3117.0 10 5240.0 11 7841.0 12 11189.0 13 15033.0 14 19885.0 15 27604.0 16 36360.0 17 46753.0 18 51537.0 19 53598.0 20 55481.0 21 55261.0 22 52965.0 23 51224.0 24 53406.0 25 57431.0 26 74974.0 27 109180.0 28 153872.0 29 202342.0 30 238467.0 31 253859.0 32 258000.0 33 266733.0 34 269068.0 35 259643.0 36 217277.0 37 134599.0 38 42857.0 39 2048.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.807872296829466 19.773708448772517 14.733260630520487 23.685158623877534 2 15.607874994336166 20.976056547715373 39.66408611616361 23.751982341784853 3 19.100240662514516 24.82908300979609 30.126603499768915 25.94407282792048 4 13.046433143858732 17.233817527026346 37.10635259881396 32.61339673030096 5 13.135718639024704 38.091822373461845 34.66214933992063 14.110309647592814 6 31.842745370142627 37.04080073943088 17.26371092809449 13.852742962332004 7 28.182060021722048 32.873401105779124 20.9862107711845 17.95832810131433 8 26.14747959996712 35.87774673084902 19.676104372050514 18.298669297133348 9 26.238499806469235 16.535553349117848 20.008621504310106 37.21732534010281 10-11 24.75734219475538 26.887653125554216 27.666290824311606 20.688713855378797 12-13 25.656143727985064 23.941406133695452 28.07238180059988 22.3300683377196 14-15 22.31258709710409 25.540851575854823 26.090829536606186 26.055731790434898 16-17 21.900692179933127 28.786164039006483 26.95932099828217 22.35382278277822 18-19 21.928180697965878 27.30633522200907 29.183284612888897 21.58219946713615 20-21 22.554004145053575 26.511271387091305 29.312629209294137 21.622095258560986 22-23 22.20809217501395 26.402272789056646 29.294198478420995 22.09543655750841 24-25 22.017344628453294 26.678167399580833 29.35745197006552 21.947036001900354 26-27 21.691272548334588 27.03500757537633 29.068862217796397 22.20485765849268 28-29 21.754584121862436 27.30925416946141 29.038610753986198 21.89755095468995 30-31 21.964927282604773 26.91253246911924 28.867889139491272 22.254651108784717 32-33 21.6559649973616 27.011508481479485 28.85248363357394 22.48004288758498 34-35 22.19588560898447 27.086852515631115 28.77007279013421 21.947189085250205 36-37 22.281240910682264 26.641993089002764 28.898159015089824 22.178606985225148 38-39 21.910085262356795 26.94960881090752 28.903239736485936 22.237066190249745 40-41 22.31454827235978 26.786042916857188 28.90629590214018 21.993112908642853 42-43 21.947876508495874 26.793580109592252 28.786110588662606 22.47243279324927 44-45 21.919548782487382 26.960702827668374 28.93971621505475 22.18003217478949 46-47 22.276746780376726 27.14977148136662 28.306997092287347 22.26648464596931 48-49 21.93193205669183 27.352508476403415 28.28592854716056 22.4296309197442 50-51 22.091399616822706 27.39814781994066 28.243568945126206 22.266883618110427 52-53 22.365450257907575 27.104766960639758 28.237100170427787 22.29268261102488 54-55 21.887122426054262 27.34462457571256 28.344739644942642 22.423513353290538 56-57 21.948754697766933 27.411866075944403 28.165372415772406 22.474006810516258 58-59 20.451432920750882 26.966401485159896 29.875540588322462 22.706625005766764 60-61 21.739347780289155 27.23323884025736 28.528290629669268 22.499122749784217 62-63 21.62509890984762 27.21406760983645 28.642544260639784 22.51828921967614 64-65 20.825667260291063 27.180257100401384 29.053025945005214 22.94104969430234 66-67 21.111778168751215 27.075360978178136 29.08444780179167 22.728413051278977 68-69 22.08187365038708 26.36152915330107 29.34557287074645 22.2110243255654 70-71 21.157493812969815 26.62793958336916 29.441996674677952 22.772569928983078 72-73 20.869942229394635 26.924427571362113 29.350044847734402 22.855585351508847 74-75 21.403389035188315 27.130107984093193 28.6980165127401 22.768486467978388 76-77 19.848987930551594 27.404630155560035 29.43802857823416 23.308353335654214 78-79 20.88950284860845 27.58014189098727 28.305406181798805 23.224949078605476 80-81 