##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939828_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3190430 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39514078039637 31.0 31.0 34.0 30.0 34.0 2 31.625316023231978 31.0 31.0 34.0 30.0 34.0 3 31.666882833975357 31.0 31.0 34.0 30.0 34.0 4 35.483264638308945 37.0 35.0 37.0 33.0 37.0 5 35.213484389251605 37.0 35.0 37.0 32.0 37.0 6 35.23412925530415 37.0 35.0 37.0 32.0 37.0 7 35.142996085167205 37.0 35.0 37.0 32.0 37.0 8 35.09572283359923 37.0 35.0 37.0 32.0 37.0 9 36.687725479010666 39.0 35.0 39.0 32.0 39.0 10-11 36.550043411076246 38.0 35.0 39.0 32.0 39.0 12-13 36.47006328300574 38.0 35.0 39.0 32.0 39.0 14-15 37.498726974106944 39.0 36.0 41.0 32.0 41.0 16-17 37.42159818582448 39.0 36.0 41.0 32.0 41.0 18-19 37.403153806853624 39.0 36.0 41.0 32.0 41.0 20-21 37.3139197537636 39.0 36.0 40.5 31.0 41.0 22-23 37.194330231348125 39.0 36.0 40.0 31.0 41.0 24-25 37.0478045279163 39.0 36.0 40.0 31.0 41.0 26-27 36.74667051149845 39.0 35.5 40.0 30.0 41.0 28-29 36.52488536028059 38.0 35.0 40.0 30.0 41.0 30-31 36.25475265089659 38.0 35.0 40.0 30.0 41.0 32-33 36.23242979786424 38.0 35.0 40.0 30.0 41.0 34-35 36.09059468472902 38.0 35.0 40.0 30.0 41.0 36-37 36.13498070792966 38.0 35.0 40.0 30.0 41.0 38-39 36.037211911873946 38.0 35.0 40.0 29.0 41.0 40-41 35.94695307529079 38.0 35.0 40.0 29.0 41.0 42-43 35.72907178656169 38.0 34.5 40.0 28.5 41.0 44-45 35.49471199806922 38.0 34.0 40.0 27.0 41.0 46-47 35.233366192018 38.0 34.0 40.0 27.0 41.0 48-49 34.93494669997461 38.0 33.5 40.0 26.0 41.0 50-51 34.71103174180283 38.0 33.0 40.0 25.5 41.0 52-53 34.46215682525553 38.0 33.0 40.0 24.0 41.0 54-55 34.19638732083136 37.5 33.0 40.0 24.0 41.0 56-57 33.841729954896365 37.0 32.5 40.0 23.5 41.0 58-59 33.4792024899465 37.0 32.0 39.5 23.0 41.0 60-61 33.17180818886482 36.0 31.5 39.0 22.0 41.0 62-63 32.87857451816839 36.0 31.0 39.0 22.0 40.0 64-65 32.449884655046496 35.5 31.0 39.0 20.5 40.0 66-67 31.545172594289795 35.0 30.0 38.0 15.5 40.0 68-69 29.643959278216414 34.0 26.5 37.0 8.5 40.0 70-71 28.99085389743702 33.5 25.5 37.0 8.5 39.0 72-73 29.554746225430428 34.0 27.5 36.0 9.0 39.0 74-75 29.659717498895134 34.0 28.0 36.0 8.0 39.0 76-77 29.200582680077606 32.5 27.5 35.0 8.0 37.5 78-79 29.738342637199374 34.0 29.0 35.0 8.0 37.5 80-81 29.547960933165747 34.0 29.0 35.0 7.0 37.0 82-83 29.096516457029303 34.0 28.5 35.0 6.0 36.5 84-85 28.73512786677658 33.0 28.0 35.0 2.0 36.0 86-87 28.351873571900967 33.0 27.5 35.0 2.0 36.0 88-89 27.88922872465467 33.0 26.5 35.0 2.0 35.0 90-91 27.39797174675514 33.0 26.0 35.0 2.0 35.0 92-93 26.902069626978182 32.0 25.5 34.5 2.0 35.0 94-95 26.09078729199512 32.0 24.0 34.0 2.0 35.0 96-97 25.566369580276014 32.0 23.0 34.0 2.0 35.0 98-99 24.900145278222684 31.5 19.0 34.0 2.0 35.0 100-101 23.41665073360017 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28131313846006734 1101 2 0.2682193954594183 1101 3 0.2639918649479931 1101 4 0.20726401079222256 1101 5 0.22001649101628118 1101 6 0.15791991532411487 1101 7 0.1034200991038503 1101 8 0.20044824341778167 1101 9 0.3330503554605855 1101 10-11 0.3301831711514396 1101 12-13 0.3950556199974571 1101 14-15 0.3518494850135667 1101 16-17 0.4678135504498542 1101 18-19 0.4374787013651442 1101 20-21 0.451781596633829 1101 22-23 0.34184811757095446 1101 24-25 0.3911959914500116 1101 26-27 0.44990999994902836 