FastQCFastQC Report
Fri 27 May 2016
SRR939828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190430
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80520.2523797732594039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60620.1900057359039377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40850.12803916713421074No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32960.10330895835357616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.051.242741
GTATCAA96900.044.5095251
ATCAACG122300.035.421023
TATCAAC122900.035.3617972
TCAACGC124250.034.9424744
CAACGCA125150.034.4291735
AACGCAG129850.033.4370966
ACGCAGA148550.028.6579237
CGCAGAG149900.028.2738638
GTACATG118350.026.9841611
TACATGG118250.026.8880792
ACATGGG121050.025.4121153
TGGTATC27000.024.9107322
GCAGAGT163750.024.3890659
TAAGGTG14350.023.273865
GTACCGT2555.5479177E-1022.1365436
CATGGGG66300.021.7807674
GTGGTAT33850.021.3978181
CAGAGTA154900.020.7397710-11
GAGTACT99850.020.56161712-13
Produced by FastQC (version 0.11.5)