21.520410907522184 27.651353213540325 27.55046131948798 23.27777455944952 82-83 21.800116474583927 27.607372883234632 27.41321081980599 23.17929982237545 84-85 21.68415833669639 27.661218784658764 27.309546086065556 23.345076792579288 86-87 21.653999458876562 27.64192515667336 27.28778381471312 23.41629156973696 88-89 21.595568078605893 27.68876419057741 27.09517786149372 23.62048986932298 90-91 21.278254698683575 27.774094740406408 27.281895716274366 23.665754844635643 92-93 21.11569322277913 27.543415066808745 27.515632795528646 23.825258914883484 94-95 19.79182235087747 27.55785880785881 28.497404826144983 24.15291401511874 96-97 19.65529596743237 27.334191934143863 28.75637171964876 24.25414037877501 98-99 19.89873198456386 27.45616608150629 28.124188544474503 24.520913389455348 100-101 19.704372036292057 27.586598471904917 28.158850278295517 24.550179213507516 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 326.0 1 252.0 2 183.0 3 215.0 4 245.0 5 282.0 6 390.0 7 574.5 8 829.5 9 1078.5 10 1245.5 11 1412.0 12 1635.5 13 1925.5 14 2208.0 15 2492.5 16 2866.0 17 3180.5 18 3520.0 19 4025.0 20 4672.5 21 5578.0 22 6788.5 23 8411.5 24 10566.0 25 13260.5 26 16700.0 27 21094.5 28 26002.5 29 31042.0 30 36746.0 31 43960.5 32 52269.0 33 59445.5 34 65492.0 35 72499.0 36 80308.0 37 87199.0 38 93044.0 39 98258.5 40 103380.0 41 108203.5 42 112112.5 43 115858.5 44 119164.5 45 121096.0 46 121944.0 47 122651.5 48 122305.5 49 120644.0 50 118136.0 51 114623.5 52 109794.0 53 104547.0 54 98682.5 55 90646.5 56 81242.0 57 71203.5 58 61049.0 59 50804.0 60 40600.0 61 31722.0 62 24464.5 63 17965.5 64 12772.0 65 9306.5 66 6701.5 67 4771.5 68 3409.5 69 2411.5 70 1732.5 71 1225.0 72 861.0 73 619.0 74 445.0 75 304.0 76 191.5 77 116.5 78 73.0 79 45.0 80 26.5 81 15.5 82 9.0 83 5.5 84 3.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021327219746092814 2 0.00543698470006615 3 0.0046602726000567005 4 0.0 5 0.06427292627578199 6 1.2945201666824166E-4 7 0.0 8 6.472600833412083E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 1.941780250023625E-4 16-17 0.0 18-19 0.0013107016687659468 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.090751041765105E-5 28-29 0.002281591793777759 30-31 0.009110185673027507 32-33 3.0744853958707396E-4 34-35 0.0012136126562647656 36-37 0.02632730388990365 38-39 0.025146054237805943 40-41 0.02703928998157898 42-43 0.027864546587839017 44-45 0.028592714181597877 46-47 0.0298386898420297 48-49 0.03014613838161678 50-51 0.025162235739889474 52-53 0.021456671762761056 54-55 0.01629477259811492 56-57 0.14797983655388375 58-59 0.7379897655235622 60-61 0.3009435757494948 62-63 4.207190541717854E-4 64-65 0.1418470472642258 66-67 1.3776445428855113 68-69 2.635853418892486 70-71 2.1320747145259404 72-73 1.8056129099907183 74-75 1.5120642806933968 76-77 1.0885620081632443 78-79 0.40958618073831665 80-81 2.2654102916942292E-4 82-83 1.1327051458471146E-4 84-85 3.2363004167060416E-5 86-87 0.0025890403333648335 88-89 4.8544506250590624E-5 90-91 0.21825610010265548 92-93 0.169808682864566 94-95 1.2589208620986503 96-97 1.2324964691962452 98-99 0.9788352425348259 100-101 0.3946506543152183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3089948.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.53438139963229 #Duplication Level Percentage of deduplicated Percentage of total 1 70.75629517597318 34.34113016495644 2 15.035521430142069 14.594794632657196 3 5.8213248015166785 8.476031945038475 4 2.884310680001043 5.599529384728136 5 1.5645767504478554 3.796788236761676 6 0.9522778203844324 2.7730928959769146 7 0.627764452610833 2.1327711560501896 8 0.4062794203770945 1.5774816274722756 9 0.31160588542477885 1.3611239000618705 >10 1.46394526994597 12.77295992389173 >50 0.11090343627769884 3.7441873143833586 >100 0.059179526077487186 5.196579869957472 >500 0.0035423732613428962 1.1621609315772405 >1k 0.0023393030966583723 1.9483534266094453 >5k 