1101 28-29 0.28840162692253557 1101 30-31 0.23385802179642212 1101 32-33 0.004385301536885322 1101 34-35 0.011703941437424703 1101 36-37 0.151097686815568 1101 38-39 0.20701330168586907 1101 40-41 0.16634194325885687 1101 42-43 0.037545937223718795 1101 44-45 0.03755721182656657 1101 46-47 0.02278566193805176 1101 48-49 0.195576673995852 1101 50-51 0.034852814797709186 1101 52-53 -0.1412695786697995 1101 54-55 -0.3224663541579744 1101 56-57 -0.44654027260185103 1101 58-59 -0.37171030304561725 1101 60-61 -0.5005576690253832 1101 62-63 -0.3765883581872629 1101 64-65 -0.33876743575786605 1101 66-67 -0.5133701678843998 1101 68-69 -9.080877057119878 1101 70-71 -6.704070929359322 1101 72-73 -1.9578361994880318 1101 74-75 -0.690254652842043 1101 76-77 -0.4836041028767042 1101 78-79 -0.5447543869046427 1101 80-81 -0.5527446992983798 1101 82-83 -0.5419908757325373 1101 84-85 -0.43911580795098004 1101 86-87 -0.5672570779340838 1101 88-89 -0.5799623021195579 1101 90-91 -0.8457937588414985 1101 92-93 -0.9981031221538039 1101 94-95 -0.6957485981326066 1101 96-97 -0.9089228764059989 1101 98-99 -0.6346677572378994 1101 100-101 -0.6586904301224799 1102 1 0.19922197453081836 1102 2 0.15807686545310062 1102 3 0.2684399066409817 1102 4 0.16925252153132675 1102 5 0.3278315573460304 1102 6 0.2762014614390864 1102 7 0.292670730815793 1102 8 0.19291191998127033 1102 9 0.42599077712830535 1102 10-11 0.40912643552288586 1102 12-13 0.35539990300440394 1102 14-15 0.4176405068076221 1102 16-17 0.39949548348838704 1102 18-19 0.41440522537020286 1102 20-21 0.4326823783773506 1102 22-23 0.5036798847851074 1102 24-25 0.3994496040526627 1102 26-27 0.3491852092036112 1102 28-29 0.3583957046230992 1102 30-31 0.22684503221967134 1102 32-33 0.1578880455356213 1102 34-35 0.0857074158531006 1102 36-37 0.12022098908973078 1102 38-39 0.29256575351782743 1102 40-41 0.3534687396896885 1102 42-43 0.3409330976785512 1102 44-45 0.22385459022201815 1102 46-47 0.24312165372580097 1102 48-49 0.18526091673115985 1102 50-51 0.1904172099335284 1102 52-53 0.10928573560689614 1102 54-55 -0.14372316480231717 1102 56-57 0.07545583842152581 1102 58-59 -0.022885089842837658 1102 60-61 0.13295099727277915 1102 62-63 0.18889401310143228 1102 64-65 0.22490340283972898 1102 66-67 0.49277786986038663 1102 68-69 -3.9510496921483025 1102 70-71 0.5133451713987363 1102 72-73 0.6469783338347774 1102 74-75 0.4652976015799446 1102 76-77 0.27448338922689786 1102 78-79 0.05260199733414339 1102 80-81 0.09212254248936702 1102 82-83 0.09452691090675103 1102 84-85 -0.020761812372963817 1102 86-87 -0.1984664114779875 1102 88-89 -0.26586041882833555 1102 90-91 -0.2035301572938053 1102 92-93 -0.09032743911527064 1102 94-95 0.16883476810239273 1102 96-97 -0.21998800195875035 1102 98-99 0.22756169238307322 1102 100-101 0.07284018816557847 1103 1 0.2427277404981112 1103 2 0.28420749643119336 1103 3 0.2556386878813406 1103 4 0.2627592517072017 1103 5 0.3298374968708728 1103 6 0.26382015551499194 1103 7 0.10510940715025896 1103 8 0.21485267900626326 1103 9 0.3872611219292921 1103 10-11 0.3859020256185275 1103 12-13 0.35082569751227055 1103 14-15 0.40710970998565443 1103 16-17 0.39686743658928236 1103 18-19 0.35740960287714785 1103 20-21 0.4227436056220242 1103 22-23 0.3097558451368556 1103 24-25 0.2607475734294056 1103 26-27 0.3152161892252039 1103 28-29 0.2337260424817913 