FastQCFastQC Report
Fri 27 May 2016
SRR939828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190430
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.28419366668442814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74940.23488996780998173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50690.15888140470093373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53900.051.772931
GTATCAA96400.041.698511
ATCAACG122400.032.3966983
TATCAAC124350.032.0512922
TCAACGC125350.031.672114
CAACGCA128250.030.9559385
AACGCAG132350.030.032816
ACGCAGA153100.025.8074877
CGCAGAG156350.025.2406948
GTACATG120200.023.4133951
TACATGG121350.022.7559642
ACATGGG119900.022.3514213
GCAGAGT177100.021.8012669
TAAGGTG16150.020.852675
GAGTACT99850.020.4269312-13
TGGTATC28300.020.2866782
AGAGTAC155600.020.02788410-11
CAGAGTA165650.019.60023510-11
GTACTTT104300.019.55540714-15
GTAAGGT15500.019.2790094
Produced by FastQC (version 0.11.5)