1.336744626661927E-4 0.5230145898775279 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8896 0.2879012850701695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7227 0.23388743111534563 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4982 0.161232486760295 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.2363004167060416E-5 0.0 8 0.0 0.0 0.0 3.2363004167060416E-5 0.0 9 0.0 0.0 0.0 6.472600833412083E-5 0.0 10-11 0.0 0.0 0.0 6.472600833412083E-5 0.0 12-13 0.0 0.0 0.0 8.090751041765103E-5 0.0 14-15 0.0 0.0 0.0 9.708901250118125E-5 1.6181502083530208E-5 16-17 0.0 0.0 0.0 1.1327051458471146E-4 3.2363004167060416E-5 18-19 0.0 0.0 0.0 1.456335187517719E-4 4.8544506250590624E-5 20-21 0.0 0.0 0.0 3.2363004167060413E-4 6.472600833412083E-5 22-23 0.0 0.0 0.0 5.987155770906177E-4 6.472600833412083E-5 24-25 0.0 0.0 0.0 0.0010032531291788729 6.472600833412083E-5 26-27 0.0 0.0 0.0 0.002103595270858927 6.472600833412083E-5 28-29 0.0 0.0 0.0 0.007184586925087413 6.472600833412083E-5 30-31 0.0 0.0 0.0 0.024239890121128253 6.472600833412083E-5 32-33 0.0 0.0 0.0 0.062298783021591295 9.708901250118125E-5 34-35 0.0 0.0 0.0 0.12249397077232367 1.2945201666824166E-4 36-37 0.0 0.0 0.0 0.22623358062983584 1.2945201666824166E-4 38-39 0.0 0.0 0.0 0.3841974039692577 1.2945201666824166E-4 40-41 0.0 0.0 0.0 0.557339476263031 1.2945201666824166E-4 42-43 0.0 0.0 0.0 0.7292032098922053 1.456335187517719E-4 44-45 0.0 0.0 0.0 0.9369736966447332 1.618150208353021E-4 46-47 0.0 0.0 0.0 1.172786726508019 1.9417802500236252E-4 48-49 0.0 0.0 0.0 1.4006222758441242 2.589040333364833E-4 50-51 0.0 0.0 0.0 1.6340728064032146 2.589040333364833E-4 52-53 0.0 0.0 0.0 1.855257758382989 2.589040333364833E-4 54-55 0.0 0.0 0.0 2.098465734698448 2.589040333364833E-4 56-57 0.0 0.0 0.0 2.3090032583072597 2.589040333364833E-4 58-59 0.0 0.0 0.0 2.4512710246256573 2.589040333364833E-4 60-61 0.0 0.0 0.0 2.5590236469998846 2.7508553542001356E-4 62-63 3.2363004167060416E-5 0.0 0.0 2.651258208876007 2.9126703750354373E-4 64-65 3.2363004167060416E-5 0.0 0.0 2.746356896620914 3.236300416706042E-4 66-67 3.2363004167060416E-5 0.0 0.0 2.827571855578152 3.236300416706042E-4 68-69 3.2363004167060416E-5 0.0 3.2363004167060416E-5 2.9201462289980284 3.236300416706042E-4 70-71 3.2363004167060416E-5 0.0 3.2363004167060416E-5 3.092107051639704 3.236300416706042E-4 72-73 3.2363004167060416E-5 0.0 3.2363004167060416E-5 3.3057028791423027 3.236300416706042E-4 74-75 3.2363004167060416E-5 0.0 3.2363004167060416E-5 3.5554320007974245 3.236300416706042E-4 76-77 3.2363004167060416E-5 0.0 3.2363004167060416E-5 3.9051466238266794 3.236300416706042E-4 78-79 3.2363004167060416E-5 0.0 3.2363004167060416E-5 4.410721474924497 3.236300416706042E-4 80-81 3.2363004167060416E-5 0.0 3.2363004167060416E-5 4.969824734914633 3.236300416706042E-4 82-83 3.2363004167060416E-5 3.2363004167060416E-5 3.2363004167060416E-5 5.507843497689929 3.236300416706042E-4 84-85 3.2363004167060416E-5 3.2363004167060416E-5 3.2363004167060416E-5 5.945019139480664 3.236300416706042E-4 86-87 3.2363004167060416E-5 3.2363004167060416E-5 3.2363004167060416E-5 6.327679300751987 3.236300416706042E-4 88-89 3.2363004167060416E-5 3.2363004167060416E-5 3.2363004167060416E-5 6.714821738100447 3.236300416706042E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4890 0.0 55.08041 1 GTATCAA 9100 0.0 42.712574 1 ATCAACG 12040 0.0 32.074917 3 TATCAAC 12250 0.0 31.5656 2 TCAACGC 12380 0.0 31.154425 4 CAACGCA 12560 0.0 30.670404 5 AACGCAG 13010 0.0 29.591345 6 ACGCAGA 15000 0.0 25.477074 7 GTACATG 12280 0.0 25.352194 1 CGCAGAG 15085 0.0 25.302279 8 TACATGG 12105 0.0 25.16541 2 ACATGGG 11950 0.0 24.188044 3 TGGTATC 2645 0.0 22.998545 2 GCAGAGT 16900 0.0 22.027254 9 GTGGTAT 3050 0.0 21.961319 1 TAAGGTG 1590 0.0 20.758118 5 AGAGTAC 15220 0.0 20.712446 10-11 CATGGGG 6355 0.0 20.403479 4 GAGTACT 9840 0.0 20.065472 12-13 CAGAGTA 16105 0.0 19.179262 10-11 >>END_MODULE