1103 30-31 0.3301961391311181 1103 32-33 0.37057273968866156 1103 34-35 0.19524690983643467 1103 36-37 0.1432215097623697 1103 38-39 0.41202526639965953 1103 40-41 0.3657714144042785 1103 42-43 0.2931444136304151 1103 44-45 0.4482725939245782 1103 46-47 0.2793163081534473 1103 48-49 0.191426885953625 1103 50-51 0.20329624032538618 1103 52-53 0.28925999123786994 1103 54-55 0.26280930010049275 1103 56-57 0.20806493862207986 1103 58-59 0.11527221114450725 1103 60-61 -0.058327784353259915 1103 62-63 0.08730957735628664 1103 64-65 0.3127970892019647 1103 66-67 0.3712563005145064 1103 68-69 -3.8526516764895575 1103 70-71 0.6290539611241286 1103 72-73 0.8217932539983046 1103 74-75 0.5025239109751887 1103 76-77 0.31130304365454364 1103 78-79 0.31308299278308027 1103 80-81 0.2840852372595002 1103 82-83 0.12350605507521806 1103 84-85 0.055933459148175046 1103 86-87 0.03528262431343521 1103 88-89 -0.07468114352750277 1103 90-91 -0.02560605015094808 1103 92-93 0.05143826784245675 1103 94-95 0.11825677327054862 1103 96-97 -0.26428369580323974 1103 98-99 -0.023576700474070122 1103 100-101 -0.12011438796833218 1104 1 0.17150189738446997 1104 2 0.21296515601222055 1104 3 0.23741981803725665 1104 4 0.18942524151299267 1104 5 0.28473386409824997 1104 6 0.26562467790715516 1104 7 0.2588038655545617 1104 8 0.2564102395657528 1104 9 0.36609074275617104 1104 10-11 0.22980461685499876 1104 12-13 0.2600057691822215 1104 14-15 0.3302588042411756 1104 16-17 0.3687354773777898 1104 18-19 0.2727453376525091 1104 20-21 0.3153033491992261 1104 22-23 0.33296255740960845 1104 24-25 0.16724725719962663 1104 26-27 0.27470045706489543 1104 28-29 0.36582180453144275 1104 30-31 0.2872516736801032 1104 32-33 0.22115296877118595 1104 34-35 0.1983494873471372 1104 36-37 0.2606959929088575 1104 38-39 0.30483378934123806 1104 40-41 0.26651730942693064 1104 42-43 0.19512096049553662 1104 44-45 0.16705005310011245 1104 46-47 0.18746228159014322 1104 48-49 0.054056793984450735 1104 50-51 0.07295645145507024 1104 52-53 -0.04815802776004574 1104 54-55 -0.004381057026584756 1104 56-57 0.08988941426937203 1104 58-59 0.0069156472485119025 1104 60-61 -0.11090464930527588 1104 62-63 0.0053437056617724465 1104 64-65 0.10797503055130875 1104 66-67 0.2335977885129985 1104 68-69 -4.197837663810386 1104 70-71 0.5135902638215875 1104 72-73 0.7756920998751049 1104 74-75 0.31814375762943925 1104 76-77 0.3152327017841863 1104 78-79 -0.03660548624361226 1104 80-81 -0.011120325292484523 1104 82-83 -0.03822395794445299 1104 84-85 -0.0843153183307983 1104 86-87 -0.047393809889232585 1104 88-89 -0.06305044899943724 1104 90-91 0.061950145562899905 1104 92-93 -0.0863348768635106 1104 94-95 0.17443565986835452 1104 96-97 -0.38490011431744264 1104 98-99 -0.06770397558209851 1104 100-101 -0.047346423887582034 1105 1 0.101639535880512 1105 2 0.08594879796843813 1105 3 0.13030340357349957 1105 4 0.12034723012028792 1105 5 0.12540057575093755 1105 6 0.1611957099053427 1105 7 0.09568453449677605 1105 8 0.19094872979722055 1105 9 0.13408318510376915 1105 10-11 0.12155933429588828 1105 12-13 0.12303793981421762 1105 14-15 0.08372371232909614 1105 16-17 0.20868260005280348 1105 18-19 0.0717307621800174 1105 20-21 0.23776098267182988 1105 22-23 0.13720174061332813 1105 24-25 0.1848416669361015 1105 26-27 0.16207152055329743 1105 28-29 0.2506464714942709 1105 30-31 0.23126412731169665 1105 32-33 0.23872872805096534 1105 34-35 0.11762459376721068 1105 36-37 0.028635482954761926 1105 38-39 0.17116142069574636 1105 40-41 0.16627644674490227 1105 42-43 0.038636318537442094 1105 44-45 0.09189906874961906 1105 46-47 0.07683714452334556 1105 48-49 0.22150612378637646 1105 50-51 0.13956023140592322 1105 52-53 0.08215191658848653 1105 54-55 0.2239695115780478 1105 56-57 -4.519453208118307E-4 1105 58-59 0.17721268623078146 1105 60-61 0.2413261889905698 1105 62-63 0.21980371402167975 1105 64-65 0.299000412042183 1105 66-67 0.8907197793695936 1105 68-69 1.895377646808754 1105 70-71 2.359351689803642 1105 72-73 1.418645320031093 1105 74-75 0.8786114436044805 1105 76-77 0.4666459659140152 1105 78-79 0.41847910622985296 1105 80-81 0.41142948267341595 1105 82-83 0.31857886642822564 1105 84-85 0.19677269682949472 1105 86-87 0.1737230056999266 1105 88-89 0.40740549374834956 1105 90-91 0.29455170620312643 1105 92-93 0.19859414830804312 1105 94-95 0.6171409414152755 1105 96-97 0.377829290986039 1105 98-99 0.4149859638554645 1105 100-101 0.4653028031962556 1106 1 0.3866942846573984 1106 2 0.39776832114930016 1106 3 0.3597531977802255 1106 4 0.24054603418955622 1106 5 0.2987018105105861 1106 6 0.3805092146036415 1106 7 0.3665473130942729 1106 8 0.411772679474943 1106 9 0.37865296067261056 1106 10-11 0.4041620367887049 1106 12-13 0.3819821814854194 1106 14-15 0.5121697789595245 1106 16-17 0.5106347239947198 1106 18-19 0.5286180418259008 1106 20-21 0.48251228344064145 1106 22-23 0.4847914354180176 1106 24-25 0.48634240468557977 1106 26-27 0.5865250035074041 1106 28-29 0.588830831205378 1106 30-31 0.7303458233589097 1106 32-33 0.5978583778141058 1106 34-35 0.6547896943341058 1106 36-37 0.7161743118745392 1106 38-39 0.7205780161760558 1106 40-41 0.8100609074111986 1106 42-43 0.7152448065393102 1106 44-45 0.7648197800954364 1106 46-47 0.7805346284093488 1106 48-49 0.7429804578704022 1106 50-51 0.838000279553782 1106 52-53 0.8518925394043606 1106 54-55 0.7976949522862853 1106 56-57 0.7545084491810172 1106 58-59 0.7591679164862768 1106 60-61 0.7644798738550591 1106 62-63 0.772329535253327 1106 64-65 0.6864421686625164 1106 66-67 1.2186659236578556 1106 68-69 2.7601627287377752 1106 70-71 3.107811152411397 1106 72-73 1.946422558518318 1106 74-75 1.4346745794280444 1106 76-77 0.8891036423914542 1106 78-79 0.8766506523974371 1106 80-81 0.8902890772994887 1106 82-83 1.0897464520459934 1106 84-85 1.018549896487798 1106 86-87 1.028361120167581 1106 88-89 1.1584334893995099 1106 90-91 1.1713650167568268 1106 92-93 1.2763636210113134 1106 94-95 1.5548098472163154 1106 96-97 1.3023992606994774 1106 98-99 1.4860525942818086 1106 100-101 1.5822962799377294 1107 1 0.06068563024857809 1107 2 0.14282899440687302 1107 3 0.17184319131251513 1107 4 0.07015504586222931 1107 5 0.0795681753509001 1107 6 0.14592545778945265 1107 7 0.1155448184070309 1107 8 0.15929878129514208 1107 9 0.12133521143487513 1107 10-11 0.17622269464697382 1107 12-13 0.12133664266377053 1107 14-15 0.1874800749341503 1107 16-17 0.07576069030104549 1107 18-19 0.04813165481058945 1107 20-21 0.12279262776907984 1107 22-23 0.15508271325226275 1107 24-25 0.16670059249548785 1107 26-27 0.217255084507741 1107 28-29 0.2851338433092323 1107 30-31 0.29544676579631357 1107 32-33 0.1588996787473036 1107 34-35 0.2979762723007866 1107 36-37 0.0554802652454498 1107 38-39 0.138051991352377 1107 40-41 0.06437848725659734 1107 42-43 0.040563572131098624 1107 44-45 0.21409485786191595 1107 46-47 0.2251183082270174 1107 48-49 0.16160938084308185 1107 50-51 0.17949947878997818 1107 52-53 0.08760006042441404 1107 54-55 0.1742090888473129 1107 56-57 0.20338353002735943 1107 58-59 0.11560867536397268 1107 60-61 0.1301563652776494 1107 62-63 0.2754378847559309 1107 64-65 0.15318275198814746 1107 66-67 0.7236252694954253 1107 68-69 2.0904196661242054 1107 70-71 2.329091065559318 1107 72-73 1.299674121547671 1107 74-75 0.72429737347775 1107 76-77 0.4716731702487955 1107 78-79 0.4624906074314694 1107 80-81 0.4000441513701425 1107 82-83 0.5879070051889919 1107 84-85 0.5672567399384754 1107 86-87 0.4790868123662939 1107 88-89 0.4704561109770147 1107 90-91 0.2655175160638592 1107 92-93 0.3120946259698876 1107 94-95 0.6533850266182064 1107 96-97 0.5020204378222708 1107 98-99 0.8262451625777203 1107 100-101 0.8866666023991563 1108 1 -0.05083741846706147 1108 2 -0.15285883475908335 1108 3 -0.09498423703891845 1108 4 -0.09236056197882903 1108 5 0.006423176018358845 1108 6 3.554664406024699E-4 1108 7 -0.037789635962020895 1108 8 -0.06101654318938898 1108 9 -0.11445206539973185 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-0.5157273064439991 2316 58-59 -0.6066312695494389 2316 60-61 -0.5344806194753815 2316 62-63 -0.5799572246736346 2316 64-65 -0.449159097160269 2316 66-67 -0.023496962046703374 2316 68-69 1.2506384955045107 2316 70-71 1.491119468464749 2316 72-73 0.6860862953262163 2316 74-75 0.25991366209338906 2316 76-77 -0.17147015600537685 2316 78-79 -0.32490067687123414 2316 80-81 -0.4376058346499967 2316 82-83 -0.41076304936219543 2316 84-85 -0.43023766811868214 2316 86-87 -0.38455901538996784 2316 88-89 -0.48089212726354447 2316 90-91 -0.4923149219637324 2316 92-93 -0.5043368370370303 2316 94-95 -0.028422643266800662 2316 96-97 -0.09649739988050854 2316 98-99 -0.26748606128440855 2316 100-101 -0.3887693014432223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 51.0 5 129.0 6 273.0 7 750.0 8 1850.0 9 3199.0 10 5203.0 11 8051.0 12 10956.0 13 14000.0 14 17380.0 15 21209.0 16 25699.0 17 29537.0 18 27891.0 19 24469.0 20 24327.0 21 25191.0 22 27269.0 23 31367.0 24 37857.0 25 46086.0 26 57307.0 27 71256.0 28 87098.0 29 105304.0 30 126224.0 31 149806.0 32 179254.0 33 216550.0 34 269019.0 35 338829.0 36 427026.0 37 474355.0 38 280767.0 39 24866.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.91576517179348 19.76673682996092 14.679521786047726 23.637976212197877 2 15.623706750605084 20.97148894546093 39.641432888978066 23.76337141495592 3 19.12815459242459 24.80595080033149 30.212184697476324 25.85370990976759 4 13.082813288490893 17.191287694762146 37.176054638402974 32.549844378343984 5 13.235331914506823 38.03277301178838 34.64275975338747 14.089135320317325 6 31.849331813787995 37.038290787317564 17.305201684538876 13.807175714355566 7 28.23484608657767 32.89337173985952 20.902825011048666 17.968957162514144 8 26.157633923953828 35.85544895202214 19.68339690888062 18.30352021514341 9 26.31356901734249 16.54651567343587 20.044351388370846 37.09556392085079 10-11 24.7381544180565 26.860078422030888 27.675125296590114 20.7266418633225 12-13 25.646448911275282 23.923734418244564 28.099770250405122 22.330046420075035 14-15 22.29119585761167 25.55147738706068 26.126243170983223 26.03108358434443 16-17 21.8929673668306 28.811885082823746 26.953728185274695 22.34141936507096 18-19 21.955849211548287 27.2773732694339 29.170064850192606 21.596712668825205 20-21 22.53834122673119 26.501114269863308 29.320718523835342 21.63982597957015 22-23 22.204499080061307 26.389138768128433 29.274517854959992 22.131844296850268 24-25 22.05941214728445 26.672355563913897 29.317520393051083 21.950711895750565 26-27 21.688908767839727 27.032028061500345 29.09467120483472 22.1843919658252 28-29 21.80213893099839 27.274763193812234 29.049166012495824 21.87393186269355 30-31 21.93187153767505 26.940829622738615 28.896312516181283 22.230986323405048 32-33 21.68626246982503 27.032726644697302 28.850457386905486 22.430553498572177 34-35 22.218157802103697 27.068066289960747 28.775718297444712 21.938057610490844 36-37 22.301442580658524 26.66793534987025 28.878675971668304 22.151946097802924 38-39 21.847016262893217 26.981289570297495 28.910328582008553 22.26136558480074 40-41 22.323332366386847 26.778607587648416 28.90996368538082 21.988096360583913 42-43 21.95284618994284 26.791291432397742 28.810963197297983 22.444899180361432 44-45 21.956380037258555 26.983916070202962 28.923525835866258 22.13617805667222 46-47 22.269831566454094 27.14102828967627 28.31201422978199 22.277125914087645 48-49 21.938450726540577 27.355997822652885 28.28563719561456 22.419914255191983 50-51 22.127459851138298 27.35201989842467 28.23983148584358 22.280688764593453 52-53 22.378182713578713 27.116386411952863 28.188226400535125 22.31720447393329 54-55 21.857486114347708 27.32201446322141 28.3496107563662 22.47088866606469 56-57 21.90540438001795 27.499606496970188 28.08131685240219 22.513672270609682 58-59 21.86074987300131 27.087939207399643 28.204446184608212 22.84686473499084 60-61 21.6444076713404 27.368055286714487 28.363139034777074 22.62439800716804 62-63 21.697710165805635 27.228320798705603 28.518456526249995 22.555512509238763 64-65 21.41484212713897 27.216851181581923 28.35868709009964 23.009619601179466 66-67 21.279516930050917 27.372624361944588 28.33405536406045 23.013803343944044 68-69 21.54408133472958 27.076434337930934 28.690504181023346 22.688980146316144 70-71 21.426808121712725 27.014753405300677 28.392135440128026 23.166303032858576 72-73 21.356114277137273 27.292903972142106 28.14109753166144 23.20988421905918 74-75 21.591524394392444 27.593892176255526 27.65633176285937 23.158251666492657 76-77 21.511898423973808 27.410881748183208 27.806597073945245 23.270622753897737 78-79 21.801609464829276 27.49813259324574 27.568879333394563 23.13137860853042 80-81 21.802698614396164 27.639044652389156 27.31851836992356 23.23973836329112 82-83 21.824548305117684 27.59534144008265 27.398941458638156 23.18116879616152 84-85 21.706063317781542 27.620309583549474 27.323341947677655 23.35028515099133 86-87 21.67713038217898 27.607110729409257 27.290237243722554 23.425521644689205 88-89 21.62843085599048 27.62155840874919 27.10192479299754 23.648085942262792 90-91 21.540892380323413 27.67325275646304 27.13593953882431 23.64991532438924 92-93 21.639740899535802 27.40138959871274 27.211284796583946 23.74758470516751 94-95 21.23931656104782 27.27044447748628 27.502338546205348 23.98790041526056 96-97 21.5232841704257 26.972015482757765 27.481268051278562 24.02343229553797 98-99 21.381288811442126 27.228595975091707 27.129563462238092 24.26055175122807 100-101 21.187000537441186 27.199226109767466 27.341854129778646 24.271919223012702 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 611.0 1 412.0 2 267.5 3 347.0 4 335.0 5 312.0 6 557.0 7 1184.0 8 1906.0 9 2230.0 10 2059.0 11 1886.0 12 1973.0 13 2138.5 14 2339.5 15 2554.0 16 2840.5 17 3199.0 18 3547.0 19 4021.0 20 4672.5 21 5587.0 22 6864.5 23 8473.0 24 10496.0 25 13142.5 26 17148.0 27 22396.0 28 27111.0 29 31791.0 30 37031.0 31 44588.0 32 54884.0 33 60823.5 34 63859.0 35 70441.5 36 79763.5 37 87456.0 38 92673.5 39 97141.0 40 101453.0 41 106874.0 42 110581.0 43 112902.0 44 116143.5 45 120378.5 46 121765.5 47 121977.0 48 122055.0 49 120046.5 50 118325.5 51 115820.0 52 111643.5 53 108443.0 54 105965.5 55 98275.5 56 88256.0 57 79646.0 58 71997.5 59 62245.5 60 49679.5 61 39837.5 62 32251.5 63 23478.0 64 16051.5 65 11675.0 66 8513.5 67 6352.0 68 4778.0 69 3529.0 70 2637.0 71 1811.0 72 1218.0 73 951.5 74 708.0 75 508.5 76 353.0 77 228.5 78 135.5 79 70.5 80 48.0 81 29.0 82 15.5 83 9.5 84 3.0 85 1.0 86 3.0 87 2.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022818240801396678 2 0.024761552517999143 3 6.268747472911175E-5 4 0.0 5 0.0 6 6.268747472911175E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.291092423278367E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5671868682277937E-4 26-27 2.8209363628100285E-4 28-29 1.4104681814050142E-4 30-31 5.01499797832894E-4 32-33 0.002460483383117636 34-35 0.00448215444313149 36-37 0.10652169143344314 38-39 0.0985290384054814 40-41 0.10724259739282793 42-43 0.10896650294787849 44-45 0.10163206840457242 46-47 0.09498719608328658 48-49 0.09606855502236375 50-51 0.09534764906297898 52-53 0.07546004770516827 54-55 0.06478750513253699 56-57 0.03535573574721902 58-59 0.013681541359628641 60-61 0.009293418128590818 62-63 0.002131374140789799 64-65 0.005422466564068166 66-67 0.1557783747018427 68-69 0.4180314252310817 70-71 0.3934736070059522 72-73 0.18300041060295946 74-75 0.02968251928423441 76-77 0.03184523716238877 78-79 0.00554784151352639 80-81 0.0016298743429569054 82-83 6.268747472911175E-5 84-85 4.701560604683381E-4 86-87 0.001253749494582235 88-89 2.8209363628100285E-4 90-91 5.955310099265616E-4 92-93 0.0026485458073049715 94-95 0.07998921775434659 96-97 0.01752114918678673 98-99 0.017818914691750017 100-101 0.009826261663788267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3190430.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.685714215180234 #Duplication Level Percentage of deduplicated Percentage of total 1 71.23683102092492 34.682159966798366 2 14.743268372802563 14.355731011919415 3 5.737726965318632 8.380360058347087 4 2.775079753120398 5.404269591390103 5 1.5532750220427778 3.781115191037623 6 0.9639317318344132 2.8157822891420397 7 0.6041542153579869 2.0589575629570778 8 0.4212268917199293 1.6406185656020118 9 0.3148639865595112 1.379644025965984 >10 1.4751085098653125 12.855044118778682 >50 0.10757508435928248 3.6370599705004376 >100 0.060719228639026894 5.280108953551513 >500 0.003730666108069664 1.2029865482389661 >1k 0.002315585858872428 1.8488069713146291 >5k 1.9296548823936898E-4 0.6773551744560355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9067 0.28419366668442814 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7494 0.23488996780998173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5069 0.15888140470093373 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.1343737364555876E-5 0.0 5 0.0 0.0 0.0 3.1343737364555876E-5 0.0 6 0.0 0.0 0.0 3.1343737364555876E-5 0.0 7 0.0 0.0 0.0 3.1343737364555876E-5 0.0 8 0.0 0.0 0.0 6.268747472911175E-5 0.0 9 0.0 0.0 0.0 6.268747472911175E-5 0.0 10-11 0.0 0.0 0.0 6.268747472911175E-5 0.0 12-13 0.0 0.0 0.0 9.403121209366763E-5 0.0 14-15 0.0 0.0 0.0 1.5671868682277937E-4 0.0 16-17 0.0 0.0 0.0 2.0373429286961318E-4 0.0 18-19 0.0 0.0 0.0 2.977655049632808E-4 0.0 20-21 0.0 0.0 0.0 4.7015606046833813E-4 0.0 22-23 0.0 0.0 0.0 8.149371714784527E-4 0.0 24-25 0.0 0.0 0.0 0.001253749494582235 0.0 26-27 0.0 0.0 0.0 0.002100030403425244 0.0 28-29 0.0 0.0 0.0 0.006597856715239011 0.0 30-31 0.0 0.0 0.0 0.02347645928605235 0.0 32-33 0.0 0.0 0.0 0.06463078644571421 0.0 34-35 0.0 0.0 0.0 0.12539062132690576 0.0 36-37 0.0 0.0 0.0 0.2264428305902339 0.0 38-39 0.0 0.0 0.0 0.3900728115018979 3.1343737364555876E-5 40-41 0.0 0.0 0.0 0.5661462561472905 3.1343737364555876E-5 42-43 0.0 0.0 0.0 0.7386778584704883 3.1343737364555876E-5 44-45 0.0 0.0 0.0 0.9506242105296151 3.1343737364555876E-5 46-47 0.0 0.0 0.0 1.1975501734875862 3.1343737364555876E-5 48-49 0.0 0.0 0.0 1.4600226301783772 3.1343737364555876E-5 50-51 0.0 0.0 0.0 1.7452036245897888 3.1343737364555876E-5 52-53 0.0 0.0 0.0 2.017063530621264 3.1343737364555876E-5 54-55 0.0 0.0 0.0 2.305629648668048 3.1343737364555876E-5 56-57 0.0 0.0 0.0 2.6340023131678176 3.1343737364555876E-5 58-59 0.0 0.0 0.0 2.965117554686986 3.1343737364555876E-5 60-61 0.0 0.0 0.0 3.31099569650486 3.1343737364555876E-5 62-63 0.0 0.0 0.0 3.640136282570061 3.1343737364555876E-5 64-65 1.5671868682277938E-5 0.0 0.0 3.9786486461072643 3.1343737364555876E-5 66-67 3.1343737364555876E-5 0.0 0.0 4.346389044736917 3.1343737364555876E-5 68-69 3.1343737364555876E-5 0.0 0.0 4.754954661283902 3.1343737364555876E-5 70-71 3.1343737364555876E-5 0.0 0.0 5.213623242008131 3.1343737364555876E-5 72-73 3.1343737364555876E-5 0.0 0.0 5.682525552981886 6.268747472911175E-5 74-75 3.1343737364555876E-5 0.0 0.0 6.155016721883884 6.268747472911175E-5 76-77 3.1343737364555876E-5 0.0 0.0 6.653679911485286 6.268747472911175E-5 78-79 3.1343737364555876E-5 0.0 0.0 7.2197164645518 6.268747472911175E-5 80-81 3.1343737364555876E-5 0.0 0.0 7.8072548214504 6.268747472911175E-5 82-83 3.1343737364555876E-5 0.0 0.0 8.407189626476683 6.268747472911175E-5 84-85 3.1343737364555876E-5 0.0 0.0 8.990715984992619 6.268747472911175E-5 86-87 3.1343737364555876E-5 0.0 0.0 9.57378785931677 6.268747472911175E-5 88-89 3.1343737364555876E-5 0.0 0.0 10.199393185244622 6.268747472911175E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5390 0.0 51.77293 1 GTATCAA 9640 0.0 41.69851 1 ATCAACG 12240 0.0 32.396698 3 TATCAAC 12435 0.0 32.051292 2 TCAACGC 12535 0.0 31.67211 4 CAACGCA 12825 0.0 30.955938 5 AACGCAG 13235 0.0 30.03281 6 ACGCAGA 15310 0.0 25.807487 7 CGCAGAG 15635 0.0 25.240694 8 GTACATG 12020 0.0 23.413395 1 TACATGG 12135 0.0 22.755964 2 ACATGGG 11990 0.0 22.351421 3 GCAGAGT 17710 0.0 21.801266 9 TAAGGTG 1615 0.0 20.85267 5 GAGTACT 9985 0.0 20.42693 12-13 TGGTATC 2830 0.0 20.286678 2 AGAGTAC 15560 0.0 20.027884 10-11 CAGAGTA 16565 0.0 19.600235 10-11 GTACTTT 10430 0.0 19.555407 14-15 GTAAGGT 1550 0.0 19.279009 4 >>END_MODULE